Abstract:
:Cells die through a programmed process or accidental death, know as apoptosis or necrosis, respectively. Bothrops jararaca is a snake whose venom inhibits the growth of Trypanosoma cruzi epimastigote forms causing mitochondrion swelling and cell death. The aim of the present work was to determine the type of death induced in epimastigotes of T. cruzi by this venom. Parasite growth was inhibited after venom treatment, and 50% growth inhibition was obtained with 10 microg/ml. Ultrastructural observations confirmed mitochondrion swelling and kinetoplast disorganization. Furthermore, cytoplasmic condensation, loss of mitochondrion membrane potential, time-dependent increase in phosphatidylserine exposure at the outer leaflet plasma membrane followed by permeabilization, activation of caspase like protein and DNA fragmentation were observed in epimastigotes throughout a 24 h period of venom treatment. Taken together, these results indicate that the stress induced in epimastigote by this venom, triggers a programmed cell death process, similar to metazoan apoptosis, which leads to parasite death.
journal_name
Mem Inst Oswaldo Cruzjournal_title
Memorias do Instituto Oswaldo Cruzauthors
Deolindo P,Teixeira-Ferreira AS,Melo EJ,Arnholdt AC,Souza Wd,Alves EW,DaMatta RAdoi
10.1590/s0074-02762005000100006subject
Has Abstractpub_date
2005-02-01 00:00:00pages
33-8issue
1eissn
0074-0276issn
1678-8060pii
S0074-02762005000100006journal_volume
100pub_type
杂志文章abstract::In a recent issue of Memórias do Instituto Oswaldo Cruz, published in Rio de Janeiro in February 2014 (109: 87-92), Adami et al. have published a survey reporting Mansonella parasite prevalence in the Amazon Region. This report makes a useful contribution to the existing knowledge of filarial parasite distribution wit...
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journal_title:Memorias do Instituto Oswaldo Cruz
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journal_title:Memorias do Instituto Oswaldo Cruz
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journal_title:Memorias do Instituto Oswaldo Cruz
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pub_type: 临床试验,杂志文章,随机对照试验
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