Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom.

Abstract:

:Cells die through a programmed process or accidental death, know as apoptosis or necrosis, respectively. Bothrops jararaca is a snake whose venom inhibits the growth of Trypanosoma cruzi epimastigote forms causing mitochondrion swelling and cell death. The aim of the present work was to determine the type of death induced in epimastigotes of T. cruzi by this venom. Parasite growth was inhibited after venom treatment, and 50% growth inhibition was obtained with 10 microg/ml. Ultrastructural observations confirmed mitochondrion swelling and kinetoplast disorganization. Furthermore, cytoplasmic condensation, loss of mitochondrion membrane potential, time-dependent increase in phosphatidylserine exposure at the outer leaflet plasma membrane followed by permeabilization, activation of caspase like protein and DNA fragmentation were observed in epimastigotes throughout a 24 h period of venom treatment. Taken together, these results indicate that the stress induced in epimastigote by this venom, triggers a programmed cell death process, similar to metazoan apoptosis, which leads to parasite death.

journal_name

Mem Inst Oswaldo Cruz

authors

Deolindo P,Teixeira-Ferreira AS,Melo EJ,Arnholdt AC,Souza Wd,Alves EW,DaMatta RA

doi

10.1590/s0074-02762005000100006

subject

Has Abstract

pub_date

2005-02-01 00:00:00

pages

33-8

issue

1

eissn

0074-0276

issn

1678-8060

pii

S0074-02762005000100006

journal_volume

100

pub_type

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