Abstract:
:Mammalian vestibular type I hair cells (VIHCs) are innervated by an afferent synaptic calyx which contains vesicles and is immunoreactive for GABA. We describe here the effects of GABA on electrophysiological properties and on cytosolic free-calcium levels ([Ca2+]i) of VIHCs isolated from guinea-pig ampullae. Whole-cell tight-seal macroscopic currents recorded from VIHCs showed that 100 microM GABA induced a decrease in the outward currents elicited by depolarizing membrane potentials. These are known to comprise potassium calcium-dependent currents. This effect was mimicked by baclofen, a GABAB agonist, and was not affected by picrotoxin, a GABAA antagonist. GABA also induced an increase in the inward current elicited at hyperpolarized membrane potentials in 50% of the tested cells. Single channel recording in cell-attached patches revealed that externally applied GABA produced a decrease and an increase in the open probability of 170 pS and 45 pS and of 15 pS channels, respectively. In imaging experiments using the dye Fura-2 to measure [Ca2+]i, the only reversible modulation of [Ca2+]i observed in response to GABA application was a decrease. These results demonstrate a modulation of calcium and potassium conductances by GABA, via GABAB receptors, in guinea-pig VIHCs.
journal_name
Brain Resjournal_title
Brain researchauthors
Lapeyre PN,Kolston PJ,Ashmore JFdoi
10.1016/0006-8993(93)90882-nsubject
Has Abstractpub_date
1993-04-23 00:00:00pages
269-76issue
1-2eissn
0006-8993issn
1872-6240pii
0006-8993(93)90882-Njournal_volume
609pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Histone proteins from purified nuclei of neonatal and adult mouse retinas were analyzed and compared utilizing SDS-polyacrylamide gel electrophoresis. Identical procedures were applied to examine the histones extracted from the brains of the same animals. In the newborn and mature retina and brain, 8 histone fractions...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(87)90150-7
更新日期:1987-06-01 00:00:00
abstract::After glucocorticoid stimulation, glucocorticoid receptors (GRs) are translocated to the nucleus to modulate transcription of glucocorticoid target genes. The subcellular distribution and trafficking of GR in cultured cells has been studied quite intensively using several techniques. However, the intracellular localiz...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.03.052
更新日期:2010-05-17 00:00:00
abstract::The selective dopaminergic antagonist ligands [3H]SCH 23390 and [3H]sulpiride were used to reveal autoradiographically dopamine D1 and D2 receptors, respectively, in brain sections from rats which had received unilateral 6-hydroxydopamine (6-OHDA) injections destroying ascending nigrostriatal neurones. The binding of ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)90439-i
更新日期:1990-04-23 00:00:00
abstract::An in vitro model was used to study the cytokinetics of astroglial cells derived from neonatal rat cerebellum. Confluent monolayers of astrocytes (85% astroglial as assessed by GFAP immunoreactivity) were subcultured at low cell density and after 2-3 days growth were rendered quiescent by shifting them to low serum me...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91485-0
更新日期:1988-01-26 00:00:00
abstract::The axons in the nerve fiber layer (NFL) of the adult rat retina were examined by transmission electron microscopy. NFL axons range in size from 0.12 to about 2.0 microm, with a peak at 0.3-0.4 microm. In addition to conventional small mitochondria in the NFL axons contain some large ones, which are similar to astrocy...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)91222-2
更新日期:1983-01-03 00:00:00
abstract::Slow negative potentials, evoked by stimulation of the lumbar dorsal roots, have been demonstrated in the dorsal horn of an isolated, hemisected spinal cord preparation from golden hamsters. Paired stimuli revealed a period of partial suppression of this slow potential persisting for up to 2 s following the conditioni...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90940-4
更新日期:1982-02-04 00:00:00
abstract::Previous work has shown that bilateral injection of as little as 10 microgram of cycloheximide (CHX) into the amygdala, but not into the internal capsule, caused a time-dependent disruption of long-term retention of passive avoidance training. Under these conditions, protein synthesis in the entire brain was inhibited...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)91178-1
更新日期:1981-03-23 00:00:00
abstract::Phosphocreatine-Mg-complex acetate (PCr-Mg-CPLX) is a creatine-derived compound that in previous in vitro research was able to increase neuronal creatine independently of the creatine transporter, thus providing hope to cure the hereditary syndrome of creatine transporter deficiency. In previous research we showed tha...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.06.009
更新日期:2009-08-18 00:00:00
abstract::The patch-clamp technique was used to characterize the single channel ion currents in primary cultures of rat astrocytes. The most dominant channel type, which was found in over half of the inside-out membrane patches, was a potassium channel. The measured reversal potential was -67 mV, which is close to the calculate...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90360-0
更新日期:1989-04-10 00:00:00
abstract::Repetitive generation of spreading depression (SD) waves may induce metabolic and functional disturbances in the invaded brain regions. In order to better characterize this state, up to 60 SD waves were elicited in the cerebral cortex of anesthetized rats by intracortical injections of 2 microliters of isotonic K-acet...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91399-0
更新日期:1987-02-24 00:00:00
abstract::Intraventricular injections of kainic acid were used to destroy the hippocampal CA3-CA4 cells, thus denervating the inner third of the molecular layer of the fascia dentata and stratum radiatum and stratum oriens of area CA1. The responses of intact afferents to such lesions were then examined histologically. The hipp...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(80)90825-2
更新日期:1980-01-20 00:00:00
abstract::Apoptosis has been shown to be induced by some pathological stimuli. MPP+ is a neurotoxin and an inducer of parkinsonism. When SH-SY5Y cells, human neuroblastoma cell line, were treated with MPP+, cell death estimated by lactate dehydrogenase (LDH) leakage assay occurred. The cell death was associated with the DNA fra...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)01120-x
更新日期:1995-12-18 00:00:00
abstract::The cataleptic activity of morphine and methadone was markedly potentiated in frontally decorticated rats with no apparent changes in the onset or duration of action. Enhancement of the opioid cataleptic response was not due to changes in the availability of the drugs in the brain. The potentiation of methadone-induce...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91028-1
更新日期:1988-05-24 00:00:00
abstract::Diabetes mellitus (DM) may lead to gastrointestinal motility disorders. Rodent models of DM indicate the presence of morpho-functional abnormalities of the enteric nervous system. Here, we evaluated whether experimental DM can cause changes in the excitatory cholinergic fibers, neuronal density, and voltage-gated sodi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2018.11.041
更新日期:2019-04-01 00:00:00
abstract::Cytochrome aa3 (cyt aa3) is the main catalyst of cellular oxygen consumption. The properties of cyt aa3 will define the tissue oxygen requirements and provide an insight into energy supply and demand. Currently dual-wavelength (605-590 or 605-620 nm) reflectance spectrophotometry is used to monitor cyt aa3 redox state...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(85)91236-3
更新日期:1985-12-23 00:00:00
abstract::The muscarinic cholinergic receptor of rat eccrine sweat gland was characterized using quantitative autoradiography and [3H]QNB as radioligand. The distribution of radioligand was maximal in the secretory coil. Autoradiographic competition binding studies were performed using selective antagonists to M1 (pirenzepine),...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90415-r
更新日期:1991-05-31 00:00:00
abstract::alpha-Bungarotoxin (alpha-BuTX) binds in a saturable and practically irreversible fashion to membrane-associated receptors in the ciliary ganglion of the adult chick. The binding of toxin to receptors is competitively inhibited by nicotinic cholinergic ligands, and for these properties the receptors are regarded as ac...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(78)91130-7
更新日期:1978-09-15 00:00:00
abstract::After-effects of fusimotor action on dynamic sensitivity of spindle primary afferents from soleus resulted from low rate stimulation of both static and dynamic gamma-axons. Sensitivity to small sinusoidal stretches superimposed on triangles was enhanced during triangular lengthening but not during shortening. These af...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)90289-x
更新日期:1982-01-28 00:00:00
abstract::Mild NMDA receptor activation is correlated with neuroprotection in models of cerebral ischemia. Neuroprotection with NMDA manifests as a form of ischemic tolerance and involves the induction of cellular stress systems sensitive to disturbances in cellular calcium homeostasis. Unilateral micro-injection of 10, 160 and...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.10.014
更新日期:2009-01-09 00:00:00
abstract::Effects of intracranial self-stimulation of central 'punishment areas' were studied on an operant conditioning of vertical eye movements in the midpontine pretrigeminal cats as well as in the encéphale isolé cats. In 36 pretrigeminal cats, the ventromedial hypothalamus (VMH), basal amygdaloid nuclei (AMY), dorsal cent...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)91008-8
更新日期:1981-12-21 00:00:00
abstract::We have recently reported that the burst firing neurons are found in layer III as well as in layer V of cat sensorimotor cortex in vitro. In the present study, we examined the synaptic excitability of layer III neurons by white matter stimulation and compared with their firing patterns against the current injections t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01850-8
更新日期:1999-09-18 00:00:00
abstract::This work examines the age-related changes of the NO pathway in the central nervous system (CNS), analyzing nitric oxide synthase (NOS) isoform expression, the level of nitrotyrosine-modified proteins, and the NOS activity in the cerebral cortex, decorticated brain (basal ganglia, thalamus, hypothalamus, tegtum and te...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)03575-8
更新日期:2002-11-29 00:00:00
abstract::Two distinct families of low-molecular-weight toxins (argiotoxins) have been isolated from the venom of the orb-web spider. Argiope trifasciata. The toxins have been purified to homogeneity and characterised by spectroscopic, mass spectrometric and microchemical analysis. The major biologically active member of the fi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91098-0
更新日期:1988-05-10 00:00:00
abstract::This study investigated the effects of REM (rapid eye movement) sleep deprivation (RSD) on the activity of central cholinergic receptors and passive avoidance retention in rats treated neonatally with clomipramine. Male rat pups treated with clomipramine (15 mg/kg, s.c.) twice daily from postnatal day 5 to 21 were sub...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02248-4
更新日期:2000-06-09 00:00:00
abstract::The present study investigated the hypothesis that social isolation increases aggression by increasing the number of V1a vasopressin receptors in the anterior hypothalamus (AH). Male hamsters were randomly assigned to a group that was allowed to interact with a small nonaggressive hamster three times each week for 3 w...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.12.081
更新日期:2006-02-16 00:00:00
abstract::Protein kinase C (PKC) activity was measured in rat brain with 2 h of middle cerebral artery (MCA) and common carotid artery (CCA) occlusion, using dual autoradiography of [14C]iodoantipyrine (IAP) and [3H]phorbol-12,13-dibutyrate (PDBu). In the ischemic brain, it required more than 120 min of incubation to obtain a p...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)01342-x
更新日期:1997-03-07 00:00:00
abstract::We previously reported that angiotensin II type 2 (AT(2)) receptor signaling prevents neural damage and cognitive impairment after focal cerebral ischemia. We investigated the possible roles of the AT(2) receptor in the sex difference, focusing on cognitive function and ischemic brain damage using AT(2) receptor-defic...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.08.068
更新日期:2009-12-01 00:00:00
abstract::Effects of methamphetamine (MAP) on the extracellular neuropeptide Y (NPY) and noradrenaline (NA) levels were examined in the vicinity of the paraventricular nucleus (PVN) of freely moving rats by means of push-pull perfusion. The NA and NPY levels increased significantly in 30-60 min and reached the maximum level in ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)01343-1
更新日期:1996-01-22 00:00:00
abstract::Intracisternal (i.c.) administration of the glutamate-receptor antagonist kynurenate to halothane-anesthetized rats (paralyzed, ventilated) produced an initial hypertension associated with an increase in lumbar sympathetic nerve discharge. Kynurenate (i.c.) blocked or greatly reduced all sympathetic reflexes investiga...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91320-0
更新日期:1988-01-12 00:00:00
abstract::The hypothesis according to which BoTx and TeTx block neuromuscular transmission by impairing Ca2+ influx was tested by recording presynaptic membrane currents at motor endplates of the mouse by means of external electrodes. The use of K-channel blockers allowed us to observe inward Ca current which was unaffected by ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90617-0
更新日期:1983-07-04 00:00:00