Survival of tubercle bacilli in heat-fixed and stained sputum smears.

Abstract:

:We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis.

journal_name

Mem Inst Oswaldo Cruz

authors

Cardoso CL,Giacomelli LR,Helbel C,Sant'Ana JJ,Martins FM,Barreto AM

doi

10.1590/s0074-02762001000200024

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

277-80

issue

2

eissn

0074-0276

issn

1678-8060

pii

S0074-02762001000200024

journal_volume

96

pub_type

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