Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni.

Abstract:

:Studies based on shell or reproductive organ morphology and genetic considerations suggest extensive intraspecific variation in Biomphalaria snails. The high variability at the morphological and genetic levels, as well as the small size of some specimens and similarities between species complicate the correct identification of these snails. Here we review our work using methods based on polymerase chain reaction (PCR) amplification for analysis of genetic variation and identification of Biomphalaria snails from Brazil, Argentina, Uruguay and Paraguay. Arbitrarily primed-PCR revealed that the genome of B. glabrata exhibits a remarkable degree of intraspecific polymorphism. Low stringency-PCR using primers for 18S rRNA permitted the identification of B. glabrata, B. tenagophila and B. occidentalis. The study of individuals obtained from geographically distinct populations exhibits significant intraspecific DNA polymorphism, however, specimens from the same species, exhibit some species specific LSPs. We also showed that PCR-restriction fragment of length polymorphism of the internal transcribed spacer region of Biomphalaria rDNA, using Ddel permits the differentiation of the three intermediate hosts of Schistosoma mansoni. the molecular biological techniques used in our studies are very useful for the generation of new knowledge concerning the systematics and population genetics of Biomphalaria snails.

journal_name

Mem Inst Oswaldo Cruz

authors

Vidigal TH,Dias Neto E,Spatz L,Nunes DN,Pires ER,Simpson AJ,Carvalho OS

doi

10.1590/s0074-02761998000700014

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

103-10

eissn

0074-0276

issn

1678-8060

pii

S0074-02761998000700014

journal_volume

93 Suppl 1

pub_type

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