Abstract:
:To formulate a 'logic' for how a single immunoglobulin variable region gene generates antibodies with different antigen specificity and polyreactivity, we analysed chimeric antibodies produced in transgenic mice carrying the germ-line human V3-23 gene, multiple diversity (D) and joining (J) gene segments. Hybridomas producing antibodies encoded by the V3-23 gene in combination with different mouse Vkappa genes were obtained by fusion of splenocytes from transgenic mice. All antibodies had human mu-chains and mouse light chains, were multimeric in structure and expressed the human V3-23 gene. Nucleotide sequence analyses of genes encoding the heavy and light chains of 12 antibodies in relation to antigen specificity highlighted the importance of heavy chain variable region CDR3 in determining reactivity with different antigens. However, the results also suggest that non-CDR3 sequences intrinsic to the V3-23 gene itself may be involved in, or determine, the binding of the chimeric antibodies to some of the antigens tested in the current study.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Mageed RA,Harmer IJ,Wynn SL,Moyes SP,Maziak BB,Brüggemann M,MacKworth-Young CGdoi
10.1046/j.1365-2249.2001.01380.xsubject
Has Abstractpub_date
2001-01-01 00:00:00pages
1-8issue
1eissn
0009-9104issn
1365-2249pii
cei1380journal_volume
123pub_type
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journal_title:Clinical and experimental immunology
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doi:
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pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
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更新日期:1987-02-01 00:00:00
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