Evaluation of a monoclonal antibody affinity purified antigen for zymodeme specific serological diagnosis of Trypanosoma cruzi infection.

Abstract:

:Theoretically, serological assays with affinity purified marker antigens can allow strain-specific diagnosis even when parasites cannot be retrieved from an infected host. A Trypanosoma cruzi antigen was purified by affinity chromatography using a zymodeme (Z) 2 specific monoclonal antibody (2E2C11). An indirect enzyme-linked immunosorbent assay (ELISA) based on the purified antigen could discriminate between sera from rabbits immunized with T. cruzi zymodeme clones but could not discriminate between sera from mice infected with different zymodemes.

journal_name

Mem Inst Oswaldo Cruz

authors

Schechter M

doi

10.1590/s0074-02761987000100014

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

81-5

issue

1

eissn

0074-0276

issn

1678-8060

pii

S0074-02761987000100014

journal_volume

82

pub_type

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