Abstract：
:The high level of protection elicited in rodents and primates by the radiation-attenuated schistosome vaccine gives hope that a human vaccine relying on equivalent mechanisms is feasible. In humans, a vaccine would be undoubtedly administered to previously or currently infected individuals. We have therefore used the olive baboon to investigate whether vaccine-induced immunity is compromised by a schistosome infection. We showed that neither a preceding infection, terminated by chemotherapy, nor an ongoing chronic infection affected the level of protection. Whilst IgM responses to vaccination or infection were short-lived, IgG responses rose with each successive exposure to the vaccine. Such a rise was obscured by responses to egg deposition in already-infected animals. In human trials it would be necessary to use indirect estimates of infection intensity to determine vaccine efficacy. Using worm burden as the definitive criterion, we demonstrated that the surrogate measures, fecal eggs, and circulating antigens, consistently overestimated protection. Regression analysis of the surrogate parameters on worm burden revealed that the principal reason for overestimation was the threshold sensitivity of the assays. If we extrapolate our findings to human schistosomiasis mansoni, it is clear that more sensitive indirect measures of infection intensity are required for future vaccine trials.
journal_name
Mem Inst Oswaldo Cruzjournal_title
Memorias do Instituto Oswaldo Cruzauthors
Coulson PS,Kariuki TMdoi
10.1590/s0074-02762006000900061subject
Has Abstractpub_date
2006-09-01 00:00:00pages
369-72eissn
0074-0276issn
1678-8060pii
S0074-02762006000900061journal_volume
101 Suppl 1pub_type
杂志文章abstract：:We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequences. ESTs can be used to identify genes on the basis of their homology with sequences from other species deposit...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02761995000200015
更新日期：1995-03-01 00:00:00
abstract：:Helmintex is a sensitive method used for detecting Schistosoma mansoni eggs. Here, we describe the observed frequency of six proposed criteria associated with the identification of S. mansoni eggs prepared with the Helmintex method and stained with ninhydrin. The efficacy of these criteria in classifying S. mansoni eg...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/0074-02760180529
更新日期：2019-01-01 00:00:00
abstract：:Triatoma nitida was found in 14 (0.4%) out of 3,726 houses located in six departments across Guatemala, which were surveyed from 1994 to 1998 by the man-hour collection method. Compared to previous information, the distribution of T. nitida in Guatemala has increased from five to nine departments; the species is prese...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762003000100006
更新日期：2003-01-01 00:00:00
abstract：:The objective of this study was to evaluate the feeding behavior of Triatoma vitticeps through the identification of its food sources and the characterization of the blood ingestion process. In addition, we aimed to verify if the saliva of this vector interferes with the perception of the host during the feedings by c...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762011000100003
更新日期：2011-02-01 00:00:00
abstract：:The author studied by the complement fixation test the influenza virus strains isolated in Rio de Janeiro during the 1973 epidemic. He prepared immunesera in hamsters by the inoculation of the allantoic fluid from infected chick embryos with each of the 7 isolated strains and the standard strains. The soluble antigens...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02761976000200004
更新日期：1976-01-01 00:00:00
abstract：:In this study we investigated the larval dispersal associated with larval predation in experimental populations of Chrysomya albiceps and Cochliomyia macellaria. Frequency distribution of sampling units (G test) in the substrate was used to evaluate variation in larval dispersal. An experimental acrylic channel (1 x 0...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762002000800013
更新日期：2002-12-01 00:00:00
abstract：:The ultrastructure of endogenous stages of Eimeria ninakohlyakimovae was observed in epithelial cells of cecum and colon crypts from a goat experimentally infected with 2.0 x 10(5) oocysts/kg. The secondary meronts developed above the nucleus of the host cell. The nucleus first divides and merozoites then form on the ...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02761997000400017
更新日期：1997-07-01 00:00:00
abstract：:The restriction fragment length polymorphism of the 195 bp repeated DNA sequence of Trypanosoma cruzi was analyzed among 23 T. cruzi stocks giving a reliable picture of the whole phylogenetic variability of the species. The profiles observed with the enzymes Hinf I and Hae III were linked together and supported the ex...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02761999000300008
更新日期：1999-05-01 00:00:00
abstract：:Chagas cardiomyopathy is the most frequent and most severe manifestation of chronic Chagas disease, and is one of the leading causes of morbidity and death in Latin America. Although the pathogenesis of Chagas cardiomyopathy is incompletely understood, it may involve several mechanisms, including parasite-dependent my...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/0074-02760160334
更新日期：2017-03-01 00:00:00
abstract：:In this study, IgA1 levels in the milk and serum of puerperae were compared and a correlation was established between the levels of this immunoglobulin and the occurrence of parasitism. Eighty-three paired milk and serum samples were obtained from puerperal and IgA1 levels were analyzed. In addition, the presence of i...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762008000500020
更新日期：2008-08-01 00:00:00
abstract：:Thirteen species of helminth parasites were recovered from six species of charadriid shorebirds (Aves: Charadriiformes) from Belize: the ruddy turnstone, Arenaria interpres, the snowy plover, Charadrius alexandrinus, the semipalmated plover, C. semipalmatus, the killdeer, C. vociferus, the white-rumped sandpiper, Cali...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762001000600015
更新日期：2001-08-01 00:00:00
abstract：:Living in close association with a vertebrate host and feeding on its blood requires different types of adaptations, including behavioural adjustments. Triatomines exhibit particular traits associated with the exploitation of their habitat and food sources and these traits have been the subject of intense analysis. Ma...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762009000900011
更新日期：2009-07-01 00:00:00
abstract：:We describe a technique to separate male and female pupae of sand flies. This has reduced the labour to separate flies after emergence and also allows the isolation of unmated adults for behavioural and physiological studies. ...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762000000400004
更新日期：2000-07-01 00:00:00
abstract：:As pyrethroids are presently the favored group of insecticides to control triatomines, we performed a series of bioassays to determine the intrinsic activity of some of the main compounds used in the control campaigns, against five of the main species of triatomines to be controlled. Comparing the insecticides it can ...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/S0074-02761999000700084
更新日期：1999-01-01 00:00:00
abstract：:BACKGROUND Fluctuations in climate have been associated with variations in mosquito abundance. OBJECTIVES To analyse the influence of precipitation, temperature, solar radiation, wind speed and humidity on the oviposition dynamics of Aedes aegypti in three distinct environmental areas (Brasília Teimosa, Morro da Conce...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/0074-02760200046
更新日期：2020-01-01 00:00:00
abstract：:The authors describe and compare the morphological and ultrastructural characteristics of eggs from the three most recent described species of the genus Rhodnius Stål, 1859, which have not previously been studied. These species are Rhodnius colombiensis (Mejia, Galvão & Jurberg 1999), Rhodnius milesi (Carcavallo, Roch...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762009000700013
更新日期：2009-11-01 00:00:00
abstract：:The cellular nature of the infiltrate in cutaneous lesion of rhesus monkeys experimentally infected with Leishmania (L.) amazonensis was characterized by immunohistochemistry. Skin biopsies from infected animals with active or healing lesions were compared to non-infected controls (three of each type) to quantitate in...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762000000200012
更新日期：2000-03-01 00:00:00
abstract：:The aim of the present study was to identify specific markers that mirror liver fibrosis progression as an alternative to biopsy when biopsy is contraindicated, especially in children. After liver biopsies were performed, serum samples from 30 hepatitis C virus (HCV) paediatric patients (8-14 years) were analysed and ...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/0074-0276130139
更新日期：2013-11-01 00:00:00
abstract：:Osteoarthritis (OA) is a common painful inflammatory condition occurring mainly in the later half of life. Hip and knee are the joints mostly affected. Petiveria alliacea (tipi) popularly known as an anti-rheumatic medicine, has been used by OA patients to relief pain. This one-week cross-over double-blind trial has p...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1590/s0074-02761991000600054
更新日期：1991-01-01 00:00:00
abstract：:The extent of genomic variability of clones of Schistosoma mansoni obtained from field isolates was compared with that of strains that have been laboratory maintained. Analysis was undertaken using randomly amplified polymorphic DNAs (RAPDs) generated with three primers. Phenograms showing the similarity among the clo...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02761997000300019
更新日期：1997-05-01 00:00:00
abstract：:To assess differentiation and relationships between Anopheles lesteri and Anopheles paraliae we established three and five iso-female lines of An. lesteri from Korea and An. paraliae from Thailand, respectively. These isolines were used to investigate the genetic relationships between the two taxa by crossing experime...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/S0074-02762013000300009
更新日期：2013-05-01 00:00:00
abstract：:Neisseria meningitidis retains its ability to cause endemic and hiperendemic disease in human population living in any environment, as well as localized outbreaks and massive epidemics in civilians and military personnel. In Rio de Janeiro it has been reported in the 1990s as prolonged outbreak of serogroup B and at l...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762007005000104
更新日期：2007-09-01 00:00:00
abstract：:Sixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochís vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02761999000400014
更新日期：1999-07-01 00:00:00
abstract：:Between 1985 and 2000, epidemiological surveys of the American tegumentary leishmaniasis (ATL) were carried out in several rural and urban communities in Espírito Santo, Brazil. A total of 100 stocks of Leishmania (comprising isolates from both human and canine hosts with ATL) were identified by two methods of molecul...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762003000800004
更新日期：2003-12-01 00:00:00
abstract：:This study updates the geographic distributions of phlebotomine species in Central-West Brazil and analyses the climatic factors associated with their occurrence. The data were obtained from the entomology services of the state departments of health in Central-West Brazil, scientific collections and a literature revie...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/0074-02760140462
更新日期：2015-06-01 00:00:00
abstract：:Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral pathogens from artifici...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762012000100002
更新日期：2012-02-01 00:00:00
abstract：:Several parasitological studies carried out in El Salvador between 2000-2012 showed a higher frequency of acute cases of Chagas disease than that in other Central American countries. There is an urgent need for improved Chagas disease surveillance and vector control programs in the provinces where acute Chagas disease...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/0074-0276140416
更新日期：2014-04-01 00:00:00
abstract：:Integration of human papillomavirus (HPV) DNA into human cells accompanied by the disruption of the viral genome has been described as a prerequisite for cancer development. This study aimed to investigate E2 gene integrity of HPV16 and HPV58 viruses isolated from infected women with cervical lesions. Forty-two HPV16-...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/0074-02760160269
更新日期：2016-12-01 00:00:00
abstract：:Sixteen species of ectoparasites were collected from 50 wild rodents, from August 1990 to August 1991, in an area of Araucaria augustifolia forest, in the municipality of Tijucas do Sul, State of Paraná, Brazil. Ectoparasites infested 98% of the rodents, with the highest indices of infestation found in the drycool sea...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02761998000600003
更新日期：1998-11-01 00:00:00
abstract：:The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients e...
journal_title：Memorias do Instituto Oswaldo Cruz
pub_type： 杂志文章
doi：10.1590/s0074-02762010000100018
更新日期：2010-02-01 00:00:00
