Reduced mitogenic responses in cultures of lymphocytes from newly calved cows.

abstract：:Listeriolysin O (LLO) has been proposed as a potential carrier or adjuvant molecule in the vaccination field. However, the cytotoxic and pro-apoptotic effects of LLO are the major limitations for this purpose. Here, we have performed a preclinical safety evaluation and characterized a new potential adjuvant applicatio...

journal_title：Clinical and experimental immunology

authors： Hernández-Flores KG,Calderón-Garcidueñas AL,Mellado-Sánchez G,Ruiz-Ramos R,Sánchez-Vargas LA,Thomas-Dupont P,Izaguirre-Hernández IY,Téllez-Sosa J,Martínez-Barnetche J,Wood L,Paterson Y,Cedillo-Barrón L,López-Franco O,Vivanco-Ci

更新日期：2017-04-01 00:00:00

abstract：:Reliable risk assessment for biotherapeutics requires accurate evaluation of risk factors associated with immunogenicity. Immunogenicity risk assessment tools were developed and applied to investigate the immunogenicity of a fully human therapeutic monoclonal antibody, ATR-107 [anti-interleukin (IL)-21 receptor] that ...

journal_title：Clinical and experimental immunology

authors： Xue L,Hickling T,Song R,Nowak J,Rup B

更新日期：2016-01-01 00:00:00

abstract：:Complement induces mixed aggregation of murine spleen cells bearing C3 receptors. In the present studies, we show with immunofluorescent techniques that these aggregates are formed by T and B lymphocytes and myeloid cells, B cells constituting the predominant component in normal mice. There are striking changes in the...

journal_title：Clinical and experimental immunology

authors： Arnaiz-Villena A,Roitt IM

更新日期：1975-07-01 00:00:00

abstract：:Specific antibody deficiency (SAD) to unconjugated pneumococcal vaccine (PPV) is an established primary B cell immunodeficiency. The occurrence and natural history of SAD in children is unclear. We conducted an observational study to identify SAD in children with recurrent respiratory infections. Ninety-nine children,...

journal_title：Clinical and experimental immunology

authors： Ruuskanen O,Nurkka A,Helminen M,Viljanen MK,Käyhty H,Kainulainen L

更新日期：2013-05-01 00:00:00

abstract：:Circulating immune complexes (CIC) were detected by a solid-phase radioassay in 34% of fifty-three insulin-dependent diabetics (IDD) as compared to 18% of forty-five non-insulin-dependent diabetics (NIDD) and 14% of 173 control subjects. In control subjects, the prevalence of CIC increased with age and was higher in m...

journal_title：Clinical and experimental immunology

authors： Delespesse G,Gausset P,Sarfati M,Dubi-Rucquoy M,Debisschop MJ,van Haelst L

更新日期：1980-04-01 00:00:00

abstract：:Autoantibodies to the intermediate filament proteins vimentin and keratin were studied in sera of 50 Caribbean patients with Schistosoma mansoni infection and 50 control subjects. Autoantibodies were detected by indirect immunofluorescence on HEp-2 cells pretreated with colchicine. The incidence of anti-vimentin antib...

journal_title：Clinical and experimental immunology

authors： Boehme MW,Kataaha PK,Holborow EJ

更新日期：1989-08-01 00:00:00

abstract：:The depressed cell-mediated immunity in rheumatoid arthritis was investigated in vivo by cutaneous hypersensitivity responses to seven antigens including tuberculin PPD, and in vitro by lymphocyte transformation to the latter antigen. In vivo 40% of rheumatoid patients were anergic compared to 2% of controls (P less t...

journal_title：Clinical and experimental immunology

authors： Emery P,Panayi GS,Nouri AM

更新日期：1984-07-01 00:00:00

abstract：:Several patients with Paget's disease have been reported to have monoclonal IgM proteins in their serum. We have systematically studied 26 patients with Paget's disease severe enough to require diphosphonate therapy. Five of these patients were found to have isolated elevations of serum IgM which were well outside the...

journal_title：Clinical and experimental immunology

authors： Buxbaum JN,Kammerman S

更新日期：1984-04-01 00:00:00

abstract：:Anaemia of chronic disease (ACD) is frequently found in rheumatoid arthritis (RA). In the pathogenesis of ACD both cytokines, such as tumour necrosis factor-alpha (TNF-alpha), IL-1 and IL-6 as well as a relative deficiency of erythropoietin (EPO), are thought to play a key role. In the present study the role of IL-6 i...

journal_title：Clinical and experimental immunology

authors： Jongen-Lavrencic M,Peeters HR,Rozemuller H,Rombouts WJ,Martens AC,Vreugdenhil G,Pillay M,Cox PH,Bijser M,Brutel G,Breedveld FC,Swaak AJ

更新日期：1996-02-01 00:00:00

abstract：:Since thyroid follicular epithelial cells (thyrocytes) have been shown to express a number of functions similar to monocytes, they were further examined for their potency in secreting thymocyte-stimulating activity (TSA). Although spontaneous production of TSA could not be detected when thyrocytes were cultured in the...

journal_title：Clinical and experimental immunology

authors： Hirose W,Kawagoe M,Hara M,Kitani A,Hirose T,Norioka K,Harigai M,Nakamura H

更新日期：1987-10-01 00:00:00

abstract：:IgE rheumatoid factor activity was found to be significantly elevated (P less than 0.01) using an ELISA assay when paired synovial fluid and sera from 13 patients with active RA were compared to ten control samples. Synovial fluid IgE RF activity was higher than predicted by diffusion alone in 7/11 (64%) of the RA syn...

journal_title：Clinical and experimental immunology

authors： Gruber B,Ballan D,Gorevic PD

更新日期：1988-02-01 00:00:00

abstract：:Dendritic cells (DCs) were prepared from human bronchoalveolar lavage (BAL) cells. We previously reported that, in particular, the CD1a fraction of the low autofluorescent (LAF) cells contains the precursors for DCs: after overnight culture, 40% of the LAF cells change into functionally and phenotypically prototypic d...

journal_title：Clinical and experimental immunology

authors： Verhoeven GT,Van Haarst JM,De Wit HJ,Simons PJ,Hoogsteden HC,Drexhage HA

更新日期：2000-11-01 00:00:00

abstract：:T cells of C57BL/6J (B6) mice activated in vitro against the K and D determinants of the H-2d haplotype, and expanded in tissue culture in the presence of interleukin 2, were capable of rapidly killing lethally irradiated BALB/c recipients when injected intravenously. Mice given 10(6), 10(7) and 10(8) such T cells sur...

journal_title：Clinical and experimental immunology

authors： Andrus L,Atkinson K,Lafferty KJ

更新日期：1982-12-01 00:00:00

abstract：:We have developed CD4+, tetanus antigen-specific T cell clones that proliferate in the presence of tetanus antigen and autologous irradiated peripheral blood leucocytes (PBL) as antigen-presenting cells (APC). There have been several reports that T cells can present antigen themselves. We have used tetanus antigen-spe...

journal_title：Clinical and experimental immunology

authors： Chirmule N,Kalyanaraman VS,Slade H,Oyaizu N,Pahwa S

更新日期：1990-05-01 00:00:00

abstract：:Multiple halothane exposures in rabbits generate modified liver proteins or antigens that appear to incorporate the metabolic intermediate of halothane, trifluoroacetyl halide (TFA), as identified by specific anti-TFA antibody. These halothane-induced antigens are most prevalent throughout the second to fourth days fo...

journal_title：Clinical and experimental immunology

authors： Roth TP,Hubbard AK,Gandolfi AJ,Brown BR

更新日期：1988-05-01 00:00:00

abstract：:In order to evaluate the impact of induction and increase target antigen expression on immunotoxin potency, we measured the potentiating effect of recombinant immune interferon-gamma (rIFN-gamma) on the cytotoxicity of an anti HLA-DR ricin A-chain immunotoxin (2G5 RTA-IT) on the myeloid cell line ML-3. After 48 h of i...

journal_title：Clinical and experimental immunology

authors： Chiron M,Jaffrezou JP,Carayon P,Bordier C,Roubinet F,Xavier C,Brandely M,Laurent G

更新日期：1990-11-01 00:00:00

abstract：:Monocytes (MO) migrating into normal, non-inflamed intestinal mucosa undergo a specific differentiation resulting in a non-reactive, tolerogenic intestinal macrophage (IMAC). Recently we demonstrated the differentiation of MO into an intestinal-like macrophage (MAC) phenotype in vitro in a three-dimensional cell cultu...

journal_title：Clinical and experimental immunology

authors： Spoettl T,Hausmann M,Herlyn M,Gunckel M,Dirmeier A,Falk W,Herfarth H,Schoelmerich J,Rogler G

更新日期：2006-07-01 00:00:00

abstract：:Epstein-Barr virus (EBV) is a persistent virus with oncogenic capacity that has been implicated in the development of aggressive B cell lymphomas, primarily in immunosuppressed individuals, although it can be present in immunocompetent individuals. Changes in the function and clonal diversity of T lymphocytes might be...

journal_title：Clinical and experimental immunology

authors： Cárdenas D,Vélez G,Orfao A,Herrera MV,Solano J,Olaya M,Uribe AM,Saavedra C,Duarte M,Rodríguez M,López M,Fiorentino S,Quijano S

更新日期：2015-11-01 00:00:00

abstract：:Human peripheral blood B lymphocytes, when cultured for 6 days with ovalbumin in the presence of T cells and monocytes, can differentiate into plaque-forming cells (PFC) secreting small amounts of antigen-specific IgM. Phenotype analysis using Ig-isotype and B-cell specific monoclonal antibodies revealed that the PFC ...

journal_title：Clinical and experimental immunology

authors： Hart LA,Heijnen CJ,Zijlstra J,Ballieux RE

更新日期：1985-09-01 00:00:00

abstract：:Bovine type II collagen administered to non-pregnant female DBA/1 mice caused arthritis in 55% of animals with a mean onset time of 70 days following immunization. Of collagen-treated females subsequently becoming syngeneically pregnant before the onset of arthritis, all developed the disease within 10 days of parturi...

journal_title：Clinical and experimental immunology

authors： Waites GT,Whyte A

更新日期：1987-03-01 00:00:00

abstract：:Peripheral large granular lymphocytes (LGL) were enumerated in normal subjects and patients with autoimmune thyroid diseases. In normal subjects, the percentage of LGL was significantly lower in women (mean +/- s.d., 17.0 +/- 3.6%; n = 35; P less than 0.05) than in men (19.5 +/- 5.3%; n = 20). In untreated patients wi...

journal_title：Clinical and experimental immunology

authors： Iwatani Y,Amino N,Kabutomori O,Mori H,Tamaki H,Motoi S,Izumiguchi Y,Miyai K

更新日期：1984-01-01 00:00:00

abstract：:Monocyte-depleted mononuclear cells (MNC) from healthy subjects synthesized IgM-class antibody to single-stranded DNA (anti-ssDNA) at significantly higher levels than unfractionated MNC. The increased production of IgM anti-ssDNA by monocyte-depleted normal MNC was inhibited to a greater degree than total IgM by addit...

journal_title：Clinical and experimental immunology

authors： Okawa-Takatsuji M,Aotsuka S,Uwatoko S,Kinoshita M,Yokohari R

更新日期：1989-09-01 00:00:00

abstract：:Incubation with thymosin fraction 5, (TMS F5 at 300 micrograms/ml) a partially purified thymic factor, reduced the steroid binding activity of human infant thymocytes from 9.6 +/- 2.1 fmole/ml to 5.0 +/- 2.0 fmole/ml. The glucocorticoid receptor activity in normal infant thymocytes was found to be 2,146 +/- 726 (s.d.)...

journal_title：Clinical and experimental immunology

authors： Ma DD,Ho AH,Hoffbrand AV

更新日期：1984-02-01 00:00:00

abstract：:Natural killer (NK) cytotoxic activity was simultaneously investigated in bone marrow mononuclear cells (BMMC) and peripheral blood lymphocytes (PBL) from nine Hodgkin's disease (HD) and 15 non-Hodgkin lymphoma (NHL) untreated patients. Twenty-five PBL samples and seven bone marrow specimens from healthy individuals w...

journal_title：Clinical and experimental immunology

authors： Caldera LH,Leon-Ponte M,Acquatella G,Bianco NE,Blanca I

更新日期：1992-04-01 00:00:00

abstract：:Recent evidence has shown that microRNA-126 (miR-126) has been involved in the development and function of immune cells, which contributed to the pathogenesis of related clinical diseases. However, the potential role of miR-126 in the development and function of CD4+ T cells remains largely unknown. Here we first foun...

journal_title：Clinical and experimental immunology

authors： Chu F,Hu Y,Zhou Y,Guo M,Lu J,Zheng W,Xu H,Zhao J,Xu L

更新日期：2018-02-01 00:00:00

abstract：:The levels of soluble IL-2Ralpha (sIL-2Ralpha) in serum were measured in HTLV-1 carriers and ATL patients in order to evaluate their possible correlation with clinical status. Mean sIL-2R levels in ATL patients were found to be 9704 U/ml for the acute/lymphoma type, 1961 U/ml for the chronic type and 788 U/ml for the ...

journal_title：Clinical and experimental immunology

authors： Araki K,Harada K,Nakamoto K,Shiroma M,Miyakuni T

更新日期：2000-02-01 00:00:00

abstract：:Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharides, plays an important role in the innate immune response. In this study, we investigated the role of TLR4 in the development of experimental colitis with regard to the biological actions of macrophage migration inhibitory factor (MIF) using TLR4 null ((-/...

journal_title：Clinical and experimental immunology

authors： Ohkawara T,Takeda H,Nishihira J,Miyashita K,Nihiwaki M,Ishiguro Y,Takeda K,Akira S,Iwanaga T,Sugiyama T,Asaka M

更新日期：2005-09-01 00:00:00

abstract：:The capacity of B cells from patients with chronic lymphocytic leukaemia (CLL) to produce and secrete immunoglobulin following mitogen stimulation was investigated using sensitive radioimmunoassays for mu, gamma, alpha, kappa, and lambda immunoglobulin chains. Lymphocytes from seven of the 11 patients studied secreted...

journal_title：Clinical and experimental immunology

authors： Johnstone AP,Jensenius JC,Millard RE,Hudson L

更新日期：1982-03-01 00:00:00

abstract：:Thyroid peroxidase purified from porcine thyroid (pTPO) was found to induce an experimental murine thyroiditis with genetic restriction which was very different from that induced by mouse thyroglobulin (mTg). C57BL/6 and C57BL/10 (both H-2b) were good responders for thyroiditis, whereas A/J (H-2a), BALB/c (H-2d), DBA/...

journal_title：Clinical and experimental immunology

authors： Kotani T,Umeki K,Hirai K,Ohtaki S

更新日期：1990-04-01 00:00:00

abstract：:Earlier data suggest that gamma/delta T cells may play an important role in the immune response to Mycobacterium tuberculosis. The aim of this study was to determine the percentage of different gamma/delta subsets in peripheral blood of active tuberculosis patients with a positive or negative tuberculin reaction. Thir...

journal_title：Clinical and experimental immunology

authors： Szereday L,Baliko Z,Szekeres-Bartho J

更新日期：2003-02-01 00:00:00