Desialylation of dying cells with catalytically active antibodies possessing sialidase activity facilitate their clearance by human macrophages.


:Recently we reported the first known incidence of antibodies possessing catalytic sialidase activity (sialidase abzymes) in the serum of patients with multiple myeloma and systemic lupus erythematosus (SLE). These antibodies desialylate biomolecules, such as glycoproteins, gangliosides and red blood cells. Desialylation of dying cells was demonstrated to facilitate apoptotic cell clearance. In this study we assessed the possibility to facilitate dying cell clearance with the use of F(ab)2 fragments of sialidase abzymes. Two sources of sialidase abzymes were used: (i) those isolated from sera of patients with SLE after preliminary screening of a cohort of patients for sialidase activity; and (ii) by creating an induced sialidase abzyme through immunization of a rabbit with synthetic hapten consisting of a non-hydrolysable analogue of sialidase reaction conjugated with bovine serum albumin (BSA) or keyhole limpet haemocyanin (KLH). Antibodies were purified by ammonium sulphate precipitation, protein-G affinity chromatography and size exclusion-high performance liquid chromatography (HPLC-SEC). Effect of desialylation on efferocytosis was studied using human polymorphonuclear leucocytes (PMN), both viable and aged, as prey, and human monocyte-derived macrophages (MoMa). Treatment of apoptotic and viable prey with both disease-associated (purified from blood serum of SLE patients) and immunization-induced (obtained by immunization of rabbits) sialidase abzymes, its F(ab)2 fragment and bacterial neuraminidase (as positive control) have significantly enhanced the clearance of prey by macrophages. We conclude that sialidase abzyme can serve as a protective agent in autoimmune patients and that artificial abzymes may be of potential therapeutic value.


Clin Exp Immunol


Tomin A,Dumych T,Tolstyak Y,Kril I,Mahorivska I,Bila E,Stoika R,Herrmann M,Kit Y,Bilyy R




Has Abstract


2015-01-01 00:00:00












  • DC-SIGN expression on podocytes and its role in inflammatory immune response of lupus nephritis.

    abstract::Podocytes, the main target of immune complex, participate actively in the development of glomerular injury as immune cells. Dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) is an innate immune molecular that has an immune recognition function, and is involved in mediation of ce...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Cai M,Zhou T,Wang X,Shang M,Zhang Y,Luo M,Xu C,Yuan W

    更新日期:2016-03-01 00:00:00

  • Identification of DNA-binding proteins on human umbilical vein endothelial cell plasma membrane.

    abstract::The binding of anti-DNA antibodies to the endothelial cell is mediated through DNA, which forms a bridge between the immunoglobulin and the plasma membrane. We have shown that 32P-labelled DNA bound to the plasma membrane of human umbilical vein endothelial cells (HUVEC) by a saturable process, which could be competit...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Chan TM,Frampton G,Cameron JS

    更新日期:1993-01-01 00:00:00

  • Prime-boost vaccination strategy with bacillus Calmette-Guérin (BCG) and liposomized alpha-crystalline protein 1 reinvigorates BCG potency.

    abstract::Bacillus Calmette-Guérin (BCG) remains the only available and most widely administered vaccine against Mycobacterium tuberculosis (Mtb), yet it fails to protect vaccinated individuals either from primary infection or reactivation of latent tuberculosis (TB). Despite BCG's variable efficacy against TB, the fact remains...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Siddiqui KF,Amir M,Khan N,Rama Krishna G,Sheikh JA,Rajagopal K,Agrewala JN

    更新日期:2015-08-01 00:00:00

  • Immunomodulatory effects of Lactobacillus plantarum colonizing the intestine of gnotobiotic rats.

    abstract::We have studied the effect of the probiotic strain Lactobacillus plantarum 299v on the immune functions of gnotobiotic rats. One group of germ-free rats was colonized with the type 1-fimbriated Escherichia coli O6:K13:H1 and another group with the same E. coli strain together with L. plantarum 299v. One and 5 weeks af...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Herías MV,Hessle C,Telemo E,Midtvedt T,Hanson LA,Wold AE

    更新日期:1999-05-01 00:00:00

  • Interferon-gamma and tumour necrosis factor-alpha production by CD4+ T and CD8+ T lymphocytes in AIDS patients with tuberculosis.

    abstract::Tuberculosis (TB) is usually more severe in HIV-infected patients, and the immune derangement found in co-infected patients may differ from that in each isolated disease. Following mitogen stimulation of peripheral blood mononuclear cells (PBMC), interferon (IFN)-gamma and tumour necrosis factor (TNF)-alpha production...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: de Castro Cunha RM,Kallas EG,Rodrigues DS,Nascimento Burattini M,Salomao R

    更新日期:2005-06-01 00:00:00

  • Busulfan and chloramphenicol induced T cell lymphoma: cell surface characteristics and functional properties.

    abstract::We report the immunological studies on three transplantable lymphoma lines that developed when CAF1 mice were injected with busulfan and chloramphenicol. The lymphoma cells displayed Thy-1.2, brain associated antigen, and H-2d alloantigen. They were negative for surface IgM and Ia antigens. Expression of T cell differ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Bhoopalam N,Price K,Norgello H,Barone-Varelas J,Fried W

    更新日期:1986-06-01 00:00:00

  • Early deficit of lymphocytes in Wiskott-Aldrich syndrome: possible role of WASP in human lymphocyte maturation.

    abstract::Wiskott-Aldrich syndrome (WAS) is an X-linked platelet/immunodeficiency disease. The affected gene encodes WASP, a multidomain protein that regulates cytoskeletal assembly in blood cells. Patients have recurring infections, and their lymphocytes exhibit deficient proliferative responses in vitro. We report an evaluati...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Park JY,Kob M,Prodeus AP,Rosen FS,Shcherbina A,Remold-O'Donnell E

    更新日期:2004-04-01 00:00:00

  • Idiopathic histaminergic angioedema without wheals: a case series of 31 patients.

    abstract::Idiopathic histaminergic acquired angioedema (IH-AAE) is a common cause of recurrent angioedema without wheals. It is a mast cell-mediated disease thought to belong to the same clinical entity as chronic urticaria (CU). The objective of this study was to describe the clinical and epidemiological characteristics of IH-...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Faisant C,Boccon-Gibod I,Mansard C,Dumestre Perard C,Pralong P,Chatain C,Deroux A,Bouillet L

    更新日期:2016-07-01 00:00:00

  • Long-term serological evaluation of patients with cystic echinococcosis treated with benzimidazole carbamates.

    abstract::Seeking better immunological markers indicating the long-term outcome of cystic echinococcosis (CE) after chemotherapy we studied 23 patients receiving albendazole, clinically followed for 8 years, and grouped ultrasonographically according to therapeutic outcome. Antibody responses against a partially purified fracti...

    journal_title:Clinical and experimental immunology

    pub_type: 临床试验,杂志文章


    authors: Riganò R,Ioppolo S,Ortona E,Margutti P,Profumo E,Ali MD,Di Vico B,Teggi A,Siracusano A

    更新日期:2002-09-01 00:00:00

  • A restricted T cell response to myelin basic protein (MBP) is stable in multiple sclerosis (MS) patients.

    abstract::The close resemblance of MS to the animal model experimental autoimmune encephalomyelitis (EAE) has provided compelling data sustaining a pathogenic role of circulating T cells reactive against MBP. T cell antigen receptor (TCR) usage in EAE is commonly considered restricted; nevertheless, dynamic changes of TCR usage...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Uccelli A,Giunti D,Salvetti M,Ristori G,Fenoglio D,Abbruzzese,Mancardi G

    更新日期:1998-01-01 00:00:00

  • Circulating immune complexes in six patients with trichinosis.

    abstract::The sera of six patients infected with Trichinella spiralis were studied for the presence of circulating immune complexes (CIC). CIC were present in all six patients studied 1 month after infection. In two patients in whom serial serum samples were available, as clinical improvement occurred there was a decrease in th...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Géniteau M,Verroust PJ,Smith MD,Morel-Maroger L,Saimot G,Coulaud JP

    更新日期:1977-10-01 00:00:00

  • Autoantibodies to intermediate filaments in sera of patients with Schistosoma mansoni infection.

    abstract::Autoantibodies to the intermediate filament proteins vimentin and keratin were studied in sera of 50 Caribbean patients with Schistosoma mansoni infection and 50 control subjects. Autoantibodies were detected by indirect immunofluorescence on HEp-2 cells pretreated with colchicine. The incidence of anti-vimentin antib...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Boehme MW,Kataaha PK,Holborow EJ

    更新日期:1989-08-01 00:00:00

  • Specific stimulation of peripheral blood mononuclear cells from patients with acute myocarditis by peptide-bound flavin adenine dinucleotide (FAD), a naturally occurring autologous hapten.

    abstract::The tryptic FAD-peptide carrying the flavin in 8alpha-(N3)histidyl linkage as natural hapten was isolated by HPLC from the bacterial enzyme 6-hydroxy-d-nicotine oxidase. The same flavin protein linkage is found in the mitochondrial succinate dehydrogenase flavoprotein subunit, the predominant flavoprotein with covalen...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Cicek G,Schiltz E,Staiger J,Neumann FJ,Melchers I,Brandsch R

    更新日期:2003-05-01 00:00:00

  • Immunology of membranous nephropathy: from animal models to humans.

    abstract::Membranous nephropathy (MN), the leading cause of nephrotic syndrome in adults, is characterized by the deposition of subepithelial immune deposits that consist mainly of immunoglobulin (Ig)G and complement. Most of the cases are primary or idiopathic (iMN), while only approximately 25% of the cases are secondary to s...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Sinico RA,Mezzina N,Trezzi B,Ghiggeri GM,Radice A

    更新日期:2016-02-01 00:00:00

  • Gammadelta+ and CD4+ alphabeta+ human T cell subset responses upon stimulation with various Mycobacterium tuberculosis soluble extracts.

    abstract::By using a flow cytometric technique which allows direct identification of proliferating cells within mixed cell populations, we have previously described that soluble extracts obtained from Mycobacterium tuberculosis or M. avium represent strong stimuli for human gammadelta+ T cells. In the present study, we demonstr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Batoni G,Esin S,Harris RA,Källenius G,Svenson SB,Andersson R,Campa M,Wigzell H

    更新日期:1998-04-01 00:00:00

  • Study on the nature of the Goodpasture antigen using a basement membrane-producing mouse tumour.

    abstract::Autoantibodies in the sera of patients with Goodpasture's syndrome showed a strong reaction in indirect immunofluorescence tests on unfixed, frozen sections of a mouse tumour (EHS sarcoma), previously shown to produce extracellular basement membrane. Anti-basement membrane antibodies from patients with bullous pemphig...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Wick G,Timpl R

    更新日期:1980-03-01 00:00:00

  • Dysregulation of B7.2 (CD86) expression on monocytes of HIV-infected individuals is associated with altered production of IL-2.

    abstract::T helper (Th) responses are mediated in part by immunoregulatory cytokines and the signals delivered by the costimulatory CD28-B7 pathway. In this study, we have investigated the relationship between the regulation of B7 isoform expression on antigen-presenting cells from HIV+ individuals and the production of Th cyto...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kumar A,Angel JB,Aucoin S,Creery WD,Daftarian MP,Cameron DW,Filion L,Diaz-Mitoma F

    更新日期:1999-07-01 00:00:00

  • Influence of the mouse Bcg, Tbc-1 and xid genes on resistance and immune responses to tuberculosis infection and efficacy of bacille Calmette-Guérin (BCG) vaccination.

    abstract::We have studied the role of three mouse distinct non-H-2 genes (Bcg, Tbc-1, xid) in several phenomena of antituberculosis immunity and resistance. On the basis of median survival time (MST) of mice following infection with virulent Mycobacterium tuberculosis H37Rv, Bcg gene did not control resistance to the lethal dos...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Nikonenko BV,Apt AS,Mezhlumova MB,Avdienko VG,Yeremeev VV,Moroz AM

    更新日期:1996-04-01 00:00:00

  • Calcium efflux and influx in f-met-leu-phe (fMLP)-activated human neutrophils are chronologically distinct events.

    abstract::The kinetics of efflux of calcium mobilized from intracellular stores following activation of human neutrophils with the synthetic chemotactic tripeptide, fMLP (1 microM), as well as that of the subsequent store-operated influx of this cation, has been measured by radiometric procedures using 45Ca. These procedures en...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Anderson R,Goolam Mahomed A

    更新日期:1997-10-01 00:00:00

  • The immune response to phichi174 in man. IV. Primary and secondary antibody production in patients with chronic lymphatic leukaemia.

    abstract::Ten patients with chronic lymphatic leukaemia (CLL) were injected with the antigen phichi174 intravenously on two occasions to test their capacity for antibody production. Nine out of ten patients failed to develop a primary response, and five out of ten failed to develop a secondary response. Only one out of the ten ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Hamblin TJ,Jones JV,Peacock DB

    更新日期:1975-07-01 00:00:00

  • Prospective comparison of two enzyme-linked immunosorbent spot assays for the diagnosis of Lyme neuroborreliosis.

    abstract::Commercial cellular tests are used to diagnose Lyme borreliosis (LB), but studies on their clinical validation are lacking. This study evaluated the utility of an in-house and a commercial enzyme-linked immunosorbent spot (ELISpot) assay for the diagnosis of Lyme neuroborreliosis (LNB). Prospectively, peripheral blood...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: van Gorkom T,Voet W,Sankatsing SUC,Nijhuis CDM,Ter Haak E,Kremer K,Thijsen SFT

    更新日期:2020-03-01 00:00:00

  • Epitope mapping of an HLA-B27 monoclonal antibody that also reacts with a 35-kD bacterial outer-membrane protein.

    abstract::Ye-3 is an HLA-B27-specific murine monoclonal antibody recognizing the heat-modifiable protein of Enterobacteriaceae. Here we used recombinant hybrid molecules between the HLA-B7 and HLA-B27 antigens to delineate the epitope recognized by Ye-3. Results of these experiments indicated that the segment of HLA-B*2705 span...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Toubert A,Hamachi M,Raffoux C,Park MS,Yu DT

    更新日期:1990-10-01 00:00:00

  • Increased secretion of IL-18 in vitro by peripheral blood mononuclear cells of patients with bronchial asthma and atopic dermatitis.

    abstract::This study was performed to determine whether or not IL-18, formerly called IFN-gamma-inducing factor, is involved in the pathogeneses of allergic disorders. Peripheral blood mononuclear cells (PBMC) were obtained from patients with allergic bronchial asthma (BA), patients with atopic dermatitis (AD) and controls who ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: El-Mezzein RE,Matsumoto T,Nomiyama H,Miike T

    更新日期:2001-11-01 00:00:00

  • Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells.

    abstract::Intraepithelial lymphocytes (IELs) can be identified among epithelial cells in systemic mucosal tissues. Although intestinal IELs play a crucial role in mucosal immunity, their bronchial counterparts have not been well studied. The purpose of this study was to determine the immunological functions of human bronchial I...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Hirosako S,Goto E,Fujii K,Tsumori K,Hirata N,Tsumura S,Kamohara H,Kohrogi H

    更新日期:2009-02-01 00:00:00

  • Function of C3 in a humoral response: iC3b/C3dg bound to an immune complex generated with natural antibody and a primary antigen promotes antigen uptake and the expression of co-stimulatory molecules by all B cells, but only stimulates immunoglobulin synt

    abstract::Previous studies have shown that an optimal humoral response to a primary protein antigen requires C3 and CR2 (CD21). Sera from non-immunized donors contain natural IgM and IgG antibodies to the primary antigen keyhole limpet haemocyanin (KLH), and these have been previously shown to form immune complexes (IC) that ac...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Thornton BP,Vetvicka V,Ross GD

    更新日期:1996-06-01 00:00:00

  • Induction and increase of HLA-DR antigen expression by immune interferon on ML-3 cell line enhances the anti-HLA-DR immunotoxin activity.

    abstract::In order to evaluate the impact of induction and increase target antigen expression on immunotoxin potency, we measured the potentiating effect of recombinant immune interferon-gamma (rIFN-gamma) on the cytotoxicity of an anti HLA-DR ricin A-chain immunotoxin (2G5 RTA-IT) on the myeloid cell line ML-3. After 48 h of i...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Chiron M,Jaffrezou JP,Carayon P,Bordier C,Roubinet F,Xavier C,Brandely M,Laurent G

    更新日期:1990-11-01 00:00:00

  • NK cells, displaying early activation, cytotoxicity and adhesion molecules, are associated with mild dengue disease.

    abstract::During the innate immune response against infections, Natural Killer (NK) cells are as important effector cells as are Cytotoxic T lymphocytes (CTL) generated after antigenic stimulation in the adaptative response. NK cells increase in numbers, after viral infection or vaccination. We investigated the NK cell and CD8 ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Azeredo EL,De Oliveira-Pinto LM,Zagne SM,Cerqueira DI,Nogueira RM,Kubelka CF

    更新日期:2006-02-01 00:00:00

  • Respiratory syncytial virus-induced cytokine production by neonatal macrophages.

    abstract::The induction of immunoregulatory cytokines IL-1 beta, IL-6, IL-12, tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) was studied with neonatal (cord blood) monocyte-derived macrophages (MDM) after in vitro infection with respiratory syncytial virus (RSV). The expression of mRNAs for these cyto...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Tsutsumi H,Matsuda K,Sone S,Takeuchi R,Chiba S

    更新日期:1996-12-01 00:00:00

  • Imatinib inhibits CSF1R that stimulates proliferation of rheumatoid arthritis fibroblast-like synoviocytes.

    abstract::In this study, we aimed to explore the effects of imatinib on the proliferation of rheumatoid arthritis synovial cell (RA-FLS) and inflammatory responses by regulating CSF1R. Differential genes were screened via microarray analysis, followed by being analysed through the weighted co-expression network (WGCNA) network,...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Hu X,Tang J,Hu X,Bao P,Pan J,Ou Y,Deng W,Liang Y

    更新日期:2019-02-01 00:00:00

  • Cellular immune responses against hepatitis C virus: the evidence base 2002.

    abstract::Hepatitis C virus (HCV) is an RNA virus which is estimated to persistently infect about 170 million people worldwide. After acute infection, there is an initial period during which long-term outcome is decided. There is strong evidence that the cellular immune responses, involving both CD4+ and CD8+ T lymphocytes, are...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Ward S,Lauer G,Isba R,Walker B,Klenerman P

    更新日期:2002-05-01 00:00:00