Abstract:
:Porphyromonas gingivalis cysteine proteinases (gingipains) have been associated with virulence in destructive periodontitis, a disease process that has been linked with Th2 pathways. Critical in maintaining Th2 activity is the response of B lymphocytes to environmental interleukin (IL)-4, a cytokine that also counteracts Th1-cell differentiation. Here we demonstrate that while the gingipains effectively degrade interleukin (IL)-4 under serum-free conditions, limited hydrolysis was observed in the presence of serum even after prolonged incubation. Gingipains up-regulated CD69 expression directly in purified peripheral blood B cell preparations. Further, the induction of IL-4 receptor expression on B cells by gingipains correlates with B cell activation, which is also manifested by a mitogenic response. These results suggest that the gingipains of P. gingivalis act during the early stage of B-cell growth as a competence signal, whereby sensitized B cells might become more responsive to further challenge in the disease-susceptible individual.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Yun LW,Decarlo AA,Collyer C,Hunter Ndoi
10.1046/j.1365-2249.2003.02287.xsubject
Has Abstractpub_date
2003-11-01 00:00:00pages
295-302issue
2eissn
0009-9104issn
1365-2249pii
2287journal_volume
134pub_type
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