Abstract：
:Calf sub-mandibular lymph node lymphocytes and human peripheral blood lymphocytes were oxidized by NaIO4. In liquid cell cultures, both types of cells were transformed with enhanced DNA synthesis. In mixed lymphocyte cultures, untreated human lymphocytes were transformed by irradiated-oxidized human cells. However, untreated calf lymphocytes were not transformed by irradiated-oxidized calf cells. Either human or calf lymphocytes were isolated from cell-cell interaction in agarose on slides. After 2-3 days of culture, cells were pulsed with tritiated thymidine. Autoradiography indicated greater numbers of cells with active DNA synthesis in NaIO4-oxidized calf cells and in calf cells incubated with Phaseolus vulgaris E-PHA compared to untreated lymphocytes. Similarly treated human cells did not show any increase in the numbers of cells with active DNA synthesis. No enhancement of DNA synthesis was seen in oxidized calf cells which were reduced with NaBH4 or cultured with hydroxyurea. In these restricted in vitro systems, calf cells are 'transformed' independent of cell-cell interaction, while human cells are not.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Presant CA,Parker Ssubject
Has Abstractpub_date
1977-01-01 00:00:00pages
183-9issue
1eissn
0009-9104issn
1365-2249journal_volume
27pub_type
杂志文章abstract：:Significant levels of EAC-rosette inhibition compared to control subjects were found in the sera of patients with focal and segmental hyalinosis (FSH), membranoproliferative glomerulonephritis (MPGN) and extra-membranous glomerulonephritis (EGN). In patients with IgA disease, although some sera produced high levels of...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-12-01 00:00:00
abstract：:A solid-phase platelet membrane enzyme linked immunosorbent assay (ELISA) was developed for the detection of circulating platelet binding IgG in serum. 18 out of 28 (64%) sera from patients with chronic idiopathic thrombocytopenic purpura (ITP) and 10 out of 21 (48%) sera from patients with acute ITP contained more pl...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：:One hundred and sixty-six unrelated children and adolescents having growth-onset, insulin-dependent diabetes mellitus (IDD) were studied for pancreatic-cytoplasmic islet cell antibodies (ICA) at various times from the diagnosis of the disease. A strong association between HLA antigens A9 and Bw16 and an absence of ICA...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-04-01 00:00:00
abstract：:The controlled induction of haemoxygenase-1 (HO-1), an enzyme that catabolizes haem, has been shown to reduce haem, preventing pathologies associated with haem toxicity. The hemoglobin genotype HbAS confers reduced susceptibility to severe complications of malaria by a mechanism that is not well understood. Using a lo...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12936
更新日期：2017-05-01 00:00:00
abstract：:The major subtypes of anti-neutrophil cytoplasmic antibodies (ANCA) detected by indirect immunofluorescence assay (IFA) are P-ANCA and C-ANCA. In patients with vasculitis, myeloperoxidase (MPO) is the major P-ANCA antigen and proteinase 3 (PR3) is the major C-ANCA antigen. BPI and azurocidin, which are also called 57-...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1996.d01-738.x
更新日期：1996-07-01 00:00:00
abstract：:Mercuric chloride (HgCl2)-induced autoimmunity in Brown Norway (BN) rats is a highly polarized polyclonal Th2-driven autoimmune response with increased IgE production, lymphoproliferation, vasculitis and proteinuria. The increase in serum IgE concentration is clearly measurable by day 4 after the first HgCl2 injection...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2002.01928.x
更新日期：2002-09-01 00:00:00
abstract：:In order to delineate the molecular pathogenesis of the increased susceptibility to CMV disease in HIV infection, the patterns of antigen responsiveness in HIV-infected and non-infected individuals were investigated. CMV was fractionated by SDS-PAGE and electroblotted onto nitrocellulose. Lymphoproliferative responses...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1990.tb05490.x
更新日期：1990-12-01 00:00:00
abstract：:An expanded T cell subpopulation (CD8+ Leu-7+) has previously been reported in the peripheral blood of patients with Crohn's disease. This subpopulation of T cells was associated with a 'covert suppressor' function, particularly in patients with mild/early Crohn's disease, suppressing immunoglobulin production in vitr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1990.tb03298.x
更新日期：1990-06-01 00:00:00
abstract：:The effects of 2.5-40 mg/kg nandrolone decanoate (ND) on the development and growth of mononuclear cell infiltrations in the submandibular glands of NZB/W (B/W) mice have been studied using a quantitative histological method. Injections with ND for 9 months, once every 3 weeks, starting at 4 weeks of age, reduced the ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:We studied the relationship between the HLA specificities associated with multiple sclerosis (MS) susceptibility in southern Italy and the reactivity of the human myelin basic protein (hMBP) immunogenic peptides 84-98 and 143-168, using short-term T-cell lines established from 9 MS patients and from 8 healthy individu...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2001.01457.x
更新日期：2001-02-01 00:00:00
abstract：:In this second review on chemokines, we focus on the polymorphisms and alternative splicings and on their consequences in disease. Because chemokines are key mediators in the pathogenesis of inflammatory, autoimmune, vascular and neoplastic disorders, a large number of studies attempting to relate particular polymorph...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2249.2007.03489.x
更新日期：2007-10-01 00:00:00
abstract：:Changes were assessed in lymphocyte sub-populations in various stages of human pregnancy. The percentage and absolute number of E-RFC decreased during pregnancy. There was a concomitant rise in the percentages of EAC-RFC and cells bearing SmIg with little change in their absolute numbers. EAC-RFC continued to rise pos...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:Increased serum cytokine levels have been reported in patients with autoimmune thyroid disease, but less is known about their levels in patients with Graves' ophthalmopathy (GO). It is not known whether GO is a cell-mediated or humoral autoimmune disease. We investigated whether serum cytokines are elevated in GO pati...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01335.x
更新日期：2000-09-01 00:00:00
abstract：:Lymphocytes isolated from Graves' and Hashimoto thyroid tissue by enzymatic (dispase) digestion or mechanical disaggregation were markedly different in terms of their ability to synthesize thyroid autoantibodies in culture. Dispase digestion, followed by removal of thyroid follicular cells, gave a lymphocyte populatio...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-08-01 00:00:00
abstract：:A ligand radioassay for the detection of IC which utilizes C1q, bovine conglutinin and low affinity rabbit IgM anti-human Fc in a reagent coctail, is presented. IC are first isolated from serum by precipitation in polyethylene glycol, then analysed for their ability to react with the ligand coctail. Dual-label studies...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:In the present study we examined the role of thymic stromal lymphopoietin (TSLP) in experimental autoimmune encephalomyelitis (EAE). Here, we report that TSLP knock-out (KO) mice display a delayed onset of disease and an attenuated form of EAE. This delayed onset was accompanied by a reduced number of encephalitogenic...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12621
更新日期：2015-07-01 00:00:00
abstract：:Monoclonal antibodies were produced using mice immunized with the human promyeloid cell line HL60. Two antibodies are described which identify antigens selectively expressed by myeloid cells. Studies using normal bone marrow and myeloid leukaemia cells demonstrated that one of these antibodies (AGF4.48) identifies an ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-11-01 00:00:00
abstract：:The lymphotoxin-beta receptor (LTbetaR) pathway is critical for maintenance of organized lymphoid structures and is involved in the development of colitis. To investigate the mechanisms by which LTbetaR activation contributes to the pathology of chronic inflammation we used a soluble LTbetaR-Ig fusion protein as a com...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2004.02402.x
更新日期：2004-04-01 00:00:00
abstract：:Human peripheral blood B lymphocytes, when cultured for 6 days with ovalbumin in the presence of T cells and monocytes, can differentiate into plaque-forming cells (PFC) secreting small amounts of antigen-specific IgM. Phenotype analysis using Ig-isotype and B-cell specific monoclonal antibodies revealed that the PFC ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:Two DNA-binding monoclonal antibodies (MoAb) derived from lupus mice were examined for their effects on kidney function. Antibody IV-228, reactive with single-stranded DNA (ssDNA) but not double-stranded DNA (dsDNA) significantly altered kidney function in some MRL/Mp-lpr/lpr (MRL/lpr) mice with lupus glomerulonephrit...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:Circulating immunoglobulin (Ig)G antibodies against M(2) muscarinic acetylcholine receptors (M(2) mAChR) have been implicated in Chagas' disease (ChD) pathophysiology. These antibodies bind to and activate their target receptor, displaying agonist-like activity through an unclear mechanism. This study tested the abili...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2011.04370.x
更新日期：2011-05-01 00:00:00
abstract：:The influence of human seminal plasma (SP) and whole semen (S) on the expression of natural cytotoxicity by human peripheral blood mononuclear cells (PBMNC) was examined. Marked suppression of natural cytotoxicity against K562 targets was observed when effectors were pre-treated for 1 h with SP or S diluted up to 1:40...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:Epstein-Barr virus (EBV) is a persistent virus with oncogenic capacity that has been implicated in the development of aggressive B cell lymphomas, primarily in immunosuppressed individuals, although it can be present in immunocompetent individuals. Changes in the function and clonal diversity of T lymphocytes might be...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12682
更新日期：2015-11-01 00:00:00
abstract：:Antigen- and mitogen-stimulated cytokine production by peripheral blood mononuclear cells (PBMC) of 50 pregnant women and 31 age- and sex-matched non-pregnant controls were analysed to determine whether changes in cytokine production occur during normal and pathologic human gestation. The pregnant women, consecutively...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1996.d01-809.x
更新日期：1996-10-01 00:00:00
abstract：:To define the role of quantitative graft composition and donor killer-cell immunoglobulin-like receptor (KIR) genotype in clinical outcome following unmanipulated peripheral blood stem cell transplantation (PBSCT) from human leucocyte antigen (HLA)-identical siblings, 43 consecutive transplants for haematological mali...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2007.03360.x
更新日期：2007-06-01 00:00:00
abstract：:It is unclear how breakdown in immune tolerance to the ubiquitous self-antigen pyruvate dehydrogenase complex (PDC), seen in the autoimmune liver disease PBC, gives rise to tissue damage with such a limited distribution (restricted to the liver and salivary and lachrymal glands). One property shared by these tissues i...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01403.x
更新日期：2000-12-01 00:00:00
abstract：:It has been suggested that the immune system may be responsible for the destruction of insulin secreting cells in some types of diabetes. In order to test this hypothesis, we studied the consequences of immune-mediated reactions on the function of pancreatic islet cells in vitro. A model was set up in vitro where mous...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
abstract：:An original representative of histamine-containing peptidomimetics L-glutamyl-histamine (L-Glu-Hist) was synthesized and characterized as a cytokine mimic leading to cellular responses of improved specificity. The energy-minimized 3-D conformations of L-Glu-Hist derived from its chemical structure resulted in stabiliz...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2004.02710.x
更新日期：2005-03-01 00:00:00
abstract：:The depressed cell-mediated immunity in rheumatoid arthritis was investigated in vivo by cutaneous hypersensitivity responses to seven antigens including tuberculin PPD, and in vitro by lymphocyte transformation to the latter antigen. In vivo 40% of rheumatoid patients were anergic compared to 2% of controls (P less t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：:The rabbit-passaged L strain of rinderpest virus (RV) causes the transient induction of anti-nuclear antibodies (ANA) in rabbits. It has been shown by an indirect immunofluorescence test that the target antigens of these ANA are DNA and/or DNA-histone complexes (nucleohistone). Here detailed examinations of the target...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1990.tb05483.x
更新日期：1990-12-01 00:00:00
