Desialylation of dying cells with catalytically active antibodies possessing sialidase activity facilitate their clearance by human macrophages.


:Recently we reported the first known incidence of antibodies possessing catalytic sialidase activity (sialidase abzymes) in the serum of patients with multiple myeloma and systemic lupus erythematosus (SLE). These antibodies desialylate biomolecules, such as glycoproteins, gangliosides and red blood cells. Desialylation of dying cells was demonstrated to facilitate apoptotic cell clearance. In this study we assessed the possibility to facilitate dying cell clearance with the use of F(ab)2 fragments of sialidase abzymes. Two sources of sialidase abzymes were used: (i) those isolated from sera of patients with SLE after preliminary screening of a cohort of patients for sialidase activity; and (ii) by creating an induced sialidase abzyme through immunization of a rabbit with synthetic hapten consisting of a non-hydrolysable analogue of sialidase reaction conjugated with bovine serum albumin (BSA) or keyhole limpet haemocyanin (KLH). Antibodies were purified by ammonium sulphate precipitation, protein-G affinity chromatography and size exclusion-high performance liquid chromatography (HPLC-SEC). Effect of desialylation on efferocytosis was studied using human polymorphonuclear leucocytes (PMN), both viable and aged, as prey, and human monocyte-derived macrophages (MoMa). Treatment of apoptotic and viable prey with both disease-associated (purified from blood serum of SLE patients) and immunization-induced (obtained by immunization of rabbits) sialidase abzymes, its F(ab)2 fragment and bacterial neuraminidase (as positive control) have significantly enhanced the clearance of prey by macrophages. We conclude that sialidase abzyme can serve as a protective agent in autoimmune patients and that artificial abzymes may be of potential therapeutic value.


Clin Exp Immunol


Tomin A,Dumych T,Tolstyak Y,Kril I,Mahorivska I,Bila E,Stoika R,Herrmann M,Kit Y,Bilyy R




Has Abstract


2015-01-01 00:00:00












  • Effect of IgG for intravenous use on Fc receptor-mediated phagocytosis by human monocytes.

    abstract::Polyspecific IgG given intravenously at high doses (IVIG) is used for immunomodulatory therapy in autoimmune diseases such as idiopathic thrombocytopenic purpura and myasthenia gravis. It is assumed that the clinical effect is brought about in part by a modulation of mononuclear phagocyte function, in particular by an...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Jungi TW,Brcic M,Kuhnert P,Spycher MO,Li F,Nydegger UE

    更新日期:1990-10-01 00:00:00

  • In vitro exposure of human fibroblasts to local anaesthetics impairs cell growth.

    abstract::Lidocaine, bupivacaine or ropivacaine are used routinely to manage perioperative pain. Sparse data exist evaluating the effects of local anaesthetics (LA) on fibroblasts, which are involved actively in wound healing. Therefore, we investigated the effects of the three LA to assess the survival, viability and prolifera...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Fedder C,Beck-Schimmer B,Aguirre J,Hasler M,Roth-Z'graggen B,Urner M,Kalberer S,Schlicker A,Votta-Velis G,Bonvini JM,Graetz K,Borgeat A

    更新日期:2010-11-01 00:00:00

  • Infiltrating cellular pattern in kidney graft biopsies translates into forkhead box protein 3 up-regulation and p16INK4α senescence protein down-regulation in patients treated with belatacept compared to cyclosporin A.

    abstract::Renal allograft survival is related directly to cell senescence. In the transplantation scenario many cellular events - participating as immunological and non-immunological factors - could contribute to accelerate this biological process, responsible for the ultimate fate of the graft. Mechanisms concerned in toleranc...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Furuzawa-Carballeda J,Lima G,Alberú J,Palafox D,Uribe-Uribe N,Morales-Buenrostro LE,Reyes Acevedo R,Mondragón G,Chevaile A,Llorente L

    更新日期:2012-02-01 00:00:00

  • Immunological monitoring of extracorporeal photopheresis after heart transplantation.

    abstract::Extracorporeal photopheresis (ECP) has been used as a prophylactic and therapeutic option to avoid and treat rejection after heart transplantation (HTx). Tolerance-inducing effects of ECP such as up-regulation of regulatory T cells (T(regs)) are known, but specific effects of ECP on regulatory T cell (T(reg)) subsets ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Dieterlen MT,Bittner HB,Pierzchalski A,Dhein S,Mohr FW,Barten MJ

    更新日期:2014-04-01 00:00:00

  • Induction of β-defensins by l-isoleucine as novel immunotherapy in experimental murine tuberculosis.

    abstract::Tuberculosis is a worldwide health problem, and multidrug-resistant (MDR) and extensively multidrug-resistant (XMDR) strains are rapidly emerging and threatening the control of this disease. These problems motivate the search for new treatment strategies. One potential strategy is immunotherapy using cationic anti-mic...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Rivas-Santiago CE,Rivas-Santiago B,León DA,Castañeda-Delgado J,Hernández Pando R

    更新日期:2011-04-01 00:00:00

  • Determination of cryoglobulins as lipoprotein-autoantibody immune complexes and antigenic determinants against antilipoprotein autoantibody.

    abstract::Serum IgG-antilipoprotein-autoantibody activity (at 4 degrees C) of a plane xanthoma patient was shown by double-immunodiffusion method. Cryoglobulins in the serum were dissociated to polyclonal IgG and alpha- and beta-lipoproteins by acidification and were reconstructed by neutralization. IgG fraction of the cryoglob...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kodama H

    更新日期:1977-06-01 00:00:00

  • Anti-DNA antibody derived from a systemic lupus erythematosus (SLE) patient forms histone-DNA-anti-DNA complexes that bind to rat glomeruli in vivo.

    abstract::Histone can mediate the binding of both free DNA and DNA complexed to anti-DNA antibody to the glomerular capillary wall. We tested whether performed histone-DNA-anti-DNA immune complexes (IC) could bind to the glomerular capillary wall. The immune complex, generated with anti-DNA antibody derived from an SLE patient ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Morioka T,Fujigaki Y,Batsford SR,Woitas R,Oite T,Shimizu F,Vogt A

    更新日期:1996-04-01 00:00:00

  • Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.

    abstract::The efficacy of steroids and immunosuppressive treatments in idiopathic nephrotic syndrome (INS) hints at the implication of immune cells in the pathophysiology of the disease. Toll-like receptor (TLR) dysfunctions are involved in many kidney diseases of immune origin, but remain little described in INS. We investigat...

    journal_title:Clinical and experimental immunology

    pub_type: 临床试验,杂志文章


    authors: Jamin A,Dehoux L,Dossier C,Fila M,Heming N,Monteiro RC,Deschênes G

    更新日期:2015-12-01 00:00:00

  • IgD production and other lymphocyte functions in HIV infection: immaturity and activation of B cells at different clinical stages.

    abstract::T and B cell function, in particular IgD production in vitro, were studied across the spectrum of HIV infection in homosexual men and compared with seronegative homosexual and heterosexual male controls. Proliferation to phytohaemagglutinin (PHA) was reduced most strikingly in symptomatic HIV infection; it was also im...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Rogers LA,Forster SM,Pinching AJ

    更新日期:1989-01-01 00:00:00

  • Prevalence in myositis of antibodies recognizing anti-U3 RNA probably in a novel complex with 22/25 kD protein and not fibrillarin.

    abstract::New antibodies against a U3 snRNP, which were named anti-Myo 22/25 antibodies, were detected in four (8%) of 53 serum samples from patients with polymyositis/dermatomyositis (PM/DM) by RNA immunoprecipitation. In the protein immunoprecipitation analysis, all four serum samples precipitated 22 kDa and 25 kDa proteins, ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kubo M,Ihn H,Kuwana M,Yamane K,Yazawa N,Tamaki K

    更新日期:2001-11-01 00:00:00

  • Enhancing effects of oral adjuvants on anti-HBs responses induced by hepatitis B vaccine.

    abstract::Hepatitis B (HB) vaccine is very promising for the prevention of HB infection. There exist, however, some non-responders to current vaccination trials. In this study, taurine, parotin and lithium were selected as adjuvants which can be administered orally. The mechanisms of these three materials as adjuvants and their...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kuriyama S,Tsujii T,Ishizaka S,Kikuchi E,Kinoshita K,Nishimura K,Kitagami K,Yoshikawa M,Matsumoto M

    更新日期:1988-06-01 00:00:00

  • Human neutrophil Fc receptor-mediated adhesion under flow: a hollow fibre model of intravascular arrest.

    abstract::Human polymorphonuclear cells (PMN) were found to adhere to a novel model of blood vessel wall-associated IgG. The internal surfaces of cellulose acetate hollow fibres, of comparable internal diameter to small blood vessels, were coated with normal serum human IgG, heat-aggregated IgG (HAIgG), laminin or fibrinogen. U...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: D'Arrigo C,Candal-Couto JJ,Greer M,Veale DJ,Woof JM

    更新日期:1995-04-01 00:00:00

  • Association of increased platelet-associated immunoglobulins with thrombocytopenia and the severity of disease in secondary dengue virus infections.

    abstract::Severe thrombocytopenia and increased vascular permeability are two major characteristics of dengue haemorrhagic fever (DHF). To develop a better understanding of the roles of platelet-associated IgG (PAIgG) and IgM (PAIgM) in inducing thrombocytopenia and its severity of disease in patients with secondary dengue viru...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Saito M,Oishi K,Inoue S,Dimaano EM,Alera MT,Robles AM,Estrella BD Jr,Kumatori A,Moji K,Alonzo MT,Buerano CC,Matias RR,Morita K,Natividad FF,Nagatake T

    更新日期:2004-11-01 00:00:00

  • Human lymphocyte markers defined by antibodies derived from somatic cell hybrids. II. A hybridoma secreting antibody against an antigen expressed by human B and null lymphocytes.

    abstract::A hybridoma (FMC4) has been derived which secretes antibody showing selective reaction with human B lymphocytes, monocytes and some null lymphocytes. Few, if any, T lymphocytes in normal blood are stained, although stimulation of lymphocytes with PHA leads to an increase in the proportion of cells reacting with the hy...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Beckman IG,Bradley J,Brooks DA,Kupa A,McNamara PJ,Thomas ME,Zola H

    更新日期:1980-06-01 00:00:00

  • Diverse T cell receptor beta gene usage by infiltrating T cells in the lacrimal glands of Sjögren's syndrome.

    abstract::Sjögren's syndrome (SS) is an autoimmune disease characterized by T cell infiltration into the salivary and lacrimal glands (LG). Previous studies on T cell receptor (TCR) usage in the minor salivary glands (SG) have yielded controversial results. We studied TCR beta gene usage of the T cells infiltrating to LG, which...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Mizushima N,Kohsaka H,Tsubota K,Saito I,Miyasaka N

    更新日期:1995-07-01 00:00:00

  • The role of stromal cells in inflammatory bone loss.

    abstract::Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation, local and systemic bone loss and a lack of compensatory bone repair. Fibroblast-like synoviocytes (FLS) are the most abundant cells of the stroma and a key population in autoimmune diseases such as RA. An increasing body of evide...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Wehmeyer C,Pap T,Buckley CD,Naylor AJ

    更新日期:2017-07-01 00:00:00

  • Synthesis of alpha 1-anti-trypsin by human monocytes.

    abstract::The present report concerns the site of synthesis of alpha 1-anti-trypsin (alpha 1 AT). In this study circulating cells and tissue fragments were incubated with radioactive amino acids to be incorporated during protein synthesis. The de novo synthesis of proteins was demonstrated by autoradiography of the immunoelectr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: van Furth R,Kramps JA,Diesselhof-den Dulk MM

    更新日期:1983-03-01 00:00:00

  • Colony-stimulating factor-1 (CSF-1) expression in the uteroplacental unit of mice with spontaneous and induced pregnancy loss.

    abstract::CSF-1 plays an important role in female reproduction and normal embryo development. To understand further CSF-1 function in normal and, especially, in compromised pregnancy, we studied the pattern of its mRNA expression as well as expression of its receptor (c-fms) in the uteroplacental units of mice with induced (cyc...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Gorivodsky M,Torchinsky A,Shepshelovich J,Savion S,Fein A,Carp H,Toder V

    更新日期:1999-09-01 00:00:00

  • MicroRNA-155 may be involved in the pathogenesis of atopic dermatitis by modulating the differentiation and function of T helper type 17 (Th17) cells.

    abstract::Our aims were to identify the differential expression of microRNA (miR)-155, as well as to explore the possible regulatory effects of miR-155 on the differentiation and function of T helper type 17 (Th17) cells in atopic dermatitis (AD). The Th17 cell percentage and expression levels of miR-155, retinoic acid-related ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ma L,Xue HB,Wang F,Shu CM,Zhang JH

    更新日期:2015-07-01 00:00:00

  • Immunodeficiencies.

    abstract::Primary immunodeficiencies (PIDs) are uncommon, chronic and severe disorders of the immune system in which patients cannot mount a sufficiently protective immune response, leading to an increased susceptibility to infections. The treatment of choice for PID patients with predominant antibody deficiency is intravenous ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Ballow M,Notarangelo L,Grimbacher B,Cunningham-Rundles C,Stein M,Helbert M,Gathmann B,Kindle G,Knight AK,Ochs HD,Sullivan K,Franco JL

    更新日期:2009-12-01 00:00:00

  • Thymic stromal lymphopoietin expression is increased in the horny layer of patients with atopic dermatitis.

    abstract::Thymic stromal lymphopoietin (TSLP) is known for its capacity to induce CD11c(+) myeloid dendritic cells to promote T helper type 2 (Th2)-skewed inflammatory responses. Although increased expression of TSLP was reported in the lesional skin of limited numbers of patients with atopic dermatitis (AD), the relationships...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Sano Y,Masuda K,Tamagawa-Mineoka R,Matsunaka H,Murakami Y,Yamashita R,Morita E,Katoh N

    更新日期:2013-03-01 00:00:00

  • Polyclonal T cell elimination by prolonged immunostimulation in an experimental model.

    abstract::An experimental model of immunological deficiency obtained by treating mice for 6 months with serum of human blood drawn from different healthy individuals has been studied. The results show that an alteration of a circulating lymphocyte population with alterations of the ratio CD4+/CD8+ appeared in mice stimulated fo...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Galdiero F,Galdiero M,Nuzzo I,Vitiello M,Bentivoglio C,Romano-Carratelli C

    更新日期:1997-11-01 00:00:00

  • Characteristics of splenic CD8+ T cell exhaustion in patients with hepatitis C.

    abstract::There is increasing interest in the role of T cell exhaustion and it is well known that the natural history of chronic hepatitis C virus infection (HCV) is modulated by CD8(+) T cell immunobiology. There are many pathways that alter the presence of exhaustive T cells and, in particular, they are functionally impaired ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Sumida K,Shimoda S,Iwasaka S,Hisamoto S,Kawanaka H,Akahoshi T,Ikegami T,Shirabe K,Shimono N,Maehara Y,Selmi C,Gershwin ME,Akashi K

    更新日期:2013-10-01 00:00:00

  • Widespread IgE-mediated hypersensitivity in Northern Sudan to the chironomid Cladotanytarsus lewisi ('green nimitti').

    abstract::Hypersensitivity to Chironomidae (non-biting midges) has been a problem in Northern Sudan since about 1927 and is probably due to the working of dams which have produced lake-like conditions on parts of the Blue and main Niles where breeding has evidently increased. Studies were undertaken to determine whether this hy...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kay AB,Gad El Rab MO,Stewart J,Erwa HH

    更新日期:1978-10-01 00:00:00

  • Immunization against Plasmodium falciparum asexual blood stages using soluble antigens.

    abstract::Saimiri sciureus monkeys have been successfully immunized against a human malaria parasite, Plasmodium falciparum, using soluble antigens purified from schizont and merozoite extracts. High levels of antibodies reacting with schizont and merozoite specific polypeptides of 140 and 200 kdaltons were detected in the sera...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Perrin LH,Loche M,Dedet JP,Roussilhon C,Fandeur T

    更新日期:1984-04-01 00:00:00

  • Increased concentration of serum IgA antibody to pneumococcal polysaccharides in patients with IgA nephropathy.

    abstract::It has been postulated that IgA nephropathy (IgAGN) is caused by deposition within the glomerular mesangium of IgA polymers and IgA containing immune complexes which are overproduced in response to antigens presented at mucosal surfaces. To test this, the concentrations of specific antibodies to capsular polysaccharid...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Drew PA,Nieuwhof WN,Clarkson AR,Woodroffe AJ

    更新日期:1987-01-01 00:00:00

  • Effects of cyclosporin A on immunoglobulin production by EB virus stimulated lymphocytes.

    abstract::Cyclosporin A (Cy A) enhances immunoglobulin (Ig) production by EB virus stimulated peripheral blood lymphocytes from normal subjects with prior immunity to EB virus. In 11 normal adults, this enhancement of Ig production correlated with T cell-mediated cytotoxic regression. Cultures from normal healthy adults without...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Pereira RS,Gear AJ,Doré CJ,Webster AD

    更新日期:1983-07-01 00:00:00

  • Inhibition of human monocyte Fc receptor and HLA-DR antigen expression by pregnancy alpha-2 glycoprotein.

    abstract::The precise factor responsible for the altered immunological reactivity and diminished inflammatory responses occurring in pregnancy is unknown. Recent observations implicate the pregnancy-associated alpha-2 glycoprotein (PAG). Using rosette assays for the detection of cells carrying Fc receptors and for the demonstra...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Persellin RH,Rhodes J

    更新日期:1981-11-01 00:00:00

  • Actinobacillus actinomycetemcomitans Y4 capsular polysaccharide induces IL-1beta mRNA expression through the JNK pathway in differentiated THP-1 cells.

    abstract::Capsular polysaccharide from Actinobacillus actinomycetemcomitans Y4 (Y4 CP) induces bone resorption in a mouse organ culture system and osteoclast formation in mouse bone marrow cultures, as reported in previous studies. We also found that Y4 CP inhibits the release of interleukin (IL)-6 and IL-8 from human gingival ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Iwata T,Mitani A,Ishihara Y,Tanaka S,Yamamoto G,Kikuchi T,Naganawa T,Matsumura Y,Suga T,Koide M,Sobue T,Suzuki T,Noguchi T

    更新日期:2005-08-01 00:00:00

  • A human autoantibody to peroxisomes.

    abstract::A complement fixing, non-organ specific IgG autoantibody is described in 29 patients. The autoantibody gives a highly characteristic, granular staining of liver cells, proximal kidney tubules and stomach surface epithelium. By studies with various subcellular fractions from rat liver, employing two different technique...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Holm R,Gaarder PI,Helgeland L,Falkenhaug EI

    更新日期:1985-08-01 00:00:00