Improved reverse transcription-polymerase chain reaction assay for the detection of flaviviruses with semi-nested primers for discrimination between dengue virus serotypes and Zika virus.

abstract：:The number of visceral leishmaniasis (VL) cases has increased over the past 10 years in Brazil, especially in the North and Northeast regions of the country. The aim of this study was to evaluate the urbanisation of VL vectors in Barcarena, Pará, an area in northern Brazil where VL is endemic. Sandflies were captured ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Oliveira DM,Saraiva EM,Ishikawa EA,Sousa AA,Silva EO,Silva IM

更新日期：2011-12-01 00:00:00

abstract：:For the first time Angiostrongylus vasorum in Canis familiaris in Minas Gerais State, Brazil, is described. The description and measurements of three males and ten females are presented together with a diagram of the parasite. ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： dos Santos Lima W,de Araujo Costa HM,Guimarães MP,Leite AC

更新日期：1985-04-01 00:00:00

abstract：:Nitroarylidenemalononitriles and their cyanoacetamide derivatives with remarkable anti-epimastigote properties, were synthesized attempting to obtain new 3,5-diamino-4-(5'-nitroarylidene)-4H-thiadiazine 1,1-dioxide derivatives, which in previous reports had shown anti-Trypanosoma cruzi activity. Tests to evaluate the ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Muelas-Serrano S,Le-Senne A,Fernandez-Portillo C,Nogal JJ,Ochoa C,Gomez-Barrio A

更新日期：2002-06-01 00:00:00

abstract：:This study aimed at identifying the best ovitrap installation sites for gravid Aedes aegypti in Mirassol, state of São Paulo, Brazil. Ovitraps were installed in ten houses per block over ten blocks. Four ovitraps were placed per residence, one in the bedroom, one in the living room, and two outdoors with one in a shel...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Dibo MR,Chiaravalloti-Neto F,Battigaglia M,Mondini A,Favaro EA,Barbosa AA,Glasser CM

更新日期：2005-07-01 00:00:00

abstract：:There is no effective chemotherapy against diseases caused by Phytomonas sp., a plant trypanosomatid responsible for economic losses in major crops. We tested three triazolo-pyrimidine complexes [two with Pt(II), and another with Ru(III)] against promastigotes of Phytomonas sp. isolated from Euphorbia characias. The i...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Magán R,Marín C,Salas JM,Barrera-Pérez M,Rosales MJ,Sánchez-Moreno M

更新日期：2004-10-01 00:00:00

abstract：:Citral, the main constituent of lemongrass (Cymbopogon citratus) essential oil, was added to Trypanosoma cruzi cultures grown in TAU3AAG medium to observe the effect on the epimastigote-to-trypomastigote differentiation process (metacyclogenesis). Our results showed that citral (20 μg/mL) did not affect epimastigote v...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Cardoso J,Soares MJ

更新日期：2010-12-01 00:00:00

abstract：:This study aims to investigate the importance of the serum factors present in the plasma of resistant Biomphalaria tenagophila snails, when transferred to susceptible conspecific. Susceptible B. tenagophila (CF) received plasma from resistant B. tenagophila (Taim), and both were later infected with Schistosoma mansoni...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Coelho JR,Bezerra FS

更新日期：2006-02-01 00:00:00

abstract：:Details are given of the prevalence rates of onchocerciasis from the most recent surveys (1989) conducted in northern Ecuador. The disease has intensified and dispersed considerably due to migration of infected individuals and the presence of a highly efficient vector. Comparison of these data with those from two prev...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Guderian RH,Shelley AJ

更新日期：1992-07-01 00:00:00

abstract：:As pyrethroids are presently the favored group of insecticides to control triatomines, we performed a series of bioassays to determine the intrinsic activity of some of the main compounds used in the control campaigns, against five of the main species of triatomines to be controlled. Comparing the insecticides it can ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Oliveira Filho AM

更新日期：1999-01-01 00:00:00

abstract：:The yield as well as phenotypic and functional parameters of canine peripheral blood monocyte-derived macrophages were analyzed. The cells that remained adherent to Teflon after 10 days of culture had high phagocytic activity when inoculated with Leishmania chagasi. Flow cytometric analysis demonstrated that more than...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Bueno R,Mello MN,Menezes CA,Dutra WO,Santos RL

更新日期：2005-08-01 00:00:00

abstract：:Toxoplasma gondii has a very wide intermediate host range and is thought to be able to infect all warm blooded animals. The parasite causes a spectrum of different diseases and clinical symptoms within the intermediate hosts and following infection most animals develop adaptive humoral and cell-mediated immune respons...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Innes EA,Bartley PM,Maley S,Katzer F,Buxton D

更新日期：2009-03-01 00:00:00

abstract：:A major goal in the treatment of acute ischemia of a vascular territory is to restore blood flow to normal values, i.e. to "reperfuse" the ischemic vascular bed. However, reperfusion of ischemic tissues is associated with local and systemic leukocyte activation and trafficking, endothelial barrier dysfunction in postc...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Souza DG,Teixeira MM

更新日期：2005-03-01 00:00:00

abstract：:Lutzomyia (Lutzomyia) cruzi has been named as a probable vector of Leishmania chagasi in Corumbá, Mato Grosso do Sul, Brazil. Taxonomically L. cruzi is closely related to the L. longipalpis species complex. Females of L. cruzi and L. longipalpis are morphologically indistinguishable and associated males must be examin...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Brazil RP,Hamilton JG

更新日期：2002-04-01 00:00:00

abstract：:The biochemical pathways involved in nicotinamide adenine dinucleotide (NAD) biosynthesis converge at the enzymatic step catalysed by nicotinamide mononucleotide adenylyltransferase (NMNAT, EC: 2.7.7.1). The majority of NMNATs are assembled into homo-oligomeric states that comprise 2-6 subunits. Recently, the NMNAT of...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Contreras-Rodríguez LE,Marin-Mogollon CY,Sánchez-Mejía LM,Ramírez-Hernández MH

更新日期：2018-07-10 00:00:00

abstract：:Rhesus monkeys (Macaca mulatta) were infected subcutaneously with 1.0 x 10(4) to 1.5 x 10(4) metacyclic trypomastigotes of Trypanosoma cruzi (Colombian strain). Parasitological and immunological parameters were evaluated in these animals for periods of 1 month to over 3 years. A chagoma was observed between the 3rd an...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Bonecini-Almeida Mda G,Galvão-Castro B,Pessoa MH,Pirmez C,Laranja F

更新日期：1990-04-01 00:00:00

abstract：:Chagas cardiomyopathy is the most frequent and most severe manifestation of chronic Chagas disease, and is one of the leading causes of morbidity and death in Latin America. Although the pathogenesis of Chagas cardiomyopathy is incompletely understood, it may involve several mechanisms, including parasite-dependent my...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Rassi A Jr,Marin JA Neto,Rassi A

更新日期：2017-03-01 00:00:00

abstract：:Analysis of zymograms with SDS-polyacrilamide gel electrophoresis containing gelatin as substrate, and performed on samples of haemolymph or fat body taken from Rhodnius prolixus inoculated or not with Enterobacter cloacae, demonstrated distinct patterns of protease activities: (i) in the haemolymph two proteases were...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Feder D,Salles JM,Garcia ES,Azambuja P

更新日期：1998-11-01 00:00:00

abstract：:A large bibliographic survey provided data on Trypanosoma cruzi serology covering the period 1948-1984. Epidemiological-demographic methods provided an estimate of 11% for the prevalence of positive serology in Brazil, by 1984. Significant temporal trends were observed for most of the Brazilian geographical regions as...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Feitosa MF,Krieger H

更新日期：1991-04-01 00:00:00

abstract：:As in many areas of Brazil, the AIDS epidemic in Curitiba is relatively stable, but surveillance is important to support public policy. The molecular characteristics of HIV may be instrumental for monitoring epidemic trends. We evaluated plasma HIV-1 RNA (n = 37) from 38 cases presenting with positive serology, who we...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Ferreira JL,Thomaz M,Rodrigues R,Harrad D,Oliveira CM,Oliveira CA,Batista JP,Ito TS,Brigido LF

更新日期：2008-12-01 00:00:00

abstract：:Corynebacterium striatum is a potentially pathogenic microorganism with the ability to produce outbreaks of nosocomial infections. Here, we document a nosocomial outbreak caused by multidrug-resistant (MDR) C. striatum in Rio de Janeiro, Brazil. C. striatum identification was confirmed by 16S rRNA and rpoB gene sequen...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Baio PV,Mota HF,Freitas AD,Gomes DL,Ramos JN,Sant'Anna LO,Souza MC,Camello TC,Hirata Junior R,Vieira VV,Mattos-Guaraldi AL

更新日期：2013-02-01 00:00:00

abstract：:Patients with acute schistosomiasis were studied before and after oxamniquine treatment. They had been exposed to cercariae 5 to 9 weeks before, and presented compatible clinical manifestations, eosinophilia, and high levels of total IgE. Interferon-gamma (IFN-gamma) and interleukin-4 were measured by ELISA in whole b...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： de Souza JR,Morais CN,Aroucha ML,Miranda PJ,Barbosa CS,Domingues AL,Carvalho Júnior LB,Abath FG,Montenegro SM

更新日期：2007-05-01 00:00:00

abstract：:Acinetobacter baumannii is a strictly aerobic bacterium which causes severe infections, however its pathogenic characteristics are not well defined. Thirteen A. baumannii strains isolated from urine of hospitalized and nonhospitalized patients with different ages were investigated for the presence of virulence factors...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Braun G,Vidotto MC

更新日期：2004-12-01 00:00:00

abstract：:While the eosinophil's effector functions clearly can contribute to the pathogenesis of allergic diseases, the evolutionary benefit to having eosinophils as a distinct class of leukocyte is not clear, especially if one must reconsider the nominally beneficial role of eosinophils in parasite host defense, Eosinophils a...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Weller PF,Lim K

更新日期：1997-01-01 00:00:00

abstract：:In laboratory trials an aqueous solution of Piquerol A from Piqueria trinervia, collected in several regions of Mexico, showed a molluscicide action on the adults of eight different pulmonates snails species: Fossaria (Fossaria) humilis, F. (Bakerilymnaea) sp., Pseudosuccinea columella and Stagnicola attenuata from Me...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Cruz-Reyes A,Chavarin C,Campos Arias MP,Taboada J,Jimenez M

更新日期：1989-01-01 00:00:00

abstract：:Toxocara cati is a common feline parasite transmitted by the ingestion of embryonated eggs, by the transmammary route or by predation of paratenic hosts harbouring third-stage larvae in their bodies. In the present study, the larval distribution of T. cati in tissues and organs of Rattus norvegicus experimentally infe...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Santos SV,Lescano SZ,Castro JM,Chieffi PP

更新日期：2009-09-01 00:00:00

abstract：:There has been good progress in inferring the evolutionary relationships within trypanosomes from DNA data as until relatively recently, many relationships have remained rather speculative. Ongoing molecular studies have provided data that have adequately shown Trypanosoma to be monophyletic and, rather surprisingly, ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Stothard J

更新日期：2000-07-01 00:00:00

abstract：:The prevalence of atopic diseases and diabetes is increasing worldwide though the concurrence of these pathologies in individual patients is found less frequent than it would be predicted. Moreover, co-existence of diabetes and allergy is generally marked by attenuation of their respective symptoms, and effective trea...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Carvalho VF,Barreto EO,Cordeiro RS,Lagente V,Martins MA,e Silva PM

更新日期：2005-03-01 00:00:00

abstract：:A shift from large to small average sizes was observed in Triatoma infestans and Rhodnius domesticus between field and domestic (or laboratory) conditions of life. It was more pronounced in the female specimens, leading to a subsequent reduction of sexual size dimorphism. This feature is discussed in terms of genetic ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Dujardin J,Steinden M,Chavez T,Machane M,Schofield C

更新日期：1999-07-01 00:00:00

abstract：BACKGROUND:Cutaneous leishmaniasis (CL) generally presents with a single or several localised cutaneous ulcers without involvement of mucous membranes. Ulcerated lesions are susceptible to secondary contamination that may slow the healing process. OBJECTIVE:This study verified the influence of non-parasitic wound infe...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Antonio LF,Lyra MR,Saheki MN,Schubach AO,Miranda LFC,Madeira MF,Lourenço MCDS,Fagundes A,Ribeiro ÉADS,Barreto L,Pimentel MIF

更新日期：2017-09-01 00:00:00

abstract：:A simple and rapid method for differentiating the sibling species Biomphalaria tenagophila and Biomphalaria occidentalis by agarose gel electrophoresis (AGE) is described. Snail hemolymph is used as the test sample and the red coloration of the hemoglobin fraction permits visualization of the migration patterns withou...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Bailey JB,Michelson EH,Paraense WL

更新日期：1986-07-01 00:00:00