Abstract:
:Peripheral leucocytes and platelets from twenty healthy volunteers were incubated in vitro with radiographic contrast media (diatrizoate-Hypaque, iothalamate-Conray and iodipamide-Cholografin) under varying conditions. All radiographic contrast media (RCM) were able to induce histamine release from peripheral leucocytes and the release reaction was dose-dependent. There were individual differences in the sensitivity of leucocytes to different RCM. The highest values (up to 80% histamine release) were found with high concentrations (0.07-0.3 M) of diatrizoate. The addition of normal human serum (NHS) to the reaction mixture led to a further increase in histamine release (P is less than 0.01), probably due to complement activation. The mechanism seems to be mediated by proteins of the alternative pathway, because serum depleted of complement components (factor B, factor D, properdin) did not show this synergistic effect. IgG-depleted serum, however, was able to show the augmented release reaction. Washed platelets incubated with RCM released serotonin in a dose- and time-dependent reaction. The most powerful serotonin-releasing RCM was found to be iodipamide, which produced a release reaction in all people investigated at concentrations of 0.04-0.09 M, while diatrizoate and iothalamate were effective only in half of the tested individuals at high concentrations (0.3 and 0.2 M respectively). The addition of plasma proteins to the reaction mixture inhibited the serotonin release quantitatively. There was no difference in inhibitory potency between autologous and heterologous plasma or serum; sera depleted of various complement components showed similar effects as NHS. The serotonin release was not due to platelet lysis, as determined by the concentration of lactic dehydrogenase present in the supernatant during serotonin release. Incubation of the leucocytes with RCM produced ultrastructural changes, including degranulation of basophils, aggregation of platelets and infiltration of the aggregates by polymorphonuclear leucocytes. The most prominent changes were observed when complement was present in the reaction mixture.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Ring J,Arroyave CM,Frizler MJ,Tan EMsubject
Has Abstractpub_date
1978-04-01 00:00:00pages
105-18issue
1eissn
0009-9104issn
1365-2249journal_volume
32pub_type
杂志文章abstract::To gain insight into possible determinants of in vivo polyclonal B cell activation seen in rheumatoid arthritis (RA), we enumerated immunoglobulin secreting cells appearing in cultures of peripheral blood mononuclear cells that were stimulated with pokeweed mitogen (PWM) or a newly described polyclonal B cell activato...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::Autoantibodies to nuclear structures are a hallmark of systemic lupus erythematosus (SLE), including autoantibodies to nuclear protein high mobility group box 1 (HMGB1). HMGB1 consists of three separate domains: box A, box B and an acidic tail. Recombinant box A acts as a competitive antagonist for HMGB1 and might be ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12951
更新日期:2017-06-01 00:00:00
abstract::It has recently been proposed that theophylline activates T suppressor systems in vivo and this evidence is further supported by the ability of the drug to attenuate the allograft rejection in humans and in experimental animals. In this study the acute effects of aminophylline on human peripheral blood lymphocyte (PBL...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1984-09-01 00:00:00
abstract::Mouse-rosette formation was investigated in a variety of leukaemias and lymphomas, and it was found that this phenomenon was restricted to the B lymphocytes of chronic lymphocytic leukaemia and hairy-cell leukaemia. Eighteen of nineteen cases of chronic lymphocytic leukaemia expressed the mouse receptor, but only eigh...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
abstract::Autoantibodies directed against cytoplasmic antigens of neutrophils (ANCA), especially those with specificity for proteinase 3 (PR-3) and myeloperoxidase, are valuable markers for differential diagnosis and monitoring of disease activity in Wegener's granulomatosis (WG) and other vasculitides. Till now, several concep...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1994.tb06110.x
更新日期:1994-09-01 00:00:00
abstract::The effect of IL-4 and transforming growth factor-beta (TGF-beta) on immunoglobulin secretion in vitro by peripheral blood mononuclear cells (PBMC) or purified B cells activated with murine EL4 thymoma cells and phorbol myristate acetate (PMA) was investigated. As previously reported, IL-4 induced IgE and IgG4 secreti...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1992.tb06942.x
更新日期:1992-08-01 00:00:00
abstract::Thirty-three patients with chronic hepatitis C/non-A, non-B were included in a randomized controlled study of interferon-alpha 2b (IFN-alpha 2b) treatment, 3 x 10(6) U three times weekly for 36 weeks. Using an immunoperoxidase technique, frozen liver biopsy specimens were examined with MoAbs for the presence of T help...
journal_title:Clinical and experimental immunology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1365-2249.1992.tb02999.x
更新日期:1992-03-01 00:00:00
abstract::Vacuum-assisted closure involving the implantation of polyvinyl alcohol foam is a technique recently developed for the treatment of patients suffering from either wound infection or chronic wounds. This method has been shown to improve and accelerate wound healing. However, little is known about the cell populations t...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2001.01547.x
更新日期:2001-06-01 00:00:00
abstract::Schizophrenic patients are reported to have immunological dysfunction, however, the immune response to surgery in schizophrenic patients remains unclear. We measured plasma interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor-alpha (TNF-alpha) before, during and after colectomy, hemicolectomy and sigm...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2001.01581.x
更新日期:2001-07-01 00:00:00
abstract::A phenotypic and functional analysis of the circulating natural killer (NK) cell population was carried out in a series of patients with hairy cell leukaemia (HCL). The overall mean NK activity of both the mononuclear and T cell fractions was reduced compared to that of normal controls (466 lytic units (lu) v 573 lu a...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::Splenectomized patients are highly susceptible to infections with capsulated bacteria and an impaired response to vaccination with bacterial polysaccharides has frequently been observed in these individuals. Based partly on experimental animal data, an important role for the spleen in the production of specific anti-c...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1986-11-01 00:00:00
abstract::Depletion of monocytes from peripheral blood mononuclear cells of patients with systemic lupus erythematosus (SLE) resulted in a decreased anti-DNA antibody synthesis in vitro. The addition of monocytes restored the response by lymphocytes and the maximum response in the presence of 2.5-8% of monocytes, whereas greate...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract::In vitro lymphocyte function of 13 patients with selective IgA deficiency was studied. IgG, IgA and IgM secretion by pokeweed mitogen-stimulated peripheral blood lymphocytes from normal donors and IgA deficient patients was measured by an enzyme-linked immunosorbent assay. Addition of concanavalin A or hydrocortisone ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-08-01 00:00:00
abstract::Graves' disease (GD) is characterized by the presence of autoantibodies against the TSH-receptor (TSH-R) which are pathogenic and, upon binding to the receptor, trigger intracellular signal transduction. The autoantibodies are oligoclonal and as they are responsible for disease activity, their characterization would l...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1999.01042.x
更新日期:1999-11-01 00:00:00
abstract::The aim of this study was to analyse and characterize immunoglobulins in CIC and serum from patients with anti-cardiolipin antibodies. CIC from five patients were isolated by gradient centrifugation and gel filtration. The distribution between serum and CIC of immunoglobulin reactivity against different phospholipids ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1997.d01-973.x
更新日期:1997-04-01 00:00:00
abstract::Cryostat sections of dermal lesions from 30 untreated leprosy patients were studied by indirect immunofluorescence using monoclonal antibodies defining T cell subsets and Ia like antigens. Most lymphocytes in leprosy lesions were positive for OKT3 and Ia like antigens indicating thereby the presence of activated T cel...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1983-03-01 00:00:00
abstract::Sjögren's syndrome (SS) is characterized by a focal periductal salivary gland infiltrate consisting mainly of T and B lymphocytes. Most of the T cells bear the memory CD4+ Th1-like phenotype and express high levels of class II, though CD8+ cells are also present. We have studied 17 labial salivary gland and 15 periphe...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1996.d01-623.x
更新日期:1996-02-01 00:00:00
abstract::A technique is described which shows how to obtain a suspension of only progressively motile spermatozoa, from semen, by active penetration into an overlying buffer layer. About 60% of the number of motile spermatozoa in the original semen sample can be obtained as an output in the removed buffer layer. With this meth...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::We report a study on immunoregulation of in vitro antibody to hepatitis B surface antigen (anti-HBs) synthesis induced by pokeweed mitogen (PWM) from peripheral blood mononuclear cells (PBMC) in chronic hepatitis B surface antigen (HBsAg) carriers and in 'high responders', (anti-HBs RIA ratio greater than or equal to ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::The proliferative responses to stimulation with phytohaemagglutinin (PHA) in cultures of lymphocytes from newly calved and non-pregnant cattle were compared. Lymphocyte cultures from newly calved cows were seen to respond less to PHA than cultures from the non-pregnant cows. It is suggested that this depression in res...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1977-07-01 00:00:00
abstract::Complement induces mixed aggregation of murine spleen cells bearing C3 receptors. In the present studies, we show with immunofluorescent techniques that these aggregates are formed by T and B lymphocytes and myeloid cells, B cells constituting the predominant component in normal mice. There are striking changes in the...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00
abstract::To elucidate the in vivo mechanisms involved in the impairment in pulmonary defence as the result of treatment with glucocorticoids, we established fatal pneumonia with bacteraemia in dexamethasone (DEX)-treated mice by means of an intratracheal challenge of Pseudomonas aeruginosa. An increased neutrophil influx was o...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2001.01656.x
更新日期:2001-11-01 00:00:00
abstract::Intra-articular injection of highly purified or recombinant interleukin 1 (IL-1) into the rabbit knee induces a transient synovitis with leucocytic infiltration into the synovial lining and joint cavity and loss of proteoglycan from articular cartilage. Tumour necrosis factor alpha (TNF-alpha), which has many of the a...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::Measles CF antibodies have been examined in the sera of patients with a variety of clinical disorders associated with the production of autoantibodies. Previous reports of high-titre reactions in DLE and chronic active hepatitis have been confirmed, the titres in the latter disorder being particularly elevated. Mean a...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1976-06-01 00:00:00
abstract::Identification of autoimmune processes and introduction of new autoantigens involved in the pathogenesis of multiple sclerosis (MS) can be helpful in the design of new drugs to prevent unresponsiveness and side effects in patients. To find significant changes, we evaluated the autoantibody repertoires in newly diagnos...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.13087
更新日期:2018-04-01 00:00:00
abstract::Previous studies show that endoplasmic reticulum-associated aminopeptidase (ERAP1/ERAP2) and runt-related transcription factor 3 (RUNX3) gene polymorphisms are associated with AS (ankylosing spondylitis) in European Caucasians. However, contradictory results were reported in different Asian populations. The purpose of...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.13121
更新日期:2018-07-01 00:00:00
abstract::Dysfunction of cytotoxic activity of T and natural killer (NK) lymphocytes is a main immunological feature in patients with AIDS, but its basis are not well understood. It has been recently described that T and NK cell-mediated cytotoxicity can be regulated by HLA killer inhibitory receptors (KIR). In this work, we ha...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1999.00833.x
更新日期:1999-03-01 00:00:00
abstract::Secretion of IgM and IgG in vitro by B cells from patients with common variable immunodeficiency (CVI) has been used to classify the disease into three groups. On stimulation with anti-IgM and IL-2, group A patients' cells fail to secrete IgM or IgG, group B patients' cells secrete no IgG and significantly lower level...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1992.tb03020.x
更新日期:1992-03-01 00:00:00
abstract::The effect of normal human peripheral blood polymorphonuclear leucocytes on in vitro multiplication of Plasmodium falciparum malaria parasites was investigated. It was shown that normal neutrophils were able to phagocytose parasitized erythrocytes and free parasites and thus inhibit in vitro multiplication of the para...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract::Patients with Hodgkin's disease have increased numbers of spontaneously proliferating circulating lymphocytes. In order to test the relationship between this phenomenon and the in vitro mitogenic response, both spontaneous lymphocyte proliferation and the response to stimulation with phytohaemagglutinin were quantifie...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1978-04-01 00:00:00