Abstract：
:A solid-phase platelet membrane enzyme linked immunosorbent assay (ELISA) was developed for the detection of circulating platelet binding IgG in serum. 18 out of 28 (64%) sera from patients with chronic idiopathic thrombocytopenic purpura (ITP) and 10 out of 21 (48%) sera from patients with acute ITP contained more platelet binding IgG than the mean +2 s.d. of 25 normal control sera. IgG-F(ab')2 fragments from two anti-PlA1 sera, from six out of seven positive chronic ITP sera and from four out of eight positive acute ITP sera bound to normal platelet membrane. When IgG-F(ab')2 preparations, which reacted with normal membrane, were tested against glycoprotein IIb-IIIa-deficient platelet membrane obtained from a patient with Glanzmann's thrombasthaenia, the reactions of F(ab')2 fragments from two anti-PlA1 sera, from three out of four acute ITP sera and from four out of six chronic ITP sera were reduced to control levels. It is concluded that IgG-F(ab')2 antibody binding to platelet specific antigens, some of which are associated with the glycoprotein IIb-IIIa complex, can be demonstrated not only in anti-PlA1 sera or sera from chronic ITP patients but also in sera from some patients with acute ITP. Moreover, when the IgG binding to platelet membrane of whole sera is tested, positive results may sometimes be ascribed to the presence of immune complexes.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Winiarski J,Ekelund Esubject
Has Abstractpub_date
1986-02-01 00:00:00pages
459-65issue
2eissn
0009-9104issn
1365-2249journal_volume
63pub_type
杂志文章abstract：:Type 1 diabetes mellitus (T1DM) results from death of insulin-secreting β cells mediated by self-immune cells, and the consequent inability of the body to maintain insulin levels for appropriate glucose homeostasis. Probably initiated by environmental factors, this disease takes place in genetically predisposed indivi...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/cei.12019
更新日期：2013-02-01 00:00:00
abstract：:The relation of clinical and laboratory findings at the time clinical presentation to subsequent survival was investigated in 204 patients with macroglobulinaemia. It was found that the ten following clinical and laboratory parameters were of prognostic significance and a further twelve were not. The best prognoses we...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:We have investigated the T-cell antigen receptor constant beta and alpha chain genes (TCR-C beta, -C alpha) and the immunoglobulin (Ig) heavy chain switch regions of patients with Graves' disease (GD) using restriction fragment length polymorphism (RFLP) analysis. No significant associations were found with RFLPs of e...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:The proinflammatory cytokines play a central role in mediating cellular and physiological responses, and levels may reflect immune system effectiveness. In this study, the effect of ageing on the inflammatory response was examined using a novel method to detect production of the proinflammatory cytokines, i.e. tumour ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00641.x
更新日期：1998-08-01 00:00:00
abstract：:Experimental autoimmune orchitis (EAO) was produced in C3H/He mice with as high as 100% incidence by two or three s.c. injections of 1 x 10(7) viable syngeneic testicular germ cells (TC) without resorting to adjuvants, Bordetella pertussis vaccine, or other immunological manipulations. On day 40 after the first inject...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05603.x
更新日期：1991-01-01 00:00:00
abstract：:Glucocorticoid hormones (GCH) regulate, through the apoptotic process, the negative selection of immature T cells in the thymus. Because apoptosis seems to occur also in the maintenance of peripheral tolerance, we have investigated whether GCH may induce apoptosis in human mature lymphocytes. Peripheral blood lymphocy...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1996.tb08306.x
更新日期：1996-03-01 00:00:00
abstract：:The recent report that anti-gp120 antibodies can be induced by allogeneic stimuli in experimental animals in the absence of HIV, has focused attention on the structural similarities between gp120 and MHC. Here we report that some HIV+ individuals develop antibodies which similarly react with the gp120 HIV sequence (aa...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1992.tb07923.x
更新日期：1992-11-01 00:00:00
abstract：:Coeliac disease (CD) is described as an autoimmune enteropathy associated with the presence of IgG and IgA antigliadin and antitransglutaminase autoantibodies. While of diagnostic significance, the role of these autoantibodies in the immunopathogenesis of CD is elucidated. An inappropriate T cell immune response to gl...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2003.02220.x
更新日期：2003-09-01 00:00:00
abstract：:We describe two brothers with marked leucopenia, lymphopenia, no immunologicl response to infections (no Ig production, negative PHA response and very low number of T and B lymphocytes in peripheral blood) and hypocellular marrow. They died at 12 and 8 days of life with infection (E. coli and Klebsiella, respectively)...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1979-12-01 00:00:00
abstract：:Traumatic injury and sepsis reduce HLA-DR expression on monocytes. This study assessed the effect of interferon-gamma (IFN-gamma) on the expression of HLA-DR on monocytes taken from severely injured patients. The mononuclear layer of each blood sample was incubated with either 500 units IFN-gamma or control media. The...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:Individuals with a negative intradermal reaction to tuberculin PPD have long been described in the Mycobacterium tuberculosis exposed, immune-competent population. Here, we studied PPD-specific blood T lymphocytes from these subjects for phenotypic markers relevant to skin migration, including the expression of the sk...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01128.x
更新日期：2000-01-01 00:00:00
abstract：:The clinical use of dendritic cells (DCs) to induce antigen-specific immune tolerance has been hampered by the lack of a widely acknowledged method for generating human regulatory DCs but even more so by the non-existence of reliable markers. Thus, we set out to find reliable markers that can be measured with simple m...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2009.03961.x
更新日期：2009-07-01 00:00:00
abstract：:Complement receptor type 1 (CR1) is an integral membrane protein of many haematopoietic cells and plays an important role in the clearance of complement-associated immune complexes, favouring their transport to liver and spleen macrophages. A small amount of soluble CR1 (sCR1) is also found in plasma and might origina...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00707.x
更新日期：1998-10-01 00:00:00
abstract：:In vivo anti-nuclear antibody (ANA) was observed by direct immunofluorescence microscopy in epithelial cell nuclei in forty-four biopsies from thirty-three patients. The tissue containing the ANA was macroscopically normal in twenty-seven patients. The thirty-three patients with in vivo biopsy ANA included twenty-thre...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1979-12-01 00:00:00
abstract：:In a study of 75 alveolar cell co-cultures from 55 HIV-seropositive subjects, p24 antigen production was identified by ELISA in 11 out of 26 (42%) of unseparated cell cultures, 15 out of 39 (38%) purified alveolar macrophage cultures and eight out of 10 purified alveolar lymphocyte samples. Positivity in unseparated c...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:The concept that during an immune challenge the release of glucocorticoids (GC) provides feedback inhibition on evolving immune responses has been drawn primarily from studies of autoimmune and/or inflammatory processes in animal models. The epidemic form of haemolytic uraemic syndrome (HUS) occurs secondary to infect...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2003.02057.x
更新日期：2003-02-01 00:00:00
abstract：:Allergic disorders are viewed generally as organ diseases and thus referred to organ specialists, such as the ear, nose and throat specialist for rhinitis, the pulmonologist for asthma, the dermatologist for dermatitis, and so on. Indeed, the systemic nature of allergy is made evident by the fact that the same individ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2249.2008.03712.x
更新日期：2008-09-01 00:00:00
abstract：:In vitro monoclonal immunoglobulin (mIg) production of cultured tumour cells--prepared from the bone marrow (BM) or from the peripheral blood (PB) of 40 multiple myeloma (MM) patients, 16 patients with monoclonal gammopathy of undetermined significance and two patients with M. Waldenström--was measured with an enzyme-...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:IL-1 acts on many cells as an inflammatory mediator. Its two forms, IL-1 alpha and IL-1 beta, are regulated differentially within hyperproliferative inflammatory skin conditions, such as psoriasis. While IL-1 alpha is down-regulated within psoriatic lesions, the levels of IL-1 beta are increased. However, some investi...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2001.01421.x
更新日期：2001-03-01 00:00:00
abstract：:Activin A not only stimulates the synthesis and release of pituitary follicle-stimulating hormone, but exerts various effects on haematopoietic cells, embryos, and fibroblasts. In the present study we have examined effects of activin A on IgE synthesis and cytokine production by peripheral blood mononuclear cells (PBM...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1993.tb06003.x
更新日期：1993-10-01 00:00:00
abstract：:Human IgD myeloma proteins are shown to have a high carbohydrate content (9-18%) and to exhibit the same heterogeneity in carbohydrate content and composition as myeloma protein of the other immunoglobulin classes. All IgD proteins studied contain N-acetylgalactosamine which is contained in a carbohydrate moiety attac...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1975-11-01 00:00:00
abstract：:Identification of autoimmune processes and introduction of new autoantigens involved in the pathogenesis of multiple sclerosis (MS) can be helpful in the design of new drugs to prevent unresponsiveness and side effects in patients. To find significant changes, we evaluated the autoantibody repertoires in newly diagnos...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13087
更新日期：2018-04-01 00:00:00
abstract：:IL-5 is a major factor inducing differentiation of B lymphocytes into immunoglobulin-producing cells as well as a main regulator of eosinophils. Recently, we have shown that peripheral blood mononuclear cells (PBMC) from patients with alveolar echinococcosis (AE) express IL-5 mRNA after stimulation with crude Echinoco...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1997.4031299.x
更新日期：1997-07-01 00:00:00
abstract：:Five patients with no detectable serum IgA (less than 20 mug/ml) and one patient with low serum IgA were compared to normal subjects. The number of circulating E-RFC was normal as was the lymphocyte DNA synthesis induced by PHA, Con A, and streptokinase-streptodornase. The patients had normal numbers of IgA-bearing ly...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:Clinical investigations of recombinant human acid alpha-glucosidase for the treatment of Pompe disease often reveal the appearance of therapy-specific antibodies. These antibodies could potentially interfere with recombinant human acid alpha-glucosidase efficacy and induce immunological consequences. Several immunosup...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2008.03602.x
更新日期：2008-04-01 00:00:00
abstract：:Mononuclear cells from twenty-one patients with depressed cellular reactivity were assessed for the ability to produce leucocyte inhibitory factor (LIF) and to transform after PHA stimulation, in the presence or absence of levamisole. Cells from nineteen patients failed to produce significant lymphokines when stimulat...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:The distribution and regulatory function of immunoglobulin (Ig) synthesis by monocytes were assessed using several monoclonal antibodies and a cell sorter in samples from 13 patients with multiple myeloma (MM) and nine patients with benign monoclonal gammopathy (BMG). Since all of these cells were separated directly w...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:Autoimmunity may contribute to the pathogenesis of chronic obstructive pulmonary disease (COPD). Studies have identified disease-specific autoantibodies (DSAAbs) in COPD patients, but natural autoantibodies (NAAbs) may also play a role. Previous studies have concentrated on circulating autoantibodies, but lung-associa...
journal_title：Clinical and experimental immunology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1111/cei.12565
更新日期：2015-04-01 00:00:00
abstract：:Lymphocyte subpopulations in patients with cervical carcinoma were studied before and after radiotherapy. T lymphocytes were recognized by their ability to form spontaneous rosettes with sheep erythrocytes (E rosettes). Two surface marker characteristics were used to detect B lymphocytes: receptors for activated compl...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1978-07-01 00:00:00
abstract：:Ten patients with chronic lymphatic leukaemia (CLL) were injected with the antigen phichi174 intravenously on two occasions to test their capacity for antibody production. Nine out of ten patients failed to develop a primary response, and five out of ten failed to develop a secondary response. Only one out of the ten ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1975-07-01 00:00:00
