Abstract:
:Integral membrane proteins (IMP) represent a serologically distinct class of mycobacterial antigens which are potent stimulators of human T cells (Mehrotra et al., Clin Exp Immunol 1995; 102:626). The range of IMP from Mycobacterium fortuitum was resolved by continuous elution SDS-PAGE to recover 31 discrete fractions covering bands up to approximately 58 kD. The fractions, after removal of SDS, were subjected to human T cell proliferation assays for the identification of immunodominant molecule(s). A low molecular weight (<20kD) fraction was able to stimulate T cells from 11 out of 12 donors comprising mainly tuberculoid leprosy patients. The described protocol is well suited to situations where large quantities of antigenic protein mixtures must be processed in order to get the purified molecules/fractions in amounts required for immunoepidemiological studies.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Mehrotra J,Mittal A,Dhindsa MS,Sinha Sdoi
10.1046/j.1365-2249.1997.4531351.xsubject
Has Abstractpub_date
1997-09-01 00:00:00pages
446-50issue
3eissn
0009-9104issn
1365-2249journal_volume
109pub_type
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