Abstract：
:A technique is described which shows how to obtain a suspension of only progressively motile spermatozoa, from semen, by active penetration into an overlying buffer layer. About 60% of the number of motile spermatozoa in the original semen sample can be obtained as an output in the removed buffer layer. With this method many more semen samples, in particular those with less than 70% motile spermatozoa, can be used than otherwise would have been possible. Moreover, with such a suspension of only motile spermatozoa the sensitivity of the micro-immobilization test is substantially improved, due to the absence of non-specific clumping, the minimization of specific tail-to-tail agglutination and an optimal end-point reading because of the absence of background immotility. As the complement source, a 1:8 dilution of rabbit or guinea-pig serum in human AB serum was found to be most suitable. The 1 microliter sperm suspension is initially mixed with 2 microliter of serum and incubated for 30 min, and then a further 2 microliter volume of complement is added, which is incubated for 2 hr with the serum-sperm mixture, and the test is read under an inverted microscope.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Hellema HW,Rümke Psubject
Has Abstractpub_date
1978-01-01 00:00:00pages
1-11issue
1eissn
0009-9104issn
1365-2249journal_volume
31pub_type
杂志文章abstract：:Anti-neutrophil cytoplasmic antibodies (ANCA) against proteinase 3 (PR3) are strongly associated with Wegener's granulomatosis (WG) and are thought to be involved in its pathogenesis. Levels of PR3-ANCA do not always correspond to clinical disease activity. To investigate the relationship between functional effects of...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2002.01926.x
更新日期：2002-09-01 00:00:00
abstract：:The number of NK T cells was measured in relation to the Th1/Th2 imbalance observed in RA. Peripheral blood samples of patients with RA (n = 60) and healthy controls (n = 36) were stained with anti-NK receptor 1A (anti-NKR-P1A), anti-CD56, and anti-CD3 MoAbs, and examined by three-colour flow cytometry. NK T (NKR-P1A+...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.01018.x
更新日期：1999-10-01 00:00:00
abstract：:The effects of 2.5-40 mg/kg nandrolone decanoate (ND) on the development and growth of mononuclear cell infiltrations in the submandibular glands of NZB/W (B/W) mice have been studied using a quantitative histological method. Injections with ND for 9 months, once every 3 weeks, starting at 4 weeks of age, reduced the ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
abstract：:Recognition of the protein A-like activity of some strains of group A streptococci has thrown doubt on much previous work suggesting antigenic cross-reactions between these streptococci and mammalian tissues. The strains used in our previous studies have now been examined by the mixed reverse passive antiglobulin reac...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-10-01 00:00:00
abstract：:Maternal transmission of islet autoantibodies to children born to mothers with type 1 diabetes (T1D) has been shown to protect from autoantibodies and diabetes development later in life. However, the factors conferring disease protection are poorly understood. The aim of this study was to evaluate comparatively proinf...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,多中心研究
doi：10.1111/j.1365-2249.2006.03243.x
更新日期：2006-12-01 00:00:00
abstract：:The pattern of T cell receptor V gene expression by lymphocytes from rheumatoid synovial fluid and paired peripheral blood samples was compared using a polymerase chain reaction (PCR)-based assay. Eight rheumatoid arthritis (RA) patients who had varying durations of disease (from 2 to 20 years) were studied. In all pa...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1992.tb05865.x
更新日期：1992-12-01 00:00:00
abstract：:Young rats were not able to expel adult N. brasiliensis infections even when the worms were damaged by antibodies and the young rats were given all the cellular components (sensitized lymphocytes and bone marrow cells) shown to be necessary for the expulsion of antibody-damaged worms from adult rats. In contrast, most...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1975-07-01 00:00:00
abstract：:Cytotoxic T lymphocytes (CTL) and natural killer (NK) cells have a key role in host defence against infectious pathogens, but their response to bacteria is not well characterized. Non-typeable Haemophilus influenzae is a major cause of respiratory tract infection including otitis media, sinusitis, tonsillitis and chro...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2008.03667.x
更新日期：2008-06-01 00:00:00
abstract：:Breakpoint cluster region-Abelson murine leukaemia viral oncogene homologue 1 (BCR-ABL1), encoded by the Philadelphia (Ph) chromosome, is the characteristic of chronic myeloid leukaemia (CML) and a subset of acute lymphoblastic leukaemia (ALL). We demonstrated that expression of the Ik6 transcript, which lacked exons ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12812
更新日期：2016-09-01 00:00:00
abstract：:Nucleotide-binding, oligomerization domain (NOD)-like receptor family, pyrin domain containing 3 (NLRP3) gene polymorphism was reported to be associated with susceptibility, disease activity or anti-tumour necrosis factor (TNF) treatment response in rheumatoid arthritis (RA). However, the roles of NLRP3 inflammasome i...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13167
更新日期：2018-11-01 00:00:00
abstract：:The human immune response to the 10-kD M. tuberculosis protein was studied by a competition ELISA using monoclonal antibody (MoAb) SA-12. Twenty-five per cent of the sera from 20 patients with tuberculosis and none from 21 control subjects inhibited binding of SA-12 to the 10-kD antigen. To characterize the antigenic ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05765.x
更新日期：1991-10-01 00:00:00
abstract：:Although both envelope glycoproteins of the hepatitis C virus, E1 and E2/NS1, show a high degree of sequence variation, the E1 protein includes a well conserved domain, which may be functionally important. We have analysed the human B cell response to a peptide fragment from amino acid residues 314-330 (EP3) covering ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb08351.x
更新日期：1995-08-01 00:00:00
abstract：:Burkholderia pseudomallei is the causative agent of melioidosis. Historically believed to be a relatively rare human disease in tropical countries, a recent study estimated that, worldwide, there are approximately 165 000 human melioidosis cases per year, more than half of whom die. The bacterium is inherently resista...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/cei.13286
更新日期：2019-05-01 00:00:00
abstract：:A monoclonal IgM smooth muscle antibody secreted by a hybrid (MMI-1) of mouse plasmacytoma NS-1 with spleen cells from mouse immunized with Treponema pallidum was detected by indirect immunofluorescence tests on frozen tissue sections and on acetone fixed monolayers of rat and human fibroblasts. The antibody did not r...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-06-01 00:00:00
abstract：:Lymphocytes from human fetuses and newborns strongly, regularly, and non-specifically suppress the proliferation of PHA stimulated peripheral blood mononuclear leucocytes. The suppression is prostaglandin (PG)-dependent. Our present investigation clearly indicates that the suppression is associated with neonatal T ver...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:EAU is a model of ocular inflammatory disease. EAU resembles another T cell-mediated autoimmune disease--experimental allergic encephalomyelitis--since both have increased expression of MHC class II molecules in the target tissue, can be adoptively transferred by activated CD4+ T cells and are inhibited by cyclosporin...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2249.1992.tb06926.x
更新日期：1992-08-01 00:00:00
abstract：:Dendritic cells (DCs) are powerful antigen-presenting cells capable of maintaining peripheral tolerance. The possibility to generate tolerogenic DCs opens new therapeutic approaches in the prevention or remission of autoimmunity. There is currently no treatment inducing long-term tolerance and remission in type 1 diab...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2009.04082.x
更新日期：2010-05-01 00:00:00
abstract：:Recent experimental data indicate a pathogenic role of complement activation in congestive heart failure (CHF). The aim of this study was to evaluate contact and complement systems activation in patients hospitalized for an acute episode of CHF. Forty-two of 80 consecutive patients admitted at our hospital with confir...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13011
更新日期：2017-11-01 00:00:00
abstract：:Glomerulonephritis with organized microtubular monoclonal immunoglobulin deposits (GOMMID) and glomerulonephritis related to type I cryoglobulin are well-known but rare complications of B cell derived chronic lymphocytic leukaemia. In these disorders, monoclonal Ig have never been studied at the molecular level. We co...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2002.01896.x
更新日期：2002-07-01 00:00:00
abstract：:Hypersensitivity to Chironomidae (non-biting midges) has been a problem in Northern Sudan since about 1927 and is probably due to the working of dams which have produced lake-like conditions on parts of the Blue and main Niles where breeding has evidently increased. Studies were undertaken to determine whether this hy...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1978-10-01 00:00:00
abstract：:Antigens present in the products released by the larval stage of schistosome (SRP-A) were shown to induce a strong cytotoxic and protective IgE response both in the rat and the monkey. T cell lines and clones specific for SRP-A or 26 kD antigens which are the main target of the cytotoxic IgE have been derived. The pas...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
abstract：:Fifty sera which gave ring-like nuclear staining in immunofluorescence on rat liver tissue sections were characterized. Using immunoprecipitation and Western blotting we showed that 10 of the sera contained antibodies to 200 kD polypeptide(s) of nuclear envelope. Clinical and biological data were available for nine of...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:Anaemia of chronic disease (ACD) is frequently found in rheumatoid arthritis (RA). In the pathogenesis of ACD both cytokines, such as tumour necrosis factor-alpha (TNF-alpha), IL-1 and IL-6 as well as a relative deficiency of erythropoietin (EPO), are thought to play a key role. In the present study the role of IL-6 i...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1996.d01-622.x
更新日期：1996-02-01 00:00:00
abstract：:A rabbit antiserum has been prepared using peripheral mononuclear cells from a patient with common variable hypogammaglobulinaemia (CVH) who had demonstrable suppressor cells in vitro. This antiserum (anti-Ck), after absorption with pooled normal glass-non-adherent cells and platelets, showed selective reactivity with...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-12-01 00:00:00
abstract：:Uveitis (intraocular inflammation) is a leading cause of loss of vision. Although its aetiology is largely speculative, it is thought to arise from complex genetic-environmental interactions that break immune tolerance to generate eye-specific autoreactive T cells. Experimental autoimmune uveitis (EAU), induced by imm...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13021
更新日期：2017-12-01 00:00:00
abstract：:Goodpasture (GP) antigens, protein components reactive with human autoantibodies against glomerular basement membrane (GBM), were identified in human alveolar basement membrane (ABM) using an enzyme-linked immunoassay (ELISA), Western blotting and immunoprecipitation. All six anti-GBM antisera studied, three obtained ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:Recurrent respiratory papillomatosis (RRP) is characterized by benign exophytic lesions of the respiratory tract caused by the human papillomavirus (HPV), in particular low-risk HPV6 and HPV11. Aggressiveness varies greatly among patients. Surgical excision is the current standard of care for RRP, with adjuvant therap...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/cei.13387
更新日期：2020-02-01 00:00:00
abstract：:Although pituitary hormones are known to affect immune function, treated hypopituitarism is not a recognized cause of immune deficiency in humans. We set out to assess integrity of baseline and stimulated immune function in severely hypopituitary adults. Twenty-one panhypopituitary adults (group 1), on stable pituitar...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2009.03881.x
更新日期：2009-05-01 00:00:00
abstract：:The lymphocyte activating properties of a membrane proteoglycan (MPG) extracted from a mutant non-encapsulated strain of Klebsiella pneumoniae (Kp) (biotype a I-145) were investigated. Kp MPG induced a strong proliferative response of BALB/c spleen cells and Peyer's patches cells. Thymidine incorporation was dose-rela...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:Immune complexes (IC) in sera and synovial fluid (SF) from patients with juvenile (rheumatoid) arthritis (JA) were isolated by making use of polyethylene glycol (PEG) to precipitate IC and then using staphylococcal protein A to separate the IC. The isolated IC were compared to IC in sera analysed by sucrose density gr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
