Abstract:
:An antifungal peptide with a molecular mass of 9 kDa was isolated from fresh fruiting bodies of the mushroom Agrocybe cylindracea. The isolation procedure comprised ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatography (FPLC) on Mono S, and FPLC-gel filtration on a Superdex 75 column. The antifungal peptide, designated as agrocybin, was unadsorbed on DEAE-cellulose, and adsorbed on Affi-gel blue gel and Mono S. Agrocybin exerted antifungal activity against several fungal species but lacked inhibitory activity against bacteria when tested up to 300 microM. The activity of HIV-1 reverse transcriptase was attenuated in the presence of agrocybin. It exhibited weaker mitogenic activity than Con A on isolated murine splenocytes, but was devoid of antiproliferative activity on Hep G2 (hepatoma) cells when tested at 110 microM.
journal_name
Peptidesjournal_title
Peptidesauthors
Ngai PH,Zhao Z,Ng TBdoi
10.1016/j.peptides.2004.09.011subject
Has Abstractpub_date
2005-02-01 00:00:00pages
191-6issue
2eissn
0196-9781issn
1873-5169pii
S0196-9781(04)00422-Xjournal_volume
26pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::Angiotensin II (ANG II) receptors were present in the thymus of newborn rats (179 +/- 34 fmol/mg protein). In newborns, binding was predominantly localized in the trabecula, and was selectively displaced by the AT2 ligand CGP 42112A (83-85%) and to a lower extent by the AT1 antagonist losartan (15-17%), indicating a m...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)90036-1
更新日期:1994-01-01 00:00:00
abstract::C-type natriuretic peptide (CNP) was recently found in myocardium at the mRNA and protein levels, but it is not known whether cardiomyocytes are able to produce CNP. The aim of this study was to determine the expression of CNP and its specific receptor NPR-B in cardiac cells, both in vitro and ex vivo. CNP, brain natr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.06.014
更新日期:2011-08-01 00:00:00
abstract::VIP stimulates adenylate cyclase activity of male and female rat anterior pituitaries and human prolactinomas, while dopamine inhibits the enzyme activity of female rat pituitaries and prolactinomas. A dopamine inhibited cyclase can be detected also in male rats provided the enzyme activity is increased by VIP. The an...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(84)90172-4
更新日期:1984-11-01 00:00:00
abstract::This study sought to determine the serum levels of chemerin in gastric cancer patients and healthy subjects and to investigate the biological effect of chemerin on gastric cancer cells. Serum chemerin level of 36 gastric cancer patients and 40 healthy subjects was measured by enzyme-linked immunosorbent assay. AGS and...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2013.10.009
更新日期:2014-01-01 00:00:00
abstract::Dissociated muscle cells prepared from hearts of the pulmonate snail Helix aspersa were used to study signal transduction induced by molluscan cardioactive peptides. The effects of SCPb on the cAMP levels of whole hearts and the cell preparation were compared. The cells responded to SCPb with a dose-dependent increase...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(97)00196-4
更新日期:1997-01-01 00:00:00
abstract::Kyotorphin is a unique biologically active neuropeptide (l-tyrosine-l-arginine), which is reported to have opioid-like analgesic actions through a release of Met-enkephalin from the brain slices. N-methyl-l-tyrosine-l-arginine (NMYR), an enzymatically stable mimetic of kyotorphin, successfully caused potent analgesic ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2018.06.010
更新日期:2018-09-01 00:00:00
abstract::Various growth factors and their receptors are present in the nervous system. This review focuses on the presence of epidermal growth factor (EGF) and its receptors in the central nervous system (CNS). Evidence indicates that EGF in the CNS is the result of local synthesis, by intrinsic and blood-derived macrophages, ...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/0196-9781(91)90115-6
更新日期:1991-05-01 00:00:00
abstract::We examined the expressions of the prepro-orexin gene in the lateral hypothalamic area (LHA), the genes of the neuropeptide Y (NPY) and proopiomelanocortin (POMC) in the arcuate nucleus (ARC), the orexin type 1 receptor (OX1R) gene in the ventromedial hypothalamic nucleus (VMH) and the orexin type 2 receptor (OX2R) ge...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(02)00111-0
更新日期:2002-09-01 00:00:00
abstract::We compared the effects of three micro-(DAMGO, DALDA, TNPO) and three delta-(DPDPE, DELT, SNC-80) opioid agonists on arterial blood gas after IV administration in awake sheep. None of the mu agonists altered pO2, pCO2 or pH. All three mu agonists decreased pO2 increased pCO2 and decreased pO2, and this effect was not ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(98)00141-7
更新日期:1999-01-01 00:00:00
abstract::This paper reviews aspects of NPY research that were emerging in 1985, shortly after the isolation and characterization of the peptide. NPY had become known for its widespread distribution especially in the central and peripheral nervous systems, but also in the gastro-intestinal and respiratory tracts and in fibers i...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2004.02.013
更新日期:2004-03-01 00:00:00
abstract::Two peptides, with a molecular mass of about 11 kDa and an N-terminal sequence abundant in arginine and glutamine residues, were isolated from wax gourd seeds. The isolation protocol included affinity chromatography on Affi-gel blue gel, ion-exchange chromatography on Mono-S and gel filtration on Superdex 75. The pept...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(02)00271-1
更新日期:2003-01-01 00:00:00
abstract::This paper reviews aspects of our research, focusing on the role of the melanocortin system in the central regulation of feeding and energy balance, which was begun in 1997. It describes data from successive physiological studies, concerning the identity of the appetite-regulating melanocortin receptor, melanocortin-4...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2005.01.030
更新日期:2006-02-01 00:00:00
abstract::We previously demonstrated that intracerebroventricular (i.c.v.) administration of the substance P (SP) aminoterminal fragment SP(1-7) attenuates the expression of morphine withdrawal in the male rat. In this study we have used a synthetic analogue of this peptide, i.e. the SP(1-7) amide showing higher binding potency...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.08.009
更新日期:2009-12-01 00:00:00
abstract::The presence of a putative GRP receptor on rat pancreatic particulate membranes was demonstrated by covalent cross-linking to 125I-gastrin releasing peptide (GRP), which revealed a radioactive band with Mr = 80-90 kDa on reduced SDS-PAGE. Fresh rat pancreatic membranes contained a GRP receptor which was solubilized wi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90001-6
更新日期:1991-03-01 00:00:00
abstract::Growing evidence suggests that the neuropeptide Y (NPY) system plays an important role in the immune system. Yet, little is known about the expression of NPY and receptors in the immune system. Moreover, original contradicting results have confused the picture and hampered a clear understanding of its role in the immu...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2006.09.030
更新日期:2007-02-01 00:00:00
abstract::Five native pyrokinin-like peptides (Neb-PK-1, Neb-PK-2, Neb-PVK-1, [L9]Neb-PVK-2, [I9]Neb-PVK-2) identified in the neuropeptidome of the flesh fly Neobellieria bullata were compared for their quantitative and/or qualitative effects on puparium formation (pupariation). In a standard pupariation bioassay, both Neb-PVK-...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.08.023
更新日期:2006-03-01 00:00:00
abstract::Capsaicin-sensitive sensory nerves participate in the regulation of cardiovascular functions both in the normal state and the pathophysiology of hypertension through the actions of potent vasodilator neuropeptides, including calcitonin gene-related peptide (CGRP). CGRP, a very potent vasodilator, is the predominant ne...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2005.02.002
更新日期:2005-09-01 00:00:00
abstract::Biological and immunological relationships between molt-inhibiting hormone (MIH) activity in eyestalk ganglia extracts of the crab, Cancer antennarius Stimpson, and peptides of the vasopressin-oxytocin family were assessed. Lysine vasopressin (LVP), arginine vasopressin (AVP), vasotocin (VT), and oxytocin (OT) mimicke...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90166-4
更新日期:1985-07-01 00:00:00
abstract::By using specific antibodies and radioimmunological and immunohistochemical methods, we here show that neuropeptide FF (NPFF) occurs in cervical and lumbar dorsal root ganglia cells. Levels in the ganglia were low because they were detectable only after colchicine treatment or after unilateral dorsal rhizotomy. Simila...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(99)00039-x
更新日期:1999-01-01 00:00:00
abstract::Numerous works associate the MCH peptide, and the hypothalamic neurons that produce it, to the feeding behavior and energy homeostasis. It is commonly admitted that MCH is an orexigenic peptide, and MCH neurons could be under the control of arcuate NPY and POMC neurons. However, the literature data is not always conco...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2009.07.008
更新日期:2009-11-01 00:00:00
abstract::Intracerebroventricular (ICV) bombesin increases grooming and decreases food intake in rats. We examined tolerance to these effects by administering a daily injection of either saline or 25 ng bombesin to rats for 8 days via lateral ventricular cannulas. Food intake and grooming were monitored. After 8 days bombesin n...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(92)90031-w
更新日期:1992-11-01 00:00:00
abstract::Two molecular forms of gonadotropin-releasing hormone (GnRH) were identified in the extracts of various brain areas, spinal cord and pituitary in female and male goldfish and had chromatographic and immunological properties similar to [His5, Trp7, Tyr8]-GnRH (cGnRH-II) and [Trp7,Leu8]-GnRH (sGnRH). Radioimmunoassay us...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(88)90174-x
更新日期:1988-05-01 00:00:00
abstract::We examined the effects of tumor necrosis factor (TNF)-alpha on the expression and functionality of adrenomedullin (AM) receptors in cultured human coronary artery smooth muscle cells. Analysis of real-time quantitative polymerase chain reactions showed that these cells abundantly express two AM receptors comprised of...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2004.04.010
更新日期:2004-07-01 00:00:00
abstract::Endokinins are novel tachykinins encoded on the human TAC4 and consist of Endokinin A (EKA), B (EKB), C (EKC) and D (EKD). To date, the function of Endokinins in pain processing was not fully understood. Therefore the aim of this study was to investigate the effects of Endokinin A/B (EKA/B, the common C-terminal decap...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.10.013
更新日期:2010-01-01 00:00:00
abstract::The neuropharmacological basis for d-amphetamine induced paradoxical behavioral thermoregulation remains unclear. This study examined thermoregulatory behavior of rats in a runway device that housed a heat source at one end and in which locomotion along the length of the runway could be observed. Sprague Dawley rats w...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90038-5
更新日期:1985-03-01 00:00:00
abstract::Many animal studies show an enhancing effect of vasopressin (VP) on memory, but not all human studies could confirm this finding. This study examined the influence of post-learning administration of VP (40 IU, intranasally) on the consolidation of declarative memories in healthy humans during different intervals of sl...
journal_title:Peptides
pub_type: 临床试验,杂志文章
doi:10.1016/s0196-9781(01)00625-8
更新日期:2002-03-01 00:00:00
abstract::CGRP has potent cardiovascular effects but its role in heart failure is unclear. Effects of CGRP on calcium concentrations in fresh adult rat cardiomyocytes, cultured adult cardiomyocytes and neonatal cardiomyocytes were determined by real time fluorescence spectrophotometry. Treatment of cultured adult cardiomyocytes...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2004.10.020
更新日期:2005-03-01 00:00:00
abstract::Nervous, endocrine, and immune systems share a large number of regulatory molecules including hormones and neuropeptides. Vasoactive intestinal peptide (VIP) plays an important role in a variety of immunological functions. In the present report, we sorted purified thymocytes of the four major thymic subsets defined by...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(96)00070-8
更新日期:1996-01-01 00:00:00
abstract::Previous studies have shown that different calcitonins interact with planar lipid membranes to form ion channels. In this study, glycosylation of eel calcitonin (eCt) at different positions (Ct3-GlcNAc, Ct14-GlcNAc, Ct20-GlcNAc, Ct26-GlcNAc) is shown to preserve molecular structure and slightly change the energy of in...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.09.011
更新日期:2006-04-01 00:00:00
abstract::Immunohistochemical studies were conducted on sections of cat hypothalamus in order to determine the distribution of neurons containing alpha-melanocyte stimulating hormone and beta-endorphin immunoreactivity. A large number of neurons in the arcuate nucleus were stained after incubation of sections with antisera to e...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(82)90167-x
更新日期:1982-07-01 00:00:00