Monocytes show immunoregulatory capacity on CD4+ T cells in a human in-vitro model of extracorporeal photopheresis.

Abstract:

:Extracorporeal photopheresis (ECP) is a widely used immunomodulatory therapy for the treatment of various T cell-mediated disorders such as cutaneous T cell lymphoma (CTCL), graft-versus-host disease (GvHD) or systemic sclerosis. Although clinical benefits of ECP are already well described, the underlying mechanism of action of ECP is not yet fully understood. Knowledge on the fate of CD14+ monocytes in the context of ECP is particularly limited and controversial. Here, we investigated the immunoregulatory function of ECP treated monocytes on T cells in an in-vitro ECP model. We show that ECP-treated monocytes significantly induce proinflammatory T cell types in co-cultured T cells, while anti-inflammatory T cells remain unaffected. Furthermore, we found significantly reduced proliferation rates of T cells after co-culture with ECP-treated monocytes. Both changes in interleukin secretion and proliferation were dependent on cell-contact between monocytes and T cells. Interestingly, blocking interactions of programmed death ligand 1 (PD-L1) to programmed death 1 (PD-1) in the in-vitro model led to a significant recovery of T cell proliferation. These results set the base for further studies on the mechanism of ECP, especially the regulatory role of ECP-treated monocytes.

journal_name

Clin Exp Immunol

authors

Wiese F,Reinhardt-Heller K,Volz M,Gille C,Köstlin N,Billing H,Handgretinger R,Holzer U

doi

10.1111/cei.13232

subject

Has Abstract

pub_date

2019-03-01 00:00:00

pages

369-380

issue

3

eissn

0009-9104

issn

1365-2249

journal_volume

195

pub_type

杂志文章
  • Clinical significance of serum soluble IL-2R levels in patients with adult T cell leukaemia (ATL) and HTLV-1 carriers.

    abstract::The levels of soluble IL-2Ralpha (sIL-2Ralpha) in serum were measured in HTLV-1 carriers and ATL patients in order to evaluate their possible correlation with clinical status. Mean sIL-2R levels in ATL patients were found to be 9704 U/ml for the acute/lymphoma type, 1961 U/ml for the chronic type and 788 U/ml for the ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2249.2000.01136.x

    authors: Araki K,Harada K,Nakamoto K,Shiroma M,Miyakuni T

    更新日期:2000-02-01 00:00:00

  • HIV isolation from pulmonary cells derived from bronchoalveolar lavage.

    abstract::In a study of 75 alveolar cell co-cultures from 55 HIV-seropositive subjects, p24 antigen production was identified by ELISA in 11 out of 26 (42%) of unseparated cell cultures, 15 out of 39 (38%) purified alveolar macrophage cultures and eight out of 10 purified alveolar lymphocyte samples. Positivity in unseparated c...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Jeffrey AA,Israël-Biet D,Andrieu JM,Even P,Venet A

    更新日期:1991-06-01 00:00:00

  • Specificity and immunochemical properties of antibodies to bacterial DNA in sera of normal human subjects and patients with systemic lupus erythematosus (SLE).

    abstract::To elucidate the mechanisms of anti-DNA production, we assessed the binding of sera of normal human subjects (NHS) and patients with SLE to a panel of bacterial and mammalian DNA. Using single-stranded DNA as antigens in an ELISA, NHS showed significant binding to some but not all bacterial DNA, while lacking reactivi...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2249.1997.4301328.x

    authors: Wu ZQ,Drayton D,Pisetsky DS

    更新日期:1997-07-01 00:00:00

  • Heterogeneity of stem cells in severe combined immunodeficiency.

    abstract::Two patients with severe combined immunodeficiency disease (SCID) having variable B-cell development have been shown to have marrow precursors of lymphoid cells which can be induced in vitro by thymic factors to express certain T-cell surface characteristics (HTLA+ phenotypes). Their marrow cells could not, however, b...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Incefy GS,Grimes E,Kagan WA,Goldstein G,Smithwick E,O'Reilly R,Good RA

    更新日期:1976-09-01 00:00:00

  • Anaphylatoxins inhibit C2 production.

    abstract::Anaphylatoxins C5a and C3a and their des Arg derivatives inhibited C2 production by mononuclear phagocytes. C5a and C5adesArg which were approximately equipotent (IC50 = 10(-10) mol/l) were more effective than C3a (IC50 = 5 X 10(-8) mol/l) which was approximately 10-20-fold more potent than C3adesArg IC50 = 5 X 10(-6)...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Lappin D,Damerau B,Whaley K

    更新日期:1983-11-01 00:00:00

  • Activation of human peripheral blood lymphocytes by a trypanosome-derived mitogen.

    abstract::Activation of human peripheral blood lymphocytes from unsensitized donors by fractions of T.b rhodesiense has been demonstrated in vitro. Both T and B cells were stimulated to differentiate into blast cells. Purified T cells failed to proliferate, but did so when mitomycin C-treated non-T cells were added. Non-T cell ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Selkirk ME,Ogilvie BM,Platts-Mills TA

    更新日期:1981-09-01 00:00:00

  • Abnormal liver function in common variable immunodeficiency disorders due to nodular regenerative hyperplasia.

    abstract::Patients with common variable immunodeficiency disorders are monitored for liver function test abnormalities. A proportion of patients develop deranged liver function and some also develop hepatomegaly. We investigated the prevalence of abnormalities and types of liver disease, aiming to identify those at risk and det...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.2008.03711.x

    authors: Ward C,Lucas M,Piris J,Collier J,Chapel H

    更新日期:2008-09-01 00:00:00

  • Failure of Mycobacterium leprae soluble antigens to suppress delayed-type hypersensitivity reaction to tuberculin.

    abstract::In order to test a published claim that the inclusion of Mycobacterium leprae antigens with a tuberculin skin test reagent can suppress delayed-type hypersensitivity (DTH) to tuberculin in both paucibacillary and multibacillary leprosy cases, 109 leprosy cases and 104 non-leprosy controls were skin-tested simultaneous...

    journal_title:Clinical and experimental immunology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:

    authors: Fine PE,Gruer PJ,Maine N,Ponnighaus JM,Rees RJ,Stanford JL

    更新日期:1989-08-01 00:00:00

  • Analysis of regulatory T cell associated forkhead box P3 expression in the lungs of patients with sarcoidosis.

    abstract::In pulmonary sarcoidosis, the typical T helper 1-mediated immune response in the lungs has been proposed to be co-ordinated by regulatory T cells; however, their exact role needs to be clarified. We used real-time polymerase chain reaction to study genes involved in regulatory T cell functions in CD4+ T cells isolated...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.2008.03609.x

    authors: Idali F,Wahlström J,Müller-Suur C,Eklund A,Grunewald J

    更新日期:2008-04-01 00:00:00

  • Production of interleukin (IL)-5 and IL-10 accompanies T helper cell type 1 (Th1) cytokine responses to a major thyroid self-antigen, thyroglobulin, in health and autoimmune thyroid disease.

    abstract::Tumour necrosis factor (TNF)-alpha and interferon (IFN)-gamma exert detrimental effects in organ-specific autoimmune disease, while both destructive and protective roles have been demonstrated for interleukin (IL)-10, IL-4 and IL-5. We examined the production of these cytokines by peripheral blood mononuclear cells (P...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.2006.03283.x

    authors: Nielsen CH,Hegedüs L,Rieneck K,Moeller AC,Leslie RG,Bendtzen K

    更新日期:2007-02-01 00:00:00

  • A high level of serum neopterin is associated with rapidly progressive interstitial lung disease and reduced survival in dermatomyositis.

    abstract::Neopterin is primarily synthesized and released by activated macrophages/monocytes upon stimulation with interferon-γ and is considered as a marker for macrophage activation. This study aimed to analyze the serum levels of neopterin in patients with dermatomyositis (DM) in association with clinical manifestations, lab...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/cei.13404

    authors: Peng QL,Zhang YM,Liang L,Liu X,Ye LF,Yang HB,Zhang L,Shu XM,Lu X,Wang GC

    更新日期:2020-03-01 00:00:00

  • Immunoglobulin replacement therapy by self-infusion at home.

    abstract::Twelve patients, ten with common variable hypogammaglobulinaemia and two with hypogammaglobulinaemia secondary to chronic lymphocytic leukaemia (CLL), have been taught to self-infuse their intravenous immunoglobulin replacement therapy. Follow-up of these patients has shown that regular self-infusion at home is feasib...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Chapel H,Brennan V,Delson E

    更新日期:1988-07-01 00:00:00

  • Generation and functional analysis of T cell lines and clones specific for schistosomula released products (SRP-A).

    abstract::Antigens present in the products released by the larval stage of schistosome (SRP-A) were shown to induce a strong cytotoxic and protective IgE response both in the rat and the monkey. T cell lines and clones specific for SRP-A or 26 kD antigens which are the main target of the cytotoxic IgE have been derived. The pas...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Damonneville M,Velge F,Verwaerde C,Pestel J,Auriault C,Capron A

    更新日期:1987-08-01 00:00:00

  • Generation of monoclonal anti-dermatan sulphate antibodies cross-reacting to calf thymus DNA.

    abstract::Monoclonal anti-dermatan sulphate antibodies were produced with hybridization after immunizing BALB/c mice with dermatan sulphate (DS). These antibodies were tested for cross-reactivity to double-stranded DNA (dsDNA) and heat-denatured DNA (ssDNA). Five clones had binding activity to DS, dsDNA and ssDNA in enzyme-link...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Ohnishi K,Sagawa A,Nakabayashi T,Ohmoto A,Megumi,Yoshikawa M,Watanabe I,Mukai M,Yasuda I,Nakagawa S

    更新日期:1989-01-01 00:00:00

  • A systems medicine approach reveals disordered immune system and lipid metabolism in multiple sclerosis patients.

    abstract::Identification of autoimmune processes and introduction of new autoantigens involved in the pathogenesis of multiple sclerosis (MS) can be helpful in the design of new drugs to prevent unresponsiveness and side effects in patients. To find significant changes, we evaluated the autoantibody repertoires in newly diagnos...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/cei.13087

    authors: Pazhouhandeh M,Sahraian MA,Siadat SD,Fateh A,Vaziri F,Tabrizi F,Ajorloo F,Arshadi AK,Fatemi E,Piri Gavgani S,Mahboudi F,Rahimi Jamnani F

    更新日期:2018-04-01 00:00:00

  • Monocyte IgG-Fc receptors in myotonic dystrophy.

    abstract::Myotonic dystrophy (MyD), an autosomal dominant neuromuscular disease with multisystem abnormalities, is associated with hypercatabolism of IgG. The hypercatabolism is not related to structural abnormalities of the IgG molecule in MyD but appears to be due to a derangement of the serum IgG concentration-fractional cat...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Banerjee D,McClintock J,Silver MM,Hudson AJ

    更新日期:1982-12-01 00:00:00

  • An altered repertoire of T cell receptor V gene expression by rheumatoid synovial fluid T lymphocytes.

    abstract::The pattern of T cell receptor V gene expression by lymphocytes from rheumatoid synovial fluid and paired peripheral blood samples was compared using a polymerase chain reaction (PCR)-based assay. Eight rheumatoid arthritis (RA) patients who had varying durations of disease (from 2 to 20 years) were studied. In all pa...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1992.tb05865.x

    authors: Lunardi C,Marguerie C,So AK

    更新日期:1992-12-01 00:00:00

  • CD5-positive B cells after T cell depleted bone marrow transplantation.

    abstract::CD5 is an antigen normally found on T cells and on a minority subpopulation of B cells in fetal spleen and tonsil and on the majority of cells in B-chronic lymphocytic leukaemia. Recent reports described the occurrence of large numbers of CD5-positive B cells in the peripheral blood after bone marrow transplantation (...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Drexler HG,Brenner MK,Wimperis JZ,Gignac SM,Janossy G,Prentice HG,Hoffbrand AV

    更新日期:1987-06-01 00:00:00

  • Prognostic value of serum immunoglobulin A antibodies to pol gene products during HIV-1 infection.

    abstract::Serum specimens from 66 HIV-1-infected subjects were tested by ELISA for the presence of IgA antibodies to HIV-1: 44 samples were found positive and 37 were confirmed by immunoblot. In these subjects, the presence of anti-HIV IgA antibodies was studied in relation to the total count of circulating CD4+ lymphocytes and...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1993.tb05889.x

    authors: Quesnel A,Pozzetto B,Moja P,Grattard F,Lucht FR,Touraine JL,Gaudin OG,Genin C

    更新日期:1993-02-01 00:00:00

  • The distribution of a specific HLA-B27-associated cell surface component on the tissues of patients with ankylosing spondylitis.

    abstract::In this paper, we report the presence on Epstein-Barr virus-transformed lymphoblastoid cell lines on platelets and on fibroblasts of an HLA-B27-associated cell surface complex (antigenically related to some antigens of Klebsiella K43 and K21) which is identical to or cross-reactive with the determinant present on the ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Alexander K,Edwards C,Misko IS,Geczy AF,Bashir HV,Edmonds JP

    更新日期:1981-07-01 00:00:00

  • IL-1 receptor antagonist (IL-1Ra) does not inhibit the production of C-reactive protein or serum amyloid A protein by human primary hepatocytes. Differential regulation in normal and tumour cells.

    abstract::The synthesis of some class 1 acute-phase proteins (APP), including C-reactive protein (CRP) and serum amyloid A (SAA) protein is completely blocked by the IL-1 receptor antagonist (IL-1Ra), whereas the production of fibrinogen, a class 2 APP, is increased by IL-1Ra in hepatoma cells, but this has never been tested in...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1995.tb03669.x

    authors: Gabay C,Genin B,Mentha G,Iynedjian PB,Roux-Lombard P,Guerne PA

    更新日期:1995-05-01 00:00:00

  • Antibody-directed complement-mediated cytotoxicity to hepatocytes from patients with chronic hepatitis B.

    abstract::The susceptibility of hepatocytes from patients with chronic hepatitis B to complement-dependent cytotoxicity mediated by heterologous antibodies to hepatitis B virus core (anti-HBc) and surface (anti-HBs) antigens and to hepatic asialoglycoprotein receptor was examined using a microcytotoxicity assay. The anti-HBc-in...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1995.tb03658.x

    authors: Michalak TI,Lau JY,McFarlane BM,Alexander GJ,Eddleston AL,Williams R

    更新日期:1995-05-01 00:00:00

  • Herpes gestationis: studies on the binding characteristics, activity and pathogenetic significance of the complement-fixing factor.

    abstract::The sera of seven out of eight patients with Herpes gestationis contained a factor which fixed C3 but not detectable amounts of immunoglobulin (Ig) on the basement membrane zone (BMZ) of normal human skin. Using C2-deficient human serum and C4-deficient guinea-pig serum, fixation was shown to be dependent upon activat...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Carruthers JA,Ewins AR

    更新日期:1978-01-01 00:00:00

  • Age-related autoantibody production in a nonhuman primate model.

    abstract::Autoantibody production increases with ageing. However, the pathological significance of this increase as well as the corresponding underlying mechanisms are poorly understood. To further our understanding of the role that ageing plays in the development of autoantibody responses, we used a novel nonhuman primate mode...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2249.2001.01454.x

    authors: Attanasio R,Brasky KM,Robbins SH,Jayashankar L,Nash RJ,Butler TM

    更新日期:2001-03-01 00:00:00

  • Virus-specific IgA in serum, saliva, and tears of children with measles.

    abstract::Measles-specific IgA antibody titres were determined by radioimmunoassay (RIA) for serial serum, saliva and tear samples obtained from 21 children with measles infection, from onset of rash until up to 14 months later. Serum IgA titres rose rapidly after onset of illness and remained detectable throughout the follow-u...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Friedman MG,Phillip M,Dagan R

    更新日期:1989-01-01 00:00:00

  • T cell subsets in leprosy lesions: in situ characterization using monoclonal antibodies.

    abstract::Cryostat sections of dermal lesions from 30 untreated leprosy patients were studied by indirect immunofluorescence using monoclonal antibodies defining T cell subsets and Ia like antigens. Most lymphocytes in leprosy lesions were positive for OKT3 and Ia like antigens indicating thereby the presence of activated T cel...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Narayanan RB,Bhutani LK,Sharma AK,Nath I

    更新日期:1983-03-01 00:00:00

  • Influence of the Ity/Lsh/Bcg gene on the development of suppressor cell precursors in the early phase of the infection of mice with Mycobacterium lepraemurium.

    abstract::In vitro inducible suppressor cell precursors were detected in the spleen of BALB/c but not in DBA/2 mice infected intraperitoneally with 10(8) Mycobacterium lepraemurium bacilli, thus suggesting that their development is genetically controlled. Two pairs of mouse strains congenic at the Ity/Lsh/Bcg locus (BALB/c-C.D2...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.1994.tb06545.x

    authors: Gosselin D,Turcotte R,Lemieux S

    更新日期:1994-05-01 00:00:00

  • Iron down-regulates macrophage anti-tumour activity by blocking nitric oxide production.

    abstract::Although the inhibitory effect of iron on macrophage production of tumoricidal free radical nitric oxide (NO) has been reported, its possible influence on macrophage anti-tumour activity has not been established. In the present study, FeSO4 markedly reduced IFN-gamma + LPS-induced NO synthesis in mouse and rat macroph...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2249.2004.02515.x

    authors: Harhaji L,Vuckovic O,Miljkovic D,Stosic-Grujicic S,Trajkovic V

    更新日期:2004-07-01 00:00:00

  • Mice lacking the multidrug resistance protein 1 have a transiently impaired immune response during tuberculosis.

    abstract::A T helper (Th) 1 immune response is important for host defense against tuberculosis. The multidrug resistance protein (Mrp) 1 is constitutively present at low levels on Th2 lymphocytes, and is expressed on Th1 lymphocytes upon activation. To determine the role of Mrp1 in the pathogenesis of tuberculosis, Mrp1 deficie...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2249.2002.01952.x

    authors: Verbon A,Leemans JC,Weijer S,Florquin S,Van Der Poll T

    更新日期:2002-10-01 00:00:00

  • Immunoglobulin G subclass antibody responses in influenza A and parainfluenza type 1 virus infections.

    abstract::Antibody responses in immunoglobulin G1, G2, G3, G4, A (IgA1) and M isotypes were studied in 10 patients with an acute influenza A and in another 10 patients with a parainfluenza type 1 virus infection using radioimmunoassay with standardized monoclonal anti-immunoglobulins. A four-fold or greater increase of antibody...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章

    doi:

    authors: Julkunen I,Hovi T,Seppälä I,Mäkelä O

    更新日期:1985-04-01 00:00:00