Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre.

Abstract:

:The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.

journal_name

Mem Inst Oswaldo Cruz

authors

Fagundes A,Schubach A,Paula CC,Bogio A,Antonio Lde F,Schiavoni PB,Monteiro Vde S,Madeira Mde F,Quintella LP,Valete-Rosalino CM,Vasconcellos Ede C,Azeredo-Coutinho RB,Pacheco RS,Marzochi MC,Marzochi KB

doi

10.1590/s0074-02762010000100018

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

109-12

issue

1

eissn

0074-0276

issn

1678-8060

pii

S0074-02762010000100018

journal_volume

105

pub_type

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