A chromosome 9 deletion in Plasmodium falciparum results in loss of cytoadherence.

Abstract:

:Many lines of Plasmodium falciparum undergo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and the selected cells produce gametocytes. The deletion also results in loss of expression of PfEMP1, the putative cytoadherence ligand, suggesting that PfEMP1 or a regulatory gene controlling PfEMP1 expression and gametocytogenesis may be encoded in this region. We have isolated several markers for the deleted region and are currently using a YAC-P. falciparum library to investigate this region of the genome in detail.

journal_name

Mem Inst Oswaldo Cruz

authors

Kemp DJ,Thompson J,Barnes DA,Triglia T,Karamalis F,Petersen C,Brown GV,Day KP

doi

10.1590/s0074-02761992000700011

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

85-9

eissn

0074-0276

issn

1678-8060

pii

S0074-02761992000700011

journal_volume

87 Suppl 3

pub_type

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