Bacterial lipopolysaccharide mediates the loss of CD4 from the surface of purified peripheral blood monocytes.


:In this study we have looked at the effect of lipopolysaccharide (LPS) on the surface antigen expression of cultured monocytes. Monocytes were purified from peripheral blood mononuclear cells (PBMC) and cultured in the presence or absence of LPS. The cultured cells were then stained with anti-MO3, anti-IL-2R and anti-CD4 MoAbs. We have shown that freshly isolated monocytes are IL-2R- and MO3-negative and express CD4 in low density. After overnight culture, without LPS, the expression of these surface markers remained relatively unchanged. However, in the presence of LPS (1 microgram/ml) CD4 expression was reduced to undetectable levels while the expression of IL-2R and MO3 was induced to maximal density. This effect of LPS on monocyte surface antigen expression was demonstrated with LPS preparations from Escherichia coli, Salmonella typhi and Vibrio cholerae. Surface antigen expression after 7 days culture in medium supplemented with non-heat-inactivated serum was essentially as seen after overnight culture, with the exception that LPS-induced IL-2R expression was transient. The ability to prepare monocytes that maintained surface CD4 expression after overnight culture was donor dependent.


Clin Exp Immunol


Neate EV,Greenhalgh AM,McPhee DA,Crowe SM




Has Abstract


1992-12-01 00:00:00












  • Blocking lymphotoxin-beta receptor activation diminishes inflammation via reduced mucosal addressin cell adhesion molecule-1 (MAdCAM-1) expression and leucocyte margination in chronic DSS-induced colitis.

    abstract::The lymphotoxin-beta receptor (LTbetaR) pathway is critical for maintenance of organized lymphoid structures and is involved in the development of colitis. To investigate the mechanisms by which LTbetaR activation contributes to the pathology of chronic inflammation we used a soluble LTbetaR-Ig fusion protein as a com...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Stopfer P,Obermeier F,Dunger N,Falk W,Farkas S,Janotta M,Möller A,Männel DN,Hehlgans T

    更新日期:2004-04-01 00:00:00

  • Interleukin-17 and systemic lupus erythematosus: current concepts.

    abstract::The emerging role of interleukin (IL)-17 as a hallmark proinflammatory cytokine of the adaptive immune system, produced primarily by a new T helper cell subset termed 'Th17', has received considerable attention. Differentiation of Th17 cells is driven by the simultaneous presence of transforming growth factor-beta and...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Nalbandian A,Crispín JC,Tsokos GC

    更新日期:2009-08-01 00:00:00

  • Is interferon-gamma the right marker for bacille Calmette-Guérin-induced immune protection? The missing link in our understanding of tuberculosis immunology.

    abstract::Bacille Calmette-Guérin (BCG), developed a century ago, is the only licensed tuberculosis (TB) vaccine in use to date. The protective efficacy of BCG against TB varies with no apparent protection in some population, and mechanisms of its immune protection is poorly known, and yet BCG is the most widely used vaccine, w...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Abebe F

    更新日期:2012-09-01 00:00:00

  • Autoantibodies to 200 kD polypeptide(s) of the nuclear envelope: a new serologic marker of primary biliary cirrhosis.

    abstract::Fifty sera which gave ring-like nuclear staining in immunofluorescence on rat liver tissue sections were characterized. Using immunoprecipitation and Western blotting we showed that 10 of the sera contained antibodies to 200 kD polypeptide(s) of nuclear envelope. Clinical and biological data were available for nine of...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Lassoued K,Guilly MN,Andre C,Paintrand M,Dhumeaux D,Danon F,Brouet JC,Courvalin JC

    更新日期:1988-11-01 00:00:00

  • Cytokine responses in acute and persistent human parvovirus B19 infection.

    abstract::The aim of this study was to characterize the proinflammatory and T helper (Th)1/Th2 cytokine responses during acute parvovirus B19 (B19) infection and determine whether an imbalance of the Th1/Th2 cytokine pattern is related to persistent B19 infection. Cytokines were quantified by multiplex beads immunoassay in seru...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Isa A,Lundqvist A,Lindblom A,Tolfvenstam T,Broliden K

    更新日期:2007-03-01 00:00:00

  • Expression and functional role of 1F7 (CD26) antigen on peripheral blood and synovial fluid T cells in rheumatoid arthritis patients.

    abstract::The expression and the functional role of the CD26 (1F7) T cell surface molecule, an ectoenzyme which seems to represent a functional collagen receptor of T lymphocytes and to have a role in T cell activation, were analysed in both peripheral blood (PB) and synovial fluid (SF) T cell samples from patients with active ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Muscat C,Bertotto A,Agea E,Bistoni O,Ercolani R,Tognellini R,Spinozzi F,Cesarotti M,Gerli R

    更新日期:1994-11-01 00:00:00

  • The interaction of anti-glomerular basement membrane antibody deposition with immune elimination of bovine serum albumin in the rabbit.

    abstract::We studied the interaction of two different forms of immune glomerular damage occurring simultaneously: anti-glomerular basement membrane (GBM) antibody fixation and immune elimination of bovine serum albumin (BSA). 125I-radiolabelled BSA anti-BSA immune complexes, formed in response to a single small intravenous dose...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Trevillian P,Cameron JS

    更新日期:1979-03-01 00:00:00

  • Prednisone increases apoptosis in in vitro activated human peripheral blood T lymphocytes.

    abstract::Glucocorticoid hormones (GCH) regulate, through the apoptotic process, the negative selection of immature T cells in the thymus. Because apoptosis seems to occur also in the maintenance of peripheral tolerance, we have investigated whether GCH may induce apoptosis in human mature lymphocytes. Peripheral blood lymphocy...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Lanza L,Scudeletti M,Puppo F,Bosco O,Peirano L,Filaci G,Fecarotta E,Vidali G,Indiveri F

    更新日期:1996-03-01 00:00:00

  • Epitope shift of proteinase-3 anti-neutrophil cytoplasmic antibodies in patients with small vessel vasculitis.

    abstract::Anti-neutrophil cytoplasmic antibodies against proteinase 3 (PR3-ANCA) are used as diagnostic tools for patients with small vessel vasculitis (AASV). We have produced chimeric mouse/human PR3 molecules and investigate changes in reactivity over time and the possible relationship between epitope specificity and clinica...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,多中心研究


    authors: Selga D,Segelmark M,Gunnarsson L,Hellmark T

    更新日期:2010-06-01 00:00:00

  • Human peripheral blood T lymphocyte subpopulations isolated on the basis of their affinity for sheep red blood cells differ in angiogenesis-inducing capability.

    abstract::Human peripheral blood lymphocytes injected intradermally into X-ray immunosuppressed mice were tested for angiogenesis-inducing capacity. Both T and B lymphocytes evoked angiogenesis of the same intensity. The total T cell population was fractionated into three subpopulations on the basis of their different affinitie...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kamiński MJ,Nowacyk M,Skopińska-Rozewska E,Kamińska G,Bem W

    更新日期:1981-11-01 00:00:00

  • Mice with early onset of death (EOD) due to lupus glomerulonephritis.

    abstract::Both MRL-lpr/lpr (lpr) and BXSB mice fall victim to autoimmune disease as a function of age. To combine their properties, brother-sister mating of (female lpr x male BXSB)F1 mice was done. Mice for mating were selected according to indicators of early onset of glomerulonephritis and subsequent early death (i.e., EOD)....

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Honda S,Nemoto K,Mae T,Kinjoh K,Kyogoku M,Kawamura H,Miyazawa S,Weerashinghe A,Watanabe H,Narita J,Koya T,Arakawa M,Abo T

    更新日期:1999-04-01 00:00:00

  • Quantitation of HLA-DR expression by cells involved in the skin lesions of tuberculoid and lepromatous leprosy.

    abstract::A method is described which can be used to quantitate class II MHC antigens (HLA-DR) expressed by cells within tissue sections. A mouse anti-human HLA-DR monoclonal antibody is directly conjugated to the fungal enzyme glucose oxidase. The enzyme, in the presence of its substrate can be used to reduce tetrazolium salts...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Collings LA,Tidman N,Poulter LW

    更新日期:1985-07-01 00:00:00

  • Bone marrow and peripheral blood natural killer cell activity in lymphomas. Its response to IL-2.

    abstract::Natural killer (NK) cytotoxic activity was simultaneously investigated in bone marrow mononuclear cells (BMMC) and peripheral blood lymphocytes (PBL) from nine Hodgkin's disease (HD) and 15 non-Hodgkin lymphoma (NHL) untreated patients. Twenty-five PBL samples and seven bone marrow specimens from healthy individuals w...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Caldera LH,Leon-Ponte M,Acquatella G,Bianco NE,Blanca I

    更新日期:1992-04-01 00:00:00

  • Quantitative intracellular cytokine measurement: age-related changes in proinflammatory cytokine production.

    abstract::The proinflammatory cytokines play a central role in mediating cellular and physiological responses, and levels may reflect immune system effectiveness. In this study, the effect of ageing on the inflammatory response was examined using a novel method to detect production of the proinflammatory cytokines, i.e. tumour ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: O'Mahony L,Holland J,Jackson J,Feighery C,Hennessy TP,Mealy K

    更新日期:1998-08-01 00:00:00

  • In vitro cellular immunity in Chagas' disease.

    abstract::In vitro delayed hypersensitivity was studied in patients with either the chronic or the indeterminate phase of Chagas' disease. Normal healthy individuals were used as controls. The leucocyte migration inhibition test was applied using an antigen an extract of culture forms of T. cruzi or an extract of normal rat hea...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Toledo Barros MA,Amato Neto V,Mendes E,Mota I

    更新日期:1979-11-01 00:00:00

  • Spontaneous CD30 mRNA expression in peripheral blood mononuclear cells from atopic patients with high IgE serum levels.

    abstract::CD30 is a surface molecule which can be expressed by normal B and T lymphocytes. Our study focused on the CD30 expression and release compared with IL-4 expression as well as CD23-alpha/beta in peripheral blood mononuclear cells (PBMC) from atopic subjects and controls. Data showed a lack of CD30 mRNA expression in th...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Esnault S,Benbernou N,Lavaud F,Guenounou M

    更新日期:1996-10-01 00:00:00

  • Alteration in T lymphocyte subpopulations in inflammatory bowel disease.

    abstract::Peripheral blood mononuclear cells from thirty-one patients with inflammatory bowel disease (ulcerative colitis and Crohn's disease) were analysed for the proportions and absolute numbers of total T cells, and for the T cell subpopulations carrying Fc receptors for either IgM (Tmu cells) or IgG (T gamma cells). Twenty...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Victorino RM,Hodgson HJ

    更新日期:1980-07-01 00:00:00

  • Interleukin-6 blockade in ocular inflammatory diseases.

    abstract::Interleukin-6 (IL-6) is a key cytokine featuring redundancy and pleiotropic activity. It plays a central role in host defence against environmental stress such as infection and injury. Dysregulated, persistent interleukin (IL)-6 production has been implicated in the development of various autoimmune, chronic inflammat...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Mesquida M,Leszczynska A,Llorenç V,Adán A

    更新日期:2014-06-01 00:00:00

  • IgE rheumatoid factors: quantification in synovial fluid and ability to induce synovial mast cell histamine release.

    abstract::IgE rheumatoid factor activity was found to be significantly elevated (P less than 0.01) using an ELISA assay when paired synovial fluid and sera from 13 patients with active RA were compared to ten control samples. Synovial fluid IgE RF activity was higher than predicted by diffusion alone in 7/11 (64%) of the RA syn...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Gruber B,Ballan D,Gorevic PD

    更新日期:1988-02-01 00:00:00

  • Mammalian Toll-like receptors: to immunity and beyond.

    abstract::Toll-like receptors (TLRs) constitute an archetypal pattern recognition system. Their sophisticated biology underpins the ability of innate immunity to discriminate between highly diverse microbial pathogens and self. However, the remarkable progress made in describing this biology has also revealed new immunological ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Hopkins PA,Sriskandan S

    更新日期:2005-06-01 00:00:00

  • Prolonged disturbances of in vitro cytokine production in patients with severe acute respiratory syndrome (SARS) treated with ribavirin and steroids.

    abstract::Severe acute respiratory syndrome (SARS) is a new disease which has spread rapidly and widely. We wished to know whether evaluation of in vitro cytokine production could contribute to improved understanding of disease pathogenesis and to better patient management. Numbers of unstimulated and mitogen-stimulated cytokin...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Jones BM,Ma ES,Peiris JS,Wong PC,Ho JC,Lam B,Lai KN,Tsang KW

    更新日期:2004-03-01 00:00:00

  • In vitro exposure of human fibroblasts to local anaesthetics impairs cell growth.

    abstract::Lidocaine, bupivacaine or ropivacaine are used routinely to manage perioperative pain. Sparse data exist evaluating the effects of local anaesthetics (LA) on fibroblasts, which are involved actively in wound healing. Therefore, we investigated the effects of the three LA to assess the survival, viability and prolifera...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Fedder C,Beck-Schimmer B,Aguirre J,Hasler M,Roth-Z'graggen B,Urner M,Kalberer S,Schlicker A,Votta-Velis G,Bonvini JM,Graetz K,Borgeat A

    更新日期:2010-11-01 00:00:00

  • The CD8+ Leu-7+ subset of T cells in Crohn's disease: distinction between cytotoxic and covert suppressor functions.

    abstract::An expanded T cell subpopulation (CD8+ Leu-7+) has previously been reported in the peripheral blood of patients with Crohn's disease. This subpopulation of T cells was associated with a 'covert suppressor' function, particularly in patients with mild/early Crohn's disease, suppressing immunoglobulin production in vitr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Deem RL,Targan SR,Niederlehner A,Shanahan F

    更新日期:1990-06-01 00:00:00

  • Prolonged circulation of immune complexes due to various altered immune functions contributes to nephritis in MRL/lpr mice.

    abstract::To gain some insight into the pathogenesis of proliferative lupus nephritis in MRL/lpr mice we investigated the kinetics of removal of immune complexes from the circulation, the carrier state of blood cells, the uptake of complexes by the mononuclear phagocyte system, and the localization of complexes in kidneys. In n...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Granholm NA,Cavallo T

    更新日期:1990-11-01 00:00:00

  • Augmented production of chemokines (monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-1alpha (MIP-1alpha) and MIP-1beta) in patients with systemic sclerosis: MCP-1 and MIP-1alpha may be involved in the development of pulmonary fibros

    abstract::To determine the role of chemokines in the pathogenesis of systemic sclerosis (SSc), we examined serum levels, spontaneous production by peripheral blood mononuclear cells (PBMC), and histological distribution in the affected skin, of MCP-1, MIP-1alpha and MIP-1beta in SSc patients. Serum levels of these chemokines we...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Hasegawa M,Sato S,Takehara K

    更新日期:1999-07-01 00:00:00

  • Epstein-Barr serology in immunodeficiencies: an attempt to correlate with immune abnormalities in Wiskott-Aldrich and Chediak-Higashi syndromes and ataxia telangiectasia.

    abstract::Epstein-Barr (EB) virus serology was correlated with the results of immunological investigations of three inherited immunodeficiency diseases, in an attempt to understand the immune mechanisms controlling EB virus infection. In nine patients with Wiskott-Aldrich syndrome (WAS), the constant lack of anti-EB virus assoc...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Vilmer E,Lenoir GM,Virelizier JL,Griscelli C

    更新日期:1984-02-01 00:00:00

  • Anti-CD2 (OX34) MoAb treatment of adjuvant arthritic rats: attenuation of established arthritis, selective depletion of CD4+ T cells, and CD2 down-modulation.

    abstract::Anti-CD2 MoAbs have previously been shown to induce tolerance and to block B cell differentiation, T cell and monocyte activation. Since these immune functions are important in joint inflammation, we asked whether administration of the anti-CD2 MoAb OX34 has a beneficial effect on established rat adjuvant arthritis, a...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Hoffmann JC,Herklotz C,Zeidler H,Bayer B,Westermann J

    更新日期:1997-10-01 00:00:00

  • The avidity of allospecific cytotoxic T lymphocytes (CTL) determines their cytokine production profile.

    abstract::Donor-specific CTL present within the cardiac allograft during a rejection episode are distinct from those that populate the cardiac allograft in the absence of rejection. Whereas the former generally have a high avidity for donor cells, the latter mainly have a low avidity for donor cells. This observation made us re...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Van Emmerik NE,Daane CR,Knoop CJ,Hesse C,Vaessen LM,Balk AH,Mochtar B,Claas FH,Weimar W

    更新日期:1997-12-01 00:00:00

  • The role of stromal cells in the persistence of chronic inflammation.

    abstract::Inflammation is an unstable state; it either resolves or persists. Inflammatory reactions often have a propensity for specific anatomical sites. Why inflammation persists with specific tissue tropism remains obscure. Increasing evidence suggests that stromal cells which define tissue architecture are the key cells inv...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Naylor AJ,Filer A,Buckley CD

    更新日期:2013-01-01 00:00:00

  • Endogenous interleukin-22 protects against inflammatory bowel disease but not autoimmune cholangitis in dominant negative form of transforming growth factor beta receptor type II mice.

    abstract::During chronic inflammation, interleukin (IL)-22 expression is up-regulated in both CD4 and CD8 T cells, exerting a protective role in infections. However, in autoimmunity, IL-22 appears to have either a protective or a pathogenic role in a variety of murine models of autoimmunity and, by extrapolation, in humans. It ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Yang GX,Sun Y,Tsuneyama K,Zhang W,Leung PS,He XS,Ansari AA,Bowlus C,Ridgway WM,Gershwin ME

    更新日期:2016-08-01 00:00:00