Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin.

Abstract:

:Helmintex is a sensitive method used for detecting Schistosoma mansoni eggs. Here, we describe the observed frequency of six proposed criteria associated with the identification of S. mansoni eggs prepared with the Helmintex method and stained with ninhydrin. The efficacy of these criteria in classifying S. mansoni eggs when applied in various combinations was also examined. Nine observers registered the presence or absence of 6 different criteria in 100 eggs using a microscope at 100x magnification. Ninhydrin purple, which was frequently observed, was the criterion associated with the lowest inter-observer variability. At least three criteria were associated with a significantly better performance in egg identification. In conclusion, ninhydrin staining and a combination of criteria are recommended for microscope examination of faecal sediments.

journal_name

Mem Inst Oswaldo Cruz

authors

de Souza RP,Favero V,Pascoal VF,Lindholz C,Bittencourt HR,Graeff-Teixeira C

doi

10.1590/0074-02760180529

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

e180529

eissn

0074-0276

issn

1678-8060

pii

S0074-02762019000100409

journal_volume

114

pub_type

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