Commercial drugs containing flavonoids are active in mice with malaria and in vitro against chloroquine-resistant Plasmodium falciparum.


:BACKGROUND The main strategy to control human malaria still relies on specific drug treatment, limited now by Plasmodium falciparum-resistant parasites, including that against artemisinin derivatives. Despite the large number of active compounds described in the literature, few of them reached full development against human malaria. Drug repositioning is a fast and less expensive strategy for antimalarial drug discovery, because these compounds are already approved for human use. OBJECTIVES To identify new antimalarial drugs from compounds commercially available and used for other indications. METHODS Accuvit®, Ginkgo® and Soyfit®, rich in flavonoids, and also the standard flavonoids, hesperidin, quercetin, and genistein were tested against blood cultures of chloroquine-resistant P. falciparum, as well as chloroquine, a reference antimalarial. Inhibition of parasite growth was measured in immunoenzymatic assay with monoclonal anti-P. falciparum antibodies, specific to the histidine-rich protein II. Tests in mice with P. berghei malaria were based on percent of parasitaemia reduction. These compounds were also evaluated for in vitro cytotoxicity. FINDINGS The inhibition of parasite growth in vitro showed that Accuvit® was the most active drug (IC50 5 ± 3.9 μg/mL). Soyfit® was partially active (IC50 13.6 ± 7.7 μg/mL), and Ginkgo® (IC50 38.4 ± 14 μg/mL) was inactive. All such compounds were active in vivo at a dose of 50 mg/kg body weight. Accuvit® and quercetin induced the highest reduction of P. berghei parasitaemia (63% and 53%, respectively) on day 5 after parasite inoculation. As expected, the compounds tested were not toxic. MAIN CONCLUSIONS The antimalarial activity of Accuvit® was not related to flavonoids only, and it possibly results from synergisms with other compounds present in this drug product, such as multivitamins. Multivitamins in Accuvit® may explain its effect against the malaria parasites. This work demonstrated for the first time the activity of these drugs, which are already marketed.


Mem Inst Oswaldo Cruz


Penna-Coutinho J,Aguiar AC,Krettli\/ AU




Has Abstract


2018-12-06 00:00:00














  • Ultrastructure of endogenous stages of Eimeria ninakohlyakimovae Yakimoff & Rastegaieff, 1930 Emend. Levine, 1961 in experimentally infected goat.

    abstract::The ultrastructure of endogenous stages of Eimeria ninakohlyakimovae was observed in epithelial cells of cecum and colon crypts from a goat experimentally infected with 2.0 x 10(5) oocysts/kg. The secondary meronts developed above the nucleus of the host cell. The nucleus first divides and merozoites then form on the ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Vieira LS,Lima JD,Ribeiro MF,Bozzi IA,Camargos ER

    更新日期:1997-07-01 00:00:00

  • DNA evidence of Trypanosoma cruzi in the Chilean wild vector Mepraia spinolai (Hemiptera: Reduviidae).

    abstract::Molecular evidence showed 46.2% of Trypanosoma cruzi infection in Mepraia spinolai insects from North-Central Chile, which is significantly higher than previous reports of up to 26% by microscopic observation. Our results show similar infection levels among nymphal stages, ranging from 38.3 to 54.1%, indicating that y...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Botto-Mahan C,Ortiz S,Rozas M,Cattan PE,Solari A

    更新日期:2005-05-01 00:00:00

  • The finding of eggs of Diphyllobothrium in human coprolites (4,100-1,950 B.C.) from northern Chile.

    abstract::Twenty six coprolites from an archaeological site in the province of Iquique, northern Chile, were examined for parasites. Coprolites were found in two excavation units, I and II (Tiliviche site), dated respectively at 5,900 B.C. to 4,110 B.C. and 4,110 B.C. to 1,950 B.C., and identified as of human origin. Only at th...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 历史文章,杂志文章


    authors: Ferreira LF,de Araújo AJ,Confalonieri UE,Nuñez L

    更新日期:1984-04-01 00:00:00

  • Deep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata.

    abstract:BACKGROUND:Biomphalaria glabrata snails are widely distributed in schistosomiasis endemic areas like America and Caribe, displaying high susceptibility to infection by Schistosoma mansoni. After the availability of B. glabrata genome and transcriptome data, studies focusing on genetic markers and small non-coding RNAs ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Queiroz FR,Portilho LG,Jeremias WJ,Babá ÉH,do Amaral LR,Silva LM,Coelho PMZ,Caldeira RL,Gomes MS

    更新日期:2020-07-01 00:00:00

  • Ultrasound monitoring of structural urinary tract disease in Schistosoma haematobium infection.

    abstract::A major advance in our understanding of the natural history of Schistosoma haematobium-related morbidity has come through the introduction of the portable ultrasound machines for non-invasive examination of the kidneys and bladder. With the use of generators or battery packs to supply power in non-clinical field setti...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: King CH

    更新日期:2002-01-01 00:00:00

  • Toxocara canis and the allergic process.

    abstract::The protective effect of infectious agents against allergic reactions has been thoroughly investigated. Current studies have demonstrated the ability of some helminths to modulate the immune response of infected hosts. The objective of the present study was to investigate the relationship between Toxocara canis infect...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Zaia MG,Oliveira SR,Castro CA,Soares EG,Afonso A,Monnazzi LG,Peitl Filho O,Faccioli LH,Anibal Fde F

    更新日期:2015-09-01 00:00:00

  • Vascular dysfunction as a target for adjuvant therapy in cerebral malaria.

    abstract::Cerebral malaria (CM) is a life-threatening complication of Plasmodium falciparum malaria that continues to be a major global health problem. Brain vascular dysfunction is a main factor underlying the pathogenesis of CM and can be a target for the development of adjuvant therapies for the disease. Vascular occlusion b...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审


    authors: Carvalho LJ,Moreira Ada S,Daniel-Ribeiro CT,Martins YC

    更新日期:2014-08-01 00:00:00

  • Leishmania (Viannia) naiffi: rare enough to be neglected?

    abstract::In the Brazilian Amazon, American tegumentary leishmaniasis (ATL) is endemic and presents a wide spectrum of clinical manifestations due, in part, to the circulation of at least seven Leishmania species. Few reports of Leishmania (Viannia) naiffi infection suggest that its occurrence is uncommon and the reported cases...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Fagundes-Silva GA,Romero GA,Cupolillo E,Yamashita EP,Gomes-Silva A,Guerra JA,Da-Cruz AM

    更新日期:2015-09-01 00:00:00

  • Molecular approaches for eco-epidemiological studies of Paracoccidioides brasiliensis.

    abstract::Medical mycology has greatly benefited from the introduction of molecular techniques. New knowledge on molecular genetics has provided both theoretical and practical frameworks, permitting important advances in our understanding of several aspects of pathogenic fungi. Considering Paracoccidioides brasiliensis in parti...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审


    authors: Richini-Pereira VB,Bosco Sde M,Theodoro RC,Macoris SA,Bagagli E

    更新日期:2009-07-01 00:00:00

  • The rational search for natural neolignans.

    abstract::A rational method of search for natural neolignans of desired structures is outlined. This involves consultation of a collection of chemical profiles of plant families. The profiles are assembled considering the biosynthetic class (in the present case lignoids), subclass (neolignans), structural types (neolignan skele...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审


    authors: Gottlieb OR

    更新日期:1991-01-01 00:00:00

  • Impairment of cell division of Trypanosoma cruzi epimastigotes.

    abstract::The mechanisms that facilitate the adaptation of Trypanosoma cruzi to two distinct hosts, insect and vertebrate, are poorly understood, in part due to the limited ability to perform gene disruption studies by homologous recombination. This report describes a developmentally-defective phenotype that resulted from integ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Zacks MA

    更新日期:2007-02-01 00:00:00

  • Functional complementation of a yeast knockout strain by Schistosoma mansoni Rho1 GTPase in the presence of caffeine, an agent that affects mutants defective in the protein kinase C signal transduction pathway.

    abstract::In a previous study, the Schistosoma mansoni Rho1 protein was able to complement Rho1 null mutant Saccharomyces cerevisiae cells at restrictive temperatures and under osmotic stress (low calcium concentration) better than the human homologue (RhoA). It is known that under osmotic stress, the S. cerevisiae Rho1 trigger...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Aguiar PH,Santos DN,Lobo FP,Santos TM,Macedo AM,Pena SD,Machado CR,Franco GR

    更新日期:2006-09-01 00:00:00

  • Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages.

    abstract::Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expressio...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Villela AD,Rodrigues-Junior VS,Pinto AFM,Falcão VCA,Sánchez-Quitian ZA,Eichler P,Bizarro CV,Basso LA,Santos DS

    更新日期:2017-11-01 00:00:00

  • Seroprevalence of HBV, HCV and HIV co-infection in selected individuals from state of São Paulo, Brazil.

    abstract::Few studies are available on hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV) co-infection in populations living in small and medium-sized Brazilian cities. We evaluated the seroprevalence of these viruses in selected individuals from a clinic of infectology, who were referred to...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Portelinha Filho AM,Nascimento CU,Tannouri TN,Troiani C,Ascêncio EL,Bonfim R,D'Andrea LA,Prestes-Carneiro LE

    更新日期:2009-11-01 00:00:00

  • Regulatory volume decrease in Leishmania mexicana: effect of anti-microtubule drugs.

    abstract::The trypanosomatid cytoskeleton is responsible for the parasite's shape and it is modulated throughout the different stages of the parasite's life cycle. When parasites are exposed to media with reduced osmolarity, they initially swell, but subsequently undergo compensatory shrinking referred to as regulatory volume d...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Dagger F,Valdivieso E,Marcano AK,Ayesta C

    更新日期:2013-02-01 00:00:00

  • The first report of Aedes (Stegomyia) albopictus in Haiti.

    abstract::Aedes albopictus was found in six of the 10 departments of Haiti and in 14 of the 35 communes surveyed. The survey found the larvae of Ae. albopictus in 13 different types of containers. Used tires and tins were by far the most common breeding sites used by this mosquito species. At the breeding sites, Ae. albopictus ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Fernández Mdel C,Jean YS,Callaba CA,López LS

    更新日期:2012-03-01 00:00:00

  • Appraisal of Phlebotomus argentipes habitat suitability using a remotely sensed index in the kala-azar endemic focus of Bihar, India.

    abstract::Visceral leishmaniasis, or kala-azar, is recognised as a serious emerging public health problem in India. In this study, environmental parameters, such as land surface temperature (LST) and renormalised difference vegetation indices (RDVI), were used to delineate the association between environmental variables and Phl...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Kesari S,Bhunia GS,Chatterjee N,Kumar V,Mandal R,Das P

    更新日期:2013-04-01 00:00:00

  • Reliability of current estimates of schistosomiasis prevalence in the Rainforest Zone of the state of Pernambuco, Northeastern Brazil.

    abstract::The Program for Schistosomiasis Control within the Unified Health System (PCE-SUS) was implemented by 1999 in the Rainforest Zone or "Zona da Mata" of Pernambuco (ZMP) aiming to carry out biennial stool surveys of whole populations through municipal health organs followed by treatment of the positives through the loca...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Favre TC,Ximenes RA,Galvão AF,Pereira AP,Wanderlei TN,Barbosa CS,Pieri OS

    更新日期:2006-09-01 00:00:00

  • Isotype specific immune responses in murine experimental toxocariasis.

    abstract::In this work, a murine experimental model of toxocariasis has been developed in BALB/c, C57BL/10 and C3H murine strains orally inoculated with 4,000 Toxocara canis embryonated eggs, in order to investigate the isotype-specific immune responses against excretory-secretory antigens from larvae. T. canis specific IgG+M, ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Cuéllar C,Fenoy S,del Aguila C,Guillén JL

    更新日期:2001-05-01 00:00:00

  • Human immunodeficiency virus type 1 neutralization by plasma from B or F genotype infected individuals.

    abstract::Anti-human immunodeficiency virus type 1 (HIV-1) "binding antibodies" (antibodies capable of binding to synthetic peptides or proteins) occur throughout HIV-1 infection, are high-titered and highly cross-reactive, as confirmed in this study by analyzing plasma from B and F genotype HIV-1 infected individuals. Plasma f...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Bongertz V,Teixeira SL,Grinztejn B,Pilotto JH,Veloso VG,Morgado MG,Bastos FI,Ouverney EP

    更新日期:2005-02-01 00:00:00

  • Acute Chagas disease in El Salvador 2000-2012 - need for surveillance and control.

    abstract::Several parasitological studies carried out in El Salvador between 2000-2012 showed a higher frequency of acute cases of Chagas disease than that in other Central American countries. There is an urgent need for improved Chagas disease surveillance and vector control programs in the provinces where acute Chagas disease...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Sasagawa E,Aguilar AV,Ramírez MA,Chévez JE,Nakagawa J,Cedillos RA,Kita K

    更新日期:2014-04-01 00:00:00

  • Adult and nymphs of Microtriatoma trinidadensis (Lent, 1951) (Hemiptera: Reduviidae) caught from peridomestic environment in Bolivia.

    abstract::This is the first report of adult and nymphs (20 nymphs of all stages and 4 adults) of Microtriatoma trinidadensis (Lent 1951) (Hemiptera: Reduviidae: Triatominae) collected in peridomestic environment, in the department of La Paz, Bolivia. These specimens were associated to Rhodnius stali Lent, Jurberg & Galvão 1993....

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: De la Riva J,Matias A,Torrez M,Martínez E,Dujardin JP

    更新日期:2001-10-01 00:00:00

  • Larval dispersal and predation in experimental populations of Chrysomya albiceps and Cochliomyia macellaria (Diptera: Calliphoridae).

    abstract::In this study we investigated the larval dispersal associated with larval predation in experimental populations of Chrysomya albiceps and Cochliomyia macellaria. Frequency distribution of sampling units (G test) in the substrate was used to evaluate variation in larval dispersal. An experimental acrylic channel (1 x 0...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: de Andrade JB,Rocha FA,Rodrigues P,Rosa GS,Faria Ldel B,Von Zuben CJ,Rossi MN,Godoy WA

    更新日期:2002-12-01 00:00:00

  • Larval recovery of Toxocara cati in experimentally infected Rattus norvegicus and analysis of the rat as potential reservoir for this ascarid.

    abstract::Toxocara cati is a common feline parasite transmitted by the ingestion of embryonated eggs, by the transmammary route or by predation of paratenic hosts harbouring third-stage larvae in their bodies. In the present study, the larval distribution of T. cati in tissues and organs of Rattus norvegicus experimentally infe...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Santos SV,Lescano SZ,Castro JM,Chieffi PP

    更新日期:2009-09-01 00:00:00

  • Susceptibility to diazinon in populations of the horn fly, Haematobia irritans (Diptera: Muscidae), in Central Brazil.

    abstract::From October 2000 to April 2001, insecticide bioassays were conducted in 18 ranches from 10 counties in the states of Mato Grosso and Mato Grosso do Sul, in Central Brazil. Horn flies from wild populations were exposed to diazinon-impregnated filter papers immediately after collection on cattle, and mortality was reco...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Barros AT,Gomes A,Ismael AP,Koller WW

    更新日期:2002-09-01 00:00:00

  • Aspects of classification of Hemiptera hemocytes from six triatomine species.

    abstract::The objective of this work was to characterize, and compare different morphological types of hemocytes of Rhodnius prolixus, Rhodnius robustus, Rhodnius neglectus, Triatoma infestans, Panstrongylus megistus, and Dipetalogaster maximus. This information provides the basis for studying the cellular immune systems of the...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: de Azambuja P,Garcia ES,Ratcliffe NA

    更新日期:1991-01-01 00:00:00

  • Acute Chagas disease in the state of Pará, Amazon Region: is it increasing?

    abstract::Acute Chagas disease (ACD) has a distinct epidemiological profile in the Amazon Region, with cases and outbreaks of Trypanosoma cruzi infection being possibly related to the ingestion of contaminated food. Data on ACD in the state of Pará retrieved from 2000 to 2016 from the Brazilian Notifiable Diseases Information S...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Santos VRCD,Meis J,Savino W,Andrade JAA,Vieira JRDS,Coura JR,Junqueira ACV

    更新日期:2018-01-01 00:00:00

  • Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures.

    abstract::Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28 degrees C and 37 degrees C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Several proteins with molecular weights ranging f...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Abath FG,de Almeida AM,Ferreira LC

    更新日期:1990-01-01 00:00:00

  • TcruziDB, an integrated database, and the WWW information server for the Trypanosoma cruzi genome project.

    abstract::Data analysis, presentation and distribution is of utmost importance to a genome project. A public domain software, ACeDB, has been chosen as the common basis for parasite genome databases, and a first release of TcruziDB, the Trypanosoma cruzi genome database, is available by ftp from

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Degrave W,de Miranda AB,Amorim A,Brandão A,Aslett M,Vandeyar M

    更新日期:1997-11-01 00:00:00

  • The outcome of acute schistosomiasis infection in adult mice with postnatal exposure to maternal malnutrition.

    abstract::Maternal malnutrition during the lactation period in early development may have long-term programming effects on adult offspring. We evaluated the combined effects of parasitological behaviour and histopathological features and malnutrition during lactation. Lactating mice and their pups were divided into a control gr...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Corrêa CL,Lisboa PC,Oliveira Ed,Moura EG,Oliveira RM,Gomes AC,Machado-Silva JR

    更新日期:2011-08-01 00:00:00