Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections.

Abstract:

:An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.

journal_name

Mem Inst Oswaldo Cruz

authors

Bentes GA,Lanzarini NM,Lima LR,Manso PP,da Silva Ados S,Mouta Junior Sda S,Guimarães JR,de Moraes MT,Pelajo-Machado M,Pinto MA

doi

10.1590/0074-02760140457

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

577-9

issue

4

eissn

0074-0276

issn

1678-8060

pii

S0074-02762015005040457

journal_volume

110

pub_type

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