Insulin autoantibodies with high affinity to the bovine milk protein alpha casein.


:Insulin autoantibodies (IAA) can appear in children within months of introducing solid foods to the diet and before clinical type 1 diabetes. The aim of this study was to determine whether infant dietary antigens could be immunizing agents of IAA. To this end, IAA binding to [(125) I]insulin was competed with food preparations and extracts of foods encountered in the infant diet (milk formulas, bovine milk, wheat flour, fowl meal). Bovine milk powder extracts inhibited IAA-positive samples from six of 53 children (age 0·3-14·0 years) participating in German prospective cohorts. Inhibition in these sera ranged from 23 to 100%. Competition was abolished when hydrolyzed milk powder was used. Competition with protein components of bovine milk found that two of the six milk-reactive sera were inhibited strongly by alpha- and beta-casein; none were inhibited by the milk proteins bovine serum albumin or lactoglobulins. The two casein-reactive sera had high affinity to alpha-casein (1·7×10(9) ; 3·1×10(9)  l/mol), and lesser affinity to beta-casein (4·0×10(8) ; 7·0×10(7)  l/mol) and insulin (2·6×10(8) ; 1·6×10(8)  l/mol). No children with milk-reactive IAA developed autoantibodies to other islet autoantigens or diabetes (median follow-up 9·8 years). These results suggest that autoimmunity to insulin can occur infrequently via cross-reactivity to food proteins, but this form of IAA immunization does not appear to be associated with progression to diabetes.


Clin Exp Immunol


Adler K,Mueller DB,Achenbach P,Krause S,Heninger AK,Ziegler AG,Bonifacio E




Has Abstract


2011-04-01 00:00:00












  • C1-inhibitor efficiently inhibits Escherichia coli-induced tissue factor mRNA up-regulation, monocyte tissue factor expression and coagulation activation in human whole blood.

    abstract::Both the complement system and tissue factor (TF), a key initiating component of coagulation, are activated in sepsis, and cross-talk occurs between the complement and coagulation systems. C1-inhibitor (C1-INH) can act as a regulator in both systems. Our aim in this study was to examine this cross-talk by investigatin...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Landsem A,Nielsen EW,Fure H,Christiansen D,Ludviksen JK,Lambris JD,Østerud B,Mollnes TE,Brekke OL

    更新日期:2013-08-01 00:00:00

  • Sequential autoantigenic determinants of the small nuclear ribonucleoprotein Sm D shared by human lupus autoantibodies and MRL lpr/lpr antibodies.

    abstract::Autoantibodies directed against the Sm proteins of the spliceosome complex are found in approximately 25% of systemic lupus erythematosus (SLE) patients sera. To determine which regions of the Sm D polypeptide are involved in the lupus autoimmune response, binding to overlapping octapeptides of Sm D has been evaluated...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: James JA,Mamula MJ,Harley JB

    更新日期:1994-12-01 00:00:00

  • Kinetic properties of 5' nucleotidase in blood lymphocytes from healthy subjects, immunodeficient patients and cord blood.

    abstract::The Km for AMP for 5' nucleotidase was increased in lymphocytes from patients with common variable (CVH) and sex linked (XLH) hypogammaglobulinaemia and from patients with chronic lymphatic leukaemia (CLL): the Vmax of the latter was low. The kinetic constants for cord blood lymphocytes are similar to adults and the K...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Shah T,Webster AD,Peters TJ

    更新日期:1984-07-01 00:00:00

  • The role of the immune system in kidney disease.

    abstract::The immune system and the kidneys are closely linked. In health the kidneys contribute to immune homeostasis, while components of the immune system mediate many acute forms of renal disease and play a central role in progression of chronic kidney disease. A dysregulated immune system can have either direct or indirect...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Tecklenborg J,Clayton D,Siebert S,Coley SM

    更新日期:2018-05-01 00:00:00

  • Differential activation of the immune system by virulent Streptococcus pneumoniae strains determines recovery or death of the host.

    abstract::Streptococcus pneumoniae infection may result in asymptomatic carriage, mucosal or invasive disease. We hypothesize that self-limiting or fatal disease outcome follows infection with S. pneumoniae differential activation of the host immune response. BALB/c and C57BL/6 mice were inoculated intranasally with S. pneumoni...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Mizrachi-Nebenzahl Y,Lifshitz S,Teitelbaum R,Novick S,Levi A,Benharroch D,Ling E,Dagan R

    更新日期:2003-10-01 00:00:00

  • Immunology in clinic review series; focus on autoinflammatory diseases: inflammasomes: mechanisms of activation.

    abstract:UNLABELLED:OTHER THEMES PUBLISHED IN THIS IMMUNOLOGY IN THE CLINIC REVIEW SERIES Allergy, Host Responses, Cancer, Type 1 diabetes and viruses, Metabolic diseases. SUMMARY:Initiation of a successful immune response requires a working set of sensors that detect any noxious agent within the cellular microenvironment and ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Mankan AK,Kubarenko A,Hornung V

    更新日期:2012-03-01 00:00:00

  • Variable effects of cyclophosphamide in rodent models of experimental allergic encephalomyelitis.

    abstract::In this study, we have evaluated the effects of cyclophosphamide on the development of experimental allergic encephalomyelitis (EAE) in four EAE rodent models: monophasic EAE in Lewis rats, protracted relapsing (PR)-EAE in DA rats, myelin oligodendrocyte protein (MOG)-induced EAE in C57Bl/6 mice and proteolipid protei...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Mangano K,Nicoletti A,Patti F,Donia M,Malaguarnera L,Signorelli S,Magro G,Muzio V,Greco B,Zaratin P,Meroni P,Zappia M,Nicoletti F

    更新日期:2010-02-01 00:00:00

  • Impaired natural killer cell-induced antibody-dependent cell-mediated cytotoxicity is associated with human immunodeficiency virus-1 disease progression.

    abstract::This study evaluates the correlation between natural killer (NK) cell function and human immunodeficiency virus (HIV)-1 disease progression in 133 untreated HIV-1 positive Chinese subjects, including 41 former plasma donors (FPDs) and 92 men who have sex with men, and 35 HIV-negative controls. Flow cytometry was used ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Jia M,Li D,He X,Zhao Y,Peng H,Ma P,Hong K,Liang H,Shao Y

    更新日期:2013-01-01 00:00:00

  • Proliferation and production of interferon-gamma (IFN-gamma) and IL-4 in response to Staphylococcus aureus and staphylococcal superantigen in childhood atopic dermatitis.

    abstract::We have examined the cell-mediated immunity (CMI) to Staphylococcus aureus (S. aureus) and Staphylococcal enterotoxin B (SEB) in peripheral blood mononuclear cells (PBMC) from children with atopic dermatitis (AD) and from non-atopic child controls by measurement of proliferative responses and production of the cytokin...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Campbell DE,Kemp AS

    更新日期:1997-02-01 00:00:00

  • Antibody-dependent and phytohaemagglutinin-induced lymphocyte cytotoxicity in systemic sclerosis.

    abstract::Cell-mediated cytotoxicity was examined in thirty-seven patients with systemic sclerosis using both whole blood and purified peripheral blood mononuclear cells (PBM) to measure antibody-dependent (ADCC) and phytohaemagglutinin (PHA) induced lymphocyte cytotoxicity to 51Cr-labelled Chang liver cells. In twenty-three mi...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Wright JK,Hughes P,Rowell NR,Sneddon IB

    更新日期:1979-04-01 00:00:00

  • Demonstration of increased anti-mycobacterial activity in peripheral blood monocytes after BCG vaccination in British school children.

    abstract::A blood sample was taken from children aged 13-15 years immediately before BCG vaccination and 8 weeks after. The children were tuberculin skin-test negative to PPD-S before vaccination and positive after. Mononuclear cells were separated from the blood, infected with Mycobacterium microti at a low bacterium/monocyte ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Cheng SH,Walker L,Poole J,Aber VR,Walker KB,Mitchison DA,Lowrie DB

    更新日期:1988-10-01 00:00:00

  • Characterization of immunological depression in mice exposed to normobaric oxygen.

    abstract::Immunological cell functions were evaluated during 24, 48 and 96 h O2 exposure in C57Bl/6 mice. A normobaric O2 exposure resulted in depression of delayed type hypersensitivity (DTH) to oxazolone and Staphylococcus aureus antigens. This effect was proportional to the duration of O2 exposure. The antibody response of s...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Levacher-Place M,Gougerot-Pocidalo MA,Rouveix B,Kraus L,Pocidalo JJ

    更新日期:1983-11-01 00:00:00

  • Generation of interleukin-2-dependent T cell lines from synovial fluids in rheumatoid arthritis.

    abstract::Synovial fluids from rheumatoid arthritis (RA) patients were found to contain activated T lymphocytes that could be maintained as continuous T cell lines (CTCL) in the presence of the T cell growth factor, interleukin (IL)-2. The CTCL predominantly expressed the OKT8 phenotype and were Ia antigen positive. IL-2-depend...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Wilkins JA,Olivier SL,Warrington RJ

    更新日期:1984-10-01 00:00:00

  • Enhancement of the lytic activity of cloned human CD8 tumour-infiltrating lymphocytes by bispecific monoclonal antibodies.

    abstract::We have isolated tumour-infiltrating lymphocytes (TIL) clones from a patient with renal cell cancer. The cloning frequency and the effector function were measured. No difference in cloning frequency (r2 = 0.97, frequency = 1:13) was observed between TIL expanded with allogeneic versus autologous feeder cells. Sixty-fo...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Gorter A,Krüse KM,Schrier PI,Fleuren GJ,van de Griend RJ

    更新日期:1992-01-01 00:00:00

  • Apoptosis of Th1-like cells in experimental tuberculosis (TB).

    abstract::Th1 cell-induced anti-mycobacterial immunity is lost during a progressive Mycobacterium tuberculosis infection in a susceptible host. This study was designed to test the mechanism of the loss of anti-mycobacterial cell-mediated immune response. We demonstrate that M. tuberculosis infection results in increased Fas exp...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Das G,Vohra H,Saha B,Agrewala JN,Mishra GC

    更新日期:1999-02-01 00:00:00

  • Anti-cardiolipin antibodies and circulating immune complexes in type 1 diabetes mellitus: increased prevalence and relation to vascular complications.

    abstract::Anti-cardiolipin antibodies, oxidatively modified low-density lipoproteins (oxLDL) and circulating immune complexes are humoral factors that have been linked to vascular damage. To analyse their possible role in the vascular complications in type 1 diabetes mellitus, we investigated patients with and without vascular ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ahmed E,Nityanand S,Mustafa A,Brismar K,Lefvert AK

    更新日期:1999-02-01 00:00:00

  • Impaired activation of Stat1 and c-Jun as a possible defect in macrophages of patients with active tuberculosis.

    abstract::Studies of patients with active tuberculosis (TB) and infected healthy individuals have shown that interferon (IFN)-gamma is present in sites of Mycobacterium tuberculosis infection in comparable levels. This suggests that there is a deficiency in the macrophage response to IFN-gamma in TB patients. We used recombinan...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Esquivel-Solís H,Quiñones-Falconi F,Zarain-Herzberg A,Amieva-Fernández RI,López-Vidal Y

    更新日期:2009-10-01 00:00:00

  • Elevated expression of CCR5 by myeloid (CD11c+) blood dendritic cells in multiple sclerosis and acute optic neuritis.

    abstract::Myeloid and plasmacytoid dendritic cells (DC) are present in cerebrospinal fluid (CSF) in non-inflammatory neurological diseases (NIND) and elevated in clinically definite multiple sclerosis (MS) and in early MS - acute monosymptomatic optic neuritis (ON). Here, we show that expression of CCR5, a chemokine receptor fo...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Pashenkov M,Teleshova N,Kouwenhoven M,Kostulas V,Huang YM,Soderstrom M,Link H

    更新日期:2002-03-01 00:00:00

  • Depletion of CD4+ CD25+ regulatory T cells inhibits local tumour growth in a mouse model of B cell lymphoma.

    abstract::Regulatory T cells (T(regs)) may inhibit immunity against cancer. Induction and expansion of T(regs) in the immunosuppressive microenvironment created by a growing tumour appear to be one of the mechanisms by which it can evade host defence. We studied the impact of CD25+ T(regs) in a B cell lymphoma model in which Ra...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Heier I,Hofgaard PO,Brandtzaeg P,Jahnsen FL,Karlsson M

    更新日期:2008-05-01 00:00:00

  • IgG-mediated anaphylaxis via Fc gamma receptor in CD40-deficient mice.

    abstract::Anaphylaxis denotes an immediate hypersensitivity reaction to allergen, exclusively mediated by IgE antibodies. However, IgE antibodies do not explain all the syndromes that are encountered. We investigated potent IgG-mediated anaphylaxis in CD40-deficient mice that lack the immunoglobulin class switching for T cell-d...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Wakayama H,Hasegawa Y,Kawabe T,Saito H,Kikutani H,Shimokata K

    更新日期:1998-11-01 00:00:00

  • The Italian quality control study for evaluation of CD4 cells in centres involved in the treatment of HIV-1 patients. Italian CD4 Quality Control Group.

    abstract::We report on the experience of establishing a national network for a quality control programme in evaluating CD4 cell counts in most Italian centres involved in the care of patients with HIV disease. The 68 centres were divided according to their geographical location into eight groups, and twice a year (tests A and B...

    journal_title:Clinical and experimental immunology

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Pandolfi F,Alario C,Girardi E,Rava L,Ippolito G,Kunkl A,Aiuti F

    更新日期:1998-03-01 00:00:00

  • Failure of Mycobacterium leprae soluble antigens to suppress delayed-type hypersensitivity reaction to tuberculin.

    abstract::In order to test a published claim that the inclusion of Mycobacterium leprae antigens with a tuberculin skin test reagent can suppress delayed-type hypersensitivity (DTH) to tuberculin in both paucibacillary and multibacillary leprosy cases, 109 leprosy cases and 104 non-leprosy controls were skin-tested simultaneous...

    journal_title:Clinical and experimental immunology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Fine PE,Gruer PJ,Maine N,Ponnighaus JM,Rees RJ,Stanford JL

    更新日期:1989-08-01 00:00:00

  • Lymphocytotoxins and mixed leucocyte culture blocking factor activity in the plasma of uraemic patients undergoing haemodialysis.

    abstract::Mixed leucocyte culture blocking factor activity (MLC-BFA) in the plasma of haemodialysis patients appears as a result of recent blood transfusions and is concentrated in IgG fractions of the blocking serum. Four patients neither developed lymphocytotoxins nor MLC-BFA in spite of having received multiple blood transfu...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Sengar DP,Rashid A,Perelmutter L,Harris JE

    更新日期:1975-05-01 00:00:00

  • Avidity of thyroglobulin antibody in sera from patients with Hashimoto's thyroiditis with different thyroid functional status.

    abstract::The mechanism of disease progression in Hashimoto's thyroiditis (HT) is still unclear. Thyroglobulin antibody (TgAb) is a diagnostic hallmark of HT. The aim of our study was to evaluate the avidity of TgAb in sera from HT patients with different thyroid functional status. Sera from 50 patients with newly diagnosed HT ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Zhang Y,Gao Y,Li M,Xie L,Huang Y,Gao Y,Guo X

    更新日期:2010-07-01 00:00:00

  • Functional T lymphocytes infiltrate implanted polyvinyl alcohol foams during surgical wound closure therapy.

    abstract::Vacuum-assisted closure involving the implantation of polyvinyl alcohol foam is a technique recently developed for the treatment of patients suffering from either wound infection or chronic wounds. This method has been shown to improve and accelerate wound healing. However, little is known about the cell populations t...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Gouttefangeas C,Eberle M,Ruck P,Stark M,Müller JE,Becker HD,Rammensee HG,Pinocy J

    更新日期:2001-06-01 00:00:00

  • Monocyte function in Hodgkin's disease.

    abstract::Monocyte functions were studied in 16 patients with Hodgkin's disease, 11 untreated and five in unmaintained complete remission. Eleven untreated patients with non-Hodgkin's lymphomas and 21 healthy persons were used as controls. Monocytes were isolated from peripheral blood and enriched to greater than 90%. Lymphoma ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Holm G,Björkholm M,Johansson B,Mellstedt H,Lindemalm C

    更新日期:1982-01-01 00:00:00

  • A human suppressor lymphocyte subpopulation identified by a heteroantiserum.

    abstract::A rabbit antiserum has been prepared using peripheral mononuclear cells from a patient with common variable hypogammaglobulinaemia (CVH) who had demonstrable suppressor cells in vitro. This antiserum (anti-Ck), after absorption with pooled normal glass-non-adherent cells and platelets, showed selective reactivity with...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Rizzoli AJ,Bankhurst AD

    更新日期:1981-12-01 00:00:00

  • Autoantibodies to crude human leucocyte interferon (IFN), native human IFN, recombinant human IFN-alpha 2b and human IFN-gamma in healthy blood donors.

    abstract::Recently, naturally occurring antibodies to IFN-alpha were discovered in a few systemic lupus erythematosus (SLE) and cancer patients; however, in most patients monitored for anti-IFN antibodies before treatment, no antibodies were found. In an attempt to explain the 'IFN-blocking effect' that we observed in all serum...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ross C,Hansen MB,Schyberg T,Berg K

    更新日期:1990-10-01 00:00:00

  • In vitro and in vivo functional analysis of CD5+ and CD5- B cells of autoimmune NZB x NZW F1 mice.

    abstract::Polyclonal B cell activation has been thought to play the critical role in production of autoantibodies, and possible activation of autoreactive T cells in murine lupus, especially abnormal expansion of CD5+ B cells, is one of the characteristic findings in these mice. The aim of this study was to investigate further ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ye YL,Chuang YH,Chiang BL

    更新日期:1996-11-01 00:00:00

  • Lung cell responses to M. tuberculosis in genetically susceptible and resistant mice following intratracheal challenge.

    abstract::One approach to study the role of distinct cellular mechanisms in susceptibility/resistance to tuberculosis (TB) is to compare parameters of response to infection in the lungs of mouse strains exhibiting genetically determined differences in TB susceptibility/severity. Interstrain differences in antimycobacterial macr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Eruslanov EB,Majorov KB,Orlova MO,Mischenko VV,Kondratieva TK,Apt AS,Lyadova IV

    更新日期:2004-01-01 00:00:00