Detection and characterization of rotavirus G and P types from children participating in a rotavirus vaccine trial in Belém, Brazil.

Abstract:

:This study sought the characterization of rotaviruses in a trial with a tetravalent rhesus-human rotavirus vaccine in Belém, Brazil in children who received three doses of vaccine or placebo in the 1st, 3rd and 5th months of life. Rotavirus electropherotypes, subgroups, G serotypes, G, [P] and [P], G genotypes were determined in 93.3%, 95.9%, 93.3%, 73.3%, 95.5% and 92.2% of isolates, respectively. Serotypes G1, G2 and G4 were detected in 58.9%, 30% and 4.4% of the cases, respectively. Rotavirus genotype G5 was detected for the first time in Northern region in 4.4% of the infections. Rotavirus genotypes P[8], P[4], P[6] and P[8 + 6] were detected in 54.5%, 26.7%, 12.2%, and 2.2% of the cases, respectively. The predominant genotypes were P[8], G1 and P[4], G2 with 53% and 26.6% of the infections, respectively. Unusual strains accounted for 20.5% including P[4], G1, P[6], G1, P[6], G4, P[6], G5, P[8], G2, P[8], G5. Mixed infections involving P[8 + 6], G2 and P[8 + 6], G1 were also noted. The neonatal P[6] strains associated with diarrhea were detected among children aged 9-24 months. To our knowledge, this study represents the first in Brazil to analyse, on molecular basis, rotavirus genotypes from children participating in a rotavirus vaccine trial. These results are of potential importance regarding future rotavirus vaccination strategies in Brazil.

journal_name

Mem Inst Oswaldo Cruz

authors

Mascarenhas JD,Linhares AC,Gabbay YB,Leite JP

doi

10.1590/s0074-02762002000100020

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

113-7

issue

1

eissn

0074-0276

issn

1678-8060

pii

S0074-02762002000100020

journal_volume

97

pub_type

临床试验,杂志文章,随机对照试验
  • Age structure and abundance in populations of muscoid flies from a poultry facility in Southeast Brazil.

    abstract::Muscina stabulans, M. domestica, Chrysomya putoria, C. megacephala and Stomoxys calcitrans were the most abundant muscoid flies captured in a poultry facility in southeastern Brazil. We examined the gonadotrophic profiles of the females caught at different sites and different times and found that Mu. stabulans and M. ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000200022

    authors: Avancini RM,Silveira GA

    更新日期:2000-03-01 00:00:00

  • Comparison of some molecular-genetic techniques for identification of Leishmania circulating in natural foci of zoonotic cutaneous leishmaniasis in the central Asia region.

    abstract::Different molecular-genetic methods were used to identify a cohort of Leishmania strains from natural foci of zoonotic cutaneous leishmaniasis located in Central Asia, on the former USSR territory. The results obtained using isoenzymes, PCR, restriction fragment length polymorphisms of kDNA and molecular hybridization...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761997000100023

    authors: Strelkova MV,Pacheco RS,Bulat SA,Rakitskaya TA

    更新日期:1997-01-01 00:00:00

  • Monitoring the circulation of rotavirus among children after the introduction of the Rotarix™ vaccine in Goiânia, Brazil.

    abstract::The epidemiological features of rotavirus A (RVA) infection differ between children from developing and developed countries which could result in differences in vaccine efficacy around the world. To evaluate the impact of Rotarix™ on RVA prevalence, we monitored RVA genotypes circulating in Goiânia by monitoring virus...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000400018

    authors: Borges AM,Dias e Souza M,Fiaccadori FS,Cardoso Dd

    更新日期:2011-06-01 00:00:00

  • Evaluation of the direct agglutination test and the rK39 dipstick test for the sero-diagnosis of visceral leishmaniasis.

    abstract::The direct agglutination test (DAT) based on a freeze-dried antigen and the rK39 dipstick test were evaluated for the sero-diagnosis of visceral leishmaniasis (VL). The sensitivity and specificity of both tests were determined using sera from confirmed VL patients (n = 21), healthy controls (n = 19) and from patients ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000700015

    authors: Schallig HD,Canto-Cavalheiro M,da Silva ES

    更新日期:2002-10-01 00:00:00

  • Lutzomyia diamantinensis sp. nov., a new phlebotomine species (Diptera: Psychodidae) from a quartzite cave in Diamantina, state of Minas Gerais, Brazil.

    abstract::A new species of Brazilian phlebotomine sandfly found in Brazil, municipality of Diamantina, state of Minas Gerais, is described based on males and females collected in a quartzite cave. The body of spermathecae is continuous to the individual duct, lanky and tapering at the end, with conical shaped, not striated and ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000800007

    authors: Barata RA,Serra e Meira PC,Carvalho GM

    更新日期:2012-12-01 00:00:00

  • New insights into trypanosomatid U5 small nuclear ribonucleoproteins.

    abstract::Several protozoan parasites exist in the Trypanosomatidae family, including various agents of human diseases. Multiple lines of evidence suggest that important differences are present between the translational and mRNA processing (trans splicing) systems of trypanosomatids and other eukaryotes. In this context, certai...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000200003

    authors: Silva MT,Ambrósio DL,Trevelin CC,Watanabe TF,Laure HJ,Greene LJ,Rosa JC,Valentini SR,Cicarelli RM

    更新日期:2011-03-01 00:00:00

  • Occurence of larval Culicidae (Diptera) in water retained in Aquascypha hydrophora (Fungus: Stereaceae) in Central Amazônia, Brazil.

    abstract::The community structure of insects, especially mosquito larvae, in water held in the fungus Aquascypha hydrophora (Berk.) Reid (Stereaceae) is reported. The study was done in the Adolpho Ducke Forest Reserve, 26 km east of Manaus, AM, Brazil, from September 1998 through November 1999. The most abundant entomofauna wer...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000800023

    authors: Ferreira RL,Oliveira AF,Pereira ES,Hamada N

    更新日期:2001-11-01 00:00:00

  • Development of a novel plaque reduction neutralisation test for hantavirus infection.

    abstract::In the Americas, hantaviruses cause severe cardiopulmonary syndrome (HCPS) with a high fatality rate. Hantavirus infection is commonly diagnosed using serologic techniques and reverse transcription-polymerase chain reaction. This paper presents a novel plaque reduction neutralisation test (PRNT) for detecting antibodi...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760150102

    authors: Pádua Md,Souza WM,Lauretti F,Figueiredo LT

    更新日期:2015-08-01 00:00:00

  • Evolutionary histories of expanded peptidase families in Schistosoma mansoni.

    abstract::Schistosoma mansoni is one of the three main causative agents of human schistosomiasis, a major health problem with a vast socio-economic impact. Recent advances in the proteomic analysis of schistosomes have revealed that peptidases are the main virulence factors involved in the pathogenesis of this disease. In this ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000700013

    authors: Silva LL,Marcet-Houben M,Zerlotini A,Gabaldón T,Oliveira G,Nahum LA

    更新日期:2011-11-01 00:00:00

  • Intestinal coccidia in Cuban pediatric patients with diarrhea.

    abstract::From May to August 1999, we evaluated 401 patients from a pediatric hospital of Havana City. One group was composed of 113 patients with diarrhea admitted to the Gastroenterology ward and a second consisted of 288 patients without diarrhea, admitted for other reasons, and hospitalized within the same time period. Thre...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000400021

    authors: Núñez FA,González OM,González I,Escobedo AA,Cordoví RA

    更新日期:2003-06-01 00:00:00

  • Molecular comparison of topotypic specimens confirms Anopheles (Nyssorhynchus) dunhami Causey (Diptera: Culicidae) in the Colombian Amazon.

    abstract::The presence of Anopheles (Nyssorhynchus) dunhami Causey in Colombia (Department of Amazonas) is confirmed for the first time through direct comparison of mtDNA cytochrome c oxidase I (COI) barcodes and nuclear rDNA second internal transcribed spacer (ITS2) sequences with topotypic specimens of An. dunhami from Tefé, ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000700010

    authors: Ruiz F,Linton YM,Ponsonby DJ,Conn JE,Herrera M,Quiñones ML,Vélez ID,Wilkerson RC

    更新日期:2010-11-01 00:00:00

  • Acquired and congenital ocular toxoplasmosis experimentally induced in Calomys callosus (Rodentia, Cricetidae).

    abstract::An experimental model for acquired and congenital ocular toxoplasmosis as well as a model to induce experimental autoimmune uveitis (EAU) was investigated in Calomys callosus. Toxoplasma gondii, ME-49 strain, was used to infect males and pregnant- and not pregnant-females while S-antigen, a major glycoprotein of the r...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761999000100021

    authors: Pereira Mde F,Silva DA,Ferro EA,Mineo JR

    更新日期:1999-01-01 00:00:00

  • Early Cretaceous trypanosomatids associated with fossil sand fly larvae in Burmese amber.

    abstract::Early Cretaceous flagellates with characters typical of trypanosomatids were found in the gut of sand fly larvae, as well as in surrounding debris, in Burmese amber. This discovery supports a hypothesis in which free-living trypanosomatids could have been acquired by sand fly larvae in their feeding environment and th...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000070

    authors: Poinar G Jr

    更新日期:2007-08-01 00:00:00

  • Increased levels of reactive oxygen species in platelets and platelet-derived microparticles and the risk of respiratory failure in HIV/AIDS patients.

    abstract::Respiratory failure (RF) is the main cause of hospital admission in HIV/AIDS patients. This study assessed comorbidities and laboratory parameters in HIV/AIDS inpatients with RF (N = 58) in relation to those without RF (N = 36). Tuberculosis showed a huge relative risk and platelet counts were slightly higher in HIV/A...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760200082

    authors: Gama WM,Oliveira LB,Chaves YO,Ribeiro F,Almeida TVR,Baptista BJA,Santana MF,Ferreira LC,Lacerda MVG,Nogueira PA

    更新日期:2020-09-14 00:00:00

  • The use of polyvinyl alcohol glutaraldehyde as solid-phase in ELISA for plague.

    abstract::Discs of polyvinyl alcohol cross-linked with glutaraldehyde were synthesized under acid catalysis (H2SO4). Then, the antigen F1 purified from Yersinia pestis was covalently linked to this modified polymer. Afterwards, an enzyme-linked immunosorbent assay (ELISA) was established for the diagnosis of plague in rabbit an...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761996000200014

    authors: Araujo AM,Petribú AT,Sales Barbosa GH,Diniz JR,de Almeida AM,Mendes Azevedo W,Malagueño E,Carvalho Júnior LB

    更新日期:1996-03-01 00:00:00

  • A checklist of arthropods associated with pig carrion and human corpses in southeastern brazil.

    abstract::Necrophagous insects, mainly Diptera and Coleoptera, are attracted to specific stages of carcass decomposition, in a process of faunistic succession. They are very important in estimating the postmortem interval, the time interval between the death and the discovery of the body. In studies done with pig carcasses expo...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000100023

    authors: Carvalho LM,Thyssen PJ,Linhares AX,Palhares FA

    更新日期:2000-01-01 00:00:00

  • Treatment of human acute schistosomiasis with oxamniquine induces an increase in interferon-gamma response to Schistosoma mansoni antigens.

    abstract::Patients with acute schistosomiasis were studied before and after oxamniquine treatment. They had been exposed to cercariae 5 to 9 weeks before, and presented compatible clinical manifestations, eosinophilia, and high levels of total IgE. Interferon-gamma (IFN-gamma) and interleukin-4 were measured by ELISA in whole b...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000002

    authors: de Souza JR,Morais CN,Aroucha ML,Miranda PJ,Barbosa CS,Domingues AL,Carvalho Júnior LB,Abath FG,Montenegro SM

    更新日期:2007-05-01 00:00:00

  • Analysis of E2 gene integrity in HPV16 and HPV58 viruses isolated from women with cervical pathology.

    abstract::Integration of human papillomavirus (HPV) DNA into human cells accompanied by the disruption of the viral genome has been described as a prerequisite for cancer development. This study aimed to investigate E2 gene integrity of HPV16 and HPV58 viruses isolated from infected women with cervical lesions. Forty-two HPV16-...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160269

    authors: González-Losa MD,Puerto-Solis M,Tenorio Ruiz J,Rosado-López AI,Hau-Aviles O,Ayora-Talavera G,Cisneros-Cutz I,Conde-Ferráez L

    更新日期:2016-12-01 00:00:00

  • Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region.

    abstract::We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000600016

    authors: Tang TH,López-Vélez R,Lanza M,Shelley AJ,Rubio JM,Luz SL

    更新日期:2010-09-01 00:00:00

  • Human toxocariasis: a seroepidemiological survey in schoolchildren of Sorocaba, Brazil.

    abstract::A seroepidemiological survey for toxocariasis, among 180 schoolchildren of the public schools of Sorocaba City, state of São Paulo, Brazil, was carried out from August 2000 to July 2001. ELISA test was performed using excretory and secretory antigens for the detection of IgG anti-Toxocara antibodies. Information regar...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:/S0074-02762004000600002

    authors: Coelho LM,Silva MV,Dini CY,Giacon Neto AA,Novo NF,Silveira EP

    更新日期:2004-10-01 00:00:00

  • First report on the presence of Biomphalaria straminea in the municipality of Jaboticatubas, State of Minas Gerais, Brazil.

    abstract::This is the first report on occurrence of Biomphalaria straminea in the district of São José de Almeida (municipality of Jaboticatubas) State of Minas Gerais, Brazil. The presence of B. glabrata and B. tenagophila had already been reported in this area. Such municipality is part of the metropolitan region of Belo Hori...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000900007

    authors: Massara CL,Carvalho Odos S,Caldeira RL,Jannotti-Passos LK,Schall VT

    更新日期:2002-01-01 00:00:00

  • A cladistic analysis of Inaequalium (Coscarón & Wygodzinsky, 1984), with information on geographical distribution (Diptera: Simuliidae).

    abstract::The black flies of the genus Inaequalium present a Neotropical distribution, with Panama at the northern limit, and the Argentinian pampas at the southern, but do not occur in the Central Amazon. This study offers a cladistic analysis establishing a hypothesis of relationships between the species of Inaequalium. A tot...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000100010

    authors: Strieder MN,Py-Daniel V

    更新日期:2002-01-01 00:00:00

  • Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections.

    abstract::An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a pr...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760140457

    authors: Bentes GA,Lanzarini NM,Lima LR,Manso PP,da Silva Ados S,Mouta Junior Sda S,Guimarães JR,de Moraes MT,Pelajo-Machado M,Pinto MA

    更新日期:2015-06-01 00:00:00

  • Diversity of Campylobacter isolates from three activated sludge systems.

    abstract::Thermophilic campylobacters were isolated from three sewage plants in Rio de Janeiro, RJ, Brazil and identified. Laboratory analysis of 390 sewage samples showed the presence of 169 thermophilic strains. The results demonstrated that human and animal pathogenic biotypes could be isolated from activated sludge during t...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000300003

    authors: Lauria-Filgueiras AL,Hofer E

    更新日期:1998-05-01 00:00:00

  • The London School of Hygiene and Tropical Medicine: a new century of malaria research.

    abstract::The global malaria situation has scarcely improved in the last 100 years, despite major advances in our knowledge of the basic biology, epidemiology and clinical basis of the disease. Effective malaria control, leading to a significant decrease in the morbidity and mortality attributable to malaria, will require a mul...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000700004

    authors: Riley EM

    更新日期:2000-01-01 00:00:00

  • Emergence of Trichosporon mycotoxinivorans (Apiotrichum mycotoxinivorans) invasive infections in Latin America.

    abstract::We report the first two cases of Trichosporon mycotoxinivorans infections in Latin America. We also conducted a literature review and a microbiological investigation, including that of clinical and environmental isolates. A 30-year-old man with chronic renal failure had disseminated infection after dialysis and a 15-y...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760170011

    authors: Almeida JN Jr,Francisco EC,Barberino MGMA,Silva LVRFD Filho,Brandão OM,Colombo AL,Padovan ACB

    更新日期:2017-10-01 00:00:00

  • Genotyping of gastroenteric viruses in hospitalised children: first report of norovirus GII.21 in Brazil.

    abstract::This retrospective study (April-September 2003) was designed to investigate the roles of the main viruses responsible for cases of acute infantile gastroenteritis in hospitalised children up to two years of age. The viruses were identified in 64.7% (88/136) of the cases and the detection rates of rotavirus A (RVA), no...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000800017

    authors: Ferreira MS,Cubel Garcia Rde C,Xavier Mda P,Ribeiro RL,Assis RM,Mota Mdo C,Leite JP,Miagostovich MP,de Oliveira SA

    更新日期:2012-12-01 00:00:00

  • Complete and de novo assembly of the Leishmania braziliensis (M2904) genome.

    abstract::Leishmania braziliensis is the etiological agent of American mucosal leishmaniasis, one of the most severe clinical forms of leishmaniasis. Here, we report the assembly of the L. braziliensis (M2904) genome into 35 continuous chromosomes. Also, the annotation of 8395 genes is provided. The public availability of this ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760180438

    authors: González-de la Fuente S,Camacho E,Peiró-Pastor R,Rastrojo A,Carrasco-Ramiro F,Aguado B,Requena JM

    更新日期:2018-12-10 00:00:00

  • Temporal and spatial distribution of Leishmania mexicana infections in a population of Neotoma micropus.

    abstract::A 19-month mark-release-recapture study of Neotoma micropus with sequential screening for Leishmania mexicana was conducted in Bexar County, Texas, USA. The overall prevalence rate was 14.7% and the seasonal prevalence rates ranged from 3.8 to 26.7%. Nine incident cases were detected, giving an incidence rate of 15.5/...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000200002

    authors: Raymond RW,McHugh CP,Witt LR,Kerr SF

    更新日期:2003-03-01 00:00:00

  • Integrated genetic epidemiology of infectious diseases: the Chagas model.

    abstract::Genetic typing of pathogenic agents and of vectors has known impressive developments in the last 10 years, thanks to the progresses of molecular biology, and to the contribution of the concepts of evolutionary genetics. Moreover, we know more and more on the genetic susceptibility of man to infectious diseases. I prop...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000500003

    authors: Tibayrenc M

    更新日期:1998-09-01 00:00:00