RS virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay.

Abstract:

:Two techniques for rapid diagnosis, immunofluorescence (IFAT) and enzyme immunoassay (EIA), have been compared with virus isolation in tissue culture for the detection of respiratory syncytial virus (RSV) in specimens of nasopharyngeal secretions. The specimens were obtained from children under five years of age suffering from acute respiratory illness, during a period of six months from January to June 1982. Of 471 specimens examined 54 (11.5%) were positive by virus isolation and 180 (38.2%) were positive by immunofluorescence. The bacterial contamination of inoculated tissue cultures unfortunately prevented the isolation of virus from many samples. Specimens from 216 children were tested to compare enzyme immunoassay and immunofluorescence. Of these 60 (27%) were positive by EIA and 121 (56%) were positive by IFAT. Our results suggest that the EIA technique although highly specific is rather insensitive. This may be because by the time these tests were done the original nasopharyngeal secretions were considerably diluted and contained more mucus fragments than the cell suspension used for IFAT. Of the three techniques, IFAT gives the best results although EIA may be useful where IFAT is not possible.

journal_name

Mem Inst Oswaldo Cruz

authors

Siqueira MM,Ferreira V,Nascimento JP

doi

10.1590/s0074-02761986000200013

subject

Has Abstract

pub_date

1986-04-01 00:00:00

pages

225-32

issue

2

eissn

0074-0276

issn

1678-8060

pii

S0074-02761986000200013

journal_volume

81

pub_type

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