Anesthesia of Biomphalaria spp. (Mollusca, Gastropoda): sodium pentobarbital is the drug of choice.

Abstract:

:The anesthetic effect of some water-soluble anesthesic or narcotic drugs currently used in mice was tested in molluscs of the Biomphalaria genus. Sodium thiopental was very toxic to the snails resulting in high rates of mortality in all the treatment schedules tested. Cetamine base, at concentration of 0.25 mg/ml of water, resulted in partial snail anesthesia (40% of snails were anesthetized) only after 20 h of exposition. The association of Cetamine base with Tiazine chloridrate did not improve the anesthesic effect, and higher concentrations of these drugs were toxic to the snails. Sodium pentobarbital at 0.4 mg/ml in water for 8 h was the best treatment schedule to anesthetize Biomphalaria snails. In this schedule, the snails were anesthetized without any toxic effect. The procedure provides a powerful tool for in vivo studies that demande a complete state of snail anesthesia.

journal_name

Mem Inst Oswaldo Cruz

authors

Martins-Sousa R,Negrão-Corrêa D,Bezerra F,Coelho P

doi

10.1590/s0074-02762001000300019

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

391-2

issue

3

eissn

0074-0276

issn

1678-8060

pii

S0074-02762001000300019

journal_volume

96

pub_type

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