Abstract：
:The mycobacterial antigens and the factors related to protection for the development of active tuberculosis are not known. In a natural model of tuberculosis, we studied 10 patients with active pulmonary tuberculosis (non-protective immune response) and 38 healthy household contacts (protective immune response). We tested the lymphocyte proliferative response by T cell Western blotting to eight different antigen fractions and to two purified mycobacterial antigens of 30 and 64 kD. Patients with active tuberculosis recognized fractions with molecular weights of 80-114, 60-80, 28-41 and 14-19 kD. Household contacts recognized the same fractions except the 14-19 kD. The response to the 64-kD antigen was not significantly different between groups. In contrast, 10% of the patients with active tuberculosis and 73% of the household contacts responded to the 30-kD antigen. The humoral response against the 30-kD antigen by ELISA showed a significantly higher production of antibodies in tuberculosis patients compared with household contacts. We conclude that patients with active pulmonary tuberculosis develop an immune response characterized by poor proliferative response to the 30-kD antigen with a strong humoral response, whereas the opposite occurs in healthy subjects infected by Mycobacterium tuberculosis.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Torres M,Mendez-Sampeiro P,Jimenez-Zamudio L,Teran L,Camarena A,Quezada R,Ramos E,Sada Edoi
10.1111/j.1365-2249.1994.tb06233.xsubject
Has Abstractpub_date
1994-04-01 00:00:00pages
75-8issue
1eissn
0009-9104issn
1365-2249journal_volume
96pub_type
杂志文章abstract：:It has been suggested that the increase in C3 and C4 levels in jejunal perfusates of patients with Crohn's disease (CD) results from local intestinal synthesis of complement. The present study evaluated the expression of these complement genes in inflamed tissues from patients with CD. Surgically resected specimens fr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01168.x
更新日期：2000-04-01 00:00:00
abstract：:Treatment of systemic infection with Candida albicans with a combination of an antifungal agent (i.e. fluconazole) and a thymus-derived immunostimulant (i.e. thymosin alpha 1 (T alpha 1)) in mice immunosuppressed by morphine treatments was investigated. In normal mice, fluconazole given after infection with 10(6) C. a...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb06093.x
更新日期：1994-09-01 00:00:00
abstract：:A better understanding about the mechanisms involved in the pathogenesis of type 2 diabetes mellitus (T2D) showed that inflammatory cytokines such as tumour necrosis factor (TNF) and interleukin (IL)-1β play a pivotal role, mirroring data largely reported in rheumatoid arthritis (RA). IL-1β is produced mainly by monoc...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12667
更新日期：2015-10-01 00:00:00
abstract：:Peripheral blood natural killer (NK) activity was analysed from 32 asthma patients and 13 control donors. Male patients and female patients with atopic asthma had significantly stronger NK activity against the leukaemic cell line K-562. Fractionation of patient mononuclear cells by discontinuous density gradient centr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:Recently we described the occurrence of B cells producing polyspecific natural IgM with anti-tumour specificity in the spleen of non-tumour-bearing individuals as well as in fetal organisms. Immunoprecipitation and 2-D electrophoresis showed the binding of such antibodies to a 55-kD (pI 6.0) membrane surface glycoprot...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb05561.x
更新日期：1995-03-01 00:00:00
abstract：:Activin A is a multi-functional cytokine with a potent stimulation on erythroid cell differentiation in the bone marrow. The actions of activin A are determined by a balance of the levels of activin A and its inhibitor, follistatin (FS). However, the regulation of its actions in the bone marrow has been unclear. Here ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2001.01644.x
更新日期：2001-10-01 00:00:00
abstract：:A method is described to assess antigenic cross-reactivity between soluble immune complexes precipitated from sera with polyethylene glycol. The precipitated complex from one serum was dissociated in acid and used to coat a plastic cup. Radioiodinated complexes from another serum were dissociated in the cup, neutraliz...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1979-09-01 00:00:00
abstract：:Commercial cellular tests are used to diagnose Lyme borreliosis (LB), but studies on their clinical validation are lacking. This study evaluated the utility of an in-house and a commercial enzyme-linked immunosorbent spot (ELISpot) assay for the diagnosis of Lyme neuroborreliosis (LNB). Prospectively, peripheral blood...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13393
更新日期：2020-03-01 00:00:00
abstract：:Streptococcus suis serotype 2 is known to be a major pathogen of swine, causing mainly meningitis. It is also a zoonotic agent leading predominantly to meningitis in humans working in close contact with pigs. In this study, we investigated the ability of S. suis to up-regulate the expression of adhesion molecules invo...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2003.02189.x
更新日期：2003-07-01 00:00:00
abstract：:Stress known to stimulate sympathetic activity, as well as the hypothalamic-pituitary-adrenal axis (HPA), produces a significant increase in adrenomedullin (AdM) levels, suggesting a regulatory or protective role for AdM in countering HPA activation that follows a variety of stressors. Stressors can modulate the secre...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2010.04156.x
更新日期：2010-07-01 00:00:00
abstract：:The capacity of B cells from patients with chronic lymphocytic leukaemia (CLL) to produce and secrete immunoglobulin following mitogen stimulation was investigated using sensitive radioimmunoassays for mu, gamma, alpha, kappa, and lambda immunoglobulin chains. Lymphocytes from seven of the 11 patients studied secreted...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-03-01 00:00:00
abstract：:Lung cancer is strongly associated with cigarette smoking. More than 20 lung carcinogens have been identified in cigarette smoke and one of the most abundant is 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). We hypothesized that NNK modulates alveolar macrophage (AM) mediator production, thus contributing to ca...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2003.02142.x
更新日期：2003-05-01 00:00:00
abstract：:The membrane attack complex (MAC) of complement is activated by immune and non-immune mechanisms in the kidney. MAC has been found associated with glomerular immune deposits, but also to cell remnants, particularly along tubules and in vessel walls. Clusterin and S-protein (vitronectin) bind to MAC, rendering it cytol...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1992.tb06459.x
更新日期：1992-06-01 00:00:00
abstract：:Previous studies using in vitro systems with various stimuli have shown that PBMC from patients with AD show increased levels of IL-4 but decreased levels of interferon-gamma (IFN-gamma) compared with PBMC from normal controls. However, in vitro conditions do not always mimic the in vivo condition. We therefore believ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1997.d01-1015.x
更新日期：1997-05-01 00:00:00
abstract：:In order to gain information about the nature of the defect in X-linked severe combined immunodeficiency (XSCID), we investigated gene expression in different lymphoid and haematopoietic cells of female carriers by looking for non-random X chromosome usage. We have shown non-random X chromosome usage in T lymphocyte e...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05579.x
更新日期：1991-01-01 00:00:00
abstract：:IgA nephropathy (IgAN) is the most common primary glomerulonephritis, with various pathological phenotypes. Our previous study suggested that aberrant glycosylation of serum IgA1 was associated with different pathological phenotypes of IgAN, and substantial evidence indicated that deglycosylated IgA1 had an increased ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2006.03026.x
更新日期：2006-04-01 00:00:00
abstract：:We have recently demonstrated that peripheral blood monocytes from patients with multiple sclerosis (MS) have a defect in stimulating autologous and allogeneic T lymphocytes. This defect was found to correlate with disease activity. In this report we demonstrate that prothymosin alpha (ProT alpha), a rat thymus fracti...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：:The distribution and regulatory function of immunoglobulin (Ig) synthesis by monocytes were assessed using several monoclonal antibodies and a cell sorter in samples from 13 patients with multiple myeloma (MM) and nine patients with benign monoclonal gammopathy (BMG). Since all of these cells were separated directly w...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:To identify and measure the spontaneous cell-mediated cytotoxicity of natural killer cells, we used the lysis of an established lymphoblastoid cell line as target in a 4-hr 51Cr-release assay. Mononuclear cell suspensions of the peripheral blood of thirty-four patients with Crohn's disease (group CD), eleven patients ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:The effect of cyclosporin A (CyA) immunosuppression on the murine thymic microenvironment and T lymphocyte development has been analysed using monoclonal antibodies to epithelial and lymphocyte subpopulations, macrophages and major histocompatibility complex (MHC) class II antigens in immunohistochemistry and flow cyt...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：:The structural characteristics of autologous red blood cell (ARBC) receptors on human peripheral blood lymphocytes (PBL) induced by phytohaemagglutinin (PHA) were examined. Tunicamycin, which is known to be a blocker of the protein glycosylation of N-glycosidic type glycoprotein, significantly inhibited the production...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：:In essential mixed, type II, cryoglobulinaemia (EMC) monoclonal autoantibodies with rheumatoid factor activity are synthesized at an accelerated rate by non-malignant B lymphocytes. In order to determine if the high cryoglobulin production rate is related to a clonal B cell expansion, cell surface markers of periphera...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:The Km for AMP for 5' nucleotidase was increased in lymphocytes from patients with common variable (CVH) and sex linked (XLH) hypogammaglobulinaemia and from patients with chronic lymphatic leukaemia (CLL): the Vmax of the latter was low. The kinetic constants for cord blood lymphocytes are similar to adults and the K...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：:Features of tissue damage in invasive amoebiasis, in particular polymorphonuclear neutrophil (PMN) degranulation and vasculitis, bear resemblance to that seen in Wegener's granulomatosis, the latter being associated with the presence of anti-neutrophil cytoplasmic antibodies (ANCA). We therefore tested sera from patie...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb06578.x
更新日期：1994-07-01 00:00:00
abstract：:The clinical use of dendritic cells (DCs) to induce antigen-specific immune tolerance has been hampered by the lack of a widely acknowledged method for generating human regulatory DCs but even more so by the non-existence of reliable markers. Thus, we set out to find reliable markers that can be measured with simple m...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2009.03961.x
更新日期：2009-07-01 00:00:00
abstract：:The respective roles of thymus-derived and bone marrow-derived lymphocytes in the immune response to DNA was investigated in mice reconstituted with various cell populations using cell transfers. A helper effect of thymus-derived cells in the immune response to DNA was suggested by experiments performed in athymic nud...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：:An experimental model of immunological deficiency obtained by treating mice for 6 months with serum of human blood drawn from different healthy individuals has been studied. The results show that an alteration of a circulating lymphocyte population with alterations of the ratio CD4+/CD8+ appeared in mice stimulated fo...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1997.tb08315.x
更新日期：1997-11-01 00:00:00
abstract：:The spontaneous lysis of target cells sensitive to natural killer (NK) activity is accomplished in two distinct phases: (i) binding between target and effector cells and (ii) post-binding events leading to target cell destruction. To test the hypothesis that cell surface carbohydrate(s) might be involved in recognitiv...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
abstract：:Antibody-dependent cellular cytotoxicity (ADCC) was investigated as s possible mechanism of pathogenesis in recurrent aphthous ulceration (RAU). ADCC induced by mononuclear cells from patients at various stages of RAU was compared with ADCC induced by mononuclear cells from paired controls. Chicken red blood cells (Ch...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-06-01 00:00:00
abstract：:Sensorineural hearing loss is a common problem in the otolaryngologist's practice, with autoimmune disease of the inner ear being one possible cause. The restoration of auditory function in some patients following immunosuppressive therapy has created a desire to define and understand this disease better. Because of t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb06591.x
更新日期：1994-07-01 00:00:00
