Schistosoma mansoni heat shock protein 70 elicits an early humoral immune response in S. mansoni infected baboons.

Abstract:

:A study was undertaken to search for DNA recombinant Schistosoma mansoni proteins responsible for eliciting an antibody response from the host at a very early phase after infection. A S. mansoni adult worm cDNA expression library was screened using pooled sera from baboons with four weeks of infection. Based on their specific reactivity with the S. mansoni infected sera and no reactivity when tested against the pre-infection sera from the same baboons, four clones were selected for further studies. Sequence analysis revealed that they were homologous to the S. mansoni heat shock protein 70 (hsp70). The insert sizes of the four selected clones varied from 1150 to 2006 bp. The preliminary characterization for antibody reactivity against a panel of baboon sera showed that the longest clone was the most reactive, eight out of eight acute and three out of four chronic sera reacting positively to this clone. The shortest clone was the least reactive. Our results suggest that the S. mansoni hsp70 elicits an early and strong antibody response in baboons and that antibodies to this protein can be detected in chronically infected animals. Therefore S. mansoni hsp70 may be a valid target for immunodiagnosis. However further studies are needed to identify the portion of the hsp70 that best fits the requirements for a valuable diagnostic antigen.

journal_name

Mem Inst Oswaldo Cruz

authors

Kanamura HY,Hancock K,Rodrigues V,Damian RT

doi

10.1590/s0074-02762002000500022

subject

Has Abstract

pub_date

2002-07-01 00:00:00

pages

711-6

issue

5

eissn

0074-0276

issn

1678-8060

pii

S0074-02762002000500022

journal_volume

97

pub_type

杂志文章
  • Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni.

    abstract::Studies based on shell or reproductive organ morphology and genetic considerations suggest extensive intraspecific variation in Biomphalaria snails. The high variability at the morphological and genetic levels, as well as the small size of some specimens and similarities between species complicate the correct identifi...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000700014

    authors: Vidigal TH,Dias Neto E,Spatz L,Nunes DN,Pires ER,Simpson AJ,Carvalho OS

    更新日期:1998-01-01 00:00:00

  • Description of Warileya lumbrerasi n. sp. (Diptera:Psychodidae) from Peru.

    abstract::Warileya lumbrerasi n. sp. is described from the northern Peruvian Andes. This species was collected inside houses, and is the seventh species described within the genus Warileya Hertig, 1948. ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761996000600010

    authors: Ogusuku E,Perez JE,Davies CR,Villaseca P

    更新日期:1996-11-01 00:00:00

  • Report of Didymocystis wedli Ariola, 1902 (Digenea; Didymozoidae) from Thunnus albacares in Brazil.

    abstract::Didymocystis wedli a parasite from the gills of Thunnus albacares from the coast of the State of Rio de Janeiro, is described by use of light and scanning electron microscopy. This is the first report of this species in Brazil and South America. New data are presented on the surface topography as demonstrated by scann...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000700011

    authors: Kohn A,Santos A,Baptista-Farias MF

    更新日期:2001-10-01 00:00:00

  • Control of Mycobacterium fortuitum and Mycobacterium intracellulare infections with respect to distinct granuloma formations in livers of BALB/c mice.

    abstract::Mycobacterium fortuitum is a rapidly growing nontuberculous Mycobacterium that can cause a range of diseases in humans. Complications from M. fortuitum infection have been associated with numerous surgical procedures. A protective immune response against pathogenic mycobacterial infections is dependent on the granulom...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000500007

    authors: Silva TR,Petersen AL,Santos Tde A,Almeida TF,Freitas LA,Veras PS

    更新日期:2010-08-01 00:00:00

  • TcruziDB, an integrated database, and the WWW information server for the Trypanosoma cruzi genome project.

    abstract::Data analysis, presentation and distribution is of utmost importance to a genome project. A public domain software, ACeDB, has been chosen as the common basis for parasite genome databases, and a first release of TcruziDB, the Trypanosoma cruzi genome database, is available by ftp from ftp://iris.dbbm.fiocruz.br/pub/g...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761997000600015

    authors: Degrave W,de Miranda AB,Amorim A,Brandão A,Aslett M,Vandeyar M

    更新日期:1997-11-01 00:00:00

  • Mapping the global geographic potential of Zika virus spread.

    abstract::The Americas are presently experiencing the most serious known outbreak of Zika virus (ZIKV). Here, we present a novel set of analyses using environmental characteristics, vector mosquito distributions, and socioeconomic risk factors to develop the first map to detail global ZIKV transmission risk in multiple dimensio...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160149

    authors: Samy AM,Thomas SM,Wahed AA,Cohoon KP,Peterson AT

    更新日期:2016-09-01 00:00:00

  • Non-immunologic methods of diagnosis of babesiosis.

    abstract::The diagnosis of tick-borne diseases such as babesiosis still depends on observing the parasite in the infected erythrocyte. Microscopic observation is tedious and often problematic in both early and carrier infections. Better diagnostic methods are needed to prevent clinical disease, especially when susceptible cattl...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761992000700032

    authors: Wagner G,Cruz D,Holman P,Waghela S,Perrone J,Shompole S,Rurangirwa F

    更新日期:1992-01-01 00:00:00

  • The fumigant and repellent activity of aliphatic lactones against Pediculus humanus capitis (Anoplura: Pediculidae).

    abstract::New alternative insecticides are necessary for the chemical control of head lice. In this study the fumigant knockdown time 50% (KT50) and repellency index (RI) of three aliphatic lactones was compared with two essential oils and DDVP, against permethrin-resistance Pediculus humanus capitis from Argentina. In the fumi...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762006000100010

    authors: Toloza AC,Zygadlo J,Mougabure-Cueto G,Zerba E,Faillaci S,Picollo MI

    更新日期:2006-02-01 00:00:00

  • Diagnostic challenges of single plaque-like lesion paucibacillary leprosy.

    abstract::The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276140212

    authors: Barbieri RR,Sales AM,Illarramendi X,Moraes MO,Nery JA,Moreira SJ,Sarno EN,Machado Ade M,Bozza FA

    更新日期:2014-11-01 00:00:00

  • Using a single tablet daily to treat latent tuberculosis infection in Brazil: bioequivalence of two different isoniazid formulations (300 mg and 100 mg) demonstrated by a sensitive and rapid high-performance liquid chromatography-tandem mass spectrometry

    abstract::The recommended treatment for latent tuberculosis (TB) infection in adults is a daily dose of isoniazid (INH) 300 mg for six months. In Brazil, INH was formulated as 100 mg tablets. The treatment duration and the high pill burden compromised patient adherence to the treatment. The Brazilian National Programme for Tube...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,随机对照试验

    doi:10.1590/0074-02760140458

    authors: Daher A,Pitta L,Santos T,Barreira D,Pinto D

    更新日期:2015-06-01 00:00:00

  • Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil.

    abstract:BACKGROUND:Enterocytozoon bieneusi are the most common microsporidia associated with different clinical manifestations such as diarrhoea, respiratory tract inflammation and acalculous cholecystitis, especially in immunocompromised patients. Infection usually occurs by ingestion of food and water contaminated with spore...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160435

    authors: Yamashiro S,Fiuza VRDS,Teixeira ÂTLS,Branco N,Levy CE,Castro ICVS,Franco RMB

    更新日期:2017-06-01 00:00:00

  • Synonymy between Lymnaea bogotensis Pilsbry, 1935 and Lymnaea cousini Jousseaume, 1887 (Gastropoda: Lymnaeidae).

    abstract::The lymneid snail Lymnaea bogotensis Pilsbry 1935 is synonymized with L. cousini Jousseaume 1887, based on morphological comparisons of the reproductive systems. The shell, renal tube, and reproductive system are described and illustrated from specimens collected in the type locality and the municipality of Paipa, Col...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762006000700015

    authors: Velásquez LE

    更新日期:2006-11-01 00:00:00

  • Reliability of current estimates of schistosomiasis prevalence in the Rainforest Zone of the state of Pernambuco, Northeastern Brazil.

    abstract::The Program for Schistosomiasis Control within the Unified Health System (PCE-SUS) was implemented by 1999 in the Rainforest Zone or "Zona da Mata" of Pernambuco (ZMP) aiming to carry out biennial stool surveys of whole populations through municipal health organs followed by treatment of the positives through the loca...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762006000900011

    authors: Favre TC,Ximenes RA,Galvão AF,Pereira AP,Wanderlei TN,Barbosa CS,Pieri OS

    更新日期:2006-09-01 00:00:00

  • In vitro and in vivo anti-Trypanosoma cruzi activity of a novel nitro-derivative.

    abstract::Nitroarylidenemalononitriles and their cyanoacetamide derivatives with remarkable anti-epimastigote properties, were synthesized attempting to obtain new 3,5-diamino-4-(5'-nitroarylidene)-4H-thiadiazine 1,1-dioxide derivatives, which in previous reports had shown anti-Trypanosoma cruzi activity. Tests to evaluate the ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000400019

    authors: Muelas-Serrano S,Le-Senne A,Fernandez-Portillo C,Nogal JJ,Ochoa C,Gomez-Barrio A

    更新日期:2002-06-01 00:00:00

  • Draft genome of the Leptospira interrogans strains, Acegua, RCA, Prea, and Capivara, obtained from wildlife maintenance hosts and infected domestic animals.

    abstract::In the present paper, we announce new draft genomes of four Leptospira interrogans strains named Acegua, RCA, Prea, and Capivara. These strains were isolated in the state of Rio Grande do Sul, Brazil, from cattle, dog, Brazilian guinea pig, and capybara, respectively. ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160010

    authors: Kremer FS,Eslabão MR,Jorge S,Oliveira NR,Labonde J,Santos MN,Monte LG,Grassmann AA,Cunha CE,Forster KM,Moreno LZ,Moreno AM,Campos VF,McBride AJ,Pinto LS,Dellagostin OA

    更新日期:2016-04-01 00:00:00

  • Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region.

    abstract::We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000600016

    authors: Tang TH,López-Vélez R,Lanza M,Shelley AJ,Rubio JM,Luz SL

    更新日期:2010-09-01 00:00:00

  • Taenia solium metacestode immunodominant peptides recognized by IgG antibodies in cerebrospinal fluid and serum paired samples from patients with active and inactive neurocysticercosis.

    abstract::The aim of this study was to test if serological distinction between patients with active and inactive neurocysticercosis (NCC), could be accomplished by the recognition of immunodominant peptides in total saline antigenic extract of Taenia solium metacestodes by IgG antibody in cerebrospinal fluid (CSF) and serum pai...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000084

    authors: Barcelos IS,Moura LP,Costa VP,Ferreira MS,Costa-Cruz JM

    更新日期:2007-09-01 00:00:00

  • Ultrastructure of endogenous stages of Eimeria ninakohlyakimovae Yakimoff & Rastegaieff, 1930 Emend. Levine, 1961 in experimentally infected goat.

    abstract::The ultrastructure of endogenous stages of Eimeria ninakohlyakimovae was observed in epithelial cells of cecum and colon crypts from a goat experimentally infected with 2.0 x 10(5) oocysts/kg. The secondary meronts developed above the nucleus of the host cell. The nucleus first divides and merozoites then form on the ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761997000400017

    authors: Vieira LS,Lima JD,Ribeiro MF,Bozzi IA,Camargos ER

    更新日期:1997-07-01 00:00:00

  • Plants against snake-bites.

    abstract::Small molecular weight compounds from Mandevilla velutina and from Eclipta prostata were found to be active against snakebite. ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761991000600044

    authors: Mors WB

    更新日期:1991-01-01 00:00:00

  • Self-fertilization in the freshwater snails Helisoma duryi and Helisoma trivolvis.

    abstract::Fifty specimens of five strains (10 per strain) of Helisoma duryi from Lima (Peru), St. Croix (Virgin Islands), Formosa (Brazil), Cartago (Costa Rica) and St. Vincent (Lesser Antilles), reared in isolation for about 150 days, laid 103 eggs. The numbers of eggs laid by the 10 specimens of each strain were respectively ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761988000400002

    authors: Paraense WL,Corrêa LR

    更新日期:1988-10-01 00:00:00

  • The study of insect blood-feeding behaviour. 1: Feeding equipment, physical and endogenous factors, dose effect analysis, and diet destination.

    abstract::Experimental techniques that we have found useful during our studies of insect blood-feeding behaviour are reviewed. Some of the principal findings resulting from these techniques are discussed. Where directly applicable, the work of others is included, but no complete review of the subject has been attempted. ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761987000700005

    authors: Friend WG,Smith JJ

    更新日期:1987-01-01 00:00:00

  • Zoonotic parasites associated with felines from the Patagonian Holocene.

    abstract::Feline coprolites were examined for parasites with the aim of studying ancient infections that occurred in the Patagonian region during the Holocene period. Eggs compatible to Trichuris sp., Calodium sp., Eucoleus sp., Nematodirus sp., Oesophagostomum sp. (Nematoda), Monoecocestus sp. (Cestoda) and Eimeria macusaniens...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000800016

    authors: Fugassa MH,Beltrame MO,Bayer MS,Sardella NH

    更新日期:2009-12-01 00:00:00

  • First genotyping of Giardia lamblia from human and animal feces in Argentina, South America.

    abstract::The purpose of this study was to investigate the genotypes of Giardia lamblia from human and animal feces and their epidemiological and clinical characteristics in Argentina, South America. Seventy isolates, 60 from humans (adults and children), eight from dogs and two from cows were processed by polymerase chain reac...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762008000100015

    authors: Minvielle MC,Molina NB,Polverino D,Basualdo JA

    更新日期:2008-02-01 00:00:00

  • Some coccidial parasites of the lizard Amphisbaena alba (Reptilia: Amphisbaenia: Amphisbaenidae).

    abstract::Five parasites are described in the lizard Amphisbaena alba (Amphisbaenidae) from the state of Par , North Brazil. Mature oocysts of Choleoeimeria amphisbaenae n. sp., are passed already mature in the faeces. They are ellipsoidal-cylindrical, average 33.7 x 22.8 m and are devoid of micropyle, oocyst residuum or polar...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000700012

    authors: Lainson R

    更新日期:2003-10-01 00:00:00

  • Experimental murine schistosomiasis mansoni: establishment of the chronic phase of the disease.

    abstract::After the acute hyperergic phase of schistosomal infection, the chronic phase of the disease corresponds to the establishment of a relative equilibrium between the host and the parasite. This involves: (1) A shift from the predominance of the TH2 response observed in the acute phase, to the predominance of the TH1 res...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761992000800026

    authors: Borojevic R

    更新日期:1992-01-01 00:00:00

  • Human immunodeficiency virus type 1 neutralization by plasma from B or F genotype infected individuals.

    abstract::Anti-human immunodeficiency virus type 1 (HIV-1) "binding antibodies" (antibodies capable of binding to synthetic peptides or proteins) occur throughout HIV-1 infection, are high-titered and highly cross-reactive, as confirmed in this study by analyzing plasma from B and F genotype HIV-1 infected individuals. Plasma f...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762005000100016

    authors: Bongertz V,Teixeira SL,Grinztejn B,Pilotto JH,Veloso VG,Morgado MG,Bastos FI,Ouverney EP

    更新日期:2005-02-01 00:00:00

  • Changing the epidemiology of carbapenem-resistant Pseudomonas aeruginosa in a Brazilian teaching hospital: the replacement of São Paulo metallo-β-lactamase-producing isolates.

    abstract::In Brazil, carbapenem-resistant Pseudomonas aeruginosa isolates are closely related to the São Paulo metallo-β-lactamase (SPM) Brazilian clone. In this study, imipenem-resistant isolates were divided in two sets, 2002/2003 and 2008/2009, analysed by pulsed field gel electrophoresis and tested for the Ambler class B me...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000300019

    authors: Cavalcanti FL,Almeida AC,Vilela MA,Morais MM,Morais Junior MA

    更新日期:2012-05-01 00:00:00

  • Localization of Brugia malayi (sub-periodic) adults in different organs of Mastomys coucha and its influence on microfilaraemia and host antibody response.

    abstract::Lymphatic filariasis caused by nematode parasites Wuchereria bancrofti or Brugia malayi is a spectral disease and produces wide range of immune responses and varying levels of microfilaraemia in infected individuals. The relationship between the immune response of host and the developmental stage of the parasite as we...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762006000300007

    authors: Athisaya Mary K,Hoti S,Paily K

    更新日期:2006-05-01 00:00:00

  • The antimicrobial activity of lapachol and its thiosemicarbazone and semicarbazone derivatives.

    abstract::Lapachol was chemically modified to obtain its thiosemicarbazone and semicarbazone derivatives. These compounds were tested for antimicrobial activity against several bacteria and fungi by the broth microdilution method. The thiosemicarbazone and semicarbazone derivatives of lapachol exhibited antimicrobial activity a...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/S0074-02762013000300013

    authors: Souza MA,Johann S,Lima LA,Campos FF,Mendes IC,Beraldo H,Souza-Fagundes EM,Cisalpino PS,Rosa CA,Alves TM,de Sá NP,Zani CL

    更新日期:2013-05-01 00:00:00

  • Related factors to human toxocariasis in a rural community of Argentina.

    abstract::The objective of this study was to evaluate the relationship between toxocariasis frequency and demographic, environmental, sanitary variables, eosinophylia, and other intestinal parasites in a rural population of Argentina. Serological examination of 100 individuals was carried out by using ELISA technique for the de...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762006000400009

    authors: Chiodo P,Basualdo J,Ciarmela L,Pezzani B,Apezteguía M,Minvielle M

    更新日期:2006-06-01 00:00:00