Reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures.

Abstract:

:This study aimed to correlate the presence of ica genes, biofilm formation and antimicrobial resistance in 107 strains of Staphylococcus epidermidis isolated from blood cultures. The isolates were analysed to determine their methicillin resistance, staphylococcal cassette chromosome mec (SCCmec) type, ica genes and biofilm formation and the vancomycin minimum inhibitory concentration (MIC) was measured for isolates and subpopulations growing on vancomycin screen agar. The mecA gene was detected in 81.3% of the S. epidermidis isolated and 48.2% carried SCCmec type III. The complete icaADBC operon was observed in 38.3% of the isolates; of these, 58.5% produced a biofilm. Furthermore, 47.7% of the isolates grew on vancomycin screen agar, with an increase in the MIC in 75.9% of the isolates. Determination of the MIC of subpopulations revealed that 64.7% had an MIC ≥ 4 μg mL-1, including 15.7% with an MIC of 8 μg mL-1 and 2% with an MIC of 16 μg mL-1. The presence of the icaADBC operon, biofilm production and reduced susceptibility to vancomycin were associated with methicillin resistance. This study reveals a high level of methicillin resistance, biofilm formation and reduced susceptibility to vancomycin in subpopulations of S. epidermidis. These findings may explain the selection of multidrug-resistant isolates in hospital settings and the consequent failure of antimicrobial treatment.

journal_name

Mem Inst Oswaldo Cruz

authors

Pinheiro L,Brito CI,Pereira VC,Oliveira Ad,Camargo CH,Cunha Mde L

doi

10.1590/0074-0276140120

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

871-8

issue

7

eissn

0074-0276

issn

1678-8060

pii

S0074-02762014000700871

journal_volume

109

pub_type

杂志文章
  • Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells.

    abstract::Acute infection with Trypanosoma cruzi results in intense myocarditis, which progresses to a chronic, asymptomatic indeterminate form. The evolution toward this chronic cardiac form occurs in approximately 30% of all cases of T. cruzi infection. Suppression of delayed type hypersensitivity (DTH) has been proposed as a...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276108062013004

    authors: Thé TS,Portella RS,Guerreiro ML,Andrade SG

    更新日期:2013-09-01 00:00:00

  • A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi.

    abstract::We report the molecular characterization of a novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi. Steady-state level of transcripts of this sequence family appeared to be developmentally regulated, since only in the replicative forms the parasite sh...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000100016

    authors: Verbisck NV,dos Santos MR,Engman DM,Angel Chiurillo M,Ramirez JL,Araya JE,Mortara RA,da Silveira JF

    更新日期:2003-01-01 00:00:00

  • Detection and characterization of rotavirus G and P types from children participating in a rotavirus vaccine trial in Belém, Brazil.

    abstract::This study sought the characterization of rotaviruses in a trial with a tetravalent rhesus-human rotavirus vaccine in Belém, Brazil in children who received three doses of vaccine or placebo in the 1st, 3rd and 5th months of life. Rotavirus electropherotypes, subgroups, G serotypes, G, [P] and [P], G genotypes were de...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1590/s0074-02762002000100020

    authors: Mascarenhas JD,Linhares AC,Gabbay YB,Leite JP

    更新日期:2002-01-01 00:00:00

  • Benzonidazole levels in blood vary with age in rats.

    abstract::Benznidazole (Bz) exhibits toxic side effects in animal studies and clinical use. Reductive metabolism of Bz in liver microsomes modulates the duration of its chemotherapeutic effect and its toxicity. The rate of this metabolism depends on age and is less intense in newborns and youngsters than in adults. In the prese...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000300021

    authors: Bulffer RF,Castro JA,Fanelli SL

    更新日期:2011-05-01 00:00:00

  • Early Cretaceous trypanosomatids associated with fossil sand fly larvae in Burmese amber.

    abstract::Early Cretaceous flagellates with characters typical of trypanosomatids were found in the gut of sand fly larvae, as well as in surrounding debris, in Burmese amber. This discovery supports a hypothesis in which free-living trypanosomatids could have been acquired by sand fly larvae in their feeding environment and th...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000070

    authors: Poinar G Jr

    更新日期:2007-08-01 00:00:00

  • Schistosomiasis mansoni is associated with pyogenic liver abscesses in the state of Minas Gerais, Brazil.

    abstract::The association between pyogenic liver abscesses and schistosomiasis has been confirmed by clinical and experimental studies. In this retrospective study of 78 patients with pyogenic liver abscesses the association with schistosomiasis has been investigated. Pyodermitis, a known focus of bacteremia, was observed in 19...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000900021

    authors: Teixeira R,Pfeilsticker FJ,Santa Cecília GD,Nobre V,Fonseca LP,Serufo JC,Coelho PM,Lambertucci JR

    更新日期:2001-01-01 00:00:00

  • A chromosome 9 deletion in Plasmodium falciparum results in loss of cytoadherence.

    abstract::Many lines of Plasmodium falciparum undergo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and the selected cells produce gametocy...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761992000700011

    authors: Kemp DJ,Thompson J,Barnes DA,Triglia T,Karamalis F,Petersen C,Brown GV,Day KP

    更新日期:1992-01-01 00:00:00

  • ERRATUM: Structural insights into Plasmodium falciparum nicotinamide mononucleotide adenylyltransferase: oligomeric assembly".

    abstract::[This corrects the article doi: 10.1590/0074-02760180073]. ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,已发布勘误

    doi:10.1590/0074-02760180073er

    authors:

    更新日期:2018-01-01 00:00:00

  • Acute inflammatory response in the stomach of BALB/c mice challenged with coccoidal Helicobacter pylori.

    abstract::An experimental murine model was used to verify the viability and pathogenicity of coccoid Helicobacter pylori. For this purpose, 27 BALB/c mice were inoculated intragastrically with 1 ml broth culture (10(8)organisms/ml) of a coccoid H. pylori clinical isolate. The animals were divided into two groups. Nine were infe...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000800023

    authors: Rabelo-Gonçalves EM,Nishimura NF,Zeitune JM

    更新日期:2002-12-01 00:00:00

  • Sativa seeds against Schistosoma mansoni different stages.

    abstract::The schistosomicidal properties of Nigella sativa seeds were tested in vitro against Schistosoma mansoni miracidia, cercariae, and adult worms. Results indicate its strong biocidal effects against all stages of the parasite and also showed an inhibitory effect on egg-laying of adult female worms. In the present work w...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762005000200016

    authors: Mohamed AM,Metwally NM,Mahmoud SS

    更新日期:2005-04-01 00:00:00

  • Comparative developmental and susceptibility to insecticide of Bolivian and Brazilian populations of Triatoma infestans.

    abstract::The triatomine bug Triatoma infestans probably originated in Bolivia and dispersed passively over wide areas of South America, where it is the principal vector of Trypanosoma cruzi. In the region of its probable origin this species shows colonization in two different ecotopes, so that it may be encountered in sylvatic...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000600025

    authors: Pires HH,Barbosa SE,Diotaiuti L

    更新日期:2000-11-01 00:00:00

  • Merozoite surface protein 2 allelic variation influences the specific antibody response during acute malaria in individuals from a Brazilian endemic area.

    abstract::The antibody response to Plasmodium falciparum parasites of naturally infected population is critical to elucidate the role of polymorphic alleles in malaria. Thus, we evaluated the impact of antigenic diversity of repetitive and family dimorphic domains of the merozoite surface protein 2 (MSP-2) on immune response of...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000048

    authors: Sallenave-Sales S,Faria CP,Zalis MG,Daniel-Ribeiro CT,Ferreira-da-Cruz Mde F

    更新日期:2007-06-01 00:00:00

  • Uptake of macromolecules by cercariae during skin penetration and transformation to schistosomula (Schistosoma mansoni).

    abstract::Here, we observed the uptake of membrane-impermeant molecules by cercariae as they penetrate the skin and are transformed into schistosomula. We propose that membrane-impermeant molecules, Lucifer Yellow, Propidium iodide and Hoechst 33258 enter the parasite through both thenephridiopore and the surface membrane and t...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000400007

    authors: Thornhill J,Kusel J,Oliviera FA,Ribeiro F,Lima SF,Coelho PM,McVeigh P,Mattos AC

    更新日期:2010-07-01 00:00:00

  • Acute Chagas disease in El Salvador 2000-2012 - need for surveillance and control.

    abstract::Several parasitological studies carried out in El Salvador between 2000-2012 showed a higher frequency of acute cases of Chagas disease than that in other Central American countries. There is an urgent need for improved Chagas disease surveillance and vector control programs in the provinces where acute Chagas disease...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276140416

    authors: Sasagawa E,Aguilar AV,Ramírez MA,Chévez JE,Nakagawa J,Cedillos RA,Kita K

    更新日期:2014-04-01 00:00:00

  • Epidemiological characteristics of Schistosoma mansoni infection in rural and urban endemic areas of Minas Gerais, Brazil.

    abstract::To compare the epidemiological profile and socioeconomic factors associated to the infection by Schistosoma mansoni in a rural and an urban endemic area a cross-sectional study was performed in Agua Branca de Minas (rural area) and Bela Fama (urban area), both situated in the State of Minas Gerais, Brazil. Two hundred...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761997000500001

    authors: Amorim MN,Rabello A,Contreras RL,Katz N

    更新日期:1997-09-01 00:00:00

  • alpha-glycerophosphate dehydrogenase activity in flight muscles of triatomine bugs Panstrongylus megistus and Triatoma sordida.

    abstract::The alpha-glycerophosphate dehydrogenase (alpha-GPDH) activity in flight muscles of Panstrongylus megistus and Triatoma sordida, vectors of Chagas disease in Brazil, was studied. Both species showed higher enzymatic activities in fliers than in non-fliers insects. T. sordida exhibited a higher proportion of flier inse...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000500016

    authors: Soares RP,Santoro MM

    更新日期:2000-09-01 00:00:00

  • The in vitro activity of fatty diamines and amino alcohols against mixed amastigote and trypomastigote Trypanosoma cruzi forms.

    abstract::Four diamines and three amino alcohols derived from 1-decanol, 1-dodecanol and 1,2-dodecanediol were evaluated in an in vitro assay against a mixture of trypomastigote and intracellular amastigote forms of Trypanosoma cruzi. Two of these compounds (6 and 7) showed better activity against both proliferative stages of T...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276130496

    authors: Sales Júnior PA,Rezende Júnior CO,Le Hyaric M,Almeida MV,Romanha AJ

    更新日期:2014-06-01 00:00:00

  • In vitro susceptibility of Plasmodium falciparum Welch field isolates to infusions prepared from Artemisia annua L. cultivated in the Brazilian Amazon.

    abstract::Artemisinin is the active antimalarial compound obtained from the leaves of Artemisia annua L. Artemisinin, and its semi-synthetic derivatives, are the main drugs used to treat multi-drug-resistant Plasmodium falciparum (one of the human malaria parasite species). The in vitro susceptibility of P. falciparum K1 and 3d...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000700004

    authors: Silva LF,Magalhães PM,Costa MR,Alecrim Md,Chaves FC,Hidalgo Ade F,Pohlit AM,Vieira PP

    更新日期:2012-11-01 00:00:00

  • The enigmatic eosinophil: investigation of the biological role of eosinophils in parasitic helminth infection.

    abstract::In many helminth infected hosts the number of eosinophils increases dramatically, often without any concurrent increases in the number of other leukocytes, so that eosinophils become the dominant cell type. Many experimental investigations have shown that the eosinophilia is induced by interleukin-5 (IL-5) but its fun...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761997000800013

    authors: Ovington KS,Behm CA

    更新日期:1997-01-01 00:00:00

  • Triatoma jatai sp. nov. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae).

    abstract::Triatoma jatai sp. nov. is the first new species of triatomine to be described in the state of Tocantins, in the northern region of Brazil. It was caught on rock outcrops in the wild environment and, more recently, invading homes. While T. jatai sp. nov. is morphologically similar to Triatoma costalimai, it is disting...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/S0074-0276108042013006

    authors: Gonçalves TC,Teves-Neves SC,Santos-Mallet JR,Carbajal-de-la-Fuente AL,Lopes CM

    更新日期:2013-06-01 00:00:00

  • Differential regulation of granuloma size and hepatic fibrosis in schistosome infections.

    abstract::Granuloma size is the variable most frequently used to evaluate the immunopathogenesis of schistosome infections. However, hepatic fibrosis is at the least an equally relevant variable. Hepatic fibrosis and the size of circumoval granulomas are frequently dissociated in experimental murine Schistosoma mansoni and S. j...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761997000500024

    authors: Cheever AW

    更新日期:1997-09-01 00:00:00

  • On the safe use of pesticides in controlling the terrestrial mollusc pests.

    abstract::The preferred food items of the slugs Laevicaulis alte and the snails Achatina fulica were used to prepare 'poison baits' by injecting the pesticides 'Rogor' and 'Nuvan' to kill these mollusc pests. The 'poison baits' prepared with Trichosanthes dioica and Lycopersicum esculentum were accepted by 100% individuals of b...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761993000200019

    authors: Panigrahi A,Raut SK

    更新日期:1993-04-01 00:00:00

  • Widespread nasal carriage of Mycobacterium lepraeamong a healthy population in a hyperendemic region of northeastern Brazil.

    abstract::A case-control study was conducted to determine the presence ofMycobacterium lepraeDNA in nasal secretions of leprosy cases and nonleprosy individuals in Fortaleza, Brazil. It included 185 cases identified by physicians at the Dona Libânia National Reference Centre for Sanitary Dermatology (CDERM). A control group (Co...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760150178

    authors: Lima LN,Frota CC,Mota RM,Almeida RL,Pontes MA,Gonçalves Hde S,Rodrigues LC,Kendall C,Kerr L

    更新日期:2015-11-01 00:00:00

  • COVID-19: molecular targets, drug repurposing and new avenues for drug discovery.

    abstract::Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly contagious infection that may break the healthcare system of several countries. Here, we aimed at presenting a critical view of ongoing drug repurposing efforts for COVID-19 as well as discussing ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760200254

    authors: Senger MR,Evangelista TCS,Dantas RF,Santana MVDS,Gonçalves LCS,de Souza Neto LR,Ferreira SB,Silva-Junior FP

    更新日期:2020-10-02 00:00:00

  • Widespread distribution of CTX-M and plasmid-mediated AmpC β-lactamases in Escherichia coli from Brazilian chicken meat.

    abstract::The dissemination of plasmid-mediated antimicrobial resistance genes may pose a substantial public health risk. In the present work, the occurrences of blaCTX-M and plasmid-mediated ampC and qnr genes were investigated in Escherichia coli from 16 chicken carcasses produced by four commercial brands in Brazil. Of the b...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760140389

    authors: Botelho LA,Kraychete GB,Costa e Silva JL,Regis DV,Picão RC,Moreira BM,Bonelli RR

    更新日期:2015-04-01 00:00:00

  • Allelic diversity at the merozoite surface protein-1 (MSP-1) locus in natural Plasmodium falciparum populations: a brief overview.

    abstract::The merozoite surface protein-1 (MSP-1) locus of Plasmodium falciparum codes for a major asexual blood-stage antigen currently proposed as a major malaria vaccine candidate. The protein, however, shows extensive polymorphism, which may compromise its use in sub-unit vaccines. Here we compare the patterns of allelic di...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000500013

    authors: Ferreira MU,Kaneko O,Kimura M,Liu Q,Kawamoto F,Tanabe K

    更新日期:1998-09-01 00:00:00

  • Methicillin-resistant Staphylococcus aureus in human milk.

    abstract::We collected and analyzed 500 samples of human milk, from five Brazilian cities (100 from each) to detect methicillin-resistant strains of Staphylococcus aureus (MRSA) producing enterotoxins. We found 57 strains of MRSA, and the mecA gene, responsible for resistance, was detected in all of them using a specific molecu...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/S0074-02762000000100003

    authors: Novak FR,Almeida JA,Warnken MB,Ferreira-Carvalho BT,Hagler AN

    更新日期:2000-01-01 00:00:00

  • Acanthocefalan eggs in animal coprolites from archaeological sites from Brazil.

    abstract::An important point in paleoparasitology is the correct diagnosis of the origin of coprolites found in archaeological sites. The identification of human and animal coprolites, through the study of the shape, size, characteristics after rehydration, alimentary contents, and the presence of parasites, has proved to be ac...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761989000200007

    authors: Ferreira LF,Araújo A,Confalonieri U,Chame M

    更新日期:1989-04-01 00:00:00

  • Cryptosporidium sp. in children suffering from acute diarrhea at Uberlândia City, State of Minas Gerais, Brazil.

    abstract::This study's objective was to search for Cryptosporidium sp. in diarrheic feces from children aged zero to 12 years and cared for at medical units within Universidade Federal de Uberlândia or at a private practice in Uberlândia, State of Minas Gerais, Brazil, from September 1992 to August 1993. Three fecal samples pre...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761996000500003

    authors: Gennari-Cardoso ML,Costa-Cruz JM,de Castro E,Lima LM,Prudente DV

    更新日期:1996-09-01 00:00:00

  • Electrocardiographic alteration among first degree relatives with serologic evidence of Trypanosoma cruzi infection. A sibship study.

    abstract::To analyze whether electrocardiographic alterations (ECGA) in patients with antibodies to Trypanosoma cruzi showed a pattern of familial aggregation, a sample of 379 young adults (166 men and 213 women) distributed in sibships, were assessed for the presence of anti-T. cruzi antibodies, and subjected to a complete cli...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761994000300015

    authors: Morini JC,Berra H,Dávila HO,Pividori JF,Bottasso OA

    更新日期:1994-07-01 00:00:00