Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library.

Abstract:

:We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequences. ESTs can be used to identify genes on the basis of their homology with sequences from other species deposited in DNA or protein databases. Transcripts with sequences without matches in the databases may represent novel parasite-specific genes. This approach has shown to be very efficient and in less than two years a broad range of novel genes has already been ascertained, more than doubling the number of known S. mansoni genes.

journal_name

Mem Inst Oswaldo Cruz

authors

Franco GR,Simpson AJ,Pena SD

doi

10.1590/s0074-02761995000200015

subject

Has Abstract

pub_date

1995-03-01 00:00:00

pages

215-6

issue

2

eissn

0074-0276

issn

1678-8060

journal_volume

90

pub_type

杂志文章
  • [Angiostrongylus vasorum (Baillet, 1866) Nematoda: Protostrongylidae, in dogs of Minas Gerais, Brazil].

    abstract::For the first time Angiostrongylus vasorum in Canis familiaris in Minas Gerais State, Brazil, is described. The description and measurements of three males and ten females are presented together with a diagram of the parasite. ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761985000200015

    authors: dos Santos Lima W,de Araujo Costa HM,Guimarães MP,Leite AC

    更新日期:1985-04-01 00:00:00

  • Therapeutic switching: from antidermatophytic essential oils to new leishmanicidal products.

    abstract::This study examined whether the antidermatophytic activity of essential oils (EOs) can be used as an indicator for the discovery of active natural products against Leishmania amazonensis. The aerial parts of seven plants were hydrodistilled. Using broth microdilution techniques, the obtained EOs were tested against th...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760140332

    authors: Houël E,Gonzalez G,Bessière JM,Odonne G,Eparvier V,Deharo E,Stien D

    更新日期:2015-02-01 00:00:00

  • Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations.

    abstract::The aim of this study was to determine the presence of Helicobacter pylori cytotoxin-associated gene (cagA)/vacuolating cytotoxin gene (vacA) among patients with chronic gastritis in Cuba and Venezuela. Gastric antrum biopsies were taken for culture, DNA extraction and PCR analysis. Amplification of vacA and cagA segm...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000300016

    authors: Ortiz-Princz D,Guariglia-Oropeza V,Avila M,Correnti M,Perrone M,Gutierrez B,Torres J,Megraud F,Cavazza ME

    更新日期:2010-05-01 00:00:00

  • Survival of tubercle bacilli in heat-fixed and stained sputum smears.

    abstract::We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputu...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000200024

    authors: Cardoso CL,Giacomelli LR,Helbel C,Sant'Ana JJ,Martins FM,Barreto AM

    更新日期:2001-02-01 00:00:00

  • EVI antibodies in patients with Chagas' disease: relationship with anti-Trypanosoma cruzi immunoglobulins and effects of specific treatment.

    abstract::Antibodies against heart vascular structures and striated muscle cells interstitium (EVI antibodies) persist in Chagas' disease patients who had been cured by specific treatment as demonstrated by negative xenodiagnosis, conventional serology (CS) and complement mediated lysis (CoML). On the other hand, EVI antibodies...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761983000400007

    authors: Brener Z,Ramirez LE,Krettli AU,Cançado JR

    更新日期:1983-10-01 00:00:00

  • Mechanisms of cell accumulation induced by Mycobacterium bovis BCG.

    abstract::Mycobacteria, specially Mycobacterium tuberculosis are among the micro-organisms that are increasing dramatically the number of infections with death, all over the world. A great number of animal experimental models have been proposed to investigate the mechanisms involved in the host response against these intracellu...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761997000800033

    authors: Menezes-de-Lima-Júnior O,Henriques M das G

    更新日期:1997-01-01 00:00:00

  • Recent advances and prospective researches on molecular epidemiology of dengue viruses.

    abstract::The determination of amino acid changes in the envelope protein by direct sequencing of either genomic RNA or PCR-amplified cDNA fragments provides useful informations for assessing the genetic variability and the geographic distribution of the actually most widespread dengue-2 serotype. The possible link of variation...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761992000900021

    authors: Deubel V

    更新日期:1992-01-01 00:00:00

  • Intracellular polyamine pools, oligopeptide-binding protein A expression, and resistance to aminoglycosides in Escherichia coli.

    abstract::The role of intracellular free polyamine (putrescine and spermidine) pools in multiple resistance to aminoglycoside antibiotics was investigated among in vitro selected kanamycin-resistant Escherichia coli J53 mutants expressing diminished oligopeptide-binding protein (OppA) levels and/or defective ornithine decarboxy...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762005000700020

    authors: Acosta MB,Ferreira RC,Ferreira LC,Costa SO

    更新日期:2005-11-01 00:00:00

  • In vitro effects of citral on trypanosoma cruzi metacyclogenesis.

    abstract::Citral, the main constituent of lemongrass (Cymbopogon citratus) essential oil, was added to Trypanosoma cruzi cultures grown in TAU3AAG medium to observe the effect on the epimastigote-to-trypomastigote differentiation process (metacyclogenesis). Our results showed that citral (20 μg/mL) did not affect epimastigote v...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000800012

    authors: Cardoso J,Soares MJ

    更新日期:2010-12-01 00:00:00

  • Temporal abundance of Aedes aegypti in Manaus, Brazil, measured by two trap types for adult mosquitoes.

    abstract::A longitudinal study was conducted in Manaus, Brazil, to monitor changes of adult Aedes aegypti (L.) abundance. The objectives were to compare mosquito collections of two trap types, to characterise temporal changes of the mosquito population, to investigate the influence of meteorological variables on mosquito collec...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276140234

    authors: Degener CM,Ázara TM,Roque RA,Codeço CT,Nobre AA,Ohly JJ,Geier M,Eiras ÁE

    更新日期:2014-12-01 00:00:00

  • Taenia solium metacestode immunodominant peptides recognized by IgG antibodies in cerebrospinal fluid and serum paired samples from patients with active and inactive neurocysticercosis.

    abstract::The aim of this study was to test if serological distinction between patients with active and inactive neurocysticercosis (NCC), could be accomplished by the recognition of immunodominant peptides in total saline antigenic extract of Taenia solium metacestodes by IgG antibody in cerebrospinal fluid (CSF) and serum pai...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000084

    authors: Barcelos IS,Moura LP,Costa VP,Ferreira MS,Costa-Cruz JM

    更新日期:2007-09-01 00:00:00

  • Commercial enzyme-linked immunosorbent assay versuspolymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis.

    abstract::Chronic Chagas disease diagnosis relies on laboratory tests due to its clinical characteristics. The aim of this research was to review commercial enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) diagnostic test performance. Performance of commercial ELISA or PCR for the diagnosis of chron...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,meta分析,评审

    doi:10.1590/0074-02760150296

    authors: Brasil PE,Castro R,Castro Ld

    更新日期:2016-01-01 00:00:00

  • Comparison of two PCR methods for detection of Leptospira interrogans in formalin-fixed and paraffin-embedded tissues.

    abstract::In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with sever...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000100012

    authors: D'Andrea A,Martinez YZ,Alduina R,Monteverde V,Molina CF,Vitale M

    更新日期:2012-02-01 00:00:00

  • Similarity between the association factor of ribosomal subunits and the protein Stm1p from Saccharomyces cerevisiae.

    abstract::A ribosome association factor (AF) was isolated from the yeast Sacchharomyces cerevisiae. Partial amino acid sequence of AF was determined from its fragment of 25 kDa isolated by treating AF with 2-(2-nitrophenylsulfenyl)-3-methyl-3'-Bromoindolenine (BNPS-skatole). This sequence has a 86% identity to the product of th...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762004000700012

    authors: Correia H,Medina R,Hernández A,Bustamante E,Chakraburtty K,Herrera F

    更新日期:2004-11-01 00:00:00

  • Susceptibility of Phytomonas serpens to calpain inhibitors in vitro: interference on the proliferation, ultrastructure, cysteine peptidase expression and interaction with the invertebrate host.

    abstract::A pleiotropic response to the calpain inhibitor MDL28170 was detected in the tomato parasite Phytomonas serpens. Ultrastructural studies revealed that MDL28170 caused mitochondrial swelling, shortening of flagellum and disruption of trans Golgi network. This effect was correlated to the inhibition in processing of cru...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160270

    authors: Oliveira SS,Gonçalves DS,Garcia-Gomes AD,Gonçalves IC,Seabra SH,Menna-Barreto RF,Lopes AH,D'Avila-Levy CM,Santos AL,Branquinha MH

    更新日期:2017-01-01 00:00:00

  • [Microbial resistance of Salmonella agona from various regions of Brazil].

    abstract::The object of the investigation was the evaluation of the susceptibility to antibiotic and chemotherapeutic agents of 240 strains of Salmonella agona isolated from different sources (human, food and environment) obtained from five Brazilian states (Minas Gerais, São Paulo, Rio de Janeiro, Pernambuco and Rio Grande do ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761986000100002

    authors: Solari CA,dos Reis EM,Dias JC,Hofer E

    更新日期:1986-01-01 00:00:00

  • Veterinary vaccines against Toxoplasma gondii.

    abstract::Toxoplasma gondii has a very wide intermediate host range and is thought to be able to infect all warm blooded animals. The parasite causes a spectrum of different diseases and clinical symptoms within the intermediate hosts and following infection most animals develop adaptive humoral and cell-mediated immune respons...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02762009000200018

    authors: Innes EA,Bartley PM,Maley S,Katzer F,Buxton D

    更新日期:2009-03-01 00:00:00

  • Prevalence and genotypes of GB virus C/hepatitis G virus among blood donors in Central Brazil.

    abstract::A survey was conducted in a blood donor population of Central Brazil aiming to investigate the prevalence of GB virus C (GBV-C)/hepatitis G virus (HGV) infection and also to analyze the virus genotypes distribution. A total of 241 voluntary blood donors were interviewed at the State Blood Bank in Goiânia, State of Goi...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000700005

    authors: Oliveira LA,Martins RM,Carneiro MA,Teles SA,Silva SA,Cardoso DD,Lampe E,Yoshida CF

    更新日期:2002-10-01 00:00:00

  • The influence of meteorological variables on the oviposition dynamics of Aedes aegypti (Diptera: Culicidae) in four environmentally distinct areas in northeast Brazil.

    abstract::BACKGROUND Fluctuations in climate have been associated with variations in mosquito abundance. OBJECTIVES To analyse the influence of precipitation, temperature, solar radiation, wind speed and humidity on the oviposition dynamics of Aedes aegypti in three distinct environmental areas (Brasília Teimosa, Morro da Conce...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760200046

    authors: Santos ICDS,Braga C,de Souza WV,de Oliveira ALS,Regis LN

    更新日期:2020-01-01 00:00:00

  • On two nematodes from Brazilian birds and description of a new species (Acuarioidea, Schistorophinae) parasitizing Laterallus viridis (Müller, 1776) (Gruiformes, Rallidae).

    abstract::The present paper reports acuarioid nematodes recovered from avian hosts. A new species of the genus Schistorophus Railliet, 1916 is proposed based mainly on findings referring to ptilina, spicules and vagina. Ancyracanthopsis coronata (Molin, 1860) Chabaud & Petter, 1959 is referred again in Brazil since its proposit...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761999000600008

    authors: Pinto RM,Vicente JJ,Muniz-Pereira LC,Noronha D

    更新日期:1999-11-01 00:00:00

  • Comparison of some molecular-genetic techniques for identification of Leishmania circulating in natural foci of zoonotic cutaneous leishmaniasis in the central Asia region.

    abstract::Different molecular-genetic methods were used to identify a cohort of Leishmania strains from natural foci of zoonotic cutaneous leishmaniasis located in Central Asia, on the former USSR territory. The results obtained using isoenzymes, PCR, restriction fragment length polymorphisms of kDNA and molecular hybridization...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761997000100023

    authors: Strelkova MV,Pacheco RS,Bulat SA,Rakitskaya TA

    更新日期:1997-01-01 00:00:00

  • Experimental infection of Calomys callosus (Rodentia, Cricetidae) by Toxoplasma gondii.

    abstract::Calomys callosus, Rengger 1830 (Rodentia, Cricetidae), a wild rodent found in Central Brazil, was studied to investigate its susceptibility to Toxoplasma gondii experimental infection and its humoral immune response against this protozoa. The electrophoretic profile of the serum proteins of C. callosus showed that IgG...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000100018

    authors: Favoreto-Júnior S,Ferro EA,Clemente D,Silva DA,Mineo JR

    更新日期:1998-01-01 00:00:00

  • A new approach for potential drug target discovery through in silico metabolic pathway analysis using Trypanosoma cruzi genome information.

    abstract::The current drug options for the treatment of chronic Chagas disease have not been sufficient and high hopes have been placed on the use of genomic data from the human parasite Trypanosoma cruzi to identify new drug targets and develop appropriate treatments for both acute and chronic Chagas disease. However, the lack...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000800006

    authors: Alves-Ferreira M,Guimarães AC,Capriles PV,Dardenne LE,Degrave WM

    更新日期:2009-12-01 00:00:00

  • The effectiveness of tipi in the treatment of hip and knee osteoarthritis--a preliminary report.

    abstract::Osteoarthritis (OA) is a common painful inflammatory condition occurring mainly in the later half of life. Hip and knee are the joints mostly affected. Petiveria alliacea (tipi) popularly known as an anti-rheumatic medicine, has been used by OA patients to relief pain. This one-week cross-over double-blind trial has p...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1590/s0074-02761991000600054

    authors: Ferraz MB,Pereira RB,Coelho Andrade LE,Atra E

    更新日期:1991-01-01 00:00:00

  • alpha-glycerophosphate dehydrogenase activity in flight muscles of triatomine bugs Panstrongylus megistus and Triatoma sordida.

    abstract::The alpha-glycerophosphate dehydrogenase (alpha-GPDH) activity in flight muscles of Panstrongylus megistus and Triatoma sordida, vectors of Chagas disease in Brazil, was studied. Both species showed higher enzymatic activities in fliers than in non-fliers insects. T. sordida exhibited a higher proportion of flier inse...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000500016

    authors: Soares RP,Santoro MM

    更新日期:2000-09-01 00:00:00

  • Mansonella ozzardi in Brazil: prevalence of infection in riverine communities in the Purus region, in the state of Amazonas.

    abstract::This study was undertaken to investigate the prevalence of Mansonella ozzardi infection and to estimate the parasitic infection rate (PIR) in simuliid black flies in the municipality of Pauini, Amazonas, Brazil. We used thick blood films to examine 921 individuals in 35 riverine communities along the Pauini and Purus ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000100012

    authors: Medeiros JF,Py-Daniel V,Barbosa UC,Izzo TJ

    更新日期:2009-02-01 00:00:00

  • Toxicological and toxicogenetic effects of plants used in popular medicine and in cattle food.

    abstract::Toxicological and toxicogenetic effects of aqueous (tea) and hexanic fruit extract of Indigofera suffruticosa Mill. and hydroalcoholic root extract of Solanum agrarium Stendt. were evaluated in Balb C male mice intraperitoneally exposed. A hepatotoxic effect was observed just for animals treated with aqueous fruit ext...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761991000600022

    authors: Ribeiro LR,Bautista AR,Silva AR,Sales LA,Salvadori DM,Maia PC

    更新日期:1991-01-01 00:00:00

  • Ultrasound monitoring of structural urinary tract disease in Schistosoma haematobium infection.

    abstract::A major advance in our understanding of the natural history of Schistosoma haematobium-related morbidity has come through the introduction of the portable ultrasound machines for non-invasive examination of the kidneys and bladder. With the use of generators or battery packs to supply power in non-clinical field setti...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000900028

    authors: King CH

    更新日期:2002-01-01 00:00:00

  • Hepatitis C virus quantification in serum and saliva of HCV-infected patients.

    abstract::The hepatitis C virus (HCV) can be detected in blood and other bodily fluids, such as saliva, semen and gastric juices. The aim of this study was to compare the HCV viral loads in the serum and saliva of infected patients. Twenty-nine patients with detectable HCV RNA in their serum and saliva were included in this stu...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000500016

    authors: Menezes GB,Pereira FA,Duarte CA,Carmo TM,Silva Filho HP,Zarife MA,Krieger MA,Reis EA,Reis MG

    更新日期:2012-08-01 00:00:00

  • Clinical-epidemiologic profile of the schistosomal myeloradiculopathy in Pernambuco, Brazil.

    abstract::This was a retrospective descriptive study on a series of cases of schistosomal myeloradiculopathy (SMR) and the aim was to investigate the incidence of this disease and its clinical and epidemiological characteristics in cases diagnosed at three healthcare units in Pernambuco, Brazil between 1994-2006. The data were ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000400017

    authors: Araújo KC,Silva Cda R,Santos AG,Barbosa CS,Ferrari TC

    更新日期:2010-07-01 00:00:00