Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells.

Abstract:

:In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role.

journal_name

Mem Inst Oswaldo Cruz

authors

Rocha FF,Araujo RN,Silva LM,Gontijo NF,Pereira MH

doi

10.1590/s0074-02762010000200004

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

132-6

issue

2

eissn

0074-0276

issn

1678-8060

pii

S0074-02762010000200004

journal_volume

105

pub_type

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