Isolation and molecular identification of Leishmania chagasi from a bat (Carollia perspicillata) in northeastern Venezuela.

Abstract:

:This report describes the isolation of a Leishmania chagasi strain from a bat (Carollia perspicillata), and its identification using biological methods and molecular characterization. The parasites were isolated in an artificial culture medium from a blood sample extracted from a bat heart. The isolate was then inoculated into the footpads of Balb/c mice, which subsequently developed a typical nodular leishmanial lesion; the parasites were confirmed as Leishmania by smear and histopathology. Molecular characterization of the parasites was performed by polymerase chain reaction with species-specific primers, kDNA restriction pattern following Hae III endonuclease digestion and dot blot hybridization using a kDNA probe. This report demonstrates that bats can be hosts for L. chagasi species and suggests the need for studies to determine whether they may be involved in foci of visceral leishmaniasis.

journal_name

Mem Inst Oswaldo Cruz

authors

De Lima H,Rodríguez N,Barrios MA,Avila A,Cañizales I,Gutiérrez S

doi

10.1590/s0074-02762008000400018

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

412-4

issue

4

eissn

0074-0276

issn

1678-8060

pii

S0074-02762008000400018

journal_volume

103

pub_type

杂志文章
  • Vertical human immunodeficiency virus type 1--HIV-1--transmission--a review.

    abstract::Several factors appear to affect vertical HIV-1 transmission, dependent mainly on characteristics of the mother (extent of immunodeficiency, co-infections, risk behaviour, nutritional status, immune response, genetical make-up), but also of the virus (phenotype, tropism) and, possibly, of the child (genetical make-up)...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02762001000100001

    authors: Bongertz V

    更新日期:2001-01-01 00:00:00

  • Determination and control of schistosomiasis.

    abstract::The subject of this conference reflects the scientific community's interest in seeking to understand the complex causal web whose various social, economic, and biological components interact in the production and reproduction of schistosomiasis and its control in relation to community participation. From the onset, th...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761995000200005

    authors: Barbosa FS

    更新日期:1995-03-01 00:00:00

  • Acoustic communication in insect disease vectors.

    abstract::Acoustic signalling has been extensively studied in insect species, which has led to a better understanding of sexual communication, sexual selection and modes of speciation. The significance of acoustic signals for a blood-sucking insect was first reported in the XIX century by Christopher Johnston, studying the hear...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/0074-0276130390

    authors: Vigoder Fde M,Ritchie MG,Gibson G,Peixoto AA

    更新日期:2013-01-01 00:00:00

  • Restriction fragment length polymorphism of 195 bp repeated satellite DNA of Trypanosoma cruzi supports the existence of two phylogenetic groups.

    abstract::The restriction fragment length polymorphism of the 195 bp repeated DNA sequence of Trypanosoma cruzi was analyzed among 23 T. cruzi stocks giving a reliable picture of the whole phylogenetic variability of the species. The profiles observed with the enzymes Hinf I and Hae III were linked together and supported the ex...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761999000300008

    authors: Bastrenta B,Bosseno MF,Barnabé C,Tibayrenc M,Brenière SF

    更新日期:1999-05-01 00:00:00

  • Phylogeny and evolution of the aspartyl protease family from clinically relevant Candida species.

    abstract::Aspartyl proteases are a class of enzymes that include the yeast aspartyl proteases and secreted aspartyl protease (Sap) superfamilies. Several Sap superfamily members have been demonstrated or suggested as virulence factors in opportunistic pathogens of the genus Candida. Candida albicans, Candida tropicalis, Candida...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000300018

    authors: Parra-Ortega B,Cruz-Torres H,Villa-Tanaca L,Hernández-Rodríguez C

    更新日期:2009-05-01 00:00:00

  • Reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures.

    abstract::This study aimed to correlate the presence of ica genes, biofilm formation and antimicrobial resistance in 107 strains of Staphylococcus epidermidis isolated from blood cultures. The isolates were analysed to determine their methicillin resistance, staphylococcal cassette chromosome mec (SCCmec) type, ica genes and bi...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276140120

    authors: Pinheiro L,Brito CI,Pereira VC,Oliveira Ad,Camargo CH,Cunha Mde L

    更新日期:2014-11-01 00:00:00

  • Inflammatory effects of snake venom metalloproteinases.

    abstract::Metalloproteinases are abundant enzymes in crotaline and viperine snake venoms. They are relevant in the pathophysiology of envenomation, being responsible for local and systemic hemorrhage frequently observed in the victims. Snake venom metalloproteinases (SVMP) are zinc-dependent enzymes of varying molecular weights...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02762005000900031

    authors: Teixeira Cde F,Fernandes CM,Zuliani JP,Zamuner SF

    更新日期:2005-03-01 00:00:00

  • The antimicrobial activity of lapachol and its thiosemicarbazone and semicarbazone derivatives.

    abstract::Lapachol was chemically modified to obtain its thiosemicarbazone and semicarbazone derivatives. These compounds were tested for antimicrobial activity against several bacteria and fungi by the broth microdilution method. The thiosemicarbazone and semicarbazone derivatives of lapachol exhibited antimicrobial activity a...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/S0074-02762013000300013

    authors: Souza MA,Johann S,Lima LA,Campos FF,Mendes IC,Beraldo H,Souza-Fagundes EM,Cisalpino PS,Rosa CA,Alves TM,de Sá NP,Zani CL

    更新日期:2013-05-01 00:00:00

  • Development of a cyanobacterial biolarvicide.

    abstract::Results of studies on a larvicidal cyanobacterium that expresses a Bti cryIVD gene fusion are reported. Genetically altered Agmenellum quadruplicatum PR-6 is shown to be toxic to larvae of three major genera of disease-bearing mosquitos. Factors affecting expression of Bti genes in cyanobacteria are discussed. ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761995000100021

    authors: Murphy RC,Stevens SE Jr

    更新日期:1995-01-01 00:00:00

  • Concurrent infection with dengue virus type-2 and DENV-3 in a patient from Ceará, Brazil.

    abstract::Dengue outbreaks have occurred in several regions in Brazil and cocirculating dengue virus type 1 (DENV-1), DENV-2, and DENV-3 have been frequently observed. Dual infection by DENV-2 and DENV-3 was identified by type-specific indirect immunofluorescence assay and confirmed by reverse transcription polymerase chain rea...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762006000800017

    authors: Araújo FM,Nogueira RM,de Araújo JM,Ramalho IL,Roriz ML,de Melo ME,Coelho IC

    更新日期:2006-12-01 00:00:00

  • Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon Region.

    abstract::We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000600016

    authors: Tang TH,López-Vélez R,Lanza M,Shelley AJ,Rubio JM,Luz SL

    更新日期:2010-09-01 00:00:00

  • Polymorphism in trypomastigotes of Trypanosoma (Megatrypanum) minasense in the blood of experimentally infected squirrel monkey and marmosets.

    abstract::Experimental infections by Trypanosoma (Megatrypanum) minasense were performed in primates - Saimiri sciureus and Callithrix penicillata - with the objective of searching for morphological variations of the blood trypomastigotes with respect to hosts and time of infection. We carried out morphological and morphometric...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761999000500016

    authors: Ziccardi M,Lourenco-de-Oliveira R

    更新日期:1999-09-01 00:00:00

  • Borrelia-like spirochetes recovered from ticks and small mammals collected in the Atlantic Forest Reserve, Cotia county, State of São Paulo, Brazil.

    abstract::Forty-four marsupials, 77 rodents and 161 ticks were captured in an Atlantic Forest Reserve in Cotia county, State of São Paulo, where human cases of Lyme disease (LD) simile were reported. Twenty-one borrelia-like spirochete isolates were recovered from the mammals' blood and rodent livers or spleens, and triturated ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000500006

    authors: Abel IS,Marzagão G,Yoshinari NH,Schumaker TT

    更新日期:2000-09-01 00:00:00

  • [Production and standardization of Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus antigens to be used in immunodiagnosis. Comparison between immunodiffusion and immunoelectrophoresis].

    abstract::Soluble antigens (Ag) from Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus were prepared and standardized by double immunodiffusion (DID) and immunoelectroosmophoresis (IEOP). No difference in sensitivity was observed between the two techniques; 100% of standard patient sera were positi...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761985000300005

    authors: Ferreira-da-Cruz MF,Galvão-Castro B,Wanke B

    更新日期:1985-07-01 00:00:00

  • Vascular dysfunction as a target for adjuvant therapy in cerebral malaria.

    abstract::Cerebral malaria (CM) is a life-threatening complication of Plasmodium falciparum malaria that continues to be a major global health problem. Brain vascular dysfunction is a main factor underlying the pathogenesis of CM and can be a target for the development of adjuvant therapies for the disease. Vascular occlusion b...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/0074-0276140061

    authors: Carvalho LJ,Moreira Ada S,Daniel-Ribeiro CT,Martins YC

    更新日期:2014-08-01 00:00:00

  • Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil.

    abstract:BACKGROUND:Enterocytozoon bieneusi are the most common microsporidia associated with different clinical manifestations such as diarrhoea, respiratory tract inflammation and acalculous cholecystitis, especially in immunocompromised patients. Infection usually occurs by ingestion of food and water contaminated with spore...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160435

    authors: Yamashiro S,Fiuza VRDS,Teixeira ÂTLS,Branco N,Levy CE,Castro ICVS,Franco RMB

    更新日期:2017-06-01 00:00:00

  • Preliminary analysis of Sm14 in distinct fractions of Schistosoma mansoni adult worm extract.

    abstract::In previous studies it was shown that the recombinant molecule, r-Sm14, induces high levels of protection against Schistosoma mansoni infection in two outbred animal models and immune crossprotection against infection by Fasciola hepatica in Swiss outbred mice. r-Sm14 was derived from a living worm extract, called SE,...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000900011

    authors: Thaumaturgo N,Vilar MM,Diogo CM,Edelenyi R,Tendler M

    更新日期:2001-01-01 00:00:00

  • Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro.

    abstract:BACKGROUND:Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE:Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS:L. braziliensis-GFP was generated using Leishmania tarentolae pLEXS...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160237

    authors: Bastos MS,Souza LÂ,Onofre TS,Silva A Júnior,Almeida MR,Bressan GC,Fietto JL

    更新日期:2017-02-01 00:00:00

  • Increased hepcidin expression in multibacillary leprosy.

    abstract::Iron is essential for all organisms and its availability can control the growth of microorganisms; therefore, we examined the role of iron metabolism in multibacillary (MB) leprosy, focusing on the involvement of hepcidin. Erythrograms, iron metabolism parameters, pro-inflammatory cytokines and urinary hepcidin levels...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000900026

    authors: Souza VN,Malaspina TS,Campanelli AP,Ghidella C,Ura S,Dalpino D,do Nascimento DC,Latini AC

    更新日期:2012-12-01 00:00:00

  • Protective CD8+ T cell responses against the pre-erythrocytic stages of malaria parasites: an overview.

    abstract::CD8+ T cells have been implicated as critical effector cells in protection against the pre-erythrocytic stage of malaria in mice and humans following irradiated sporozoite immunization. Immunization experiments in animal models by several investigators have suggested different strategies for vaccination against malari...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02762001000200014

    authors: Oliveira-Ferreira J,Daniel-Ribeiro C

    更新日期:2001-02-01 00:00:00

  • Diagnostic challenges of single plaque-like lesion paucibacillary leprosy.

    abstract::The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276140212

    authors: Barbieri RR,Sales AM,Illarramendi X,Moraes MO,Nery JA,Moreira SJ,Sarno EN,Machado Ade M,Bozza FA

    更新日期:2014-11-01 00:00:00

  • Association of NR1I2 gene polymorphisms and time of progression to AIDS.

    abstract:BACKGROUND:The time of progression towards AIDS can vary greatly among seropositive patients, and may be associated with host genetic variation. The NR1I2 (PXR) gene, a ligand-activated transcription factor, regulates the transcription immune pathway genes and can therefore be targets of viral replication mechanisms in...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160382

    authors: Medeiros RM,Menti CF,Benelli JL,Matte MC,Melo MG,Almeida SE,Fiegenbaum M

    更新日期:2017-04-01 00:00:00

  • Distribution and Schistosoma mansoni infection of Biomphalaria glabrata in different habitats in a rural area in the Jequitinhonha Valley, Minas Gerais, Brazil: environmental and epidemiological aspects.

    abstract::This paper examines the distribution and infection of Biomphalaria glabrata with Schistosoma mansoni in all aquatic snail habitats in a rural area in the state of Minas Gerais, Brazil, in relation to physico/biotic and behavioral factors. Snail and environmental surveys were carried out semi-annually between July 2001...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762004000700002

    authors: Kloos H,Passos LK,Loverde P,Oliveira RC,Gazzinelli A

    更新日期:2004-11-01 00:00:00

  • A chromosome 9 deletion in Plasmodium falciparum results in loss of cytoadherence.

    abstract::Many lines of Plasmodium falciparum undergo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and the selected cells produce gametocy...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761992000700011

    authors: Kemp DJ,Thompson J,Barnes DA,Triglia T,Karamalis F,Petersen C,Brown GV,Day KP

    更新日期:1992-01-01 00:00:00

  • TcruziDB, an integrated database, and the WWW information server for the Trypanosoma cruzi genome project.

    abstract::Data analysis, presentation and distribution is of utmost importance to a genome project. A public domain software, ACeDB, has been chosen as the common basis for parasite genome databases, and a first release of TcruziDB, the Trypanosoma cruzi genome database, is available by ftp from ftp://iris.dbbm.fiocruz.br/pub/g...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761997000600015

    authors: Degrave W,de Miranda AB,Amorim A,Brandão A,Aslett M,Vandeyar M

    更新日期:1997-11-01 00:00:00

  • Ecogenetics of Triatoma sordida and Triatoma guasayana (Hemiptera: reduviidae) in the Bolivian chaco.

    abstract::Triatoma guasayana and two putative cryptic species pertaining to T. sordida complex (named groups 1 and 2) occur in sympatry in the Bolivian Chaco. Using multilocus enzyme electrophoresis and subsequent genetic analysis, our work assesses their population distribution and dispersal capacity in domestic, peridomestic,...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/S0074-02761999000400004

    authors: Noireau F,Gutierrez T,Flores R,Breniere F,Bosseno MF,Wisnivesky-Colli C

    更新日期:1999-07-01 00:00:00

  • Efficiency of diagnostic biomarkers among colonic schistosomiasis Egyptian patients.

    abstract::The schistosomal parasite plays a critical role in the development of malignant lesions in different organs. The pathogenesis of cancer is currently under intense investigation to identify reliable prognostic indices for disease detection. The objective of this paper is to evaluate certain biochemical parameters as di...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000300011

    authors: Hamed MA,Ahmed SA,Khaled HM

    更新日期:2011-05-01 00:00:00

  • Leishmania (Viannia) naiffi: rare enough to be neglected?

    abstract::In the Brazilian Amazon, American tegumentary leishmaniasis (ATL) is endemic and presents a wide spectrum of clinical manifestations due, in part, to the circulation of at least seven Leishmania species. Few reports of Leishmania (Viannia) naiffi infection suggest that its occurrence is uncommon and the reported cases...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760150128

    authors: Fagundes-Silva GA,Romero GA,Cupolillo E,Yamashita EP,Gomes-Silva A,Guerra JA,Da-Cruz AM

    更新日期:2015-09-01 00:00:00

  • COI barcode versus morphological identification of Culex (Culex) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil.

    abstract::Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex (Culex) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276130457

    authors: Laurito M,Oliveira TM,Almirón WR,Sallum MA

    更新日期:2013-01-01 00:00:00

  • Evaluation of adherence, hemagglutination, and presence of genes codifying for virulence factors of Acinetobacter baumannii causing urinary tract infection.

    abstract::Acinetobacter baumannii is a strictly aerobic bacterium which causes severe infections, however its pathogenic characteristics are not well defined. Thirteen A. baumannii strains isolated from urine of hospitalized and nonhospitalized patients with different ages were investigated for the presence of virulence factors...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762004000800010

    authors: Braun G,Vidotto MC

    更新日期:2004-12-01 00:00:00