The Pf332 gene codes for a megadalton protein of Plasmodium falciparum asexual blood stages.

Abstract:

:We characterized the Plasmodium falciparum antigen 332 (Ag332) which is specifically expressed during the asexual intraerythrocytic cycle of the parasite. The corresponding Pf332 gene has been located in the subtelomeric region of chromosome 11. Furthermore, it is present in all strains so far analyzed and shows marked restriction length fragment polymorphism. Partial sequence and restriction endonuclease digestion of cloned fragments revealed that the Pf332 gene is composed of highly degenerated repeats rich in glutamic acid. Mung been nuclease digestion and Northern blot analysis suggested that the Pf332 gene codes for a protein of about 700 kDa. These data were further confirmed by Western blot and immunoprecipitation of parasites extracts with an antiserum raised against a recombinant clone expressing part of the Ag332. Confocal immunofluorescence showed that Ag332 is translocated from the parasite to the surface of infected red blood cells within vesicle-like structures. In addition, Ag332 was detected on the surface of monkey erythrocytes infected with Plasmodium falciparum.

journal_name

Mem Inst Oswaldo Cruz

authors

Mattei D,Scherf A

doi

10.1590/s0074-02761992000700026

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

163-8

eissn

0074-0276

issn

1678-8060

pii

S0074-02761992000700026

journal_volume

87 Suppl 3

pub_type

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