Epitope mapping of an HLA-B27 monoclonal antibody that also reacts with a 35-kD bacterial outer-membrane protein.

Abstract:

:Ye-3 is an HLA-B27-specific murine monoclonal antibody recognizing the heat-modifiable protein of Enterobacteriaceae. Here we used recombinant hybrid molecules between the HLA-B7 and HLA-B27 antigens to delineate the epitope recognized by Ye-3. Results of these experiments indicated that the segment of HLA-B*2705 spanning residues 77-81 was critical to the reactive epitope. It is known to be a major serological and functional recognition site of HLA-B*2705 and our data give support for its involvement also in the serological cross-reactivity with bacterial antigens.

journal_name

Clin Exp Immunol

authors

Toubert A,Hamachi M,Raffoux C,Park MS,Yu DT

doi

10.1111/j.1365-2249.1990.tb05397.x

subject

Has Abstract

pub_date

1990-10-01 00:00:00

pages

16-20

issue

1

eissn

0009-9104

issn

1365-2249

journal_volume

82

pub_type

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