Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats.

Abstract:

:More sensitive methodologies are necessary to improve strongyloidiasis diagnosis. This study compared the sensitivities of the McMaster modified technique and polymerase chain reaction (PCR) assays, both performed in faecal samples. Lewis rats were subcutaneously infected with 4,000, 400 or 40 infective third-stage larvae, considered as high, moderate or low infection, respectively. Seven days later, they were euthanized to count adult nematodes recovered from the small intestine. Stool samples were used to count the number of eggs per gram (EPG) of faeces and to detect parasite DNA by PCR performed with a species and a genus primer pair. The sensitivity of these assays depended upon parasite burden and the primer specificity. All assays presented 100% sensitivity at the highest parasite load. In the moderate infection, EPG and PCR with the genus primer maintained 100% specificity, whereas PCR sensitivity with the species primer decreased to 77.7%. In low infection, the sensitivity was 60% for EPG, 0% for PCR with the species primer and 90% for PCR done with the genus primer. Together, these results suggest that PCR with a genus primer can be a very sensitive methodology to detect Strongyloides venezuelensisin faeces of Lewis rats infected with very low parasite burden.

journal_name

Mem Inst Oswaldo Cruz

authors

Marra NM,Chiuso-Minicucci F,Machado GC,Zorzella-Pezavento SF,França TG,Ishikawa LL,Amarante AF,Sartori A,Amarante MR

doi

10.1590/s0074-02762010000100008

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

57-61

issue

1

eissn

0074-0276

issn

1678-8060

pii

S0074-02762010000100008

journal_volume

105

pub_type

杂志文章
  • Phenotypic, functional, and quantitative characterization of canine peripheral blood monocyte-derived macrophages.

    abstract::The yield as well as phenotypic and functional parameters of canine peripheral blood monocyte-derived macrophages were analyzed. The cells that remained adherent to Teflon after 10 days of culture had high phagocytic activity when inoculated with Leishmania chagasi. Flow cytometric analysis demonstrated that more than...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762005000500012

    authors: Bueno R,Mello MN,Menezes CA,Dutra WO,Santos RL

    更新日期:2005-08-01 00:00:00

  • Mapping of courses on vector biology and vector-borne diseases systems: time for a worldwide effort.

    abstract::Major emergency efforts are being mounted for each vector-borne disease epidemiological crisis anew, while knowledge about the biology of arthropods vectors is dwindling slowly but continuously, as is the number of field entomologists. The discrepancy between the rates of production of knowledge and its use and need f...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160295

    authors: Casas J,Lazzari C,Insausti T,Launois P,Fouque F

    更新日期:2016-11-01 00:00:00

  • A checklist of arthropods associated with pig carrion and human corpses in southeastern brazil.

    abstract::Necrophagous insects, mainly Diptera and Coleoptera, are attracted to specific stages of carcass decomposition, in a process of faunistic succession. They are very important in estimating the postmortem interval, the time interval between the death and the discovery of the body. In studies done with pig carcasses expo...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000100023

    authors: Carvalho LM,Thyssen PJ,Linhares AX,Palhares FA

    更新日期:2000-01-01 00:00:00

  • [Morbidity of schistosomiasis mansoni in Brazil. Developmental study in an endemic area over a 10-year period].

    abstract::Four cross sectional studies on morbidity of schistosomiasis mansoni were carried out respectively in 1973, 1974, 1979 and 1983 in the Village of Capitão Andrade, District of Itanhomi, State of Minas Gerais, Brazil, through clinical and fecal examination of the population. In addition a comparison was made of the dina...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761984000400009

    authors: Coura JR,Conceição MJ,Pereira JB

    更新日期:1984-10-01 00:00:00

  • Reduced capacity of peritoneal exudate cells for oxidative killing of Schistosoma mansoni schistosomula.

    abstract::Peritoneal exudate cells from mice infected with Schistosoma mansoni (S-PEC) can kill schistosomula in vitro in the presence of immune serum. S-PEC produce a low level of respiratory burst, and schistosomula mortality in their presence is not reduced when exogenous antioxidants are added, suggesting that with S-PEC, o...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761987000800052

    authors: Smith JM,Mikoji GM,Prichard RK

    更新日期:1987-01-01 00:00:00

  • A comparative study of fat body morphology in five mosquito species.

    abstract::The insect fat body plays major roles in the intermediary metabolism, in the storage and transport of haemolymph compounds and in the innate immunity. Here, the overall structure of the fat body of five species of mosquitoes (Aedes albopictus, Aedes fluviatilis, Culex quinquefasciatus, Anopheles aquasalis and Anophele...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000600015

    authors: Martins GF,Serrão JE,Ramalho-Ortigão JM,Pimenta PF

    更新日期:2011-09-01 00:00:00

  • Frequency of human toxocariasis in Jos, Plateau State, Nigeria.

    abstract::The enzyme-linked immunosorbent assay (Elisa) was used to examine sera of 104 children and adults in Jos, Plateau State, Nigeria for anti-toxocaral antibodies, out of which 31 (29.8%) were reactive. The seropositive rates were 30.4% for adults, 29.6% for children, 34% for females and 25.9% for males. However, the diff...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/S0074-02762000000200002

    authors: Ajayi OO,Duhlinska DD,Agwale SM,Njoku M

    更新日期:2000-03-01 00:00:00

  • Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages.

    abstract::Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expressio...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760170105

    authors: Villela AD,Rodrigues-Junior VS,Pinto AFM,Falcão VCA,Sánchez-Quitian ZA,Eichler P,Bizarro CV,Basso LA,Santos DS

    更新日期:2017-11-01 00:00:00

  • Studies on the virulence and attenuation of Trypanosoma cruzi using immunodeficient animals.

    abstract::Tissue invasion and pathology by Trypanosoma cruzi result from an interaction between parasite virulence and host immunity. Successive in vivo generations of the parasite select populations with increasing ability to invade the host. Conversely, prolonged in vitro selection of the parasite produces attenuated sublines...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000700029

    authors: Basombrío MA,Segura MA,Gómez L,Padilla M

    更新日期:2000-01-01 00:00:00

  • Future trypanosomatid phylogenies: refined homologies, supertrees and networks.

    abstract::There has been good progress in inferring the evolutionary relationships within trypanosomes from DNA data as until relatively recently, many relationships have remained rather speculative. Ongoing molecular studies have provided data that have adequately shown Trypanosoma to be monophyletic and, rather surprisingly, ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000400014

    authors: Stothard J

    更新日期:2000-07-01 00:00:00

  • Schistosoma mansoni heat shock protein 70 elicits an early humoral immune response in S. mansoni infected baboons.

    abstract::A study was undertaken to search for DNA recombinant Schistosoma mansoni proteins responsible for eliciting an antibody response from the host at a very early phase after infection. A S. mansoni adult worm cDNA expression library was screened using pooled sera from baboons with four weeks of infection. Based on their ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000500022

    authors: Kanamura HY,Hancock K,Rodrigues V,Damian RT

    更新日期:2002-07-01 00:00:00

  • Integrated genetic epidemiology of infectious diseases: the Chagas model.

    abstract::Genetic typing of pathogenic agents and of vectors has known impressive developments in the last 10 years, thanks to the progresses of molecular biology, and to the contribution of the concepts of evolutionary genetics. Moreover, we know more and more on the genetic susceptibility of man to infectious diseases. I prop...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000500003

    authors: Tibayrenc M

    更新日期:1998-09-01 00:00:00

  • The vitellogenin gene family of Aedes aegypti.

    abstract::We have been interested in identifying genes that play a role in reproduction of the mosquito Aedes aegypti. Our interests are currently focused on the vitellogenin genes which in the mosquito are expressed only in the fat body in response to the insect steroid hormone, 20-hydroxyecdysone. Four of the five vitellogeni...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761987000700021

    authors: Hamblin MT,Marx JL,Wolfner MF,Hagedorn HH

    更新日期:1987-01-01 00:00:00

  • Diphtheria-neutralizing antibody levels in healthy adults from Rio de Janeiro, Brazil.

    abstract::In Brazil, until 2004, the immunization policy against diphtheria involved childhood vaccination with no official routine booster dose administered after 15 years of age. This study assessed functional antibody levels against diphtheria among blood donors. A total of 140 blood samples were collected, and diphtheria an...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762006000400019

    authors: Pimenta FP,Damasco PV,Cerbino Neto J,Lopes GS,Hirata R Jr,Milagres LG,Mattos-Guaraldi AL

    更新日期:2006-06-01 00:00:00

  • Analysis of E2 gene integrity in HPV16 and HPV58 viruses isolated from women with cervical pathology.

    abstract::Integration of human papillomavirus (HPV) DNA into human cells accompanied by the disruption of the viral genome has been described as a prerequisite for cancer development. This study aimed to investigate E2 gene integrity of HPV16 and HPV58 viruses isolated from infected women with cervical lesions. Forty-two HPV16-...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160269

    authors: González-Losa MD,Puerto-Solis M,Tenorio Ruiz J,Rosado-López AI,Hau-Aviles O,Ayora-Talavera G,Cisneros-Cutz I,Conde-Ferráez L

    更新日期:2016-12-01 00:00:00

  • The retained capacity of Lutzomyia longipalpis (Lutz & Neiva) to transmit Leishmania chagasi (Cunha & Chagas) after eight years (64 generations) in a closed laboratory colony.

    abstract::A closed Lutzomyia longipalpis colony, from Ceará has been used to transmit Leishmania chagasi isolated from a fox in Pará state. The last time this colony was successfully used in similar transmission experiments was eight years (64 generations) ago indicating that this colony of Lu. longipalpis has fully maintained ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761985000300010

    authors: Gonçalves MD,Ryan L,Lainson R,Shaw JJ

    更新日期:1985-07-01 00:00:00

  • Compatibility of Biomphalaria tenagophila with Schistosoma mansoni: a study of homologous plasma transference.

    abstract::This study aims to investigate the importance of the serum factors present in the plasma of resistant Biomphalaria tenagophila snails, when transferred to susceptible conspecific. Susceptible B. tenagophila (CF) received plasma from resistant B. tenagophila (Taim), and both were later infected with Schistosoma mansoni...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762006000100022

    authors: Coelho JR,Bezerra FS

    更新日期:2006-02-01 00:00:00

  • Drimanes from Drimys brasiliensis with leishmanicidal and antimalarial activity.

    abstract::This paper evaluates CHCl3 and CH3OH extracts of the stem bark, branches and leaves of Drimys brasiliensis and drimane sesquiterpenes isolated from the stem bark against strains of Leishmania amazonensis and Leishmania braziliensis promastigotes and Plasmodium falciparum trophozoites. All of the extracts and compounds...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276108022013002

    authors: Claudino VD,da Silva KC,Cechinel Filho V,Yunes RA,Delle Monache F,Giménez A,Salamanca E,Gutierrez-Yapu D,Malheiros A

    更新日期:2013-04-01 00:00:00

  • Ecology of cutaneous leishmaniasis in Sinai: linking parasites, vectors and hosts.

    abstract::Cutaneous leishmaniasis (CL) is a neglected clinical form of public health importance that is quite prevalent in the northern and eastern parts of Egypt. A comprehensive study over seven years (January 2005-December 2011) was conducted to track CL transmission with respect to both sandfly vectors and animal reservoirs...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276130426

    authors: Samy AM,Doha SA,Kenawy MA

    更新日期:2014-06-01 00:00:00

  • N-terminal amino acid sequences of the major outer membrane proteins from a Neisseria meningitidis group B strain isolated in Brazil.

    abstract::The four dominant outer membrane proteins (46, 38, 33 and 28 kDa) were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in a semi-purified preparation of vesicle membranes of a Neisseria meningitidis (N44/89, B:4:P1.15:P5.5,7) strain isolated in Brazil. The N-terminal amino acid sequenc...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761996000100020

    authors: De Simone SG,Soares SA,Souza AL,Danelli MG

    更新日期:1996-01-01 00:00:00

  • Chimpanzees and supporting models in the study of malaria pre-erythrocytic stages.

    abstract::Chimpanzees are being used in the study of immune response to Plasmodium falciparum malaria pre-erythrocytic stages (MPES). Responses induced by immunisation with recombinant/synthetic antigens and by irradiated sporozoites are being evaluated in a model system that is phylogenetically close to humans and that is amen...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761994000600023

    authors: Thomas AW,Slierendregt B,Mons B,Druilhe P

    更新日期:1994-01-01 00:00:00

  • Descriptions of Lutzomyia (Evandromyia) georgii n. sp. and a synopsis of the series infraspinosa (Diptera: Psychodidae).

    abstract::Lutzomyia georgii n. sp. and the female of L. tarapacaensis in the Series infraspinosa of the subgenus Evandromyia are described, from specimens collected in rainforest in the north of the State of Pará, Brazil. The new species was taken together with five other Evandromyia species including L. infraspinosa (sensu str...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000200017

    authors: Freitas RA,Barrett TV

    更新日期:2002-03-01 00:00:00

  • Prevalence and genotypes of GB virus C/hepatitis G virus among blood donors in Central Brazil.

    abstract::A survey was conducted in a blood donor population of Central Brazil aiming to investigate the prevalence of GB virus C (GBV-C)/hepatitis G virus (HGV) infection and also to analyze the virus genotypes distribution. A total of 241 voluntary blood donors were interviewed at the State Blood Bank in Goiânia, State of Goi...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000700005

    authors: Oliveira LA,Martins RM,Carneiro MA,Teles SA,Silva SA,Cardoso DD,Lampe E,Yoshida CF

    更新日期:2002-10-01 00:00:00

  • Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections.

    abstract::An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a pr...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760140457

    authors: Bentes GA,Lanzarini NM,Lima LR,Manso PP,da Silva Ados S,Mouta Junior Sda S,Guimarães JR,de Moraes MT,Pelajo-Machado M,Pinto MA

    更新日期:2015-06-01 00:00:00

  • Treatment of human acute schistosomiasis with oxamniquine induces an increase in interferon-gamma response to Schistosoma mansoni antigens.

    abstract::Patients with acute schistosomiasis were studied before and after oxamniquine treatment. They had been exposed to cercariae 5 to 9 weeks before, and presented compatible clinical manifestations, eosinophilia, and high levels of total IgE. Interferon-gamma (IFN-gamma) and interleukin-4 were measured by ELISA in whole b...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000002

    authors: de Souza JR,Morais CN,Aroucha ML,Miranda PJ,Barbosa CS,Domingues AL,Carvalho Júnior LB,Abath FG,Montenegro SM

    更新日期:2007-05-01 00:00:00

  • Bonafide, type-specific human papillomavirus persistence among HIV-positive pregnant women: predictive value for cytological abnormalities, a longitudinal cohort study.

    abstract::This study investigated the rate of human papillomavirus (HPV) persistence, associated risk factors, and predictors of cytological alteration outcomes in a cohort of human immunodeficiency virus-infected pregnant women over an 18-month period. HPV was typed through L1 gene sequencing in cervical smears collected durin...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760150393

    authors: Meyrelles AR,Siqueira JD,Santos PP,Hofer CB,Luiz RR,Seuánez HN,Almeida G,Soares MA,Soares EA,Machado ES

    更新日期:2016-02-01 00:00:00

  • Comparative descriptions of eggs from three species of Rhodnius (Hemiptera: Reduviidae: Triatominae).

    abstract::The authors describe and compare the morphological and ultrastructural characteristics of eggs from the three most recent described species of the genus Rhodnius Stål, 1859, which have not previously been studied. These species are Rhodnius colombiensis (Mejia, Galvão & Jurberg 1999), Rhodnius milesi (Carcavallo, Roch...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000700013

    authors: Santos CM,Jurberg J,Galvão C,Rosa JA,Júnior WC,Barata JM,Obara MT

    更新日期:2009-11-01 00:00:00

  • Determinants of human immunodeficiency virus (HIV) prevalence in homosexual and bisexual men screened for admission to a cohort study of HIV negatives in Belo Horizonte, Brazil: Project Horizonte.

    abstract::Project Horizonte, an open cohort of homosexual and bisexual human immunodeficiency virus (HIV-1) negative men, is a component of the AIDS Vaccine Program, in Belo Horizonte, Minas Gerais, Brazil. The objective of this study was to compare volunteers testing HIV positive at cohort entry with a sample of those who test...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000300006

    authors: Carneiro M,Cardoso FA,Greco M,Oliveira E,Andrade J,Greco DB,Antunes CM

    更新日期:2003-04-01 00:00:00

  • [Sparganosis in some Brazilian vertebrates. Difficulties in the identification of Luheella (Spirometra) species].

    abstract::Some species of Amphibia and Reptilia are listed as new hosts of spargana, from material deposited in the Helminthological Collection of Oswaldo Cruz Institute. It is discussed the difficulties in identifying the larvae (Sparganum) and also the identification of adults of Luheella species from South America. The histo...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761992000500040

    authors: Rego AA,Schäffer GV

    更新日期:1992-01-01 00:00:00

  • Human toxocariasis: a seroepidemiological survey in schoolchildren of Sorocaba, Brazil.

    abstract::A seroepidemiological survey for toxocariasis, among 180 schoolchildren of the public schools of Sorocaba City, state of São Paulo, Brazil, was carried out from August 2000 to July 2001. ELISA test was performed using excretory and secretory antigens for the detection of IgG anti-Toxocara antibodies. Information regar...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:/S0074-02762004000600002

    authors: Coelho LM,Silva MV,Dini CY,Giacon Neto AA,Novo NF,Silveira EP

    更新日期:2004-10-01 00:00:00