Comparison of some molecular-genetic techniques for identification of Leishmania circulating in natural foci of zoonotic cutaneous leishmaniasis in the central Asia region.

Abstract:

:Different molecular-genetic methods were used to identify a cohort of Leishmania strains from natural foci of zoonotic cutaneous leishmaniasis located in Central Asia, on the former USSR territory. The results obtained using isoenzymes, PCR, restriction fragment length polymorphisms of kDNA and molecular hybridization techniques are discussed in terms of their applicability, discrimination power and feasibility for answering questions related to molecular epidemiological research and for detecting mixed Leishmania infections.

journal_name

Mem Inst Oswaldo Cruz

authors

Strelkova MV,Pacheco RS,Bulat SA,Rakitskaya TA

doi

10.1590/s0074-02761997000100023

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

109-14

issue

1

eissn

0074-0276

issn

1678-8060

pii

S0074-02761997000100023

journal_volume

92

pub_type

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