Comparative analysis by polymerase chain reaction amplified minicircles of kinetoplast DNA of a stable strain of Trypanosoma cruzi from São Felipe, Bahia, its clones and subclones: possibility of predominance of a principal clone in this area.

abstract：:Neisseria meningitidis retains its ability to cause endemic and hiperendemic disease in human population living in any environment, as well as localized outbreaks and massive epidemics in civilians and military personnel. In Rio de Janeiro it has been reported in the 1990s as prolonged outbreak of serogroup B and at l...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Barroso DE,Rebelo MC

更新日期：2007-09-01 00:00:00

abstract：BACKGROUND:Lutzomyia umbratilis, the vector for Leishmania guyanensis in northern South America, has been found naturally infected with L. guyanensis only in areas north of the Negro and Amazon rivers. While populations of this sand fly species are also found in areas south of these rivers, these populations have never...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Soares RP,Nogueira PM,Secundino NF,Marialva EF,Ríos-Velásquez CM,Pessoa FAC

更新日期：2018-03-01 00:00:00

abstract：:In this study, HIV-1 viral blood quantitation determined by Nucleic Acid Sequence Based Amplification (NASBA) was compared with other surrogate disease progression markers (antigen p24, CD4/CD8 cell counts and beta-2 microglobulin) in 540 patients followed up at São Paulo, SP, Brazil. HIV-1 RNA detection was statistic...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Sitnik R,Pinho JR

更新日期：1998-05-01 00:00:00

abstract：:A shift from large to small average sizes was observed in Triatoma infestans and Rhodnius domesticus between field and domestic (or laboratory) conditions of life. It was more pronounced in the female specimens, leading to a subsequent reduction of sexual size dimorphism. This feature is discussed in terms of genetic ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Dujardin J,Steinden M,Chavez T,Machane M,Schofield C

更新日期：1999-07-01 00:00:00

abstract：:The effects of radiation on the reproductive capacity and the longevity of Panstrongylus megistus were studied. An indirect correlation between longevity and radiation doses was observed. Males were more affected than females. Longevity of females submitted to 10 Gy was not different when compared to controls. Some of...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Verçosa P,Falcão PH,Furtado AF

更新日期：1993-04-01 00:00:00

abstract：:Lutzomyia georgii n. sp. and the female of L. tarapacaensis in the Series infraspinosa of the subgenus Evandromyia are described, from specimens collected in rainforest in the north of the State of Pará, Brazil. The new species was taken together with five other Evandromyia species including L. infraspinosa (sensu str...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Freitas RA,Barrett TV

更新日期：2002-03-01 00:00:00

abstract：:A 19-month mark-release-recapture study of Neotoma micropus with sequential screening for Leishmania mexicana was conducted in Bexar County, Texas, USA. The overall prevalence rate was 14.7% and the seasonal prevalence rates ranged from 3.8 to 26.7%. Nine incident cases were detected, giving an incidence rate of 15.5/...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Raymond RW,McHugh CP,Witt LR,Kerr SF

更新日期：2003-03-01 00:00:00

abstract：:A closed Lutzomyia longipalpis colony, from Ceará has been used to transmit Leishmania chagasi isolated from a fox in Pará state. The last time this colony was successfully used in similar transmission experiments was eight years (64 generations) ago indicating that this colony of Lu. longipalpis has fully maintained ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Gonçalves MD,Ryan L,Lainson R,Shaw JJ

更新日期：1985-07-01 00:00:00

abstract：:Five field populations of Musca domestica L. collected in poultry farms were bioassayed in order to detect possible resistance to the larvicide cyromazine in Brazil. The concentrations used were 0, 0.5, 0.1, 0.2, 0.4, 1, 2, 4 and 8 ppm. Three populations (Petrópolis, RJ, Montes Claros, MG and Promissão, SP) were resis...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Pinto MC,do Prado AP

更新日期：2001-07-01 00:00:00

abstract：:Toxicological and toxicogenetic effects of aqueous (tea) and hexanic fruit extract of Indigofera suffruticosa Mill. and hydroalcoholic root extract of Solanum agrarium Stendt. were evaluated in Balb C male mice intraperitoneally exposed. A hepatotoxic effect was observed just for animals treated with aqueous fruit ext...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Ribeiro LR,Bautista AR,Silva AR,Sales LA,Salvadori DM,Maia PC

更新日期：1991-01-01 00:00:00

abstract：:Molecular evidence showed 46.2% of Trypanosoma cruzi infection in Mepraia spinolai insects from North-Central Chile, which is significantly higher than previous reports of up to 26% by microscopic observation. Our results show similar infection levels among nymphal stages, ranging from 38.3 to 54.1%, indicating that y...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Botto-Mahan C,Ortiz S,Rozas M,Cattan PE,Solari A

更新日期：2005-05-01 00:00:00

abstract：:A large bibliographic survey provided data on Trypanosoma cruzi serology covering the period 1948-1984. Epidemiological-demographic methods provided an estimate of 11% for the prevalence of positive serology in Brazil, by 1984. Significant temporal trends were observed for most of the Brazilian geographical regions as...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Feitosa MF,Krieger H

更新日期：1991-04-01 00:00:00

abstract：:Mutations in the rpoB locus confer conformational changes leading to defective binding of rifampin (RIF) to rpoB and consequently resistance in Mycobacterium tuberculosis. Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) was established as a rapid screening test for the detection of mutatio...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Isfahani BN,Tavakoli A,Salehi M,Tazhibi M

更新日期：2006-09-01 00:00:00

abstract：:We analyzed prospectively 326 laboratory-confirmed, uncomplicated malarial infections (46.3% due to Plasmodium vivax, 35.3% due to P. falciparum, and 18.4% mixed-species infections) diagnosed in 162 rural Amazonians aged 5-73 years. Thirteen symptoms (fever, chills, sweating, headache, myalgia, arthralgia, abdominal p...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： da Silva-Nunes M,Ferreira MU

更新日期：2007-06-01 00:00:00

abstract：:Sand flies were collected in the central region of the state of Rondônia (W 64 degrees 30' to 63 degrees 00' and S 10 degrees 00'to 11 degrees 00') using Shannon and CDC light traps from October 1997 to August 2000. A total of 85,850 specimens representing 78 named species were captured. Of these 14 were new records f...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Gil LH,Basano SA,Souza AA,Silva MG,Barata I,Ishikawa EA,Camargo LM,Shaw JJ

更新日期：2003-09-01 00:00:00

abstract：:Nitroarylidenemalononitriles and their cyanoacetamide derivatives with remarkable anti-epimastigote properties, were synthesized attempting to obtain new 3,5-diamino-4-(5'-nitroarylidene)-4H-thiadiazine 1,1-dioxide derivatives, which in previous reports had shown anti-Trypanosoma cruzi activity. Tests to evaluate the ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Muelas-Serrano S,Le-Senne A,Fernandez-Portillo C,Nogal JJ,Ochoa C,Gomez-Barrio A

更新日期：2002-06-01 00:00:00

abstract：:Major emergency efforts are being mounted for each vector-borne disease epidemiological crisis anew, while knowledge about the biology of arthropods vectors is dwindling slowly but continuously, as is the number of field entomologists. The discrepancy between the rates of production of knowledge and its use and need f...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Casas J,Lazzari C,Insausti T,Launois P,Fouque F

更新日期：2016-11-01 00:00:00

abstract：:Two sibling species of Biomphalaria, B. tenagophila and B. occidentalis were identified using isozyme patterns obtained by horizontal gel electrophoresis. Six diagnostic enzymatic loci were identified in digestive gland homogenates. The results enable us to distinguish the species, calculate the Nei's coefficient of g...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Mascara D,Morgante JS

更新日期：1995-05-01 00:00:00

abstract：:Benznidazole (Bz) exhibits toxic side effects in animal studies and clinical use. Reductive metabolism of Bz in liver microsomes modulates the duration of its chemotherapeutic effect and its toxicity. The rate of this metabolism depends on age and is less intense in newborns and youngsters than in adults. In the prese...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Bulffer RF,Castro JA,Fanelli SL

更新日期：2011-05-01 00:00:00

abstract：:More sensitive methodologies are necessary to improve strongyloidiasis diagnosis. This study compared the sensitivities of the McMaster modified technique and polymerase chain reaction (PCR) assays, both performed in faecal samples. Lewis rats were subcutaneously infected with 4,000, 400 or 40 infective third-stage la...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Marra NM,Chiuso-Minicucci F,Machado GC,Zorzella-Pezavento SF,França TG,Ishikawa LL,Amarante AF,Sartori A,Amarante MR

更新日期：2010-02-01 00:00:00

abstract：:Chronic Chagas disease diagnosis relies on laboratory tests due to its clinical characteristics. The aim of this research was to review commercial enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) diagnostic test performance. Performance of commercial ELISA or PCR for the diagnosis of chron...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Brasil PE,Castro R,Castro Ld

更新日期：2016-01-01 00:00:00

abstract：:This paper presents the morphological, histological and ultrastructural characteristics of Myxobolus oliveirai sp. nov., a parasite of the gill filaments in Brycon hilarii from the Brazilian Pantanal. Out of 216 B. hilariispecimens examined (126 wild and 90 cultivated), 38.1% of wild specimens (n = 48) were infected. ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Milanin T,Eiras JC,Arana S,Maia AA,Alves AL,Silva MR,Carriero MM,Ceccarelli PS,Adriano EA

更新日期：2010-09-01 00:00:00

abstract：:Four diamines and three amino alcohols derived from 1-decanol, 1-dodecanol and 1,2-dodecanediol were evaluated in an in vitro assay against a mixture of trypomastigote and intracellular amastigote forms of Trypanosoma cruzi. Two of these compounds (6 and 7) showed better activity against both proliferative stages of T...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Sales Júnior PA,Rezende Júnior CO,Le Hyaric M,Almeida MV,Romanha AJ

更新日期：2014-06-01 00:00:00

abstract：:We have been interested in identifying genes that play a role in reproduction of the mosquito Aedes aegypti. Our interests are currently focused on the vitellogenin genes which in the mosquito are expressed only in the fat body in response to the insect steroid hormone, 20-hydroxyecdysone. Four of the five vitellogeni...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Hamblin MT,Marx JL,Wolfner MF,Hagedorn HH

更新日期：1987-01-01 00:00:00

abstract：BACKGROUND:Chagas disease is a public health problem caused by infection with the protozoan Trypanosoma cruzi. There is currently no effective therapy for Chagas disease. Although there is some evidence for the beneficial effect of bone marrow-derived cells in chagasic disease, the mechanisms underlying their effects i...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Irion CI,Paredes BD,Brasil GV,Cunha STD,Paula LF,Carvalho AR,Carvalho ACC,Carvalho AB,Goldenberg RCDS

更新日期：2017-08-01 00:00:00

abstract：:Protection against Fasciola hepatica in goats immunized with a synthetic recombinant antigen from Schistosoma mansoni fatty acid-binding protein 14 (rSm14) was investigated by assessing worm burdens, serum levels of hepatic enzymes, faecal egg count and hepatic damage, which was evaluated using gross and microscopic m...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Mendes RE,Zafra R,Pérez-Ecija RA,Buffoni L,Martínez-Moreno A,Tendler M,Pérez J

更新日期：2010-08-01 00:00:00

abstract：:This study's objective was to search for Cryptosporidium sp. in diarrheic feces from children aged zero to 12 years and cared for at medical units within Universidade Federal de Uberlândia or at a private practice in Uberlândia, State of Minas Gerais, Brazil, from September 1992 to August 1993. Three fecal samples pre...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Gennari-Cardoso ML,Costa-Cruz JM,de Castro E,Lima LM,Prudente DV

更新日期：1996-09-01 00:00:00

abstract：:There is a paucity of studies on the cell biology of Sporothrix luriei, the less common of the pathogenic Sporothrix species worldwide. The production of DHN-melanin, eumelanin, and pyomelanin were evaluated on the mycelial and yeast forms of the S. luriei ATCC 18616 strain. The mycelial form of this species produced ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Cruz ILR,Figueiredo-Carvalho MHG,Zancopé-Oliveira RM,Almeida-Paes R

更新日期：2018-01-01 00:00:00

abstract：:The determination of amino acid changes in the envelope protein by direct sequencing of either genomic RNA or PCR-amplified cDNA fragments provides useful informations for assessing the genetic variability and the geographic distribution of the actually most widespread dengue-2 serotype. The possible link of variation...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Deubel V

更新日期：1992-01-01 00:00:00

abstract：:Chagas cardiomyopathy is the most frequent and most severe manifestation of chronic Chagas disease, and is one of the leading causes of morbidity and death in Latin America. Although the pathogenesis of Chagas cardiomyopathy is incompletely understood, it may involve several mechanisms, including parasite-dependent my...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Rassi A Jr,Marin JA Neto,Rassi A

更新日期：2017-03-01 00:00:00