Abstract:
:Individuals with a negative intradermal reaction to tuberculin PPD have long been described in the Mycobacterium tuberculosis exposed, immune-competent population. Here, we studied PPD-specific blood T lymphocytes from these subjects for phenotypic markers relevant to skin migration, including the expression of the skin-selective homing receptor, the cutaneous lymphocyte-associated antigen (CLA). Out of 82 patients with active tuberculosis we identified four subjects who were repeatedly PPD skin test-negative. CD4 T lymphocytes specific to mycobacterial antigens were derived from these individuals, which (i) proliferated in vitro to M. tuberculosis antigens comparably to those from PPD+ patients; (ii) secreted comparable amounts of IL-2 but lower amounts of IFN-gamma; (iii) were confined within the CLA-negative T cell subset. We conclude that the negative tuberculin reaction in a small subset of patients exposed to mycobacteria is associated with impaired production of IFN-gamma by circulating PPD-specific T cells that are lacking CLA expression. On this basis in vitro proliferation to PPD can discriminate bona fide non-responders from infected patients with a deficit in the margination of M. tuberculosis-specific T lymphocytes.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Magnani ZI,Confetti C,Besozzi G,Codecasa LR,Panina-Bordignon P,Lang R,Rossi GA,Pardi R,Burastero SEdoi
10.1046/j.1365-2249.2000.01128.xsubject
Has Abstractpub_date
2000-01-01 00:00:00pages
99-106issue
1eissn
0009-9104issn
1365-2249pii
cei1128journal_volume
119pub_type
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