Comparison of two PCR methods for detection of Leptospira interrogans in formalin-fixed and paraffin-embedded tissues.

Abstract:

:In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with several bacterial suspensions. PCR was performed on the same tissues before and after the formalin-fixed, paraffin-embedding procedure (FFPE tissues). The 23S rDNA PCR detected all fresh and FFPE positive tissues while the 16S rDNA-based protocol detected primarily the positive fresh tissues. Both methods are specific for pathogenic L. interrogans. The 23S-based PCR method successfully detected Leptospira in four dubious cases of human leptospirosis from archival tissue specimens and one leptospirosis-positive canine specimen. A sensitive method for leptospirosis identification in FFPE tissues would be a useful tool to screen histological specimen archives and gain a better assessment of human leptospirosis prevalence, especially in tropical countries, where large outbreaks can occur following the rainy season.

journal_name

Mem Inst Oswaldo Cruz

authors

D'Andrea A,Martinez YZ,Alduina R,Monteverde V,Molina CF,Vitale M

doi

10.1590/s0074-02762012000100012

subject

Has Abstract

pub_date

2012-02-01 00:00:00

pages

85-8

issue

1

eissn

0074-0276

issn

1678-8060

pii

S0074-02762012000100012

journal_volume

107

pub_type

杂志文章
  • Access to diagnosis and treatment of Chagas disease/infection in endemic and non-endemic countries in the XXI century.

    abstract::In this article, Médicos Sin Fronteras (MSF) Spain faces the challenge of selecting, piecing together, and conveying in the clearest possible way, the main lessons learnt over the course of the last seven years in the world of medical care for Chagas disease. More than two thousand children under the age of 14 have be...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000081

    authors: Villa L,Morote S,Bernal O,Bulla D,Albajar-Vinas P

    更新日期:2007-10-30 00:00:00

  • First detection of dengue virus in the saliva of immunocompetent murine model.

    abstract::The lack of an experimental animal model for the study of dengue pathogenesis is a limiting factor for the development of vaccines and drugs. In previous studies, our group demonstrated the susceptibility of BALB/c mice to infection by dengue virus (DENV) 1 and 2, and the virus was successfully isolated in several org...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760170208

    authors: Rasinhas ADC,Silva MAND,Caldas GC,Jácome FC,Leonardo R,Santos FBD,Nunes PCG,Barth OM,Barreto-Vieira DF

    更新日期:2018-02-05 00:00:00

  • Role of cytokines in the formation and downregulation of hepatic circumoval granulomas and hepatic fibrosis in Schistosoma mansoni-infected mice.

    abstract::Schistosoma mansoni infections are associated with a strong Th2 cytokine response. Treatment of mice with IL-12 or anti-IL-2 or anti-IL-4 before i.v. injection of eggs increased IFN-gamma production and downregulated Th2 responses and pulmonary granuloma size. Conversely, anti-IFN-gamma antibody treatment increased Th...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000700004

    authors: Cheever AW,Jankovic D,Yap GS,Kullberg MC,Sher A,Wynn TA

    更新日期:1998-01-01 00:00:00

  • Polysaccharide-rich fraction of Agaricus brasiliensis enhances the candidacidal activity of murine macrophages.

    abstract::A polysaccharide-rich fraction (ATF) of medicinal mushroom Agaricus brasiliensis was evaluated on the candidacidal activity, H2O2 and nitric oxide (NO) production, and expression of mannose receptors by murine peritoneal macrophages. Mice received three intraperitoneal (i.p.) injections of ATF and after 48 h their per...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762008005000011

    authors: Martins PR,Gameiro MC,Castoldi L,Romagnoli GG,Lopes FC,Pinto AV,Loyola W,Kaneno R

    更新日期:2008-05-01 00:00:00

  • Malpighian tubule polytene chromosomes of Culex quinquefasciatus (Diptera, Culicinae).

    abstract::Dipteran polytene chromosomes provide an excellent model for understanding in species complexes, as well as for structural and functional cytogenetics. The status of species in the Culex pipiens complex is controversial and the use of polytene chromosomes for cytogenetic analysis in the subfamily Culicinae has been di...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000300016

    authors: Campos J,Andrade CF,Recco-Pimentel SM

    更新日期:2003-04-01 00:00:00

  • Variation in Aedes aegypti (Diptera: Culicidae) container productivity in a slum and a suburban district of Rio de Janeiro during dry and wet seasons.

    abstract::Seasonal variation in container productivity and infestation levels by Aedes aegypti were evaluated in two areas with distinct levels of urbanization degrees in Rio de Janeiro, a slum and a suburban neighborhood. The four most productive containers can generate up to 90% of total pupae. Large and open-mouthed containe...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000056

    authors: Maciel-de-Freitas R,Marques WA,Peres RC,Cunha SP,de Oliveira RL

    更新日期:2007-06-01 00:00:00

  • Experimental American trypanomiasis in rats: sympathetic denervation, parasitism and inflammatory process.

    abstract::Tissue parasitism, inflammatory process (histologic methods) and sympathetic denervation (glyoxylic acid-induced histofluorescence for demonstration of catecholamines) were studied in the heart (atrium and ventricle) and the submandibular gland of rats infected with the Y strain of Trypanosoma cruzi. In the heart para...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761989000400013

    authors: Machado CR,Ribeiro AL

    更新日期:1989-10-01 00:00:00

  • Two new Culicoides of the paraensis species group (Diptera: Ceratopogonidae) from the Amazonian region of Peru.

    abstract::Two new species of the Culicoides paraensis species group, C. diversus Felippe-Bauer and C. peruvianus Felippe-Bauer, are described and illustrated based on female specimens from Amazonian region of Peru. A systematic key, table with numerical characters of females, and distribution of species of the C. paraensis grou...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000800014

    authors: Felippe-Bauer ML,Cáceres AG,Silva CS,Valderrama-Bazan W,Gonzales-Perez A

    更新日期:2003-12-01 00:00:00

  • Ecology of cutaneous leishmaniasis in Sinai: linking parasites, vectors and hosts.

    abstract::Cutaneous leishmaniasis (CL) is a neglected clinical form of public health importance that is quite prevalent in the northern and eastern parts of Egypt. A comprehensive study over seven years (January 2005-December 2011) was conducted to track CL transmission with respect to both sandfly vectors and animal reservoirs...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276130426

    authors: Samy AM,Doha SA,Kenawy MA

    更新日期:2014-06-01 00:00:00

  • Outstanding insecurities concerning the use of an Ov16-based ELISA in the Amazonia onchocerciasis focus.

    abstract::In a recent issue of Memórias do Instituto Oswaldo Cruz, published in Rio de Janeiro in February 2014 (109: 87-92), Adami et al. have published a survey reporting Mansonella parasite prevalence in the Amazon Region. This report makes a useful contribution to the existing knowledge of filarial parasite distribution wit...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276140079

    authors: Luz SL,Crainey JL,Shelley AJ,Rubio M

    更新日期:2014-07-01 00:00:00

  • Natural environmental water sources in endemic regions of northeastern Brazil are potential reservoirs of viable Mycobacterium leprae.

    abstract:BACKGROUND:The detection of live Mycobacterium leprae in soil and animals other than humans suggests that the environment plays a role in the transmission of leprosy. OBJECTIVE:The objective of this study was to investigate the presence of viable M. leprae in natural water sources used by the local population in five ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760170117

    authors: Arraes MLBM,Holanda MV,Lima LNGC,Sabadia JAB,Duarte CR,Almeida RLF,Kendall C,Kerr LRS,Frota CC

    更新日期:2017-12-01 00:00:00

  • Diagnostic challenges of single plaque-like lesion paucibacillary leprosy.

    abstract::The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276140212

    authors: Barbieri RR,Sales AM,Illarramendi X,Moraes MO,Nery JA,Moreira SJ,Sarno EN,Machado Ade M,Bozza FA

    更新日期:2014-11-01 00:00:00

  • Helminth parasites in six species of shorebirds (Charadrii) from the coast of Belize.

    abstract::Thirteen species of helminth parasites were recovered from six species of charadriid shorebirds (Aves: Charadriiformes) from Belize: the ruddy turnstone, Arenaria interpres, the snowy plover, Charadrius alexandrinus, the semipalmated plover, C. semipalmatus, the killdeer, C. vociferus, the white-rumped sandpiper, Cali...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000600015

    authors: Canaris AG,Kinsella JM

    更新日期:2001-08-01 00:00:00

  • Studies on survival, biological activities and behavior of Biomphalaria glabrata, the host snail of schistosomiasis, submitted to increased hydrostatic pressure: a technique.

    abstract::To study changes in survival, in biological activities and behavior of planorbids submitted to increased hydrostatic pressure, we developed a technique using two transparent chambers and a hydraulic piston. The apparatus permitted renewal of the liquid medium without substantial variations in pressure, thus eliminatin...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761988000100008

    authors: Jurberg P,Soares MS,Mascitelli AC,Favre TC,Barbosa JV

    更新日期:1988-01-01 00:00:00

  • Experimental infection of Calomys callosus (Rodentia, Cricetidae) by Toxoplasma gondii.

    abstract::Calomys callosus, Rengger 1830 (Rodentia, Cricetidae), a wild rodent found in Central Brazil, was studied to investigate its susceptibility to Toxoplasma gondii experimental infection and its humoral immune response against this protozoa. The electrophoretic profile of the serum proteins of C. callosus showed that IgG...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000100018

    authors: Favoreto-Júnior S,Ferro EA,Clemente D,Silva DA,Mineo JR

    更新日期:1998-01-01 00:00:00

  • Application of WHO International Biological Reference Standards to evaluate commercial serological tests for chronic Chagas disease.

    abstract::BACKGROUND Chagas disease, resulting from Trypanosoma cruzi infections, continues to be a health concern mainly in Latin American countries where the parasite is endemic. The laboratory diagnosis of a chronic infection is determined through serological assays for antibodies against T. cruzi and several tests are avail...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760200214

    authors: Sáez-Alquezar A,Junqueira ACV,Durans ADM,Guimarães AV,Corrêa JA,Provance DW Jr,Cabello PH,Coura JR,Viñas PA

    更新日期:2020-01-01 00:00:00

  • In vitro susceptibility of Plasmodium falciparum Welch field isolates to infusions prepared from Artemisia annua L. cultivated in the Brazilian Amazon.

    abstract::Artemisinin is the active antimalarial compound obtained from the leaves of Artemisia annua L. Artemisinin, and its semi-synthetic derivatives, are the main drugs used to treat multi-drug-resistant Plasmodium falciparum (one of the human malaria parasite species). The in vitro susceptibility of P. falciparum K1 and 3d...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000700004

    authors: Silva LF,Magalhães PM,Costa MR,Alecrim Md,Chaves FC,Hidalgo Ade F,Pohlit AM,Vieira PP

    更新日期:2012-11-01 00:00:00

  • Symbiont modulates expression of specific gene categories in Angomonas deanei.

    abstract::Trypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypa...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160228

    authors: Penha LL,Hoffmann L,Souza SS,Martins AC,Bottaro T,Prosdocimi F,Faffe DS,Motta MC,Ürményi TP,Silva R

    更新日期:2016-11-01 00:00:00

  • Association of NR1I2 gene polymorphisms and time of progression to AIDS.

    abstract:BACKGROUND:The time of progression towards AIDS can vary greatly among seropositive patients, and may be associated with host genetic variation. The NR1I2 (PXR) gene, a ligand-activated transcription factor, regulates the transcription immune pathway genes and can therefore be targets of viral replication mechanisms in...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160382

    authors: Medeiros RM,Menti CF,Benelli JL,Matte MC,Melo MG,Almeida SE,Fiegenbaum M

    更新日期:2017-04-01 00:00:00

  • Mapping the global geographic potential of Zika virus spread.

    abstract::The Americas are presently experiencing the most serious known outbreak of Zika virus (ZIKV). Here, we present a novel set of analyses using environmental characteristics, vector mosquito distributions, and socioeconomic risk factors to develop the first map to detail global ZIKV transmission risk in multiple dimensio...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160149

    authors: Samy AM,Thomas SM,Wahed AA,Cohoon KP,Peterson AT

    更新日期:2016-09-01 00:00:00

  • A comparative study of congenital toxoplasmosis between public and private hospitals from Uberlândia, MG, Brazil.

    abstract::The main purpose of the present study was to examine if there is difference in terms of incidence rates of congenital toxoplasmosis among populations assisted in public and private hospitals from Uberlândia, state of Minas Gerais, Brazil. A total of 805 serum samples from cord blood were collected, being 500 from publ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762004000100002

    authors: Segundo GR,Silva DA,Mineo JR,Ferreira MS

    更新日期:2004-02-01 00:00:00

  • Differential regulation of granuloma size and hepatic fibrosis in schistosome infections.

    abstract::Granuloma size is the variable most frequently used to evaluate the immunopathogenesis of schistosome infections. However, hepatic fibrosis is at the least an equally relevant variable. Hepatic fibrosis and the size of circumoval granulomas are frequently dissociated in experimental murine Schistosoma mansoni and S. j...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761997000500024

    authors: Cheever AW

    更新日期:1997-09-01 00:00:00

  • The Pf332 gene codes for a megadalton protein of Plasmodium falciparum asexual blood stages.

    abstract::We characterized the Plasmodium falciparum antigen 332 (Ag332) which is specifically expressed during the asexual intraerythrocytic cycle of the parasite. The corresponding Pf332 gene has been located in the subtelomeric region of chromosome 11. Furthermore, it is present in all strains so far analyzed and shows marke...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761992000700026

    authors: Mattei D,Scherf A

    更新日期:1992-01-01 00:00:00

  • Urban Chagas disease in children and women in primary care centres in Buenos Aires, Argentina.

    abstract::The primary objective of this study was to estimate the prevalence of this disease in women of childbearing age and children treated at health centres in underserviced areas of the city of Buenos Aires. Demographic and Chagas disease status data were collected. Samples for Chagas disease serology were obtained on filt...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,多中心研究

    doi:10.1590/0074-02760150107

    authors: Moscatelli G,Berenstein A,Tarlovsky A,Siniawski S,Biancardi M,Ballering G,Moroni S,Schwarcz M,Hernández S,García-Bournissen F,Cozzi AE,Freilij H,Altcheh J

    更新日期:2015-08-01 00:00:00

  • A chromosome 9 deletion in Plasmodium falciparum results in loss of cytoadherence.

    abstract::Many lines of Plasmodium falciparum undergo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and the selected cells produce gametocy...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761992000700011

    authors: Kemp DJ,Thompson J,Barnes DA,Triglia T,Karamalis F,Petersen C,Brown GV,Day KP

    更新日期:1992-01-01 00:00:00

  • Benzonidazole levels in blood vary with age in rats.

    abstract::Benznidazole (Bz) exhibits toxic side effects in animal studies and clinical use. Reductive metabolism of Bz in liver microsomes modulates the duration of its chemotherapeutic effect and its toxicity. The rate of this metabolism depends on age and is less intense in newborns and youngsters than in adults. In the prese...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000300021

    authors: Bulffer RF,Castro JA,Fanelli SL

    更新日期:2011-05-01 00:00:00

  • Descriptions of Lutzomyia (Evandromyia) georgii n. sp. and a synopsis of the series infraspinosa (Diptera: Psychodidae).

    abstract::Lutzomyia georgii n. sp. and the female of L. tarapacaensis in the Series infraspinosa of the subgenus Evandromyia are described, from specimens collected in rainforest in the north of the State of Pará, Brazil. The new species was taken together with five other Evandromyia species including L. infraspinosa (sensu str...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000200017

    authors: Freitas RA,Barrett TV

    更新日期:2002-03-01 00:00:00

  • Emergence of Trichosporon mycotoxinivorans (Apiotrichum mycotoxinivorans) invasive infections in Latin America.

    abstract::We report the first two cases of Trichosporon mycotoxinivorans infections in Latin America. We also conducted a literature review and a microbiological investigation, including that of clinical and environmental isolates. A 30-year-old man with chronic renal failure had disseminated infection after dialysis and a 15-y...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760170011

    authors: Almeida JN Jr,Francisco EC,Barberino MGMA,Silva LVRFD Filho,Brandão OM,Colombo AL,Padovan ACB

    更新日期:2017-10-01 00:00:00

  • Occurence of larval Culicidae (Diptera) in water retained in Aquascypha hydrophora (Fungus: Stereaceae) in Central Amazônia, Brazil.

    abstract::The community structure of insects, especially mosquito larvae, in water held in the fungus Aquascypha hydrophora (Berk.) Reid (Stereaceae) is reported. The study was done in the Adolpho Ducke Forest Reserve, 26 km east of Manaus, AM, Brazil, from September 1998 through November 1999. The most abundant entomofauna wer...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000800023

    authors: Ferreira RL,Oliveira AF,Pereira ES,Hamada N

    更新日期:2001-11-01 00:00:00

  • The zinc finger protein TcZFP2 binds target mRNAs enriched during Trypanosoma cruzi metacyclogenesis.

    abstract::Trypanosomes are parasitic protozoa in which gene expression is primarily controlled through the regulation of mRNA stability and translation. This post-transcriptional control is mediated by various families of RNA-binding proteins, including those with zinc finger CCCH motifs. CCCH zinc finger proteins have been sho...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000600014

    authors: Mörking PA,Rampazzo Rde C,Walrad P,Probst CM,Soares MJ,Gradia DF,Pavoni DP,Krieger MA,Matthews K,Goldenberg S,Fragoso SP,Dallagiovanna B

    更新日期:2012-09-01 00:00:00