Symbiont modulates expression of specific gene categories in Angomonas deanei.

Abstract:

:Trypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypanosomatid. In this paper, we sought to investigate how symbionts influence host cells by characterising and comparing the transcriptomes of the symbiont-containing A. deanei (wild type) and the symbiont-free aposymbiotic strains. The comparison revealed that the presence of the symbiont modulates several differentially expressed genes. Empirical analysis of differential gene expression showed that 216 of the 7625 modulated genes were significantly changed. Finally, gene set enrichment analysis revealed that the largest categories of genes that downregulated in the absence of the symbiont were those involved in oxidation-reduction process, ATP hydrolysis coupled proton transport and glycolysis. In contrast, among the upregulated gene categories were those involved in proteolysis, microtubule-based movement, and cellular metabolic process. Our results provide valuable information for dissecting the mechanism of endosymbiosis in A. deanei.

journal_name

Mem Inst Oswaldo Cruz

authors

Penha LL,Hoffmann L,Souza SS,Martins AC,Bottaro T,Prosdocimi F,Faffe DS,Motta MC,Ürményi TP,Silva R

doi

10.1590/0074-02760160228

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

686-691

issue

11

eissn

0074-0276

issn

1678-8060

pii

S0074-02762016005019105

journal_volume

111

pub_type

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