Intracellular polyamine pools, oligopeptide-binding protein A expression, and resistance to aminoglycosides in Escherichia coli.

Abstract:

:The role of intracellular free polyamine (putrescine and spermidine) pools in multiple resistance to aminoglycoside antibiotics was investigated among in vitro selected kanamycin-resistant Escherichia coli J53 mutants expressing diminished oligopeptide-binding protein (OppA) levels and/or defective ornithine decarboxylase (ODC) activity. The results suggest that diminished OppA content, but not defective ODC activity expression, increased the relative concentration of free spermidine as compared to the wild type strain. Moreover, by adding exogenous polyamines or polyamine synthesis inhibitors to cultures with different mutant strains, a direct relationship between the intracellular OppA levels and resistance to kanamycin was revealed. Collectively these results further suggest a complex relation among OppA expression, aminoglycoside resistance and polyamine metabolism.

journal_name

Mem Inst Oswaldo Cruz

authors

Acosta MB,Ferreira RC,Ferreira LC,Costa SO

doi

10.1590/s0074-02762005000700020

subject

Has Abstract

pub_date

2005-11-01 00:00:00

pages

789-93

issue

7

eissn

0074-0276

issn

1678-8060

pii

S0074-02762005000700020

journal_volume

100

pub_type

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