Analysis of E2 gene integrity in HPV16 and HPV58 viruses isolated from women with cervical pathology.

Abstract:

:Integration of human papillomavirus (HPV) DNA into human cells accompanied by the disruption of the viral genome has been described as a prerequisite for cancer development. This study aimed to investigate E2 gene integrity of HPV16 and HPV58 viruses isolated from infected women with cervical lesions. Forty-two HPV16- and 31 HPV58-positive samples were analysed. E2 integrity was assumed when all fragments covering the E2 gene were amplified with specific polymerase chain reaction primers. Overall, in 59% of the samples, at least one fragment was not amplified in HPV16- (57%) and HPV58-positive samples (61%). Samples from high-grade squamous intraepithelial lesions had the highest frequency of E2 gene disruptions (73%), followed by samples from low-grade squamous intraepithelial lesions (63%) and, finally, samples from invasive cervical cancer (35%). Association between the integrity status of the E2 gene, and lesion grade was assessed by the chi-squared test applied to the combined set of viruses (p = 0.6555) or to populations of the same virus type (HPV58, p = 0.3101; HPV16, p = 0.3024). In conclusion, in this study, no association was found between the presence of E2 gene disruptions and the grade of cervical lesions caused by HPV16 and HPV58.

journal_name

Mem Inst Oswaldo Cruz

authors

González-Losa MD,Puerto-Solis M,Tenorio Ruiz J,Rosado-López AI,Hau-Aviles O,Ayora-Talavera G,Cisneros-Cutz I,Conde-Ferráez L

doi

10.1590/0074-02760160269

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

770-773

issue

12

eissn

0074-0276

issn

1678-8060

pii

S0074-02762016005022103

journal_volume

111

pub_type

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