Insulin autoantibodies with high affinity to the bovine milk protein alpha casein.


:Insulin autoantibodies (IAA) can appear in children within months of introducing solid foods to the diet and before clinical type 1 diabetes. The aim of this study was to determine whether infant dietary antigens could be immunizing agents of IAA. To this end, IAA binding to [(125) I]insulin was competed with food preparations and extracts of foods encountered in the infant diet (milk formulas, bovine milk, wheat flour, fowl meal). Bovine milk powder extracts inhibited IAA-positive samples from six of 53 children (age 0·3-14·0 years) participating in German prospective cohorts. Inhibition in these sera ranged from 23 to 100%. Competition was abolished when hydrolyzed milk powder was used. Competition with protein components of bovine milk found that two of the six milk-reactive sera were inhibited strongly by alpha- and beta-casein; none were inhibited by the milk proteins bovine serum albumin or lactoglobulins. The two casein-reactive sera had high affinity to alpha-casein (1·7×10(9) ; 3·1×10(9)  l/mol), and lesser affinity to beta-casein (4·0×10(8) ; 7·0×10(7)  l/mol) and insulin (2·6×10(8) ; 1·6×10(8)  l/mol). No children with milk-reactive IAA developed autoantibodies to other islet autoantigens or diabetes (median follow-up 9·8 years). These results suggest that autoimmunity to insulin can occur infrequently via cross-reactivity to food proteins, but this form of IAA immunization does not appear to be associated with progression to diabetes.


Clin Exp Immunol


Adler K,Mueller DB,Achenbach P,Krause S,Heninger AK,Ziegler AG,Bonifacio E




Has Abstract


2011-04-01 00:00:00












  • Clinical significance of serum soluble IL-2R levels in patients with adult T cell leukaemia (ATL) and HTLV-1 carriers.

    abstract::The levels of soluble IL-2Ralpha (sIL-2Ralpha) in serum were measured in HTLV-1 carriers and ATL patients in order to evaluate their possible correlation with clinical status. Mean sIL-2R levels in ATL patients were found to be 9704 U/ml for the acute/lymphoma type, 1961 U/ml for the chronic type and 788 U/ml for the ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Araki K,Harada K,Nakamoto K,Shiroma M,Miyakuni T

    更新日期:2000-02-01 00:00:00

  • Stimulation of antibody synthesis induced by surgical trauma in rats.

    abstract::The effect of a standard laparatomy on antibody synthesis was studied in Wistar R/A rats recieving an intravenous injection of 10(9) sheep red blood cells (SRBC) during the surgical procedure. Anti-SRBC antibody titres were significantly higher in operated animals than in controls. When SRBC were given 2 hr after the ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kinnaert P,Mahieu A,Van Geertruyden N

    更新日期:1978-05-01 00:00:00

  • Immunosuppression by sex steroid hormones. The effect upon PHA- and PPD-stimulated lymphocytes.

    abstract::Progesterone, estradiol, testosterone, cortisol, and 11-desoxycortisol (compound S) were added to cultures of human lymphocytes stimulated with phytohaemagglutinin (PHA) and purified protein derivative (PPD). The immunosuppressive effect of cortisol was verified and the three sex-steroid hormones also were found to in...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Wyle FA,Kent JR

    更新日期:1977-03-01 00:00:00

  • Association between mannan-binding lectin and impaired lung function in cystic fibrosis may be age-dependent.

    abstract::An association between mannan-binding lectin (MBL) status and severity of lung function impairment in cystic fibrosis (CF) has been found in several studies, but not in others. To explore the possible basis for discrepancies in the literature, we related both MBL and L-ficolin concentrations to lung function and exami...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Muhlebach MS,MacDonald SL,Button B,Hubbard JJ,Turner ML,Boucher RC,Kilpatrick DC

    更新日期:2006-08-01 00:00:00

  • Tissue factor over-expression in platelets of patients with anti-phospholipid syndrome: induction role of anti-β2-GPI antibodies.

    abstract::Anti-phospholipid syndrome (APS) is characterized by arterial and/or venous thrombosis and pregnancy morbidity. It is well known that in these patients thrombosis may be the result of a hypercoagulable state related to anti-β2-glycoprotein I (β2-GPI) antibodies. Moreover, platelets may play a role in thrombotic manife...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Capozzi A,Manganelli V,Riitano G,Recalchi S,Truglia S,Alessandri C,Longo A,Garofalo T,Misasi R,Valesini G,Conti F,Sorice M

    更新日期:2019-04-01 00:00:00

  • IgE induces secretion of prostaglandin E2 by human monocytes.

    abstract::IgE was isolated from a patient with the hyper IgE, recurrent infection syndrome by immunoadsorption on sepharose bound goat anti-human IgE. Addition of this IgE to a monolayer culture of human monocytes resulted in a dose-dependent increase in PGE2 secretion. The addition of F(ab')2 fraction of goat anti-human IgE in...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Passwell JH,Geiger B,Levanon M,Davidsohn J,Cohen BE,Ramot B

    更新日期:1984-12-01 00:00:00

  • Anti-collagen antibodies in systemic sclerosis and in primary Raynaud's phenomenon.

    abstract::The frequency and specificity of antibodies to native and denatured collagens were evaluated in systemic sclerosis (SSc) and in primary Raynaud's phenomenon (PRP) by direct and competitive ELISA. Antibodies reactive with denatured collagen type I (CI) were found in 43% of the SSc sera, and anti-CIV and anti-CV in 31%....

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Riente L,Marchini B,Dolcher MP,Puccetti A,Bombardieri S,Migliorini P

    更新日期:1995-11-01 00:00:00

  • T cell participation in autoreactivity to NC16a epitopes in bullous pemphigoid.

    abstract::Bullous pemphigoid is a blistering skin disease characterized by autoantibodies against the NC16a domain of bullous pemphigoid 180. This study was performed to characterize and map the fine specificity of T cell responses to NC16a. Peripheral blood mononuclear cells (PBMC) from a total of 28 bullous pemphigoid patient...

    journal_title:Clinical and experimental immunology

    pub_type: 临床试验,杂志文章


    authors: Pickford WJ,Gudi V,Haggart AM,Lewis BJ,Herriot R,Barker RN,Ormerod AD

    更新日期:2015-05-01 00:00:00

  • Increased expression of class II antigens of the major histocompatibility complex on alveolar macrophages and alveolar type II cells and interleukin-1 (IL-1) secretion from alveolar macrophages in an animal model of silicosis.

    abstract::Silicosis is a chronic progressive granulomatous and fibrotic lung disease caused by inhaled silica. Although the causative agent is known, the pathogenesis, especially the immunologic response, is not well understood. We examined two important components of cell-mediated immune responses in the lungs of rats with sil...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Struhar DJ,Harbeck RJ,Gegen N,Kawada H,Mason RJ

    更新日期:1989-08-01 00:00:00

  • Localization of 20-kD homologous restriction factor (HRF20) in diseased human glomeruli. An immunofluorescence study.

    abstract::The 20-kD homologous restriction factor (HRF20), which is identical to CD59, is a membrane-associated protein which inhibits the reaction of C9 to form membrane attack complex (MAC) of homologous complements. In various human glomerular diseases deposition of complement components is frequently seen and MAC is reporte...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Tamai H,Matsuo S,Fukatsu A,Nishikawa K,Sakamoto N,Yoshioka K,Okada N,Okada H

    更新日期:1991-05-01 00:00:00

  • Tumour necrosis factor-alpha and interferon-gamma production measured at the single cell level in normal and inflamed human intestine.

    abstract::The spot-ELISA technique has been used to enumerate the frequency of cells secreting tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma), isolated from biopsies of normal intestine and from biopsies of children with inflammatory bowel disease. TNF-alpha production was undetectable in six out of 12...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: MacDonald TT,Hutchings P,Choy MY,Murch S,Cooke A

    更新日期:1990-08-01 00:00:00

  • Heat shock protein peptides reactive in patients with Behçet's disease are uveitogenic in Lewis rats.

    abstract::Mycobacterial and homologous human heat shock protein T cell peptide epitopes specific for T lymphocytes in Behçet's disease were investigated for their pathogenicity in Lewis rats. The potential pathogenicity of eight peptides and two controls was assessed by administering the peptides in enriched Freund's adjuvant i...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Stanford MR,Kasp E,Whiston R,Hasan A,Todryk S,Shinnick T,Mizushima Y,Dumonde DC,van der Zee R,Lehner T

    更新日期:1994-08-01 00:00:00

  • Induction of unresponsiveness against IgA in IgA-deficient patients on subcutaneous immunoglobulin infusion therapy.

    abstract::Patients with IgA deficiency often demonstrate circulating antibodies against IgA, which have been suggested to be associated with transfusion reactions. Sera from three patients with common variable immunodeficiency (CVID) and one with a selective IgA deficiency with anti-IgA antibodies receiving subcutaneous gammagl...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Sundin U,Nava S,Hammarström L

    更新日期:1998-05-01 00:00:00

  • Natural killer (NK) T cells are significantly decreased in the peripheral blood of patients with rheumatoid arthritis (RA).

    abstract::The number of NK T cells was measured in relation to the Th1/Th2 imbalance observed in RA. Peripheral blood samples of patients with RA (n = 60) and healthy controls (n = 36) were stained with anti-NK receptor 1A (anti-NKR-P1A), anti-CD56, and anti-CD3 MoAbs, and examined by three-colour flow cytometry. NK T (NKR-P1A+...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Yanagihara Y,Shiozawa K,Takai M,Kyogoku M,Shiozawa S

    更新日期:1999-10-01 00:00:00

  • Immune-bone interplay in the structural damage in rheumatoid arthritis.

    abstract::The immune and bone systems maintain homeostasis by interacting closely with each other. Rheumatoid arthritis is a pathological consequence of their interplay, as activated T cell immune responses result in osteoclast-mediated bone erosion. An imbalance between forkhead box protein 3 (Foxp3)+ regulatory T (Treg ) cell...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Komatsu N,Takayanagi H

    更新日期:2018-10-01 00:00:00

  • Immunization against Plasmodium falciparum asexual blood stages using soluble antigens.

    abstract::Saimiri sciureus monkeys have been successfully immunized against a human malaria parasite, Plasmodium falciparum, using soluble antigens purified from schizont and merozoite extracts. High levels of antibodies reacting with schizont and merozoite specific polypeptides of 140 and 200 kdaltons were detected in the sera...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Perrin LH,Loche M,Dedet JP,Roussilhon C,Fandeur T

    更新日期:1984-04-01 00:00:00

  • Alterations in mononuclear cell tumour necrosis factor-alpha (TNF-alpha) response in patients on long term cuprophane haemodialysis.

    abstract::We have investigated TNF-alpha secretory response of peripheral blood mononuclear cells (PBMC) from 13 uraemic patients undergoing regular haemodialysis with cuprophane membrane (CM). Sixteen healthy subjects and five uraemic patients under conservative therapy were also studied as controls. Cells of haemodialysis pat...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Annenkov AY,Strokov AG,Baranova FS

    更新日期:1992-10-01 00:00:00

  • A novel point mutation in CD18 causing the expression of dysfunctional CD11/CD18 leucocyte integrins in a patient with leucocyte adhesion deficiency (LAD).

    abstract::Leucocyte adhesion deficiency type 1 (LAD-1) is characterized by the incapacity of leucocytes to carry out their adhesion functions via their CD11/CD18 antigens, which are also referred to as the leucocyte integrins. The patients generally suffer from poor wound healing and recurrent bacterial and fungal infections. I...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Mathew EC,Shaw JM,Bonilla FA,Law SK,Wright DA

    更新日期:2000-07-01 00:00:00

  • Invasive amoebiasis is associated with the development of anti-neutrophil cytoplasmic antibody.

    abstract::Features of tissue damage in invasive amoebiasis, in particular polymorphonuclear neutrophil (PMN) degranulation and vasculitis, bear resemblance to that seen in Wegener's granulomatosis, the latter being associated with the presence of anti-neutrophil cytoplasmic antibodies (ANCA). We therefore tested sera from patie...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Pudifin DJ,Duursma J,Gathiram V,Jackson TF

    更新日期:1994-07-01 00:00:00

  • Efficacy of HIV-specific and 'antibody-independent' mechanisms for complement activation by HIV-infected cells.

    abstract::Previous studies in this laboratory have shown that efficient activation of complement (C) on HIV isolates and HIV-infected cells requires the binding of specific anti-HIV antibodies, while other investigators have observed 'antibody-independent' C activation. In an attempt to clarify these disparate findings, we inve...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Saarloos MN,Lint TF,Spear GT

    更新日期:1995-02-01 00:00:00

  • Detection and characterization of liver membrane autoantibodies in chronic active hepatitis by a solid-phase radioimmunoassay.

    abstract::A solid-phase radioimmunoassay was developed to detect antibodies to liver membrane antigens in sera of patients with HBsAg-negative and -positive liver diseases and primary non-hepatic autoimmune diseases. Ten of fourteen patients with HBsAg-negative CAH had autoantibodies detected by RIA; negative results were obtai...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Manns M,Meyer zum Büschenfelde KH,Hütteroth TH,Hess G

    更新日期:1980-11-01 00:00:00

  • Patient-centred screening for primary immunodeficiency, a multi-stage diagnostic protocol designed for non-immunologists: 2011 update.

    abstract::Members of the European Society for Immunodeficiencies (ESID) and other colleagues have updated the multi-stage expert-opinion-based diagnostic protocol for non-immunologists incorporating newly defined primary immunodeficiency diseases (PIDs). The protocol presented here aims to increase the awareness of PIDs among d...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: de Vries E,European Society for Immunodeficiencies (ESID) members.

    更新日期:2012-01-01 00:00:00

  • Special anniversary review: twenty-five years of human immunodeficiency virus research: successes and challenges.

    abstract::During 25 years of research since HIV-1 was first identified in Paris, there have been great advances in our understanding of the virus and of the immune system. Practical advances include the early development of diagnostic tests of infection that made blood donation safe, and since 1996, combination anti-retroviral ...

    journal_title:Clinical and experimental immunology

    pub_type: 历史文章,杂志文章


    authors: Weiss RA

    更新日期:2008-05-01 00:00:00

  • In vitro effect of immune serum and bovine granulocytes on juvenile Fasciola hepatica.

    abstract::Cattle, infected with Fasciola hepatica metacercariae, produce antibodies against the outer glycocalyx of freshly excysted juvenile F. hepatica. Using 51Cr-release and viability assays such antibodies in the presence or absence of bovine complement did not cause discernible damage to the parasite. The presence of exce...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Duffus WP,Franks D

    更新日期:1980-09-01 00:00:00

  • Immunoglobulin (Ig)M antibodies against oxidized cardiolipin but not native cardiolipin are novel biomarkers in haemodialysis patients, associated negatively with mortality.

    abstract::The risk of premature death is high in haemodialysis (HD) patients. Antibodies against cardiolipin (anti-CL) are thrombogenic in diseases such as systemic lupus erythematosus (SLE). CL is easily oxidized (Ox) and plays a role in apoptosis. In this work we studied immunoglobulin (Ig)M anti-CL and anti-OxCL in HD-patien...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Frostegård AG,Hua X,Su J,Carrero JJ,Heimbürger O,Bárány P,Stenvinkel P,Frostegård J

    更新日期:2013-12-01 00:00:00

  • Incidence of selective IgG2 deficiency in patients with vasculitis.

    abstract::IgG2 deficiency has been recently associated with the appearance of vasculitis. None of the 42 patients with IgG2 deficiency whom we studied has a history of vasculitis. We have also studied 56 patients with vasculitis, and found four of them with IgG2 levels below the normal range for their age. As a group, the vascu...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Jiménez A,López-Trascasa M,Fontán G

    更新日期:1989-11-01 00:00:00

  • T cell regulation of the chronic peritoneal neutrophilia during mycobacterial infections.

    abstract::Intraperitoneal infection of mice with mycobacteria induces the persistent mobilization of neutrophils to the infected peritoneal cavities. The recruitment of the neutrophils was mediated by the immune system since it was enhanced by immunization and reduced in T cell-deficient nude and SCID mice. Anti-mitotic treatme...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Appelberg R

    更新日期:1992-07-01 00:00:00

  • Cytokine responses to the native and recombinant forms of the major surface glycoprotein of Pneumocystis carinii.

    abstract::Pneumocystis carinii is a major opportunistic pathogen and leading cause of morbidity in patients with AIDS. The major surface glycoprotein (MSG) of P. carinii, represented by a family of related proteins encoded by unique genes, is highly immunogenic and contains T cell-protective epitopes. We undertook the present s...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Theus SA,Smulian AG,Sullivan DW,Walzer PD

    更新日期:1997-08-01 00:00:00

  • GM-CSF and IL-12 production by malignant plasma cells promotes cell-mediated immune responses against monoclonal Ig determinants in a light chain myeloma model.

    abstract::Immune responses towards malignant plasma cells have clearly been demonstrated in the course of monoclonal B cell dyscrasias and shown to be mostly specific for idiotypic determinants of the monoclonal immunoglobulin (Ig). These responses are specifically efficient against lymphoma cells expressing a membrane form of ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Galea HR,Cogné M

    更新日期:2002-08-01 00:00:00

  • Immunological sequelae of intrauterine infection.

    abstract::Compared to normal children, pre-natally infected infants had significantly elevated IgM concentrations throughout the first year of life and elevated IgA levels for the first 6 months. In contrast, IgG levels dropped significantly below normal at 3 months post-partum. Infants born without overt disease but with eleva...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: McMurray DN,Rey H

    更新日期:1981-05-01 00:00:00