Abstract:
:A comparative study was undertaken on the immunogenic properties of 63kDa glycoproteins obtained from five different strains/species of Leishmania and assessed in C57BL/10 mice. The humoral immune response was assessed by ELISA against the five different antigens of the immunized animals. The cellular immune response was derived from Leishmania. The response was found to be species-specific in all of determined by means of the cytokine profiles secreted by the spleen cells of immunized animals. The presence of gamma-IFN and IL-2, and the absence of IL-4 in the supernatants of cells stimulated by L. amazonensis antigen established that the cellular response is of Th1 type. The five glycoproteins tested were equally effective in protecting C57BL/10 mice against challenge by L. amazonensis. About 50% of the immunized animals were protected for six months.
journal_name
Mem Inst Oswaldo Cruzjournal_title
Memorias do Instituto Oswaldo Cruzauthors
Dabés Guimarães TM,de Toledo VD,da Costa CA,da Costa RT,Genaro O,Williams P,Mayrink Wdoi
10.1590/s0074-02761996000100010subject
Has Abstractpub_date
1996-01-01 00:00:00pages
63-70issue
1eissn
0074-0276issn
1678-8060pii
S0074-02761996000100010journal_volume
91pub_type
杂志文章abstract::The aims of this study were to characterise the ground-level larval habitats of the mosquito Culex quinquefasciatus, to determine the relationships between habitat characteristics and larval abundance and to examine seasonal larval-stage variations in Córdoba city. Every two weeks for two years, 15 larval habitats (na...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-0276108062013014
更新日期:2013-09-01 00:00:00
abstract::A ribosome association factor (AF) was isolated from the yeast Sacchharomyces cerevisiae. Partial amino acid sequence of AF was determined from its fragment of 25 kDa isolated by treating AF with 2-(2-nitrophenylsulfenyl)-3-methyl-3'-Bromoindolenine (BNPS-skatole). This sequence has a 86% identity to the product of th...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762004000700012
更新日期:2004-11-01 00:00:00
abstract::The mechanisms that facilitate the adaptation of Trypanosoma cruzi to two distinct hosts, insect and vertebrate, are poorly understood, in part due to the limited ability to perform gene disruption studies by homologous recombination. This report describes a developmentally-defective phenotype that resulted from integ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762007000100019
更新日期:2007-02-01 00:00:00
abstract::The epidemiological features of rotavirus A (RVA) infection differ between children from developing and developed countries which could result in differences in vaccine efficacy around the world. To evaluate the impact of Rotarix™ on RVA prevalence, we monitored RVA genotypes circulating in Goiânia by monitoring virus...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762011000400018
更新日期:2011-06-01 00:00:00
abstract::A study was undertaken to search for DNA recombinant Schistosoma mansoni proteins responsible for eliciting an antibody response from the host at a very early phase after infection. A S. mansoni adult worm cDNA expression library was screened using pooled sera from baboons with four weeks of infection. Based on their ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762002000500022
更新日期:2002-07-01 00:00:00
abstract::The authors investigated the distribution of lectin receptors on Trypanosoma cruzi blood forms collected from mice inoculated with, respectively, the drug-resistant and drug-sensitive strains VL-10 and CL, and treated with the two standard active nitroheterocyclic compounds nifurtimox and benznidazole used for treatme...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761991000300003
更新日期:1991-07-01 00:00:00
abstract::Feline coprolites were examined for parasites with the aim of studying ancient infections that occurred in the Patagonian region during the Holocene period. Eggs compatible to Trichuris sp., Calodium sp., Eucoleus sp., Nematodirus sp., Oesophagostomum sp. (Nematoda), Monoecocestus sp. (Cestoda) and Eimeria macusaniens...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762009000800016
更新日期:2009-12-01 00:00:00
abstract::Positive Montenegro's skin test is a delayed type hypersensitivity reaction widely used as indicative of previous infection with Leishmania in both humans and dogs. Montenegro's antigen consists of a crude Leishmania antigen solution, usually containing thimerosal as preserving agent. In this work it is shown that a l...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762001000500017
更新日期:2001-07-01 00:00:00
abstract::Triatoma nitida was found in 14 (0.4%) out of 3,726 houses located in six departments across Guatemala, which were surveyed from 1994 to 1998 by the man-hour collection method. Compared to previous information, the distribution of T. nitida in Guatemala has increased from five to nine departments; the species is prese...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762003000100006
更新日期:2003-01-01 00:00:00
abstract::A one year-long study (March 1979-March 1980) was carried out at San Esteban, an endemic focus of cutaneous leishmaniasis in Northern Venezuela, with the aim of observing the seasonal fluctuation of the local phlebotomine sandflies species. The influence of climatic factors (temperature, relative humidity and rainfall...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761987000200003
更新日期:1987-04-01 00:00:00
abstract::A polysaccharide-rich fraction (ATF) of medicinal mushroom Agaricus brasiliensis was evaluated on the candidacidal activity, H2O2 and nitric oxide (NO) production, and expression of mannose receptors by murine peritoneal macrophages. Mice received three intraperitoneal (i.p.) injections of ATF and after 48 h their per...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762008005000011
更新日期:2008-05-01 00:00:00
abstract::Molecular evidence showed 46.2% of Trypanosoma cruzi infection in Mepraia spinolai insects from North-Central Chile, which is significantly higher than previous reports of up to 26% by microscopic observation. Our results show similar infection levels among nymphal stages, ranging from 38.3 to 54.1%, indicating that y...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762005000300003
更新日期:2005-05-01 00:00:00
abstract::The natural lignans veraguensin and grandisin have been reported to be active against Trypanosoma cruzi bloodstream forms. Aiming at the total synthesis of these and related compounds, we prepared three 2-arylfurans and eight 2,5-diarylfurans. They were evaluated for their potential as T. cruzi trypanothione reductase...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762006000200009
更新日期:2006-03-01 00:00:00
abstract::The objective of this work was to characterize, and compare different morphological types of hemocytes of Rhodnius prolixus, Rhodnius robustus, Rhodnius neglectus, Triatoma infestans, Panstrongylus megistus, and Dipetalogaster maximus. This information provides the basis for studying the cellular immune systems of the...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761991000100002
更新日期:1991-01-01 00:00:00
abstract::Schistosoma mansoni is one of the three main causative agents of human schistosomiasis, a major health problem with a vast socio-economic impact. Recent advances in the proteomic analysis of schistosomes have revealed that peptidases are the main virulence factors involved in the pathogenesis of this disease. In this ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762011000700013
更新日期:2011-11-01 00:00:00
abstract::Neisseria meningitidis retains its ability to cause endemic and hiperendemic disease in human population living in any environment, as well as localized outbreaks and massive epidemics in civilians and military personnel. In Rio de Janeiro it has been reported in the 1990s as prolonged outbreak of serogroup B and at l...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762007005000104
更新日期:2007-09-01 00:00:00
abstract::The dual function of eosinophils has been evidenced in protective immunity against parasites as well as in pathological manifestations during allergic disorders. We have demonstrated that a new class of IgE receptors, Fc epsilon RII/CD23, was involved in the functional duality of eosinophils and other proinflammatory ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02761997000800003
更新日期:1997-01-01 00:00:00
abstract::Muscina stabulans, M. domestica, Chrysomya putoria, C. megacephala and Stomoxys calcitrans were the most abundant muscoid flies captured in a poultry facility in southeastern Brazil. We examined the gonadotrophic profiles of the females caught at different sites and different times and found that Mu. stabulans and M. ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762000000200022
更新日期:2000-03-01 00:00:00
abstract::The biochemical and serological characteristics, virulence properties, and genetic relatedness of Shiga toxin-producing Escherichia coli (STEC) strains isolated in S o Paulo, from April 1989 through March 1990, were determined. This is also the first report on clinic findings of human STEC infections in Brazil. The on...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762002000800003
更新日期:2002-12-01 00:00:00
abstract::Genetic typing of pathogenic agents and of vectors has known impressive developments in the last 10 years, thanks to the progresses of molecular biology, and to the contribution of the concepts of evolutionary genetics. Moreover, we know more and more on the genetic susceptibility of man to infectious diseases. I prop...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761998000500003
更新日期:1998-09-01 00:00:00
abstract::The thymus is a central lymphoid organ, in which T cell precursors differentiate and generate most of the so-called T cell repertoire. Along with a variety of acute infectious diseases, we and others determined important changes in both microenvironmental and lymphoid compartments of the organ. For example, one major ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02761992000900010
更新日期:1992-01-01 00:00:00
abstract::This study sought the characterization of rotaviruses in a trial with a tetravalent rhesus-human rotavirus vaccine in Belém, Brazil in children who received three doses of vaccine or placebo in the 1st, 3rd and 5th months of life. Rotavirus electropherotypes, subgroups, G serotypes, G, [P] and [P], G genotypes were de...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1590/s0074-02762002000100020
更新日期:2002-01-01 00:00:00
abstract::Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly contagious infection that may break the healthcare system of several countries. Here, we aimed at presenting a critical view of ongoing drug repurposing efforts for COVID-19 as well as discussing ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760200254
更新日期:2020-10-02 00:00:00
abstract::In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with sever...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762012000100012
更新日期:2012-02-01 00:00:00
abstract::The Program for Schistosomiasis Control within the Unified Health System (PCE-SUS) was implemented by 1999 in the Rainforest Zone or "Zona da Mata" of Pernambuco (ZMP) aiming to carry out biennial stool surveys of whole populations through municipal health organs followed by treatment of the positives through the loca...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762006000900011
更新日期:2006-09-01 00:00:00
abstract::Trypanosoma cruzi acute infections often go unperceived, but one third of chronically infected individuals die of Chagas disease, showing diverse manifestations affecting the heart, intestines, and nervous systems. A common denominator of pathology in Chagas disease is the minimal rejection unit, whereby parasite-free...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02762006000500001
更新日期:2006-08-01 00:00:00
abstract::The triatomine bug Triatoma infestans probably originated in Bolivia and dispersed passively over wide areas of South America, where it is the principal vector of Trypanosoma cruzi. In the region of its probable origin this species shows colonization in two different ecotopes, so that it may be encountered in sylvatic...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762000000600025
更新日期:2000-11-01 00:00:00
abstract::A pseudogene, designated as "ps(5.8S+ITS-2)", paralogous to the 5.8S gene and internal transcribed spacer (ITS)-2 of the nuclear ribosomal DNA (rDNA), has been recently found in many triatomine species distributed throughout North America, Central America and northern South America. Among characteristics used as crite...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760140398
更新日期:2015-05-01 00:00:00
abstract::The effect of deltamethrin of the sandfly population in a focus of American Cutaneous Leishmaniasis in Viana, Espírito Santo State is described. The phlebotomine population density was determined inside and outside houses during a one year period in a treated and untreated area. The results showed a significant reduct...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761991000400004
更新日期:1991-10-01 00:00:00
abstract::In 2008, we have celebrated the centenary of the discovery of Toxoplasma gondii.Although this ubiquitous protozoan can generate devastating damage in foetuses and newborns, its treatment is the only field in which we have made little progress, despite a huge body of research, and has not yet been validated. Pregnant w...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02762009000200028
更新日期:2009-03-01 00:00:00