Genetic an antigenic analysis of adenovirus type 3 strains showing intermediate behavior in standard seroneutralization test.

Abstract:

:During an epidemiological survey of acute respiratory infection in Rio de Janeiro, among 208 adenovirus isolates, we found two strains that we were not able, by a standard neutralization procedure, to distinguish between type 3 or 7. However, DNA restriction pattern for the two strains with different enzymes were analyzed and showed a typical Ad3h profile. Using a cross-neutralization test in which both Ad3p and Ad7p antisera were used in different concentration against 100 TCID50 of each adenovirus standard and both isolates, we were able to confirm that the two isolates belong to serotype 3. An hemagglutination inhibition test also corroborated the identification of both strains as adenovirus type 3. Comparing Ad3h and Ad3p genome, we observed 16 different restriction enzyme sites, three of which were located in genomic regions encoding polypeptides involved in neutralization sites.

journal_name

Mem Inst Oswaldo Cruz

authors

Moraes MT,da Silva M,Leite JP,Nascimento JP

doi

10.1590/s0074-02761998000200019

subject

Has Abstract

pub_date

1998-03-01 00:00:00

pages

231-5

issue

2

eissn

0074-0276

issn

1678-8060

pii

S0074-02761998000200019

journal_volume

93

pub_type

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