当前位置： SCI文献检索 > CLINICAL AND EXPERIMENTAL IMMUNOLOGY期刊下所有文献 > Dipeptidyl peptidase IV--subcellular localization, activity and kinetics in lymphocytes from control subjects, immunodeficient patients and cord blood.

Dipeptidyl peptidase IV--subcellular localization, activity and kinetics in lymphocytes from control subjects, immunodeficient patients and cord blood.

Abstract：

:Dipeptidyl peptidase IV (DPIV), a T cell enzyme, has been implicated in the regulation of lymphocyte proliferation in response to lectins and allogeneic cells. A sensitive fluorimetric assay has been established for the enzyme and used to investigate DPIV activity, kinetics and the subcellular localization in lymphocytes from control subjects, cord blood and patients with common variable hypogammaglobulinaemia (CVH) and chronic lymphatic leukaemia (CLL). Using sucrose density gradient centrifugation and organelle marker enzyme assays, in conjunction with digitonin as a selective plasma membrane perturbant and diazotized sulphanilic acid as a non-permeant enzyme inhibitor, DPIV was shown to be a plasma membrane ecto-enzyme. A significant decrease in lymphocyte DPIV activity was observed in cord blood and in patients with CVH and CLL compared to controls. Kinetic analysis showed a marked decrease in the Vmax of lymphocyte DPIV from cord blood and patients with CVH and CLL compared to controls. The apparent Km for the substrate was unaffected in cord blood and patients with CLL. However, in patients with CVH the Km was significantly reduced. Various enzyme inhibitors showed no differences between control subjects and CVH lymphocyte activities. The decreased Km for DPIV provides further evidence for a stem cell defect rather than cell immaturity in CVH.

journal_name

Clin Exp Immunol

journal_title

Clinical and experimental immunology

authors

Harland C,Shah T,Webster AD,Peters TJ

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

201-5

issue

2

eissn

0009-9104

issn

1365-2249

journal_volume

74

pub_type

杂志文章

相关文献

CLINICAL AND EXPERIMENTAL IMMUNOLOGY文献大全
  • Cell-mediated immune response of patients with meningiomas defined in vitro by a [3H]proline microcytotoxicity test.

    abstract：:Cell-mediated cytotoxicity (CTX) of meningioma patients was assessed postoperatively by a [3H]proline microcytotoxicity test. Autologous and allogeneic tumour cells were used for prelabelling with isotope and peripheral blood lymphocytes added in a ratio of 200:1. After 60 hg the plates were washed and residual CMP co...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Pees HW,Seidel B

    更新日期：1976-05-01 00:00:00

  • Interleukin-17 and systemic lupus erythematosus: current concepts.

    abstract：:The emerging role of interleukin (IL)-17 as a hallmark proinflammatory cytokine of the adaptive immune system, produced primarily by a new T helper cell subset termed 'Th17', has received considerable attention. Differentiation of Th17 cells is driven by the simultaneous presence of transforming growth factor-beta and...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2249.2009.03944.x

    authors： Nalbandian A,Crispín JC,Tsokos GC

    更新日期：2009-08-01 00:00:00

  • Enhanced generation of reactive oxygen species in monocytes from patients with common variable immunodeficiency.

    abstract：:Monocyte and macrophage dysfunction may be important for both immunopathogenesis and clinical manifestations in subgroups of patients with primary hypogammaglobulinaemia. In the present study we examined the ability to generate reactive oxygen species (ROS) in isolated monocytes from these patients by two different me...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1994.tb06073.x

    authors： Aukrust P,Müller F,Frøland SS

    更新日期：1994-08-01 00:00:00

  • Induction of the in vitro anti-HBs response by hepatitis B surface antigen.

    abstract：:The production in vitro of antibody to hepatitis B surface antigen by peripheral blood mononuclear cells of healthy volunteers was studied after each of the three doses of hepatitis B vaccine. An in vitro hepatitis B surface antigen antibody response was successfully induced in 12% of the specimens taken over a 7 mont...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Filion LG,Saginur R

    更新日期：1988-12-01 00:00:00

  • A monoclonal antibody with specificity for Trypanosoma cruzi, central and peripheral neurones and glia.

    abstract：:Of 26 hybridomas secreting anti-Trypanosoma cruzi antibodies, two reacted with murine brain and spinal cord extracts but not other tissues. Both antibodies (5H7 and 3H3) had identical isotypes (IgM) and specificities as judged by western blotting. Antigens of molecular weight 58,000 and 37,000 in mouse brain and spina...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Snary D,Flint JE,Wood JN,Scott MT,Chapman MD,Dodd J,Jessell TM,Miles MA

    更新日期：1983-12-01 00:00:00

  • Fine specificity of anti-citrullinated peptide antibodies discloses a heterogeneous antibody population in rheumatoid arthritis.

    abstract：:Anti-citrullinated peptide antibodies (ACPA) are highly specific for rheumatoid arthritis (RA). However, the predominant B cell epitopes have not yet been defined. The aim of this study was to examine the reactivity of ACPA against different peptides derived from citrullinated proteins and to investigate whether or no...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12145

    authors： Goules JD,Goules AV,Tzioufas AG

    更新日期：2013-10-01 00:00:00

  • Self-aggregated deglycosylated IgA1 with or without IgG were associated with the development of IgA nephropathy.

    abstract：:IgA nephropathy (IgAN) is the most common primary glomerulonephritis, with various pathological phenotypes. Our previous study suggested that aberrant glycosylation of serum IgA1 was associated with different pathological phenotypes of IgAN, and substantial evidence indicated that deglycosylated IgA1 had an increased ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.03026.x

    authors： Yan Y,Xu LX,Zhang JJ,Zhang Y,Zhao MH

    更新日期：2006-04-01 00:00:00

  • Systemic inflammation in acute intermittent porphyria: a case-control study.

    abstract：:This study aimed to examine whether acute intermittent porphyria (AIP) is associated with systemic inflammation and whether the inflammation correlates with disease activity. A case-control study with 50 AIP cases and age-, sex- and place of residence-matched controls was performed. Plasma cytokines, insulin and C-pep...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12899

    authors： Storjord E,Dahl JA,Landsem A,Fure H,Ludviksen JK,Goldbeck-Wood S,Karlsen BO,Berg KS,Mollnes TE,W Nielsen E,Brekke OL

    更新日期：2017-03-01 00:00:00

  • Plasma membrane association of primary biliary cirrhosis mitochondrial marker antigen M2.

    abstract：:Antibody reactivity against the 'mitochondrial M2 antigen' was determined in sera from 10 patients with primary biliary cirrhosis (PBC), using Western blotting after SDS-PAGE separation of rat liver mitochondria (RLM) and plasma membrane proteins. The molecular weights of the major M2 antigens in rat liver mitochondri...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1991.tb05652.x

    authors： Sundin U,Sundqvist KG

    更新日期：1991-03-01 00:00:00

  • Early deficit of lymphocytes in Wiskott-Aldrich syndrome: possible role of WASP in human lymphocyte maturation.

    abstract：:Wiskott-Aldrich syndrome (WAS) is an X-linked platelet/immunodeficiency disease. The affected gene encodes WASP, a multidomain protein that regulates cytoskeletal assembly in blood cells. Patients have recurring infections, and their lymphocytes exhibit deficient proliferative responses in vitro. We report an evaluati...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2004.02409.x

    authors： Park JY,Kob M,Prodeus AP,Rosen FS,Shcherbina A,Remold-O'Donnell E

    更新日期：2004-04-01 00:00:00

  • Humoral and cell-mediated immune responses in chronic typhoid carriers.

    abstract：:Humoral and cell-mediated immune responses to Salmonella typhi were studied in 10 chronic typhoid carriers, and in healthy controls. Carriers showed impaired cellular reactivity to S. typhi antigens in the leucocyte migration inhibition test (LMI). Carriers did not show a generalized depression of cell-mediated immuni...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Dham SK,Thompson RA

    更新日期：1982-10-01 00:00:00

  • Antibodies against cytomegalovirus-induced early antigens (CMV-EA) in immunosuppressed renal-allograft recipients.

    abstract：:Antibodies against cytomegalovirus-specific early antigens (CMV-EA) were followed in sera, obtained from fifteen immunosuppressed renal-allograft recipients. Eight patients (sixty-two sera) showed seroconversion 47--137 days post-transplantation. Five patients (forty sera) with CMV antibodies at the moment of renal im...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： The TH,Andersen HK,Spencer ES,Klein G

    更新日期：1977-06-01 00:00:00

  • Enhancement of in situ immune complex formation in isolated perfused kidneys in Heymann's nephritis.

    abstract：:A preparation of the putative antibody in HN (Heymann's nephritis) lacking preformed immune complexes, obtained from the acid eluates of kidneys with HN, purified through ion exchange and gel-filtration chromatography, characterized by immunoelectrophoresis, sucrose density-gradient ultracentrifugation, 125I-Clq assay...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Makker SP,Kirson IJ,Moorthy B

    更新日期：1982-02-01 00:00:00

  • Human milk contains proteins that stimulate and suppress T lymphocyte proliferation.

    abstract：:The modulatory effect of human milk proteins from colostrum and late milk on the proliferative response of human T lymphocytes activated by mitogens (OKT3 and leucoagglutinin from Phaseolus vulgaris) and alloantigens was studied. High concentrations (10-100 micrograms/ml) of crude colostral milk proteins had an inhibi...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1990.tb08113.x

    authors： Mincheva-Nilsson L,Hammarström ML,Juto P,Hammarström S

    更新日期：1990-03-01 00:00:00

  • Dysregulation of B7.2 (CD86) expression on monocytes of HIV-infected individuals is associated with altered production of IL-2.

    abstract：:T helper (Th) responses are mediated in part by immunoregulatory cytokines and the signals delivered by the costimulatory CD28-B7 pathway. In this study, we have investigated the relationship between the regulation of B7 isoform expression on antigen-presenting cells from HIV+ individuals and the production of Th cyto...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1999.00937.x

    authors： Kumar A,Angel JB,Aucoin S,Creery WD,Daftarian MP,Cameron DW,Filion L,Diaz-Mitoma F

    更新日期：1999-07-01 00:00:00

  • The potential role for infections in the pathogenesis of autoimmune Addison's disease.

    abstract：:Autoimmune Addison's disease (AAD), or primary adrenocortical insufficiency, is a classical organ-specific autoimmune disease with 160 years of history. AAD is remarkably homogeneous with one major dominant self-antigen, the cytochrome P450 21-hydroxylase enzyme, which is targeted by both autoantibodies and autoreacti...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章,评审

    doi：10.1111/cei.13207

    authors： Hellesen A,Bratland E

    更新日期：2019-01-01 00:00:00

  • Monocyte chemoattractant protein-1 (MCP-1) inhibits the intestinal-like differentiation of monocytes.

    abstract：:Monocytes (MO) migrating into normal, non-inflamed intestinal mucosa undergo a specific differentiation resulting in a non-reactive, tolerogenic intestinal macrophage (IMAC). Recently we demonstrated the differentiation of MO into an intestinal-like macrophage (MAC) phenotype in vitro in a three-dimensional cell cultu...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.03113.x

    authors： Spoettl T,Hausmann M,Herlyn M,Gunckel M,Dirmeier A,Falk W,Herfarth H,Schoelmerich J,Rogler G

    更新日期：2006-07-01 00:00:00

  • Illegitimate RAG-mediated recombination events are involved in IKZF1 Δ3-6 deletion in BCR-ABL1 lymphoblastic leukaemia.

    abstract：:Breakpoint cluster region-Abelson murine leukaemia viral oncogene homologue 1 (BCR-ABL1), encoded by the Philadelphia (Ph) chromosome, is the characteristic of chronic myeloid leukaemia (CML) and a subset of acute lymphoblastic leukaemia (ALL). We demonstrated that expression of the Ik6 transcript, which lacked exons ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12812

    authors： Dong Y,Liu F,Wu C,Li S,Zhao X,Zhang P,Jiao J,Yu X,Ji Y,Zhang M

    更新日期：2016-09-01 00:00:00

  • Highly active anti-retroviral therapy (HAART) is associated with a lower level of CD4+ T cell apoptosis in HIV-infected patients.

    abstract：:HAART may increase CD4+ T cell counts despite a persistently detectable HIV load. The impact of HAART on apoptosis, which may play a role in the disease process in HIV-infected patients, has not been extensively studied. We performed a study to compare the level of spontaneous T cell apoptosis and anti-retroviral trea...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1999.01076.x

    authors： Roger PM,Breittmayer JP,Arlotto C,Pugliese P,Pradier C,Bernard-Pomier G,Dellamonica P,Bernard A

    更新日期：1999-12-01 00:00:00

  • Increased CD11b expression on polymorphonuclear leucocytes and cytokine profiles in patients with Kawasaki disease.

    abstract：:Clinical evidence implicates polymorphonuclear leucocytes in the pathogenesis of vasculitis in Kawasaki disease. We examined modulation of expression of adhesion molecules (CD11b and CD62L) on polymorphonuclear leucocytes and how this expression is related to serum cytokine concentrations. In 18 patients with Kawasaki...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2007.03326.x

    authors： Kobayashi T,Kimura H,Okada Y,Inoue Y,Kobayashi T,Shinohara M,Morikawa A

    更新日期：2007-04-01 00:00:00

  • Tumour necrosis factor-alpha (TNF-alpha) gene polymorphism and expression in pre-eclampsia.

    abstract：:Pre-eclampsia is an endothelial disorder and TNF-alpha has fundamental effects on endothelial cells by several means, including altering the balance between oxidant and anti-oxidant, changing the pattern of prostaglandin production and affecting expression of several cell surface components. To determine whether TNF-a...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1996.d01-647.x

    authors： Chen G,R Wilson,Wang SH,Zheng HZ,Walker JJ,McKillop JH

    更新日期：1996-04-01 00:00:00

  • Rearrangement of the human heavy chain variable region gene V3-23 in transgenic mice generates antibodies reactive with a range of antigens on the basis of VHCDR3 and residues intrinsic to the heavy chain variable region.

    abstract：:To formulate a 'logic' for how a single immunoglobulin variable region gene generates antibodies with different antigen specificity and polyreactivity, we analysed chimeric antibodies produced in transgenic mice carrying the germ-line human V3-23 gene, multiple diversity (D) and joining (J) gene segments. Hybridomas p...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2001.01380.x

    authors： Mageed RA,Harmer IJ,Wynn SL,Moyes SP,Maziak BB,Brüggemann M,MacKworth-Young CG

    更新日期：2001-01-01 00:00:00

  • Increased elastase secretion by peripheral blood monocytes in cystic fibrosis patients.

    abstract：:Morbidity and mortality in cystic fibrosis (CF) is predominantly due to destruction of pulmonary tissue. The host immune response may, in part, play a pathogenic role in pulmonary destruction in these patients. To further understand host immune response in CF, we examined the state of activation of peripheral blood mo...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1990.tb03290.x

    authors： Jones MM,Seilheimer DK,Pier GB,Rossen RD

    更新日期：1990-06-01 00:00:00

  • Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.

    abstract：:The efficacy of steroids and immunosuppressive treatments in idiopathic nephrotic syndrome (INS) hints at the implication of immune cells in the pathophysiology of the disease. Toll-like receptor (TLR) dysfunctions are involved in many kidney diseases of immune origin, but remain little described in INS. We investigat...

    journal_title：Clinical and experimental immunology

    pub_type： 临床试验,杂志文章

    doi：10.1111/cei.12659

    authors： Jamin A,Dehoux L,Dossier C,Fila M,Heming N,Monteiro RC,Deschênes G

    更新日期：2015-12-01 00:00:00

  • Diverse T cell receptor beta gene usage by infiltrating T cells in the lacrimal glands of Sjögren's syndrome.

    abstract：:Sjögren's syndrome (SS) is an autoimmune disease characterized by T cell infiltration into the salivary and lacrimal glands (LG). Previous studies on T cell receptor (TCR) usage in the minor salivary glands (SG) have yielded controversial results. We studied TCR beta gene usage of the T cells infiltrating to LG, which...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1995.tb02273.x

    authors： Mizushima N,Kohsaka H,Tsubota K,Saito I,Miyasaka N

    更新日期：1995-07-01 00:00:00

  • Comparison of the immune response against Mycobacterium tuberculosis antigens between a group of patients with active pulmonary tuberculosis and healthy household contacts.

    abstract：:The mycobacterial antigens and the factors related to protection for the development of active tuberculosis are not known. In a natural model of tuberculosis, we studied 10 patients with active pulmonary tuberculosis (non-protective immune response) and 38 healthy household contacts (protective immune response). We te...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1994.tb06233.x

    authors： Torres M,Mendez-Sampeiro P,Jimenez-Zamudio L,Teran L,Camarena A,Quezada R,Ramos E,Sada E

    更新日期：1994-04-01 00:00:00

  • The role of IL-12 in inflammatory activity of patients with rheumatoid arthritis (RA).

    abstract：:The aim of this study was to investigate the role of IL-12 in patients with RA. IL-12 (p70) and its associated cytokines were measured in sera and synovial fluid (SF) using an enzyme-linked immunosorbent method. Seven American College of Rheumatology (ACR) core set measures as well as IL-12 levels were sequentially mo...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.2000.01095.x

    authors： Kim W,Min S,Cho M,Youn J,Min J,Lee S,Park S,Cho C,Kim H

    更新日期：2000-01-01 00:00:00

  • CD4+ CD25+ transforming growth factor-beta-producing T cells are present in the lung in murine tuberculosis and may regulate the host inflammatory response.

    abstract：:CD4(+) CD25(+) regulatory T cells produce the anti-inflammatory cytokines transforming growth factor (TGF)-beta or interleukin (IL)-10. Regulatory T cells have been recognized to suppress autoimmunity and promote self-tolerance. These cells may also facilitate pathogen persistence by down-regulating the host defence r...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2007.03371.x

    authors： Mason CM,Porretta E,Zhang P,Nelson S

    更新日期：2007-06-01 00:00:00

  • The Italian quality control study for evaluation of CD4 cells in centres involved in the treatment of HIV-1 patients. Italian CD4 Quality Control Group.

    abstract：:We report on the experience of establishing a national network for a quality control programme in evaluating CD4 cell counts in most Italian centres involved in the care of patients with HIV disease. The 68 centres were divided according to their geographical location into eight groups, and twice a year (tests A and B...

    journal_title：Clinical and experimental immunology

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1046/j.1365-2249.1998.00520.x

    authors： Pandolfi F,Alario C,Girardi E,Rava L,Ippolito G,Kunkl A,Aiuti F

    更新日期：1998-03-01 00:00:00

  • Antigen-specific over-expression of human cartilage glycoprotein 39 on CD4+ CD25+ forkhead box protein 3+ regulatory T cells in the generation of glucose-6-phosphate isomerase-induced arthritis.

    abstract：:Human cartilage gp-39 (HC gp-39) is a well-known autoantigen in rheumatoid arthritis (RA). However, the exact localization, fluctuation and function of HC gp-39 in RA are unknown. Therefore, using a glucose-6-phosphate isomerase (GPI)-induced model of arthritis, we investigated these aspects of HC gp-39 in arthritis. ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12349

    authors： Tanaka Y,Matsumoto I,Inoue A,Umeda N,Takai C,Sumida T

    更新日期：2014-08-01 00:00:00