Abstract：
:The presence of a putative GRP receptor on rat pancreatic particulate membranes was demonstrated by covalent cross-linking to 125I-gastrin releasing peptide (GRP), which revealed a radioactive band with Mr = 80-90 kDa on reduced SDS-PAGE. Fresh rat pancreatic membranes contained a GRP receptor which was solubilized with Triton X-100 as assessed by its failure to sediment at 100,000 x g for one hour and its ability to pass through a 0.22 mu filter. When 125I-GRP binding was studied using Sephadex G50 gel filtration chromatography to separate bound from unbound ligand, substantial amounts of 125I-GRP binding were observed in rat crude solubilized pancreatic membranes, but essentially no specific binding was observed until the crude solubilized membranes were fractionated by ammonium sulfate precipitation. Specific 125I-GRP binding was 500, 700 and 1400 fmol/mg protein, respectively, in the 0-25%, 25-50% and 50-80% saturated ammonium sulfate fractions (125I-GRP concentration = 1 nM). Specific binding was temperature dependent, saturable and of high affinity, (KD = 2.3 nM). A unique 70 kDa band was visualized by silver staining of the SDS-PAGE of eluates of GRP(14-27) affinity gel compared with eluates of control affinity gels incubated with the 25-50% (NH4)2SO4 fraction. The lower Mr than that observed with covalent cross-linking may represent the binding subunit of a larger receptor protein. This ligand-affinity isolated protein is thus a good candidate for the GRP receptor, or the binding subunit of it, from normal rat pancreas.
journal_name
Peptidesjournal_title
Peptidesauthors
Kane MA,Kelley K,Ross SE,Portanova LBdoi
10.1016/0196-9781(91)90001-6subject
Has Abstractpub_date
1991-03-01 00:00:00pages
207-13issue
2eissn
0196-9781issn
1873-5169pii
0196-9781(91)90001-6journal_volume
12pub_type
杂志文章相关文献
PEPTIDES文献大全abstract：:We examined the effect of adjuvant arthritis on the content of immunoreactive calcitonin gene-related peptide (iCGRP) in the dorsal root ganglia at L4-L6 levels and the spinal cord at a lumbar level in rats. Arthritis was induced by inoculating adjuvant into both hind-paws twice at a 10 day interval. In the arthritic ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(89)90057-0
更新日期：1989-03-01 00:00:00
abstract：:Cholecystokinin octapeptides (CCK8s) have been purified from methanol extracts of two brains from each of two Australian marsupials, Tammar Wallaby and Eastern Quoll, containing 3 nmol and 2 nmol of the peptides, respectively. Immunoreactive CCK was concentrated on QMA SepPak cartridges and purified by two successive ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(88)90280-x
更新日期：1988-03-01 00:00:00
abstract：:The last decades have seen no significant progress in extending the survival of lung cancer patients and there is an urgent need to improve current therapies. The substance P (SP)/neurokinin-1 receptor (NK-1R) system plays an important role in the development of cancer: SP and NK-1R antagonists respectively induce cel...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2012.09.024
更新日期：2012-12-01 00:00:00
abstract：:The presence of human melanin-concentrating hormone (MCH) was studied in the human brain by radioimmunoassay and immunocytochemistry. Immunoreactive MCH concentrations in the human brain ranged from 0.07 to 19.7 pmol/g wet weight. High performance liquid chromatography of the hypothalamus showed a large immunoreactive...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(93)90158-d
更新日期：1993-05-01 00:00:00
abstract：:Cellular uptake of [125I] labelled DSIP at the luminal interface of the blood-brain barrier (BBB) was studied in the ipsilateral perfused in situ guinea pig forebrain. Regional unidirectional transfer constants (Kin) calculated from the multiple-time brain uptake analysis were 0.93, 1.33 and 1.66 microliter.min-1 g-1 ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(89)90026-0
更新日期：1989-03-01 00:00:00
abstract：:On attempts to identify toxins showing original profile of activity among K+ channels, we purified Kbot1, a scorpion toxin that blocks Kv1 and SK potassium channels. With 28 amino-acid residues, Kbot1 is the shortest toxin sequenced in Buthus occitanus scorpion. It is linked by three disulfide bridges and its primary ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2004.02.017
更新日期：2004-04-01 00:00:00
abstract：:A new, p -carborane containing analog of tyrosine, 3-[1-hydroxy-1,12-dicarba-closo-dodecaboran (12)-12-yl]alanine, was prepared from protected 3-[1-hydroxy-1,12-dicarba-closo-dodecaboran (12)-12-yl]propionic acid in five steps using Oppolzer's sultam methodology for asymmetric hydroxyamination as the key step. The tyr...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(01)00650-7
更新日期：2002-04-01 00:00:00
abstract：:Circulating urotensin II (UII) concentrations and the tissue expression of its cognate receptor (UT) are elevated in patients with cardiovascular disease (CVD). The functional significance of elevated plasma UII levels in CVD is unclear. Urotensin-related peptide (URP) is a paralog of UII in that it contains the six a...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2007.08.013
更新日期：2008-05-01 00:00:00
abstract：:Acute and chronic effects of gamma-butyrolactone-gamma-carbonyl-histidyl-prolinamide (DN-1417) were investigated on motor activity, dopamine (DA) metabolites and DA receptors in various brain regions of rats. The motor activity, as measured with Automex recorder, was enhanced after a single injection with DN-1417 (20 ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(85)90433-4
更新日期：1985-11-01 00:00:00
abstract：:This paper reviews aspects of our research, focusing on the role of the melanocortin system in the central regulation of feeding and energy balance, which was begun in 1997. It describes data from successive physiological studies, concerning the identity of the appetite-regulating melanocortin receptor, melanocortin-4...
journal_title：Peptides
pub_type： 杂志文章,评审
doi：10.1016/j.peptides.2005.01.030
更新日期：2006-02-01 00:00:00
abstract：:In the Nucleus Ambiguus (NA) mu-receptors increased mean arterial pressure (MAP) and heart rate (HR) and kappa-receptors decreased MAP without altering HR. Mu-receptors in the Dorsal Motor Nucleus of the Vagus (DMV) increased MAP without changing HR whereas kappa-receptors in the DMV decreased HR without changing MAP....
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(88)90225-2
更新日期：1988-01-01 00:00:00
abstract：:Neuropeptide Y (NPY) is one of the most potent stimulants of food intake in mammals, but very little is known about NPY actions in fish. The present study investigated the role of NPY in food intake in the rainbow trout (Oncorhynchus mykiss). Food intake was monitored at different times after intracerebroventricular a...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2005.09.014
更新日期：2006-04-01 00:00:00
abstract：:Endocrine tumor cells in culture and in vitro cleavage assays have shown that PC1 and PC2 are capable of processing pro-CCK into smaller, intermediate and final, bioactive forms. Similar studies have shown that PC5 has the ability to process a number of propeptides. Here, we use GT1-7 (mouse hypothalamic) and SK-N-MC ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(01)00451-x
更新日期：2001-08-01 00:00:00
abstract：:Self-administration of ethanol and food share many common features and Richter hypothesized that an increase in ethanol consumption would decrease feeding to balance the excess calories contained in the ethanol. Previously, we have shown that individual alcohol consumption correlates with neurotransmitter gene express...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2007.09.011
更新日期：2007-12-01 00:00:00
abstract：:We examined the mechanism through which leptin increases Na(+), K(+)-ATPase activity in the rat kidney. Leptin was infused under anaesthesia into the abdominal aorta proximally to the renal arteries and then Na(+), K(+)-ATPase activity was measured in the renal cortex and medulla. Leptin (1mug/kgmin) increased Na(+), ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2006.08.010
更新日期：2006-12-01 00:00:00
abstract：:Apamin is a neurotoxic octadecapeptide from bee venom, which has been shown to inhibit the non-adrenergic, non-cholinergic inhibitory innervation of the smooth muscle of the gut. Since vasoactive intestinal polypeptide (VIP) has been proposed as a possible inhibitory neurotransmitter, the effect of apamin on the recep...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(83)90102-x
更新日期：1983-03-01 00:00:00
abstract：:Plant sucking heteropteran bugs feed regularly on small amounts of K(+)-rich plant material, in contrast to their hematophagous relatives which imbibe large volumes of Na(+)-rich blood. It was anticipated that this would be reflected in the endocrine control of Malpighian tubule (MT) secretion. To explore this, neuroe...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2009.09.017
更新日期：2010-03-01 00:00:00
abstract：:Venoms from cone snails (genus Conus) can be seen as an untapped cocktail of biologically active compounds, being increasingly recognized as an emerging source of peptide-based therapeutics. Cone snails are considered to be specialized predators that have evolved the most sophisticated peptide chemistry and neuropharm...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2012.07.002
更新日期：2013-03-01 00:00:00
abstract：:Neuropeptide S (NPS), the endogenous ligand of NPS receptor (NPSR), regulates many biological functions, including arousal, anxiety, locomotion and food intake. NPSR mRNA is expressed in several regions of central autonomic network through which the brain controls visceromotor and other responses essential for surviva...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2009.03.012
更新日期：2009-07-01 00:00:00
abstract：:Dissociated neurointermediate lobe cells (75%) bound [biotinyl-Ser1]corticotropin releasing hormone (bio-CRH) and secreted adrenocorticotropin [ACTH(25-39)] in a reverse hemolytic plaque assay (RHPA). The bio-CRH was rapidly internalized (less than 1 min) in vesicles and scattered vacuoles. Plaque percentages increase...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(90)90188-b
更新日期：1990-07-01 00:00:00
abstract：:Insulin has been detected by ELISA in the vitreous of the normal and streptozotocin-diabetic rat at levels for both about 1% of those in serum. 131I-labeled insulin, administered to conscious rats via an indwelling cannula in the right atrium, was found to cross the blood-ocular barrier into the vitreous. Autoradiogra...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(92)90171-x
更新日期：1992-07-01 00:00:00
abstract：:The presence of alpha-hANP immunoreactive material in human heart and plasma was investigated with a specific and sensitive radioimmunoassay and immunohistochemical method. It was found that alpha-hANP immunoreactive staining of specific atrial granules was located around the nucleus of atrial cardiocytes. No immunore...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(86)90057-4
更新日期：1986-01-01 00:00:00
abstract：:The guinea pig ileum possesses NK-1 and NK-3 tachykinin receptors. As expected, [Pro9]SP and senktide, which are selective agonists of NK-1 and NK-3 receptors, respectively, were found to be highly potent in contracting the guinea pig ileum. Surprisingly, similar observations were made with septide, SP-O-CH3, [Apa9-10...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(92)90125-m
更新日期：1992-03-01 00:00:00
abstract：:Which of Y1-Y5 receptors (Rs) mediate NPY's angiogenic activity was studied using Y2R-null mice and R-specific antagonists. In Y2R-null mice, NPY-induced aortic sprouting and in vivo Matrigel capillary formation were decreased by 50%; Y1R-antagonist blocked the remaining response. NPY-induced sprouting was equally inh...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(02)00281-4
更新日期：2003-01-01 00:00:00
abstract：:This study aims to examine the antidepressant-like action of Ghrelin (Ghr), a hormone synthesized predominantly by gastrointestinal endocrine cells and released during periods of negative energy balance, in two behavioral models: tail suspension test (TST), a predictive model of antidepressant activity, and the olfact...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2012.03.031
更新日期：2012-06-01 00:00:00
abstract：:The biological activities of alpha-MSH des-acetyl MSH, gamma-MSH and LPH37-58 were compared using the Anolis rate method of bioassay. Dose-response data showed LPH37-58 to be equipotent with alpha-MSH, but des-acetyl MSH and gamma-MSH were found to be much less active. The effect of LPH37-58 was additive to that of al...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(82)90134-6
更新日期：1982-01-01 00:00:00
abstract：:Mate attraction in Aplysia involves the long-distance water-borne protein pheromones attractin, enticin, and temptin which are released during egg-laying. Other water-borne pheromones are predicted to act in concert with attractin, enticin, and temptin, but their identities were unknown. We recently identified a highl...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2004.10.024
更新日期：2005-03-01 00:00:00
abstract：:A pancreatic ductal carcinoma was established as a transplantable tumor line in an inbred strain of Syrian golden hamsters. Intracellular recordings of membrane potentials and input resistance were made from cultured cells obtained from the transplanted tumors using indwelling glass microelectrode. The mean value of t...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(84)90247-x
更新日期：1984-03-01 00:00:00
abstract：:The relaxant effect of a novel VIP analog, [Arg15,20,21Leu17]-VIP-Gly-Lys-Arg-NH2 was compared with that of the original VIP in the same guinea pig trachea precontracted by carbachol in vitro. The VIP analog caused significantly and concentration-dependent relaxation similarly to the original VIP. In contrast to the o...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(97)00457-9
更新日期：1998-01-01 00:00:00
abstract：:The interindividual variability of the hydrolysis of leucine enkephalin, and of the formation of its hydrolysis by-products has been studied in human plasma. In agreement with known data, the data obtained indicate that Leu-enkephalin is degraded by several enzymes, belonging to three classes: aminopeptidases, dipepti...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(97)00129-0
更新日期：1997-01-01 00:00:00
