Abstract:
:Immune complexes (IC) in sera and synovial fluid (SF) from patients with juvenile (rheumatoid) arthritis (JA) were isolated by making use of polyethylene glycol (PEG) to precipitate IC and then using staphylococcal protein A to separate the IC. The isolated IC were compared to IC in sera analysed by sucrose density gradients and measured by the C1q solid phase assay (ClqSPA). Isolated IC from sera of 10 of 16 JA patients demonstrated significant 19S IgM rheumatoid factor (RF) titres in their acid elutes utilizing the haemolytic assay. IgM and IgG were detected by low level radial immunodiffusion in the acid eluate of sera in all 10 patients who had significant RF titres. In isolated IC from SF, three of five JA patients had significant 19S IgM RF titres detected in their acid eluates. By sucrose density gradient analysis of these sera, elevated levels of IC detected by ClqSPA were found in the fractions of greater than or equal to 19S comparable to the previous isolated 19S IgM-IgG complexes. This detection of IC like material containing 19S IgM and IgG by the present method further supports the participation of classic and hidden 19S IgM RF in IC formation in JA patients.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Moore TL,Dorner RW,Sheridan PW,Zuckner Jsubject
Has Abstractpub_date
1984-05-01 00:00:00pages
247-52issue
2eissn
0009-9104issn
1365-2249journal_volume
56pub_type
杂志文章abstract::Acute RSV infection in infancy may produce some asthma-like symptoms and may be followed by a recurrent wheeze later in childhood. It has been proposed that RSV infection stimulates type-2 cytokine responses, resembling those found in atopy and asthma. Peripheral blood cells were obtained from RSV-infected infants (n ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
doi:
更新日期:1996-05-01 00:00:00
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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更新日期:2003-07-01 00:00:00
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journal_title:Clinical and experimental immunology
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更新日期:2004-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-08-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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更新日期:1999-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1980-10-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
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journal_title:Clinical and experimental immunology
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journal_title:Clinical and experimental immunology
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更新日期:1998-11-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
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