Abstract:
:We have evaluated a new immunoradiometric assay (IRMA) for calcitonin (CT) (Medgenix, Belgium). The detection limit of immunoreactive CT (IR-CT) was 2.8 pg/ml, and plasma levels of IR-CT of 134 healthy adults (dynamic range, 2.8-1000 pg/ml) were < 19 pg/ml (median 4.1 pg/ml). Both basal and pentagastrin-stimulated IR-CT levels were < 30 pg/ml and were well separated from the levels in patients with medullary thyroid carcinoma (MTC) (46-29,000 pg/ml, n = 38). In preoperative plasma samples in five patients with C-cell hyperplasia, CT IRMA confirmed the diagnosis, although in two patients RIA failed to detect a significant increase of IR-CT. By modifying the IRMA, we could identify residual tumor tissues perioperatively in patients undergoing thyroidectomy for MTC. RIA of gel-permeation chromatography showed four IR-CT peaks, but IRMA had only three peaks, suggesting that IRMA is more specific. CT-IRMA is more specific, sensitive, robust, rapid, and reliable than RIA. In addition, neat plasma can be used directly in a single step in CT-IRMA.
journal_name
Peptidesjournal_title
Peptidesauthors
Wimalawansa SJ,Bailey Fdoi
10.1016/0196-9781(94)00177-4subject
Has Abstractpub_date
1995-01-01 00:00:00pages
307-12issue
2eissn
0196-9781issn
1873-5169pii
0196-9781(94)00177-4journal_volume
16pub_type
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